A practical distribution pattern of α-SMA-positive carcinoma associated fibroblasts indicates poor prognosis of patients with pancreatic ductal adenocarcinoma by Bo Li, Meilong Shi, Yang Wang, Penghao Li, Xiaoyi Yin, Guoxiao Zhang, Xiaochao Kang, Huan Wang, Suizhi Gao, Kailian Zheng, Xiaohan Shi, Xiongfei Xu, Yukun Zhou, Hui Jiang, Wei Jing, Shiwei Guo, Gang Jin

“…We utilized a tissue microarray to assess the spatial intensity of α-SMA expression within the tumor microenvironment. …”
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SVseq discloses the genomic complexity of different prenatal, de novo, apparently balanced chromosome rearrangements detected by CMA and karyotype by Shengfang Qin, Xueyan Wang, Chun Chen, Jin Wang, Zhuo Zhang, Yan Yin, Xiangyou Leng

“…Methods Five children with BCRs detected through karyotyping and chromosome microarray analysis (CMA) during prenatal diagnoses were analyzed using SVseq technology. …”
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Identification of Tumor‐Specific Surface Proteins Enables Quantification of Extracellular Vesicle Subtypes for Early Detection of Pancreatic Ductal Adenocarcinoma by Chen Zhao, Zhili Wang, Hyoyong Kim, Hui Kong, Junseok Lee, Jacqueline Ziqian Yang, Anmin Wang, Ryan Y. Zhang, Yong Ju, Jina Kim, Bing Feng, Dejun Liu, Yating Zhang, Zhenfang Wang, Yandong Zhang, Shujing Guo, Dekang Gao, James S. Tomlinson, Renjun Pei, Jipeng Wan, Stephen J. Pandol, Myung‐Shin Sim, Sungyong You, Ding Ma, Shaohua Lu, Na Sun, Hsian‐Rong Tseng, Yazhen Zhu

“…Abstract Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer‐related mortality, largely due to late‐stage diagnosis. Reliable early detection methods are critically needed. PDAC‐derived extracellular vesicles (EVs) carry molecules that reflect their parental tumor cells and are detectable in early disease stages, offering a promising noninvasive diagnostic approach. …”
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Tumour extracellular vesicle surface Protein-mRNA integration assay for early detection of epithelial ovarian cancerResearch in context by Ying-Tzu Yen, Chen Zhao, Tian Gao, Jacqueline Ziqian Yang, Ning Kang, Jiahui Pu, Lei Qiu, Qixin Hu, Hyoyong Kim, Anmin Wang, Junseok Lee, Ryan Y. Zhang, Na Liu, Yue Ma, You-Ren Ji, Yong Ju, Lynn L. Zheng, James Lee-South, Vivian X. Zuo, Audrey Qian, Aaron Kwan, Yating Zhang, Shenghua Zhang, Zhili Wang, Jing Ren, Huaichao Liu, Zihan Wang, Yang Yue, Jina Kim, Jennifer Sun, Gabriella A. DiBernardo, Laura B. James-Allan, Ying Chen, Weipei Zhu, Guoyun Wang, Renjun Pei, Sanaz Memarzadeh, Sungyong You, Bobbie J. Rimel, Kate Lawrenson, Beth Y. Karlan, Myung Shin Sim, Shaohua Lu, Jipeng Wan, Na Sun, Hsian-Rong Tseng, Yazhen Zhu

“…Three EOC EV surface protein markers (i.e., FRα, MSLN, and TROP2) were selected through a bioinformatic framework using multi-omics data and underwent rigorous validation using EOC cell lines and EOC tissue microarrays. …”
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Rapid Detection of Panton–Valentine Leukocidin Production in Clinical Isolates of <i>Staphylococcus aureus</i> from Saxony and Brandenburg and Their Molecular Characterisation by Elke Müller, Stefan Monecke, Marc Armengol Porta, Marco Vinicio Narvaez Encalada, Annett Reissig, Lukas Rüttiger, Percy Schröttner, Ilona Schwede, Hans-Herman Söffing, Alexander Thürmer, Ralf Ehricht

