A non-hypothesis-driven practical laboratory activity on functional metagenomics: “fishing” protein-coding DNA sequences from microbiomes by Melissa Morra, Denise Marradi, Luca Gandini, Vittorio Ivagnes, Giulia Ottolini, Alessandro Bovio, Grace Jabali, Lorenzo Maraschi, Ifeoluwa Ayomide Dada, Tonderai Vitalis Chawanda, Martina Gorla, Olga Tarasiuk, Chiara Mocchetti, Maria Felicia Soluri, Maria Felicia Soluri, Francesca Boccafoschi, Daniele Sblattero, Diego Cotella

“…Here, we describe a simple protocol for the construction of a metagenomic DNA library, optimized and tested by a team of undergraduate biotechnology students. …”
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Wild ActionFormer: Enhancing wildlife action recognition for 11 endangered species in Wolong by Dan Liu, Qianqian Sun, Jin Hou, Bochuan Zheng, Jindong Zhang, Desheng Li, Tomas Norton, Jifeng Ning

“…We utilize UniformerV2 as the base backbone network, integrating self-supervised learning methods to enhance feature extraction capabilities. …”
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Design and simulation of a 5 KW solar-powered hybrid electric vehicle charging station with a ANN–Kalman filter MPPT and MPC-based inverter control for reduced THD by Youness Hakam, Hajar Ahessab, Ahmed Gaga, Mohamed Tabaa, Benachir El Hadadi

“…This ensures a reliable and sustainable power source for EV charging, reducing grid dependency during peak demand. …”
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Analisis Sistim Manajemen Dokumentasi Keperawatan pada Poliklinik Gigi Rumah Sakit di Bukittinggi by Dewi Rosmalia, Rizanda Machmud, Haryadi Mangkuto

“…The study was conducted with qualitative analysis methods, data sources derived from observations, documents and in-depth interviews. …”
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Freeform Optics for Achieving Collimated and Uniform Light Distribution in LCD-Type UV-Curable 3D Printing by Zhengbo Zhu

“…Initially, a ray mapping between the source domain and the target domain is established to design the preliminary freeform lens, assuming a point-like light source. …”
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Numerical analysis on heat and mass transfer of immiscible fluids in a vertical channel with diffusion effects by P. Kumaraswamy, T Ramakrishna Goud, B. Suresh Babu, Vanaja Gosty, G. Srinivas, T. Haripriya, O.D. Makinde

“…The study further incorporates the effects of an internal heat source, adding complexity to the analysis of transfer processes. …”
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Future Circular Lepton Collider Vibrational Crosstalk by Purinut Lersnimitthum, Audrey Piccini, Federico Carra, Tirawat Boonyatee, Niphon Wansophark, Nopdanai Ajavakom

“…It was found that the overall gain of crosstalk was less than 10% of that of ground-to-magnetic axis of either the Collider or Booster. This method may be used to optimize the tunnel layout from a vibration point of view. …”
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Leveraging Large Language Models for Predicting Postoperative Acute Kidney Injury in Elderly Patients by Hanfei Zhu, Ruojiang Wang, Jiajie Qian, Yuhao Wu, Zhuqing Jin, Xishen Shan, Fuhai Ji, Zixuan Yuan, Tingrui Pan

“…LLMs, with extensive pretraining and text generation capabilities, offer a new solution. Methods: We applied prompt engineering and knowledge distillation based on instruction fine-tuning to optimize LLMs for AKI prediction. …”
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Baby Blues Syndrome in the Qur'an: A Resilience Study of Maryam's Story by Novita Safitri, Ris'an Rusli, Apriyanti Apriyanti, Uswatun Hasanah, Kusnadi Kusnadi

“…Primary data sources were gathered from Qur'anic verses, while secondary sources included tafsir (Qur'anic exegesis) books, scientific journals, and articles related to mental health. …”
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Development and Validation of a Suite of E-Probes for Electronic Diagnostic Nucleic Acid Analysis (EDNA) for 20 Graft-Transmissible Pathogens of Citrus Using MiFi and Blind Ring Te... by D. M. Nascimento, S. Bodaghi, H. Wang, M. R. Ribeiro-Junior, R. Campos, T. Dang, F. Osman, J. Habiger, A. S. Espindola, G. Vidalakis, K. F. Cardwell

“…These e-probes were optimized using the MiFi bioinformatics platform for specificity, sensitivity, and detection limits through both in silico and in planta testing. …”
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Biological characterization and in vitro fungicide screening of a new pathogen of basal stem rot of Schisandra chinensis in Jilin Province, China by Taiping Tian, Mengqi Wang, Jingmeng Zhu, Yining Sun, Mingjie Ma, Guoliang Liu, Jinshuo Wang, Bing Zhao, Yiping Yan, Yue Wang, Yue Wang, Yanli Wang, Yanli Wang, Changyu Li, Changyu Li, Dongyin Wang, Fang Yang, Wenpeng Lu, Wenpeng Lu, Peilei Xu, Peilei Xu

