Determination of malachite green in sediment by ultra performance liquid chromatography-mass spectrometry by Mei Guangming, Guo Yuanming, Zhang Xiaojun, Yan Zhongyong, Li Peipei, You Juju, Ding Yueping

“…Due to the matrix complex of sediment, sample pretreatment technology is the key technology. As a new type of functional material, mut-i walled nanotubes (MWCNTs) have extensive prospect in the field of analytical chemistry. …”
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Application of Concentration-Area fractal modeling and artificial neural network to identify Cu, Zn±Pb geochemical anomalies in Hashtjin area, NW of Iran by Ali Imamalipour, Hamed Ebrahimi, Amir reza Abdollahpur

“…Autoencoder consists of two functions: an encoder function that encodes the input data into a lower-dimensional hidden layer and a decoder function that reconstructs the encoded input data. …”
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Genome and Pathogenicity Analysis of an NADC30-like PRRSV Strain in China’s Xinjiang Province by Honghuan Li, Wei Zhang, Yanjie Qiao, Wenxing Wang, Wenxiang Zhang, Yueli Wang, Jihai Yi, Huan Zhang, Zhongchen Ma, Chuangfu Chen

“…In this study, we successfully isolated a novel strain named XJ-Z5 from PRRSV-positive samples collected in Xinjiang province in 2022. …”
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Cytotoxicity to cells revealed the virulence-associated protein E gene as a potential virulence factor in methicillin-resistant Staphylococcus aureus by Dengyuan Li, Yu Shi, Ning Zou, Tingting Yang, Wenpeng Gu, Feng Liao

“…The aim of this study was to explore the structure and function of the virE gene of MRSA, and to provide a basis for subsequent in-depth study of this gene. …”
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The Experience of Using a Combination of Febuxostat and Canakinumab in Tophaceous Gout Complicated by Chronic Kidney Disease in a Teenage Patient: A Case Study by Maria I. Kaleda, Irina P. Nikishina, Nurali Z. Zokirov, Alina V. Charlamova, Maxim S. Eliseev

“…Late initiation of urate-lowering therapy in children with gout increases the risk of severe disability due to the state of the musculoskeletal system and kidney function. Description of a Clinical Case. At the age of 13 years and 9 months, the boy first experienced acute pain, hyperthermia, and restriction of movement in the right elbow joint. …”
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Epithelial-to-mesenchymal transition (EMT) and cancer metastasis: the status quo of methods and experimental models 2025 by Heike Allgayer, Samikshya Mahapatra, Barnalee Mishra, Biswajit Swain, Suryendu Saha, Sinjan Khanra, Kavita Kumari, Venketesh K. Panda, Diksha Malhotra, Nitin S. Patil, Jörg H. Leupold, Gopal C. Kundu

“…Metastasis still needs to be better understood functionally in its major but complex steps of migration, invasion, intravasation, dissemination, which contributes to the establishment of minimal residual disease (MRD), extravasation, and successful seeding and growth of metastatic lesions at microenvironmentally heterogeneous sites. …”
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Uncovering the role of Gpr45 in obesity regulation by Eva O. Karolczak, Chia Li, Ivan C. Alcantara, Isabel M. Cohen, Claire Gao, Cuiying Xiao, Abigail I. Goldschmidt, Cynthia A. Pinkus, Junjie Li, Monica M. Li, Ryan M. Esquejo, Jean-Philippe Fortin, Kendra K. Bence, Marc L. Reitman, Michael J. Krashes

“…Underscoring this, agonists for GPCRs have recently revolutionized the treatment of diabetes and obesity. The rampant success of these compounds has invigorated interest in identifying additional GPCRs that modulate appetite and body weight homeostasis. …”
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Viable but nonculturable state in the zoonotic pathogen Bartonella henselae induced by low-grade fever temperature and antibiotic treatment by Yuze Gou, Yuze Gou, Dongxia Liu, Dongxia Liu, Yuxian Xin, Yuxian Xin, Ting Wang, Jiaxin Li, Yiwen Xi, Xiaoling Zheng, Tuanjie Che, Ying Zhang, Ying Zhang, Tingting Li, Jie Feng, Jie Feng

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Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity by Yingying Huang, Qiqi Mao, Jian He, Jing Su, Yi Peng, Wei Liang, Zixi Hu, Sufang Zhou, Xiaoling Lu, Yongxiang Zhao

