Identification of novel androgen-regulated pathways and mRNA isoforms through genome-wide exon-specific profiling of the LNCaP transcriptome. by Prabhakar Rajan, Caroline Dalgliesh, Phillippa J Carling, Thomas Buist, Chaolin Zhang, Sushma N Grellscheid, Kelly Armstrong, Jacqueline Stockley, Cedric Simillion, Luke Gaughan, Gabriela Kalna, Michael Q Zhang, Craig N Robson, Hing Y Leung, David J Elliott

“…Androgens drive the onset and progression of prostate cancer (PCa) by modulating androgen receptor (AR) transcriptional activity. Although several microarray-based studies have identified androgen-regulated genes, here we identify in-parallel global androgen-dependent changes in both gene and alternative mRNA isoform expression by exon-level analyses of the LNCaP transcriptome. …”
Get full text

Fetal medicine and current practice of prenatal screening by Akshatha Prabhu

“…The fetal sample is used for various genetic tests, such as chromosome microarray analysis (CMA), targeted clinical exome sequencing (CES), or whole exome sequencing (WES) depending upon the abnormality noted or the suspected genetic condition. …”
Get full text

Exploring the predictive values of SERP4 and FRZB in dilated cardiomyopathy based on an integrated analysis by Bin Qi, Hai-Yan Wang, Xiao Ma, Yu-Feng Chi, Chun Gui

“…Methods Five DCM-related microarray datasets were downloaded from the Gene Expression Omnibus (GEO). …”
Get full text

Antibiotic resistance in agroecosystem: Progress and challenges by ZHANG Yusen, YE Jun, SU Jianqiang

“…Both culture-dependent and culture-independent technologies, including metagenomic, microarray and high-throughput quantitative polymerase chain reaction have been utilized in the characterization of ARGs in agroecosystem, and each has its strength and weakness. …”
Get full text

Dual-targeting and steric hindrance resolution in HER2 IHC: a novel approach to improve diagnostic sensitivity by Li Luo, Xi Zhang, Linqiong Chen, Zhuohan Chen, Yuchen Wang, Kaihao Huang, Xiaoyun Lin, Hongxiang Zhu, Wangqi Du

“…We assessed the detection performance of Nby-Aby in BC tissues and compared it with conventional HER2 diagnostic antibodies using tissue microarrays (TMAs). …”
Get full text

Identification of Novel Biomarkers in Huntington’s Disease Based on Differential Gene Expression Meta-Analysis and Machine Learning Approach by Nayan Dash, Md Abul Bashar, Jeonghan Lee, Raju Dash

“…Therefore, to discover the key biomarkers associated with the progression of HD, we employed bioinformatics and machine learning (ML) to create a robust pipeline that integrated differentially expressed gene (DEG) analysis with ML to select potential biomarkers. We performed a meta-analysis to identify DEGs using three Gene Expression Omnibus (GEO) microarray datasets from different platforms related to HD-affected brain tissue, applying both relaxed and strict criteria to identify differentially expressed genes. …”
Get full text

Distinctive profile of IsomiR expression and novel microRNAs in rat heart left ventricle. by Mary K McGahon, Janet M Yarham, Aideen Daly, Jasenka Guduric-Fuchs, Lyndsey J Ferguson, David A Simpson, Anthony Collins

“…The expression of known miRNAs in the heart has been studied at length by microarray and quantitative PCR but it is becoming evident that microRNA isoforms (isomiRs) are potentially physiologically important. …”
Get full text

Circulating exosomal microRNAs as biomarkers of colon cancer. by Hiroko Ogata-Kawata, Masashi Izumiya, Daisuke Kurioka, Yoshitaka Honma, Yasuhide Yamada, Koh Furuta, Toshiaki Gunji, Hideki Ohta, Hiroyuki Okamoto, Hikaru Sonoda, Masatoshi Watanabe, Hitoshi Nakagama, Jun Yokota, Takashi Kohno, Naoto Tsuchiya

“…<h4>Experimental design</h4>Microarray analyses of miRNAs in exosome-enriched fractions of serum samples from 88 primary CRC patients and 11 healthy controls were performed. …”
Get full text

Omics Multi-Layers Networks Provide Novel Mechanistic and Functional Insights Into Fat Storage and Lipid Metabolism in Poultry by Farzad Ghafouri, Abolfazl Bahrami, Abolfazl Bahrami, Mostafa Sadeghi, Seyed Reza Miraei-Ashtiani, Maryam Bakherad, Herman W. Barkema, Samantha Larose

“…In the analysis of microarray and RNA-Seq data, 1,835 genes were detected by comparing the identified genes with significant expression differences (p.adjust &lt; 0.01, fold change ≥ 2 and ≤ −2). …”
Get full text

Alternative splicing of TCF7L2 gene in omental and subcutaneous adipose tissue and risk of type 2 diabetes. by Ludmila Prokunina-Olsson, Lee M Kaplan, Eric E Schadt, Francis S Collins

