Insights from expert elicitation: prioritising pathogens for genome sequencing for Australian public health responseResearch in context by Tehzeeb Zulfiqar, Angeline Ferdinand, Danielle Cribb, Patiyan Andersson, Alireza Zahedi, Kathryn Glass, Son Nghiem, Susan Trevenar, Nhung Mai, Benjamin P. Howden, Martyn D. Kirk

“…Experts agreed that a national priority pathogen list for genome sequencing should be developed collaboratively with public health laboratories and complemented by state level lists, with biennial reviews and flexibility for situation-based adjustments. …”
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Uncovering the Multifaceted Role of <i>PA2649</i> (<i>nuoN</i>) in Type III Secretion System and Other Virulence Production in <i>Pseudomonas aeruginosa</i> PAO1 by Lin Chen, Yujie Si, Xue Han, Yue Xiao, Yidan Pan, Kangmin Duan, Songzhe Fu

“…These phenotypes could not be complemented with T3SS regulatory genes alone, indicating that <i>PA2649</i> modulates these traits through mechanisms independent of the Rsm-Exs axis, potentially involving NADH dehydrogenase-associated pathways. …”
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HpFBH3 transactivates HpCO7 via binding to the E-boxes in the promoter and may accelerate flower formation in pitaya by Xiaowei Cai, Ling Xiao, Xiangmei Nie, Qiandong Hou, Sulin Wen, Kun Yang, Xiaopeng Wen

“…HpFBH3 was found to heterodimerize with HpFBH1 in the nucleus using subcellular localization, yeast two-hybrid and luciferase complementation assays. With bioinformatic analysis, all HpFBHs were predicted to transactivate downstream genes via binding to the E-boxes, which were frequently detected in the promoters of HpCOs, HpFTs and HpSOC1s. …”
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Non-Ideal Hall MHD Rayleigh–Taylor Instability in Plasma Induced by Nanosecond and Intense Femtosecond Laser Pulses by Roman S. Zemskov, Maxim V. Barkov, Evgeniy S. Blinov, Konstantin F. Burdonov, Vladislav N. Ginzburg, Anton A. Kochetkov, Aleksandr V. Kotov, Alexey A. Kuzmin, Sergey E. Perevalov, Il’ya A. Shaikin, Sergey E. Stukachev, Ivan V. Yakovlev, Alexander A. Soloviev, Andrey A. Shaykin, Efim A. Khazanov, Julien Fuchs, Mikhail V. Starodubtsev

“…The experimental results are complemented by and consistent with numerical 3D magnetohydrodynamic simulations. …”
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Association between myopia and diabetic retinopathy: A two-sample mendelian randomization study by Jinyi Xu, Shengsong Xu, Xiao Wang, Chuqi Xiang, Zhenbang Ruan, Mingxin Lu, Liying He, Yin Hu, Xiao Yang

“…The inverse variance weighted (IVW) method was mainly used to assess the causality, and was complemented with sensitivity analyses and causality direction analyses. …”
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Environmental pollution and human fertility: investigating the relationship between PM2.5 exposure and assisted reproductive technology outcomes by Fei Li, Xiaoyan Duan, Mingming Li, Yumei Gao, Yuejuan Kang, WenJun Zheng, XueYan Guo, Ying Chen

“…Conclusions The study complements existing evidence that exposure to PM2.5 can lead to decreased success rates of pregnancy and live births, as well as significantly impact the outcomes of ART. …”
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Strengthening the WHO Emergency Care Systems Framework: insights from an integrated, patient-centered approach in the Copenhagen Emergency Medical Services system—a qualitative sys... by Simone Böbel, Jeske Verhoeven, Mirjam Scholz, Bart Penders, Lorraine Frisina Doetter, Helle Collatz Christensen, Thomas Krafft