“…Purified ECs (CD105+) took the function of endothelial cells, then successfully fused with DCs. …”
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Gut Microbiota Modulation by Lysozyme as a Key Regulator of Vascular Inflammatory Aging by Chenyang Zhang, Xin Zhao, Hang Zhang, Tongtong Wang, Zhenyu Zhang, Yilin Yin, Hui Wang, Xiao Tong, Yuzheng Xue, Yahong Zhou, Fenglai Yuan, Xiuwu Bian, Hong Wei, Yuan Huang, Tianhao Liu

“…Lyz1 deficiency led to vascular inflammatory aging, including damage to indicators related to oxidative stress, vascular function, and inflammation in the serum and vascular tissues of wild-type (WT) and Lyz1−/− mice. …”
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Identification of a Rare Mutation in the SRD5A2 Gene in an Iranian Family with Sex Development Disorder by Atiyeh Ataei, Peyman Eshraghi, Atiyeh Eslahi, Zeinab Khazaei, Masoome Alerasool, Sara Jamali, Faranak Ghadamyari, Majid Mojarrad

“…According to WES result and Sanger sequencing a homozygote loss of function mutation (c.16C>T; p.Gln6Ter) in SRD5A2 has been detected. …”
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Hepatic epithelioid hemangioendothelioma, a rare liver tumor: a case report and review of the literature by Chao Deng, Yulong An, Yulong An, Jinli Liu, Jinli Liu, Chao Wang, Haoxian Gou, Jinpeng Zhang, Minghui Gu, Mengmeng Li, Tao Wang, Hao Luo

“…Multiple space-occupying lesions in the liver were incidentally detected through ultrasound imaging, despite the patient being asymptomatic. …”
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Rapid and sensitive determination of bongkrekic acid with molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry by Chunfei Zhong, Xinyan Li, Fan Zhang, Ning Liu, Jiewei Deng, Yunyun Yang, Tiangang Luan

“…The practical application of the method was confirmed through the successful analysis of real-world samples, including tap water, wastewater from a food processing factory, and the aqueous extracts of food samples, with BA detected at 0.296 µg/L in Tremella. …”
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Validation of a comprehensive long-read sequencing platform for broad clinical genetic diagnosis by Siddhartha Sen, Hillary P. Handler, Alec Victorsen, Zach Flaten, Aidan Ellison, Todd P. Knutson, Sarah A. Munro, Ryan J. Martinez, Charles John Billington, Jennifer J. Laffin, Sarah Bray, Pawel Mroz, Sophia Yohe, Andrew C. Nelson, Matthew Bower, Bharat Thyagarajan

“…Our findings suggest that long-read sequencing is successful in identifying diverse genomic alterations and that our pipeline functions well as the basis for a single diagnostic test for patients with suspected genetic disease.…”
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Data‐Driven Theoretical Modeling of Centrifugal Step Emulsification and Its Application in Comprehensive Multiscale Analysis by Xin Wang, Xiaolu Cai, Chao Wan, Huijuan Yuan, Shunji Li, Yiwei Zhang, Ran Zhao, Yuxi Qin, Yiwei Li, Bi‐Feng Liu, Peng Chen

“…This predictive model empowers the CASE to achieve all‐in‐one functionality, including droplet pre‐design, generation, manipulation, and on‐site detection. …”
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Impact of non-invasive oxygen reserve index versus standard SpO2 monitoring on peripheral oxygen saturation during endotracheal intubation in the intensive care unit: Protocol for... by Hugo Hille, Aurélie Le Thuaut, Pierre Asfar, Quentin Quelven, Emmanuelle Mercier, Anthony Le Meur, Jean-Pierre Quenot, Virginie Lemiale, Grégoire Muller, Martin Cour, Alexis Ferré, Asael Berge, Anaïs Curtiaud, Maxime Touron, Gaetan Plantefeve, Jean-Charles Chakarian, Jean-Damien Ricard, Gwenhael Colin, Arthur Orieux, Patrick Girardie, Mathieu Jozwiak, Manon Rouaud, Camille Juhel, Jean Reignier, Jean-Baptiste Lascarrou, CRICS-TRIGGERSEP Network

“…The primary outcome is the lowest SpO2 value from laryngoscopy to 2 minutes after successful ETI. Secondary outcomes include cognitive function on day 28. …”
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GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia by Lai L, Wu H, Peng L, Zhang Z, Wu X, Zheng S, Su Z, Chu H