“…A pathway enrichment analysis on transcripts significantly co-expressed with TCF7L2 in a microarray set combined with individual expression assays, suggested tissue-specific roles of TCF7L2 splicing forms in regulation of transcription, signal transduction and cell adhesion.…”
Get full text

Integrated bioinformatic analysis of the shared molecular mechanisms between ANCA-associated vasculitis and atherosclerosis by Xun Hu, Inmaculada Xu Lou, Qilan Chen

“…This study aims to explore the mechanisms underlying the concurrent occurrence of ANCA and AS. Methods Microarray data of AAV and AS were examined in a comprehensive gene expression database. …”
Get full text

Comparison of monocyte gene expression among patients with neurocysticercosis-associated epilepsy, Idiopathic Epilepsy and idiopathic headaches in India. by Vasudevan Prabhakaran, Douglas A Drevets, Govindan Ramajayam, Josephine J Manoj, Michael P Anderson, Jay S Hanas, Vedantam Rajshekhar, Anna Oommen, Hélène Carabin

“…Fifteen genes up-regulated in NCC patients compared with other groups were selected based on possible relevance to NCC, and analyzed by qPCR in all patients' samples. …”
Get full text

Long-Term Oocyte-Like Cell Development in Cultures Derived from Neonatal Marmoset Monkey Ovary by Bentolhoda Fereydouni, Gabriela Salinas-Riester, Michael Heistermann, Ralf Dressel, Lucia Lewerich, Charis Drummer, Rüdiger Behr

Get full text

Insights from lncRNAs Profiling of MIN6 Beta Cells Undergoing Inflammation by Chuntao Sun, Lihua Xue, Ziyang Zhu, Fan Zhang, Ruixue Yang, Xuewen Yuan, Zhanjun Jia, Qianqi Liu

“…In this study, lncRNA microarray technology was used to identify the differently expressed lncRNAs and mRNAs in MIN6 cells exposed to proinflammatory cytokines. …”
Get full text

Oncolytic virotherapy provides a potent therapy option for squamous bladder cancer by Julia Pannhausen, Julia Wirtz, Klaus Mantwill, Per-Sonne Holm, Kristina Schwamborn, Danny D. Jonigk, Jürgen E. Gschwend, Michael Rose, Nadine T. Gaisa, Roman Nawroth

“…Furthermore, immunohistochemical staining of 89 SCC on a tissue microarray confirmed strong YB-1 expression in squamous BLCA (sq-BLCA). …”
Get full text

Integrative bioinformatic analysis identifies differentially expressed gene targets as potential biomarkers for anaplastic thyroid cancer by Angel Sebastian Treviño-Juarez, Jose Gerardo Gonzalez-Gonzalez, Rene Rodriguez-Gutierrez, Adriana Sanchez-Garcia, Camilo Daniel Gonzalez-Velazquez

“…Methods We analyzed five microarray datasets from the GEO database to compare gene expression profiles between ATC samples and normal thyroid tissues. …”
Get full text

Characterization of Human Knee and Chin Adipose-Derived Stromal Cells by Magali Kouidhi, Phi Villageois, Carine M. Mounier, Corinne Ménigot, Yves Rival, David Piwnica, Jérôme Aubert, Bérengère Chignon-Sicard, Christian Dani

“…Paired chin and knee fat depots were harvested from 11 subjects and gene expression profiles were determined by DNA microarray analyses. Adipose-derived stromal cells (ASCs) from both sites were isolated and analyzed for their capacity to proliferate, form clones, and differentiate. …”
Get full text

Genome-wide and rna-seq highlight genetic characteristics of rumpless signals in piao chicken by Wang Mei QI, Xing Fu ZHANG, Li Wen SONG, Zai Xia LIU, Yuan CHAI, Yan Yong SUN

“…Understanding the genetic relationship between rumpless chicken and other specific selection target breeds in the process of differentiation of the rumpless signal genes is helpful to reveal the genetic basis of rumplessnesss. …”
Get full text

Prenatal Diagnosis of Complex Copy Number Variants in the Fetus and Associated Cytogenetic Findings in Parents by Wei Wang, Jing Wang, Ye Shi, Bin Zhang

“…Methods: 2746 pregnant women were included and diagnosed by chromosomal microarray analysis (CMA) according to different clinical indications. …”
Get full text

Tissue ischemia time affects gene and protein expression patterns within minutes following surgical tumor excision by Annika Spruessel, Garnet Steimann, Mira Jung, Sung A. Lee, Theresa Carr, Anne-Kristin Fentz, Joerg Spangenberg, Carsten Zornig, Hartmut H. Juhl, Kerstin A. David

“…Gene and protein expression were determined by microarray (HG-U133A chips) and surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) technology (CM10 chips, SAX2 chips, and IMAC3Ni chips), respectively. …”
Get full text