“…These include education and citizen participation programs, early triaging, differentiated care pathways coordinated with primary care and out-of-hours services, and specialized mobile care units complementing “traditional” ambulance services. Technology supports integrated and patient-centered care by facilitating early differentiation of care, efficient dispatching, and communication. …”
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Predictive value of the systemic inflammation grade for overall survival in patients with colorectal cancer after surgery: outperforming NLR and mGPS by Jianing Wang, Yujun Liu, Wenliang Jiang, Dongli Zhang, Chao Cheng, Cuixia Liu, Zhibin Zhao, Honggang Wang

“…Subgroup analysis showed consistent prognostic efficacy of SIG in both colon and rectal cancers, with no significant interaction between SIG and tumor location (P=0.309).ConclusionsSIG outperforms existing biomarkers and complements TNM staging by capturing systemic inflammation-driven risk heterogeneity. …”
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Reliable detection of CNS lymphoma-derived circulating tumor DNA in cerebrospinal fluid using multi-biomarker NGS profiling: insights from a real-world study by Veronika Navrkalova, Andrea Mareckova, Samuel Hricko, Viera Hrabcakova, Lenka Radova, Vaclav Kubes, Jakub Porc, Tomas Reigl, Sarka Pospisilova, Jana Kotaskova, Andrea Janikova

“…Less invasive liquid biopsy testing by comprehensive NGS complements standard procedures in the diagnostics and management of CNSL patients, especially when encountering limitations.…”
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Transversal Competencies in Operating Room Nurses: A Hierarchical Task Analysis by Francesca Reato, Dhurata Ivziku, Marzia Lommi, Alessia Bresil, Anna Andreotti, Chiara D’Angelo, Mara Gorli, Mario Picozzi, Giulio Carcano

“…<b>Conclusions:</b> This framework complements the existing repertoire of technical-specialist competencies by integrating essential transversal competencies. …”
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AI Efficiency in Dentistry: Comparing Artificial Intelligence Systems with Human Practitioners in Assessing Several Periodontal Parameters by Oana-Maria Butnaru, Monica Tatarciuc, Ionut Luchian, Teona Tudorici, Carina Balcos, Dana Gabriela Budala, Ana Sirghe, Dragos Ioan Virvescu, Danisia Haba

“…<i>Conclusions:</i> AI systems exhibit significant potential as reliable tools for dental and periodontal assessment, complementing the expertise of human practitioners. However, further validation in clinical settings is necessary to address limitations such as algorithmic bias and atypical cases. …”
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Individual variation in migration patterns of Iberian little bustards by Filipa Coutinho Soares, João Gameiro, Ana Teresa Marques, Gerard Bota, Eládio L. García de la Morena, Manuel B. Morales, Carlos Pacheco, David González del Portillo, Francesco Valerio, Francisco Moreira, Jorge M. Palmeirim, João Paulo Silva

“…This approached was then complemented with traditional linear mixed models on the daily ND values throughout the year. …”
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Serum cystatin C as a potential biomarker for generalized acetylcholine receptor antibody-positive myasthenia gravis by Dingxian He, Huahua Zhong, Lei Jin, Ran Chen, Zongtai Wu, Rui Zhao, Xiao Huan, Chong Yan, Jie Song, Jianying Xi, Chongbo Zhao, Shanfeng Zhu, Shanfeng Zhu, Shanfeng Zhu, Sushan Luo

“…We assessed cytokines/chemokines/complements and peripheral blood T lymphocytes using Luminex assays and flow cytometry. …”
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Blood RNA-seq in rare disease diagnostics: a comparative study of cases with and without candidate variants by Xiaomei Luo, Bing Xiao, Lili Liang, Kaichuang Zhang, Ting Xu, Huili Liu, Yi Liu, Yongguo Yu, Yanjie Fan

