The concentration of single-stranded DNA-binding proteins is a critical factor in recombinase polymerase amplification (RPA), as revealed by insights from an open-source system by Francisco Cordoba-Andrade, Antolin Peralta-Castro, Paola L. García-Medel, Eduardo Castro-Torres, Rogelio Gonzalez-Gonzalez, Atzimba Y. Castro-Lara, Josue D. Mora Garduño, Claudia D. Raygoza, Noe Baruch-Torres, Alejandro Peñafiel-Ayala, Corina Diaz-Quezada, Cesar S. Cardona-Felix, Fernando Guzman Chavez, Carlos H. Trasviña-Arenas, Rogerio R. Sotelo-Mundo, Beatriz Xoconostle-Cazares, Agustino Martínez-Antonio, Luis Gabriel Brieba de Castro

“…In this study, we introduce an open-source alternative to commercial RPA kits, utilizing purified, heterologously expressed proteins to circumvent the fixed molar ratios of proprietary systems. …”
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Impact of arm position on low dose dual-source CT one-step aortic and cerebral-carotid artery angiography image quality and radiation dose by Meng Wang, Chao Zheng, Lin Yang, Juan Su, Jiexin Sheng, Xiaolong He, Bo Wang, GuangHang Wu

“…Methods Between January 2022 and August 2023,a total of 185 patients were enrolled in the study and underwent low-dose one-stop cerebral carotid aortic CTA. …”
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Benchmark dataset and deep learning method for global tropical cyclone forecasting by Cheng Huang, Pan Mu, Jinglin Zhang, Sixian Chan, Shiqi Zhang, Hanting Yan, Shengyong Chen, Cong Bai

“…Comprehensive evaluations demonstrate that TCN M outperforms both existing deep learning methods and official meteorological forecasts across multiple metrics. …”
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Research on Multi-Machine Pre-Synchronization Control and Optimization Based on Parallel Recovery Black Start by Zhongping Ruan, Shuye Ding, Yizhi Chen

“…Simulation results show that the proposed method suppresses frequency overshoot and system disturbances during a multi-source black start, significantly enhancing microgrid restoration capability and operational stability.…”
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Heat Storage Decoupling Method Based on Coordinated Load Scheduling Model by Yi LEI, Mingzhen LIU, Kaimin LIN

“…The controllable heat storage electric boilers are used in this paper as peak regulation and dispatch resources, in order to reduce the forced output of CHP and get a lower load peak and a higher load valley when it's hard to regulate the peak load, and to increase the wind power accommodation capacity of power grid in winter, and to form a load-dispatching model featuring source-load coordination. The power-heat coupling characteristics of CHP units are firstly studied; and then a heat-power decoupling scheme is formulated for wind power-heat storage compensation, and a multi-objective load-dispatching model is built with the objective of minimizing both the operation cost and pollutant emission of the power system; finally, the corrected multi-objective particle swarm optimization algorithm is used to solve the model, and the diversity of the Pareto set is maintained by using niche method. …”
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Brain tumor detection through image fusion using whale optimization and edge preserving filter by Bhattiprolu Nagasirisha, Venkatesh Munagala, Ramakrishna Reddy Eamani, Mocherla Venkata Srikanth, Asileti Suneel Kumar, Varre Venkata Kanaka Durga Prasad

“…While the detail layers are fused based on maximum energy assessed using the Krisch compass operator, the base layers are fused based on the whale optimization method,  for which the objective function is maximization of entropy, edge strength and pixel intensity. …”
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Extracting Organic Compounds from Brewer's Spent Grain by Various Methods by Irina N. Gribkova, Larisa N. Kharlamova, Elena M. Sevostianova, Irina V. Lazareva, Maxim A. Zakharov, Olga A. Borisenko

“…This article focuses on innovative extraction methods, including sustainable deep processing that destroys the internal structures of plant matrix. …”
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Optimal Anisotropic Guided Filtering in retinal fundus imaging: A dual approach to enhancement and segmentation. by G Tirumala Vasu, Samreen Fiza, Subba Rao Polamuri, K Reddy Madhavi, Thejaswini R, Venkataramana Guntreddi

“…In the second stage, optimized top-hat transform and homomorphic filtering has been performed to get segmented image. …”
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Research progress of main microflora and their roles during strong-flavor Baijiu Daqu fermentation by HE Xiaorong, ZHANG Qing, TANG Jiahuan, LIU Pei

