Plasma CircCYP24A1 as a Novel Biomarker of Esophageal Squamous Cell Carcinoma by Ruolan Zhang M.M., Jianlin Liu M.M., Hang Yang M.M., Jinsong Tan M.M., Rong Xiong M.M., Yun Liu MM, Gang Feng PhD, Guiqin Song PhD, Kang Liu PhD

“…Fluorescence in situ hybridization was employed to detect the expression of circCYP24A1 in ESCC tissue microarrays, aiming to assess the expression of circCYP24A1 in a large population and its correlation with clinical indicators. …”
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Integration of histopathological images and immunological analysis to predict M2 macrophage infiltration and prognosis in patients with serous ovarian cancer by Ling Zhao, Jiajia Tan, Qiuyuan Su, Yan Kuang, Yan Kuang

“…In addition, tissue microarrays from 106 patients with SOC patients were included as an external validation set. …”
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WD40 Protein NLE1 as a Novel Diagnostic Biomarker Promoting Hepatocellular Carcinoma Proliferation by Rutong Zhang, Dehua Liu, Xiaoxi Feng, Zhenye Yang, Kaiguang Zhang

“…We further evaluated NLE1 expression in patient tissue microarrays. Kaplan−Meier plots, Cox regression analyses, and receiver operating characteristic (ROC) curves were used to evaluate the prognostic relevance of NLE1. …”
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Imputation of variants from the 1000 Genomes Project modestly improves known associations and can identify low-frequency variant-phenotype associations undetected by HapMap based i... by Andrew R Wood, John R B Perry, Toshiko Tanaka, Dena G Hernandez, Hou-Feng Zheng, David Melzer, J Raphael Gibbs, Michael A Nalls, Michael N Weedon, Tim D Spector, J Brent Richards, Stefania Bandinelli, Luigi Ferrucci, Andrew B Singleton, Timothy M Frayling

“…Genome-wide association (GWA) studies have been limited by the reliance on common variants present on microarrays or imputable from the HapMap Project data. …”
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A nomogram for predicting 3-year total weight loss percentage following LSG: insights from visceral adipose tissue inflammatory methylation sites by Zhehong Li, Liang Wang, Zheng Wang, Qiqige Wuyun, Buhe Amin, Dongbo Lian, Guangzhong Xu, Nengwei Zhang, Dezhong Wang

“…Comparative analyses of 850K methylation microarrays from VAT samples were performed to identify methylation sites associated with INF-related genes. …”
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Optical genome mapping with whole genome sequencing identifies complex chromosomal structural variations in acute leukemia by Meng-Ju Melody Tsai, Meng-Ju Melody Tsai, Hsiao-Jung Kao, Hsiao-Huei Chen, Chih-Hsiang Yu, Yin-Hsiu Chien, Wuh-Liang Hwu, Wuh-Liang Hwu, Wuh-Liang Hwu, Pui-Yan Kwok, Pui-Yan Kwok, Pui-Yan Kwok, Ni-Chung Lee, Yung-Li Yang, Yung-Li Yang, Yung-Li Yang

“…However, many complex SVs cannot be identified by conventional tools, including karyotyping, fluorescence in situ hybridization, microarrays, and multiplex ligation-dependent probe amplification (MLPA).MethodsOptical genome mapping (OGM) and whole genome sequencing (WGS) were employed to analyze five leukemia samples with SVs detected by karyotyping, MLPA, and RNA sequencing (RNA-seq). …”
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Inflammatory markers after supplementation with marine n-3 or plant n-6 PUFAs: A randomized double-blind crossover study by Elise Grytten, Johnny Laupsa-Borge, Kaya Cetin, Pavol Bohov, Jan Erik Nordrehaug, Jon Skorve, Rolf K. Berge, Elin Strand, Bodil Bjørndal, Ottar K. Nygård, Espen Rostrup, Gunnar Mellgren, Simon N. Dankel

