Malate initiates a proton-sensing pathway essential for pH regulation of inflammation by Yu-jia-nan Chen, Rong-chen Shi, Yuan-cai Xiang, Li Fan, Hong Tang, Gang He, Mei Zhou, Xin-zhe Feng, Jin-dong Tan, Pan Huang, Xiao Ye, Kun Zhao, Wen-yu Fu, Liu-li Li, Xu-ting Bian, Huan Chen, Feng Wang, Teng Wang, Chen-ke Zhang, Bing-hua Zhou, Wan Chen, Tao-tao Liang, Jing-tong Lv, Xia Kang, You-xing Shi, Ellen Kim, Yin-hua Qin, Aubryanna Hettinghouse, Kai-di Wang, Xiang-li Zhao, Ming-yu Yang, Yu-zhen Tang, Hai-long Piao, Lin Guo, Chuan-ju Liu, Hong-ming Miao, Kang-lai Tang

“…Here, we found that L-malate exerts anti-inflammatory effect via BiP-IRF2BP2 signaling, which is a sensor of cytosolic pH in macrophages. …”
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Advanced strategies for screening and identifying RNA-targeted small molecules: Bridging therapeutic potential and innovation by Zukela Ruzi, Daxiong Han, Kailibinuer Aierken

“…In recent years, significant advancements in screening methodologies, such as high-throughput screening (HTS), small molecule microarrays (SMMs), and DNA-encoded libraries (DELs), have facilitated the identification of RNA-binding small molecules with enhanced specificity. …”
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Thrombospondin-1 induces CD8+ T cell exhaustion and immune suppression within the tumor microenvironment of ovarian cancer by Haiyan Liang, Suwei Zhang

“…Methods We analyzed THBS1 expression and its clinical significance using publicly available datasets (TCGA-OV, GSE14407) and tissue microarrays containing OC and adjacent normal tissues. …”
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Increased COX-2 Immunostaining in Urothelial Carcinoma of the Urinary Bladder Is Associated with Invasiveness and Poor Prognosis by Basim Al-Maghrabi, Wafaey Gomaa, Mohammed Abdelwahed, Jaudah Al-Maghrabi

“…UCB samples were collected before any local or systemic therapy. Tissue microarrays were designed and constructed, and TMA blocks were sliced for further immunohistochemical staining. …”
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SUMOYLATION AS AN EARLY ADAPTIVE MECHANISM IN THE PROTEOMIC RESPONSE OF HUMAN GERM CELLS TO SIMULATED MICROGRAVITY

“…This study analyses the effects of simulated microgravity (SμG) and hypogravity (ShG) on different pathways including SUMOylation (Small Ubiquitin-like Modifier) in human male germ cell line TCam-2, combining Reverse-Phase Protein microArrays (RPPA) and immunofluorescence. …”
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Prenatal tobacco smoke exposure is associated with childhood DNA CpG methylation. by Carrie V Breton, Kimberly D Siegmund, Bonnie R Joubert, Xinhui Wang, Weiliang Qui, Vincent Carey, Wenche Nystad, Siri E Håberg, Carole Ober, Dan Nicolae, Kathleen C Barnes, Fernando Martinez, Andy Liu, Robert Lemanske, Robert Strunk, Scott Weiss, Stephanie London, Frank Gilliland, Benjamin Raby, Asthma BRIDGE consortium

“…Some of the detrimental effects may be due to epigenetic modifications, although few studies have investigated this hypothesis in detail.…”
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Expression patterns of MCM8 in lung adenocarcinoma and its correlation with key biological processes by Xu-Sheng Liu, Jin Xie, Rui-Min Wu, Gao-Chun Xiao, Yu Zhang, Zhi-Jun Pei

“…The mechanism underlying the effect of MCM8 on cancer development and the immune response remains to be elucidated.…”
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DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients by Binbin Han, Jiwen Wang, Jia Gao, Shana Feng, Yu Zhu, Xuexiang Li, Ting Xiao, Jun Qi, Wei Cui

“…DJ-1 appears to have its greatest effect on tumourigenesis, and it may have a greater impact on early-stage lung cancers. …”
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Whole stromal fibroblast signature is linked to specific chemokine and immune infiltration patterns and to improved survival in NSCLC by Stefan Koeck, Arno Amann, Johan Kern, Marit Zwierzina, Edith Lorenz, Sieghart Sopper, Heinz Zwierzina, Finn Mildner, Martina Sykora, Susanne Sprung, Hubert Hackl, Florian Augustin, Hubert T. Maier, Andreas Pircher, Georg Pall, Dominik Wolf, Gabriele Gamerith

“…Immune cell infiltration was assessed via flow cytometry, chemokines were analyzed by immunoassays and RNA microarrays. Results were confirmed in specimens from NSCLC patients by flow cytometry or immunohistochemistry as well as in the TCGA data set. …”
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Genetic heterogeneity in familial forms of genetic generalized epilepsy: from mono- to oligogenism by Maha Dahawi, Jean-Madeleine de Sainte Agathe, Mohamed S. Elmagzoub, Elhami A. Ahmed, Julien Buratti, Thomas Courtin, Eric Noé, Julie Bogoin, Bruno Copin, Fatima A. Elmugadam, Wasma A. Abdelgadir, Ahmed K. M. A. Ahmed, Mohamed A. Daldoum, Rayan Mamoon Ibrahim Altayeb, Mohamed Bashir, Leena Mohamed Khalid, Sahar Gamil, Sara Baldassari, Liena Elsayed, Boris Keren, Gregory Nuel, Ammar E. Ahmed, Eric Leguern