“…They were also characterized using DNA microarrays, facilitating the detection of virulence and resistance markers as well as the assignment to clonal complexes and epidemic/pandemic strains. …”
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Exposing Optimal Feature Sets for Enhancing Machine Learning Performance by Hiba Mohammed Al-Marwai, Ghaleb H. Al-Gaphari, Mohammed Mohammed Zayed

“…To evaluate the effectiveness of our approach, we conduct experiments on benchmark microarray datasets from the ADNI database. Comparative analysis is performed against six traditional single objective methods and five other existing multiobjective methods. …”
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Successful strategy of comprehensive pre-implantation genetic testing for Duchenne muscular dystrophy and chromosome balance using karyomapping and fluorescent PCR by Suchada Mongkolchaipak, Sirivipa Piyamongkol, Chutithep Teekaput, Rungthiwa Sirapat, Wanwisa Suriya, Tawiwan Pantasri, Theera Tongsong, Wirawit Piyamongkol

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AssayBLAST: A Bioinformatic Tool for <i>In Silico</i> Analysis of Molecular Multiparameter Assays by Maximilian Collatz, Sascha D. Braun, Martin Reinicke, Elke Müller, Stefan Monecke, Ralf Ehricht

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B7-H3 and B7-H4 expression in phyllodes tumors of the breast detected by RNA in situ hybridization and immunohistochemistry: Association with clinicopathological features and T-cel... by Ga-Eon Kim, Nah Ihm Kim, Min Ho Park, Ji Shin Lee

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The Use of Multiplex Phosphorescence Analysis (PHOSPHAN^TM) and Polymerase Chain Reaction for Laboratory Diagnosis of Ixodid Tick-borne Borrelioses by T. I. Kuznetsova, V. G. Pomelova, E. I. Korenberg, N. S. Osin

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Implementation of Valid HPV Diagnostics for the Early Detection of Cervical Cancer in Molecular Pathology: HPV 3.5 LCD-Array (Chipron GmbH) vs. PapilloCheck^® (Greiner Bi... by Jan Jeroch, Melanie Winter, Anna Bieber, Agnes Boger, Christina Schmitt, Silvana Ebner, Morva Tahmasbi Rad, Henning Reis, Peter. J. Wild

“…This study compares the two hybridisation-based DNA-microarray systems “HPV 3.5 LCD-Array” (Chipron GmbH) and “PapilloCheck^®” (Greiner Bio-One GmbH), based on their ability to detect and differentiate HPV infections in 42 different cervical smears. …”
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The Features of a Humoral Immune Response Development during the Acute Phase of ixodes Tick-Borne Borreliosis (Based on Multiplex Analysis with the Immunochip) by V. G. Pomelova, E. I. Korenberg, T. I. Kuznetsova, N. S. Osin

“…Therefore, additional detection of IgM to recombinant proteins did not improve significantly the overall sensitivity of the latter PHOSPHAN variant. …”
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Evaluating the potential of anti-dsRNA antibodies as an alternative viral sensing tool in encephalitides of different species by Madeleine de le Roi, Hannah Gerhards, Adnan Fayyad, Adnan Fayyad, Mathias Boelke, Stefanie Christine Becker, Asisa Volz, Ingo Gerhauser, Wolfgang Baumgärtner, Christina Puff

“…Molecular methods like multiplex PCR and microarrays are considered to be often less sensitive than Next Generation Sequencing, whereas the latter is time-consuming and costly. …”
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A RNA-Seq Analysis of the Rat Supraoptic Nucleus Transcriptome: Effects of Salt Loading on Gene Expression. by Kory R Johnson, C C T Hindmarch, Yasmmyn D Salinas, YiJun Shi, Michael Greenwood, See Ziau Hoe, David Murphy, Harold Gainer