“…A potential underlying mechanism could be that the autophagy induced by AST increases eNOS activity.Conclusion: GelMA@LNP/AST enables complete revascularization in female mice after hind limb ischemia, thereby achieving limb preservation and restoring motor function. Given the good therapeutic potential of the GelMA@LNP/AST in revascularization, it may become an effective strategy for successfully salvaging limbs in cases of limb ischemia.Keywords: astilbin, endothelial cells, autophagy, eNOS, angiogenesis…”
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Cloning and Analysis of the Expansin Gene BdEXP1 from Migration Plant parasitic Nematode (Bursaphelenchus doui) by Kaiyang GUO, Shifeng LIN, Rengang WANG, Li LI, Jie ZHANG, Shizhou YU, Zili WANG, Guoqin LIU

“…【Objective】An expansin gene BdEXP1 was cloned from Bursaphelenchus doui, and its expression characteristics were investigated in order to provide reference for the in-depth study of the function of BdEXP1 gene in the interactions between B. doui and hosts.…”
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Morning versus Nocturnal Heart Rate and Heart Rate Variability Responses to Intensified Training in Recreational Runners by Olli-Pekka Nuuttila, Heikki Kyröläinen, Veli-Pekka Kokkonen, Arja Uusitalo

“…Abstract Background Resting heart rate (HR) and HR variability (HRV) are widely used parameters to assess cardiac autonomic nervous system function noninvasively. While resting assessments can be performed during sleep or after awakening, it would be relevant to know how interchangeable the results of these measurements are. …”
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Effects of sinomenine on PI3K/mTOR signaling pathway and mesangial hyperplasia in rats with adriamycin-induced nephropathy by Liu Yue, Li Peng-yuan, Zhang Qing-hua, Yan Dan

“…Objective To explore the effect of sinomenine on the signal pathway of phosphatidyl-3 kinase（PI3 K）/mammalian target of rapamycin（mTOR）and mesangial hyperplasia in renal tissue of rats with adriamycin-induced nephropathy and elucidate its protective mechanism.Methods A total of 50 SD rats were selected, 10 rats were randomly injected with normal saline via caudal vein as control group, the other 40 rats were injected with adriamycin 5 mg/kg via caudal vein for 7 days to prepare nephrotic syndrome（NS）model.After successful modeling, the rats were randomly divided into the groups of model（NS）,sinomenine low-dose（25 mg/kg）,medium-dose（50 mg/kg）and high-dose（100 mg/kg）（n=10 each）.Control and NS groups received normal saline through abdominal cavity and sinomenine groups were given corresponding doses of drugs once daily for 28 days.At 24 hours after the last administration, blood and urine samples of rats in each group were collected and rats sacrificed, renal tissue samples harvested, serum creatinine（Scr）and urea nitrogen（BUN）were measured by enzyme-linked immunosorbent assay（ELISA）;24-hour urine protein was detected by Coomassie brilliant blue staining; hematoxylin-eosin staining（HE）was utilized for analyzing the pathological changes of kidney and mesangial matrix index（M/G）;transmission electron microscopy for observing the thickening of glomerular basement membrane of kidney; Western blot for detecting the expressions of p-PI3 K/PI3 K,p-mTOR/mTOR,fibronectin（FN）and type IV collagen（COL4）in renal tissue.Results As compared with control group, there were pathological damages such as the increase of glomerular mesangial matrix, the expansion of renal tubule lumen and severe thickening of glomerular basement membrane.M/G and renal function indicators Scr, BUN,24-hour urine protein and renal tissue were elevated.The protein expressions of p-PI3 K,p-mTOR,FN and COL4 rose markedly in NS group（P<0.05）.As compared with NS group, sinomenine high/medium/low dose groups alleviated pathological damage such as glomerular basement membrane thickening, M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,expressions of mTOR,FN and COL4 proteins declined markedly in sinomenine low/medium/high-dose groups（P<0.05）.As compared with low-dose sinomenine group, glomerular basement membrane thickening was the least pathologically damaged and M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,p-mTOR,FN and COL4 declined markedly in sinomenine medium/high-dose group（P<0.05）.Conclusions Sinomenine may inhibit the activation of PI3 K/mTOR signaling pathway, reduce the proliferation of glomerular mesangial cells and delay the process of nephrotic syndrome.…”
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