Generation of Cas9 Knock-In <i>Culex quinquefasciatus</i> Mosquito Cells by Elizabeth Walsh, Tran Zen B. Torres, Brian C. Prince, Claudia Rückert

“…Here, we established a monoclonal cell line that consistently expresses Cas9 and can be used for screens to determine gene function or antiviral activity. <b>Methods</b>: We used this system to perform the successful gene editing of seven genes and subsequent testing for potential antiviral effects, using a simple single-guide RNA (sgRNA) transfection and subsequent virus infection. …”
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Dynamic Monitoring of Goaf Stress Field and Rock Deformation Driven by Optical Diber Sensing Technology by Jing Chai, Zhe Yan, Yibo Ouyang, Dingding Zhang, Jianfeng Yang, Gaoyi Yang, Chenyang Ma

“…The developed stress inversion formula successfully predicted pressure distribution patterns within the goaf, achieving real-time monitoring capabilities. …”
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Patient and public involvement in the co-design and assessment of unobtrusive sensing technologies for care at home: a user-centric design approach by Jenny Sharma, Nazia Gillani, Imran Saied, Aaesha Alzaabi, Tughrul Arslan

“…Suggestions for refining the prototypes included adding functionality for detecting deviations in daily routines and integrating feedback mechanisms for caregivers. …”
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Long-Term Therapeutic Effects of ²²⁵Ac-DOTA-E[c(RGDfK)]₂ Induced by Radiosensitization via G2/M Arrest in Pancreatic Ductal Adenocarcinoma by Mitsuyoshi Yoshimoto, Kohshin Washiyama, Kazunobu Ohnuki, Ayano Doi, Miki Inokuchi, Motohiro Kojima, Brian W. Miller, Yukie Yoshii, Anri Inaki, Hirofumi Fujii

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Multi-Omics Analysis Provides Insights into Green Soybean in Response to Cold Stress by Yanhui Lin, Guangping Cao, Jing Xu, Honglin Zhu, Liqiong Tang

“…The DEGs and DEMs were further subjected to KEGG functional analysis. Finally, 11 metabolites (such as sucrose, lactose, melibiose, and dehydroascorbate) and 17 genes (such as <i>GOLS</i>, <i>GLA</i>, <i>UGDH</i>, and <i>ALDH</i>) were selected as candidates associated with cold tolerance. …”
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Effects of Debittering Treatments on Some Secondary Metabolites’ Bioaccessibility, Fatty Acid, and Aroma Profiles of Semidried Gemlik Variety Black Olives by Senanur Durgut Malçok, Cüneyt Tunçkal, Azime Özkan Karabacak, Gülderen Coşgun, Mehmet Torun, Canan Ece Tamer

“…In the evaluation of the fatty acid composition of raw and table olives, 11 different fatty acids including oleic, palmitic, linoleic, stearic, elaidic, palmitoleic, and arachidic were detected in all samples. A total of 57 aroma compounds were detected in the samples, of which 55 were successfully identified. …”
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Item-level measurement properties of the pediatric awareness and sensory motor assessment in children with medical complexity by Brooke Mulrenin, Bryant A. Seamon, Carrie Cormack, Kimberly Lane Kascak, Cynthia Dodds

“…Current assessment tools fail to detect subtle abilities in CMC, hindering development of effective rehabilitation goals and interventions. …”
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Bertolotti Syndrome: Does it really exist? systematic review by Acevedo-Gonzalez Juan Carlos, Delgado-Caicedo Mónica Gabriela, Lacouture-Silgado Isabella

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High-Sensitivity Determination of K, Ca, Na, and Mg in Salt Mines Samples by Atomic Emission Spectrometry with a Miniaturized Liquid Cathode Glow Discharge by Jie Yu, Zhichao Zhang, Quanfang Lu, Duixiong Sun, Shuwen Zhu, Xiaomin Zhang, Xing Wang, Wu Yang

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Identification of a Rare Mutation in the SRD5A2 Gene in an Iranian Family with Sex Development Disorder by Atiyeh Ataei, Peyman Eshraghi, Atiyeh Eslahi, Zeinab Khazaei, Masoome Alerasool, Sara Jamali, Faranak Ghadamyari, Majid Mojarrad

“…According to WES result and Sanger sequencing a homozygote loss of function mutation (c.16C>T; p.Gln6Ter) in SRD5A2 has been detected. …”
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Synthesis of 2-Amino-4, 5-Diarylthiazole Derivatives and Evaluation of Their Anti-<i>Candida</i> Albicans Activity by Dongmei Gao, Lele Shi, Yuhang Huang, Yingmei Lv, Xuan Yang, Zhenting Du