“…In addition, we compare the SON transcriptomes resolved by RNA-Seq methods with the SON transcriptomes determined by Affymetrix microarray methods in rats under the same osmotic conditions, and find that there are 6,466 genes present in the SON that are represented in both data sets, although 1,040 of the expressed genes were found only in the microarray data, and 2,762 of the expressed genes are selectively found in the RNA-Seq data and not the microarray data. …”
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Mutation Spectrum of the ABCA4 Gene in a Greek Cohort with Stargardt Disease: Identification of Novel Mutations and Evidence of Three Prevalent Mutated Alleles by Kamakari Smaragda, Kokkinou Vassiliki, Koutsodontis George, Stamatiou Polixeni, Giatzakis Christoforos, Anastasakis Anastasios, Aslanides Ioannis Minas, Koukoula Stavrenia, Panagiotoglou Theoni, Datseris Ioannis, Tsilimbaris K. Miltiadis

“…Direct sequencing improved significantly the complete characterization rate, that is, identification of two mutations compared to the microarray analysis (93.1% versus 50%). In total, 40 distinct potentially disease-causing variants of the ABCA4 gene were detected, including six previously unreported potentially pathogenic variants. …”
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Genome-wide analysis of m6A-modified circRNAs in the mouse model of myocardial injury induced by obstructive sleep apnea by Jiuhuang Lan, Yuhui Wang, Chang Liu, Hongli Chen, Qingshi Chen

“…The results of MeRIP-qPCR were consistent with the microarray results. 73 pathways were detected in the up-regulated transcripts and no relevant pathways were detected in the down-regulated transcripts. …”
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Multiple colonization with S. pneumoniae before and after introduction of the seven-valent conjugated pneumococcal polysaccharide vaccine. by Silvio D Brugger, Pascal Frey, Suzanne Aebi, Jason Hinds, Kathrin Mühlemann

“…Serotypes were identified by agglutination, multiplex PCR and microarray.<h4>Principal findings</h4>Rate of multiple colonization remained stable up to three years after PCV7 introduction. …”
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R-locus for roaned coat is associated with a tandem duplication in an intronic region of USH2A in dogs and also contributes to Dalmatian spotting. by Takeshi Kawakami, Meghan K Jensen, Andrea Slavney, Petra E Deane, Ausra Milano, Vandana Raghavan, Brett Ford, Erin T Chu, Aaron J Sams, Adam R Boyko

“…The tandem duplication is in an intronic region of usherin gene (USH2A), which was perfectly associated with roaning but absent in non-roaned dogs. We detected strong selection signals in this region characterized by reduced nucleotide diversity (π), increased runs of homozygosity, and extended haplotype homozygosity in Wirehaired Pointing Griffons and Australian Cattle Dogs (typically roaned breeds), as well as elevated genetic difference (FST) between Wirehaired Pointing Griffon (roaned) and Labrador Retriever (non-roaned). …”
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High prevalence of multidrug-tolerant bacteria and associated antimicrobial resistance genes isolated from ornamental fish and their carriage water. by David W Verner-Jeffreys, Timothy J Welch, Tamar Schwarz, Michelle J Pond, Martin J Woodward, Sarah J Haig, Georgina S E Rimmer, Edward Roberts, Victoria Morrison, Craig Baker-Austin

“…Representative isolates were also examined for the presence of 54 resistance genes by a combination of miniaturized microarray and conventional PCR. Forty-seven of 94 Aeromonas spp. isolates recovered from tropical ornamental fish and their carriage water were tolerant to > or =15 antibiotics, representing seven or more different classes of antimicrobial. …”
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Global gene expression profiling reveals SPINK1 as a potential hepatocellular carcinoma marker. by Aileen Marshall, Margus Lukk, Claudia Kutter, Susan Davies, Graeme Alexander, Duncan T Odom

“…Six genes identified by microarray as highly expressed in HH-HCC were confirmed by RT qPCR. …”
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