“…In this paper, the main microbial sources in the fermentation process of strong-flavor (Nongxiangxing) Baijiu Daqu, the changes of microorganisms in different fermentation stages, the role of important functional microorganisms, and the methods of microbial community analysis were reviewed, in order to provide scientific basis for optimization of strong-flavor Daqu production process and improvement of Baijiu quality.…”
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Audio signal transmission method in network-based audio analytics system by Anton Poroshenko, Andriy Kovalenko

“… The subject matter of the article is аudio signal transmission method in network-based audio analytics system. The creation of a network-based audio analytics system leads to the emergence of new classes of load sources that transmit packetized sound data. …”
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Audio signal transmission method in network-based audio analytics system by Антон Порошенко, Андрій Коваленко

“… The subject matter of the article is аudio signal transmission method in network-based audio analytics system. The creation of a network-based audio analytics system leads to the emergence of new classes of load sources that transmit packetized sound data. …”
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Research on influencing factors and application of phosphorus recovery by induced crystallization method by QI Zhen, LIU Rupeng, XU Linxu, FANG Jinfeng, ZHU Weichen, FAN Haoyu, QIAO Yimu

“…Finally, the development prospects and optimization of process application conditions for the recovery and utilization of phosphorus resources by induced crystallization method were discussed.…”
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Dissolved Oxygen Prediction Based on SOA-SVM and SOA-BP Models by ZHANG Xuekun

“…To improve the accuracy of dissolved oxygen prediction,this paper researches and proposes a prediction method that combines seagull optimization algorithm (SOA) with support vector machine (SVM) and BP neural network,prepares four prediction schemes based on the monthly dissolved oxygen monitoring data of the Jinghong Power Station in Xishuangbanna,a national important water supply source in Yunnan Province,from January 2009 to September 2020,optimizes the key parameters of SVM and weight threshold of BP neural network by SOA to construct SOA-SVM and SOA-BP models,predicts the dissolved oxygen of Jinghong Power Station based on the models,and compares the prediction results with those of SVM and BP models.The results show that:The absolute values of the average relative errors of the SOA-SVM and SOA-BP models for the 4 schemes of dissolved oxygen prediction are between 4.07%~4.98% and 3.85%~4.83%,and that of the average absolute errors are 0.309~0.374 mg/L and 0.294~0.371 mg/L,respectively.With better prediction accuracy than SVM and BP models,they have good prediction accuracy and generalization ability.SOA can effectively optimize the key parameters of SVM and weight threshold of BP neural network.SOA-SVM and SOA-BP models are feasible for dissolved oxygen prediction,which can provide references for related prediction research.…”
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Optimizing breeding strategies for early-maturing white maize through genetic diversity and population structure. by Hellen Mawia Mukiti, Baffour Badu-Apraku, Ayodeji Abe, Idris Ishola Adejumobi, John Derera

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Optimizing Solar Energy Harvesting: A K‐Means Clustering Approach for Enhanced Efficiency and Viability by Li Bin, Muhammad Shahzad, Muhammad Farhan, Muhammad Sanaullah Khan, Mubaarak Abdulrahman Abdu Saif, Girmaw Teshager Bitew

“…It has emerged as a primary source; however, optimizing energy utilization and solar panel efficiency to maximize absorbed solar radiation remains a significant challenge. …”
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Proton Exchange Membrane Fuel Cells: An Effective Neural Fuzzy System for Optimal Power Tracking by Assala Bouguerra, Abd Essalam Badoud, Saad Mekheilef

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Installation of an Electric Car Charger Using the Bald Eagle Optimizer Cascaded PI and LQR Controller by Ali Huzbur Hussien, Khalid Mohammed Ameen Alazawi

“…The Golden Eagle optimization methods are employed to enhance the setting of Proportional-Integral (PI) and Linear Quadratic Regulator (LQR) controller settings for improved conversion efficiency. …”
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A diffractometer project for Brazilian Multipurpose Reactor (RMB): McStas simulations and instrument optimization by Alexandre Pinho dos Santos Souza, Luiz Paulo de Oliveira, Fabiano Yokaichiya, Frederico Antonio Genezini, Margareth Kazuyo Kobayashi Dias Franco

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A Systematic Review and Evolutionary Analysis of the Optimization Techniques and Software Tools in Hybrid Microgrid Systems by Kawakib Arar Tahir

“…This study identifies key research trends, highlights gaps in the optimization strategies, and emphasizes the need for AI integration, broader adoption of open-source tools, and scalable optimization frameworks. …”
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Construction and optimization of health behavior prediction model for the older adult in smart older adult care by Qian Guo, Peiyuan Chen

“…Future improvements, integrating more data and optimizing technology, will strengthen its potential for providing comprehensive support in older adult care services.…”
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