“…Optimal growth conditions included CDA or CMA media, 25°C, continuous darkness, and pH 7. …”
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Test accuracy of loop-mediated isothermal amplification for schistosomiasis in low endemicity areas: a systematic review and meta-analysis by Xinjie Zhou, Jiajia Li, Jiayin Qiu, Ting Feng, Chao Lv, Wangping Deng, Robert Bergquist, Jing Xu, Shizhu Li, Zhiqiang Qin

“…Subgroup analyses and univariable meta-regression were performed to explore the source of heterogeneity. Specifically, subgroup analyses were performed by categorizing into species (S. japonicum, S. mansoni, S. haematobium), sample type (stool, urine, serum, snails), and DNA extraction methods to explore factors influencing test performance. …”
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FEN-MRMGCN: A Frontend-Enhanced Network Based on Multi-Relational Modeling GCN for Bus Arrival Time Prediction by Ting Qiu, Chan-Tong Lam, Bowie Liu, Benjamin K. Ng, Xiaochen Yuan, Sio Kei Im

“…Accurate bus arrival time prediction is crucial for enhancing passenger experience and optimizing smart city transit systems. Existing methods, typically based on single-route, sparse stop data, struggle with the complex spatiotemporal interactions present in dense stop areas and multi-route networks, resulting in lower prediction accuracy. …”
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Hybrid causal inference framework for long-chain drought propagation networks: Combining convergent cross-mapping and generalized random forest by Jiayou Wang, Zhi Li, Yaning Chen, Shiru Han, Lingxin Kong

“…The hydrological cycle in inland river basins of arid regions operates as a closed system with diverse recharge sources. In these areas, drought propagation exhibits strong high-dimensional nonlinearity, making it difficult for traditional linear models and time–frequency analysis methods to capture the complex dynamic causal relationships. …”
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Effects of Continuous Straw Returning Combined with Reduced Fertilizer Application on Rice Yield and Loss of Nitrogen and Phosphorus by Yao Li, Wang Hong, Zhang Qi, Liu Haitao, Luo Fuxiang, Wang Xie, Zheng Shenghua, Liang Fan, Lin Chaowen

“…[Objective] The effects of straw returning combined with reduced chemical fertilizer application on rice yield and nitrogen and phosphorus losses with runoff were studied in order to provide a scientific basis for the recycling and reuse of local agricultural resources and the prevention and control of agricultural non-point source pollution. [Methods] A three-year (2018—2020) field plot experiment was conducted that included two treatments: conventional fertilization (T1), and straw returning + conventional fertilization reduced by 28.57% for nitrogen and 25.11% for phosphorous (T2). …”
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Origin Traceability of Chinese Mitten Crab (<i>Eriocheir sinensis</i>) Using Multi-Stable Isotopes and Explainable Machine Learning by Danhe Wang, Chunxia Yao, Yangyang Lu, Di Huang, Yameng Li, Xugan Wu, Weiguo Song, Qinxiong Rao

“…The Chinese mitten crab (<i>Eriocheir sinensis</i>) industry is currently facing the challenges of origin fraud, as well as a lack of precision and interpretability of existing traceability methods. Here, we propose a high-precision origin traceability method based on a combination of stable isotope analysis and interpretable machine learning. …”
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Precise Design of Solid Rocket Motor Heat Insulation Layer Thickness under Nonuniform Dynamic Burning Rate by Ran Wei, Futing Bao, Yang Liu, Weihua Hui

“…With the purpose of obtaining optimal designs of the heat insulating layers in solid rocket motors, we have proposed a numerical approach to compute the ideal thickness of the heat insulating layer. …”
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PEDAGOGICAL VALUE OF THE COMMUNICATION AS A PROFESSIONAL ETHICAL PHENOMENON IN THE EDUCATION OF DENTISTS STUDENTS by R.M. Khopta

“…The doctor must owns such methods as a persuasion and a suggestion in his daily professional activities and be able to establish emotional or business contact in communication with patients and colleagues. …”
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The efficacy of low-level laser therapy in oral surgery: A systematic review of randomized controlled trials by Alain Chaple Gil, Leonardo Díaz, Alfredo Von Marttens, Claudio Sotomayor, Javier Basualdo, Víctor Beltrán, Gilbert Jorquera, Rodrigo Caviedes, Eduardo Fernández

“…It also aimed to identify optimal laser parameters for future clinical application. …”
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From digitized whole‐slide histology images to biomarker discovery: A protocol for handcrafted feature analysis in brain cancer pathology by Xuanjun Lu, Yawen Ying, Jing Chen, Zhiyang Chen, Yuxin Wu, Prateek Prasanna, Xin Chen, Mingli Jing, Zaiyi Liu, Cheng Lu

“…Among the 72 models combining nine feature selection methods and eight machine learning classifiers, the optimal model combination achieved discriminative performance with an average area under the curve (AUC) of 0.615 over 100 iterations under five‐fold cross‐validation. …”
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