“…Moreover, a polygenic mode of inheritance is suspected in most cases. We performed SNP microarrays and whole exome sequencing in 20 families from Sudan, focusing on those with at least four affected members. …”
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TMEM101 expression and its impact on immune cell infiltration and prognosis in hepatocellular carcinoma by Lingyun Kuang, Yilin Pang, Quangang Fang

“…Additionally, differences in TMEM101 expression in HCC cell lines and HCC tissue microarrays were examined using RT-qPCR, western blotting, and in situ hybridization staining. …”
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urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. by Stephen Watt, Juan Mata, Luis López-Maury, Samuel Marguerat, Gavin Burns, Jürg Bähler

“…<h4>Methodology/principal findings</h4>We used S. pombe microarrays to identify three neighbouring genes (urg1, urg2, and urg3) whose transcript levels rapidly and strongly increased in response to uracil, a condition which otherwise had little effect on global gene expression. …”
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Regulation of Proteome Maintenance Gene Expression by Activators of Peroxisome Proliferator-Activated Receptor α by Hongzu Ren, Beena Vallanat, Holly M. Brown-Borg, Richard Currie, J. Christopher Corton

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ESR1 is co-expressed with closely adjacent uncharacterised genes spanning a breast cancer susceptibility locus at 6q25.1. by Anita K Dunbier, Helen Anderson, Zara Ghazoui, Elena Lopez-Knowles, Sunil Pancholi, Ricardo Ribas, Suzanne Drury, Kally Sidhu, Alexandra Leary, Lesley-Ann Martin, Mitch Dowsett

“…Our observations suggest that some of the biological effects previously attributed to ER could be mediated and/or modified by these co-expressed genes. …”
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The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway by Yuexi He, Xiran Yin, Jianjun Yan, Xue Li, Qing Sun

“…Methods. miRNA and lncRNA microarrays were performed using IL-22-induced HaCaT cells and psoriatic lesions, respectively. …”
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LncRNA MBNL1‐AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/PHLPP2/FOXO1 axis in bladder cancer by Xiaosong Wei, Xiaoming Yang, Beibei Wang, Yang Yang, Zhiwei Fang, Chengzhi Yi, Lei Shi, Dongkui Song

“…Abstract LncRNAs have been shown to play essential roles in bladder cancer (BC) progress. Our microarrays of clinical samples firstly screened that lncRNA muscleblind‐like 1 antisense RNA 1 (MBNL1‐AS1) was poorly expressed in BC tissues. …”
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CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer by Xiao Ma, Baojian Zhang, Xuezhe Yin, ShiPeng Yang, Zhenhua Lin, Yang Yang, Xianchun Zhou

“…Methods First, cell functional assays and animal models were employed to elucidate the pro-carcinogenic effects of CPT1A on BC and its role in metabolic alterations. …”
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Associations of circulating insulin-like growth factor-1 and insulin-like growth factor binding protein-3 with the expression of stem cell markers in benign breast tissue by Lusine Yaghjyan, Yujing J. Heng, Brian R. Sardella, Divya Murthy, Matt B. Mahoney, Bernard Rosner, Kornelia Polyak, Maisey Ratcliff, Rulla M. Tamimi

“…Immunohistochemistry (IHC) was done on tissue microarrays. For each core, the IHC expression was assessed using QuPath, and expressed as % of cells that stain positively for a specific marker out of the total cell count. …”
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Genome-wide DNA methylation and gene expression in human placentas derived from assisted reproductive technology by Pauliina Auvinen, Jussi Vehviläinen, Karita Rämö, Ida Laukkanen, Heidi Marjonen-Lindblad, Essi Wallén, Viveca Söderström-Anttila, Hanna Kahila, Christel Hydén-Granskog, Timo Tuuri, Aila Tiitinen, Nina Kaminen-Ahola

“…Methods We performed genome-wide DNA methylation (EPIC Illumina microarrays) and gene expression (mRNA sequencing) analyses for a total of 80 ART and 77 control placentas. …”
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GCKIII kinases control hepatocellular lipid homeostasis via shared mode of action by Emmelie Cansby, Mara Caputo, Emma Andersson, Rasool Saghaleyni, Marcus Henricsson, Ying Xia, Bernice Asiedu, Matthias Blüher, L. Thomas Svensson, Andrew J. Hoy, Margit Mahlapuu

“…Finally, using in vitro kinase assays on microfluidic microarrays, we pinpointed various downstream targets that are phosphorylated by the GCKIII kinases, with known functions in lipogenesis, lipolysis, and lipid secretion, as well as glucose uptake, glycolysis, hexosamine synthesis, and ubiquitination. …”
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