“…Blood RNA sequencing (RNA-seq) complements DNA-level diagnosis by revealing the functional impact of variants on gene expression and splicing, but to what extent RNA-driven approaches offer diagnostic benefits across different scenarios—with and without pre-existing candidate variants—remains uncertain. …”
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Evaluation of low-cost techniques to detect sickle cell disease and β-thalassemia: an open-label, international, multicentre studyResearch in context by Pranav Shrestha, Hendrik Lohse, Christopher Bhatla, Heather McCartney, Alaa Alzaki, Navdeep Sandhu, Pardip Kumar Oli, Sanjeev Chaudhary, Ali Amid, Rodrigo Onell, Nicholas Au, Hayley Merkeley, Videsh Kapoor, Rajan Pande, Boris Stoeber

“…However, HemotypeSC and Sickle SCAN miss β-thalassemia trait, and need to be complemented with other low-cost tests. Funding: UBC PSI, Canada Research Chairs, UBC HIFI Awards, UBC 4YF, Naiman Vickars Endowment fund.…”
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Water-Soluble Cu(II) Complexes with Polypyridyl Ligands: Anticancer Activity and DNA Interaction by Herisson F. dos Santos, Nádija N. P. da Silva, George B. S. Pereira, Mauro A. Lima, Nailton M. Nascimento-Júnior, Renan L. de Farias, Amos O. Akinyemi, Fillipe V. Rocha

“…The interaction of the complexes with DNA was evaluated through an ethidium bromide displacement assay, complemented by theoretical studies using molecular docking. …”
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Decreased brain-derived neurotrophic factor expression in chronic kidney disease: integrated clinical and experimental evidence by Juan Chen, Lili Fu, Mengjin Li, Kun Xie, Xinming Li, Xu-jie Zhou, Li Yang, Liming Zhang, Cheng Xue, Cheng Xue, Zhiguo Mao

“…However, the evidence and regulatory mechanisms underlying decreased BDNF levels in CKD remain inconclusive.MethodsThis study systematically evaluated circulating BDNF alterations in CKD patients through a meta-analysis of clinical studies involving 1,549 participants, complemented by experimental validation in unilateral ureteral obstruction (UUO) mice and single-cell transcriptomic database analysis to investigate tissue-specific BDNF protein expression and regulatory patterns.ResultsMeta-analysis confirmed significantly reduced circulating BDNF in CKD patients (WMD = −0.62 ng/mL, 95% CI [-0.98, −0.25], P &lt; 0.001; I2 = 87%). …”
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Factors influencing patients’ willingness to share their digital health data for primary and secondary use: A theory- and evidence-based overview of reviews by Sabrina Fesl, Caroline Lang, Jochen Schmitt, Stefanie Brückner, Stephen Gilbert, Stefanie Deckert, Madlen Scheibe

“…Methods The MEDLINE, Embase, and Scopus literature databases were searched in June 2023, complemented by additional searches, to identify SRs focusing on the determinants of patients’ willingness to share HD published from 2013 to 2023. …”
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NO₂ Air Pollution Trends and Settlement Growth in Megacities by Thilo Erbertseder, Hannes Taubenbock, Thomas Esch, Lorenza Gilardi, Heiko Paeth, Stefan Dech

“…Despite this variety, the results exhibit a pronounced relation to the income group following the world&#x0027;s economies classification of the World Bank. Complemented by a cluster analysis and ranking of the megacities, the study provides indicators to develop a better understanding of the drivers and impacts of urbanization and air pollution.…”
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Dissecting the genetic basis and mechanisms underlying the associations between multiple extrahepatic factors and autoimmune liver diseases by Zheng Zhang, Jiayi Zhang, Xinyang Yan, Jiachen Wang, Haoxiang Huang, Menghao Teng, Qingguang Liu, Shaoshan Han

“…Mendelian randomization (MR) was employed to assess the causal effects of these extrahepatic factors on AILDs, complemented by linkage disequilibrium score (LDSC) regression to uncover shared genetic architecture and causal effects underlying the associations between autoimmune diseases. …”
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