Potential for Duplexed, In-Tandem gRNA-Mediated Suppression of Two Essential Genes of Tomato Leaf Curl New Delhi Virus in Crop Plants by Saher Naveed, Judith K. Brown, Muhammad Mubin, Nazir Javed, Muhammad Shah Nawaz-ul-Rehman

“…In this study, a guide RNA (gRNA) sequence-CRISPR-Cas9 approach was used to target and cleave two essential coding regions in the begomovirus genome. …”
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An Integrative Computational Approach for Identifying Cotton Host Plant MicroRNAs with Potential to Abate CLCuKoV-Bur Infection by Muhammad Aleem Ashraf, Imran Shahid, Judith K. Brown, Naitong Yu

“…RNA interference (RNAi) is a naturally occurring process that has revolutionized the targeting of gene regulation in eukaryotic organisms to combat virus infection. …”
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The interplay between RNA m6A modification and radiation biology of cancerous and non-cancerous tissues: a narrative review by Yajia Cheng, Yue Shang, Shuqin Zhang, Saijun Fan

“…N6-methyladenosine (m6A), a prevalent modification on coding and non-coding RNAs, is a key epigenetic mark established by a set of evolutionarily conserved enzymes. …”
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Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system [version 1; peer review: 2 approved] by Aimée Finlayson, Domenico Modaffari, Edward W. J. Wallace, Yuyang Miao, Kenneth E. Sawin

“…We demonstrate precise, scarless genome editing with nucleotide-level DNA substitutions, and we demonstrate markerless gene tagging by fusing fluorescent-protein coding sequences to the endogenous coding sequences of several A. nidulans genes. …”
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Circ-PRMT5 promotes gastric cancer progression by sponging miR-145 and miR-1304 to upregulate MYC by Wenbo Du, Ding Li, Xiaomin Guo, Ping Li, Xin Li, Shuping Tong, Junhui Tong, Lihua Kuang, Daoming Liang

“…Circular RNAs (circRNAs) are a new type of non-coding RNA and promising biomarkers for diagnosis of multiple diseases such as cancer.Methods Circ-PRMT5 expression was validated in 90 GC patient tissues and 6 different GC cells by qRT-PCR. …”
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LncRNA GABARAPL2 promotes non‑union long bone fractures by suppressing bone mesenchymal stromal cell osteogenesis by Hurong Du, Wenlong Zhang, Zeyuan Liu, Wei Liu

“…Growing evidence suggests that non-coding RNAs play crucial regulatory roles in bone metabolism. …”
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Regulatory mechanisms and clinical implications of PIWI-interacting RNAs (piRNAs) in major digestive tract cancers by Penghui Li, Yuan Xue, Xinyu Gu

“…Furthermore, therapeutic interventions targeting dysregulated piRNAs or their downstream effectors hold promise as novel avenues for precision medicine approaches in managing digestive tract cancers. …”
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The pre-mRNA splicing modulator pladienolide B inhibits Cryptococcus neoformans germination and growth by Sierra L. Love, Megan C. McKeon, Henrik Vollmer, Joshua C. Paulson, Nanami Oshimura, Olivia Valentine, Sébastien C. Ortiz, Christina M. Hull, Aaron A. Hoskins

“…New treatments and therapeutic targets are needed to combat these pathogens. One potential target is the pre-mRNA processing pathway, which is required for expression of nearly all protein-coding genes in C. neoformans. …”
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Multimorbidity combinations, costs of hospital care and potentially preventable emergency admissions in England: A cohort study. by Jonathan Stokes, Bruce Guthrie, Stewart W Mercer, Nigel Rice, Matt Sutton

“…Key limitations include availability of data only from hospitals and reliance on hospital coding of health conditions.<h4>Conclusions</h4>Our findings indicate that there are no clear multimorbidity combinations for a cluster-targeted intervention approach to reduce secondary care costs. …”
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EXPLORING EMMA BOVARY’S DIFFUSED PERSONALITY IN GUSTAVE FLAUBERT’S MADAME BOVARY NOVEL by Zuvyati Aryani Tlonaen