“…Thus, <b>5a8</b> was synthesized successfully, giving anti-<i>Candida albicans</i> activity (MIC₈₀ = 9 μM) similar to that of a typical antifungal drug, fluconazole. …”
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History and prospects of flax genetic markers by Daiana A. Zhernova, Elena N. Pushkova, Tatiana A. Rozhmina, Elena V. Borkhert, Alexander A. Arkhipov, Alexander A. Arkhipov, Elizaveta A. Sigova, Elizaveta A. Sigova, Ekaterina M. Dvorianinova, Ekaterina M. Dvorianinova, Alexey A. Dmitriev, Alexey A. Dmitriev, Nataliya V. Melnikova

“…Flax (Linum usitatissimum L.) is known as a dual-purpose crop, producing both fiber and oil, which have a wide range of uses. Successful flax breeding requires knowledge on the genetic determinants of flax traits. …”
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Quantitative Proteomics Revealed the Molecular Regulatory Network of Lysine and the Effects of Lysine Supplementation on Sunit Skeletal Muscle Satellite Cells by Mingxu Wang, Fan Bai, Qinan Zhao, Jianan Shi, Yutian Hao, Jindi Wu

“…This study explored lysine (Lys)’s effects on SMSCs and their protein profiles in Sunit sheep. SMSCs were successfully isolated, assessing their survival and proliferation after Lys stimulation at varying concentrations using the CCK-8 assay. …”
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Scaling-up of carbon dots hydrothermal synthesis from sugars in a continuous flow microreactor system for biomedical application as in vitro antimicrobial drug nanocarrier by Siriboon Supajaruwong, Sirawich Porahong, Agung Wibowo, Yu-Sheng Yu, Mohd Jahir Khan, Pisut Pongchaikul, Pattaraporn Posoknistakul, Navadol Laosiripojana, Kevin C.-W. Wu, Chularat Sakdaronnarong

“…Carbon dots (CDs) are a new class of nanomaterials exhibiting high biocompatibility, water solubility, functionality, and tunable fluorescence (FL) property. …”
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A comparative analysis of single-step and multi-step methods for producing magnetic activated carbon from palm kernel shells: Adsorption of methyl orange dye by Waluyo Joko, Rahmawati Farida Dwi, Izzulhaq Muhammad Ghozy, Purba Ibnu Tryansar, Kaavessina Mujtahid, Wibowo Wusana Agung, Pranolo Sunu Herwi, Buwono Haris Puspito, Septian Ardie, Adnan Muflih Arisa

“…Fourier transform infra-red characterization showed that the functional groups detected on the activated carbon and MAC were O–H, C═O, C═C, C≡N, and C–O. …”
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Age- and Post-Based Complex Analyses of Heart Rate Variability in Young Male Handball Players for Potential Prevention of Overload-induced Injuries by Zita Petrovszki, Olivér Czimbalmos, Vera Gal, Gabor Korosi, Edit Nagy, Rita Mikulan, Gyongyi Horvath

“…Anthropometric parameters, HR, R-R interval (RRi), root mean square of successive difference of RRi (RMSSD), and its corrected values (by division or multiply with powers of RRi; C_RMSSDs) were analyzed. …”
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ClimKern v1.2: a new Python package and kernel repository for calculating radiative feedbacks by T. P. Janoski, T. P. Janoski, T. P. Janoski, T. P. Janoski, T. P. Janoski, I. Mitevski, R. J. Kramer, M. Previdi, L. M. Polvani, L. M. Polvani, L. M. Polvani

“…<p>Climate feedbacks are a significant source of uncertainty in future climate projections and need to be quantified accurately and robustly. …”
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Exploring Nonlinear Threshold Effects and Interactions Between Built Environment and Urban Vitality at the Block Level Using Machine Learning by Cong Li, Yajuan Zhou, Manfei Wu, Jiayue Xu, Xin Fu

“…This study proposed to develop a comprehensive framework for urban vitality by incorporating multi-source data, and the central urban area of Xi’an, China, was selected as the study area. …”
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Isolation and Screening of Lactic Acid Bacteria From Sumbawa Buffalo Milk (Bubalus bubalis) as Potential Starter Cultures by Deni Harmoko, Tri Ardyati, Yoga Dwi Jatmiko

“…Besides containing nutrients that are very beneficial for human health, buffalo milk is also a potential source of lactic acid bacteria (LAB) with technological and functional properties. …”
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ECONOMIC ASPECTS OF ORGANIC SOY PRODUCTION IN UKRAINE by Hanna Pantsyreva

“…It has been proven that a genuine source of organic matter for enhancing soil fertility is the development of organic models of soybean cultivation technology. …”
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