“…The research procedure was reading the novel, identifying the characters' behavior, thoughts, words, and action, classifying sentences or phrases that show 12 diffusion features, coding, and verification. The study showed that Emma has the twelve diffused personality features. …”
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The general intelligence of GPT–4, its knowledge diffusive and societal influences, and its governance by Mohammad Mahdi Jahani Yekta

“…It exhibits capabilities in abstraction, comprehension, vision, computer coding, mathematics, and more, suggesting it to be a significant step towards artificial general intelligence (AGI), a level of AI that possesses capabilities similar to human intelligence. …”
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Transparency and reproducibility in invasion science by Fabio Mologni, Jason Pither

“…This includes explicitly considering and ideally estimating statistical power, undertaking a study pre-registration, making all relevant code and non-sensitive raw data accessible and useable, and devising and upholding clear and consistent policies on transparent reporting and open materials.…”
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Improving asthma care in children: revealing needs and bottlenecks through in-depth interviews by Casper E. W. Gijsen, Carolien van Rossem, Jean W. M. Muris, Marieke W. P. van Horck, Edward Dompeling

“…Moreover, children are often not actively involved in decision-making. The study provides valuable insights to improve the quality and continuity of care for children with asthma and their parents. …”
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Enhanced Curvature-Based Fabric Defect Detection: A Experimental Study with Gabor Transform and Deep Learning by Mehmet Erdogan, Mustafa Dogan

“…This method is particularly efficient due to its low data storage requirements and minimal processing time, making it ideal for real-time applications. Furthermore, we implemented and evaluated several other methods from the literature, including Gabor and Convolutional Neural Networks (CNNs), within a unified coding framework. …”
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DAWN: Designing Distributed Agents in a Worldwide Network by Zahra Aminiranjbar, Jianan Tang, Qiudan Wang, Shubha Pant, Mahesh Viswanathan

“…The rapid evolution of Large Language Models (LLMs) has transformed them from basic conversational tools into sophisticated entities capable of complex reasoning and decision-making. These advancements have led to the development of specialized LLM-based agents designed for diverse tasks such as coding and web browsing. …”
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Being An Wholistic and Developmental Healthcare Leader: Insights from a grounded theory research study by Shannon Richards-Green, Suzanne Gough, Mickan Sharon

“…Memo writing throughout the data collection and analysis process afforded additional valuable data. Results: Coding of data using constant comparative analysis rendered six key categories: Broadening perspectives and abilities as a leader, Creating the best possible healthcare environment, Experiencing and making sense of the bad times, Leading in alignment with personal values, Communicating and building relationships, Experiencing and making sense of the rewarding times. …”
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Analytic Hierarchy Process for an Automated Evaluation of Learning Difficulties of Novice Programmers by Salvatore Venticinque, Alba Amato, Pietro Fusco, Francesco Palmieri

“…The utilization of state-of-the-art AI techniques for decision-making in specific application domains is considered a high-risk practice, such as in Education. …”
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Psychometric Hepatic Encephalopathy Tests and Electroencephalogram Results Among Cirrhotic Patients by Alaa Aboud Mohamed, Mostafa M. Elkholy, Ola O. Mangoud, Ahmed R. N. Ibrahim, Marwa O. Elgendy, Ali M. Abdel Fattah

“…In the Letter Cancellation Test (LCT) (total error), 80% of patients had organic disorders, and 24% showed affections with (LCT) (completion time). The Digit Symbol Coding Test results showed affection in 28% of patients. …”
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Narrative Innovation In The Age Of Streaming: Algorithmic Storytelling And The Anthological Aesthetics Of Love, Death & Robots by Houssem Eddine MERZOUGUI

“…Drawing on a multi-methodological approach that includes close textual analysis and spreadsheet-assisted thematic coding, the study identifies key narrative strategies such as micro-narrative formatting, stylistic modularity, and audience-personalised aesthetics. …”
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Exploring PCAT3 Expression in Arthritis: Proinflammatory Marker Patterns and P53 Involvement by Aida H. Alobaidi, Entkhab M. A. Alanisi, Khalid A. Alyodawi

“…This study investigated the role of long non-coding RNA (lncRNA) PCAT3 in arthritis, focusing on its impact on proinflammatory markers. …”
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