“…These data point to tissue-specific proinflammatory effects of high n-6 intake, but a net systemic anti-inflammatory effect as for n-3.…”
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Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer. by Jie Chen, Ana R Gomes, Lara J Monteiro, San Yu Wong, Lai Han Wu, Ting-Ting Ng, Christina T Karadedou, Julie Millour, Ying-Chi Ip, Yuen Nei Cheung, Andrew Sunters, Kelvin Y K Chan, Eric W-F Lam, Ui-Soon Khoo

“…<h4>Methodology/principal findings</h4>Examination of FOXO3a and phosphorylated-Akt (P-Akt) expression in breast cancer tissue microarrays showed nuclear FOXO3a was associated with lymph node positivity (p = 0.052), poor prognosis (p = 0.014), and P-Akt expression in invasive ductal carcinoma. …”
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Elucidation of Dysregulated Pathways Associated With Hypoxia in Oestrogen Receptor‐Negative Breast Cancer by Suad A. K. Shamis, Jean Quinn, Sara Al‐Badran, Molly McKenzie, Phimmada Hatthakarnkul, Gerard Lynch, Guang‐Yu Lian, Warapan Numprasit, Laszlo Romics Jr., Ditte Andersen, Elizabeth Mallon, Donald C. McMillan, Joanne Edwards

“…Methods Immunohistochemistry was performed to identify the prognostic role of CAIX protein expression in ER− tissue microarrays (TMAs) (n = 191). CAIX‐positive samples (n = 37) were transcriptionally profiled by TempO‐Seq and analysed by STRING. …”
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Vitamin K‐dependent gamma‐carboxyglutamic acid protein 1 promotes pancreatic ductal adenocarcinoma progression through stabilizing oncoprotein KRAS and tyrosine kinase receptor EGF... by Zheng Wu, Qing Ye, Shan Zhang, Li‐Peng Hu, Xiao‐Qi Wang, Lin‐Li Yao, Lei Zhu, Shu‐Yu Xiao, Zong‐Hao Duan, Xue‐Li Zhang, Shu‐Heng Jiang, Zhi‐Gang Zhang, De‐Jun Liu, Dong‐Xue Li, Xiao‐Mei Yang

“…Methods Analysis of pancreatic ductal adenocarcinoma (PDAC) datasets, including transcription profiles, clinical data, and tissue microarrays, was conducted to evaluate PRRG1 expression and its clinical relevance. …”
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UBE2N as a novel prognostic and therapeutic biomarker of lung adenocarcinoma by Haofeng Yin, Yibo Xue, Chen Wang, Yanqin Wu, Yuchen Guo, Chunzhen Li, Yunyan Zhang, Shulei Yin, Tiejun Zhao

“…Immunohistochemistry, cell viability, and apoptosis experiments were conducted to verify the oncogenic effects of UBE2N.ResultsUBE2N was identified as an independent prognostic biomarker for LUAD. …”
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Integrated hepatic transcriptomics and metabolomics identify Pck1 as a key factor in the broad dysregulation induced by vehicle pollutants by Gajalakshmi Ramanathan, Yuqi Zhao, Rajat Gupta, Siri Langmo, May Bhetraratana, Fen Yin, Will Driscoll, Jerry Ricks, Allen Louie, James A. Stewart, Timothy R. Gould, Timothy V. Larson, Joel Kaufman, Michael E. Rosenfeld, Xia Yang, Jesus A. Araujo

“…Methods Apolipoprotein E knockout (ApoE KO) mice were exposed to DE or filtered air (FA) for two weeks, or DE for two weeks followed by FA for 1 week. Expression microarrays and global metabolomics assessment were performed in the liver. …”
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Effector T cells under hypoxia have an altered transcriptome similar to tumor-stressed T cells found in non-responsive melanoma patients by Ahmad A Tarhini, Zhihua Chen, Dongliang Du, Joshua Davis, Justin C Boucher, Katarzyna A Rejniak, Jose A Guevara-Patino, Timothy I Shaw, Alyssa Obermayer, Mate Z Nagy, Lourdes B Plaza-Rojas, Elena Kostenko, Anna L Austin, Awino Maureiq E Ojwang'

“…As T cell-based therapies often fail to work in solid tumors, this study aims to investigate the effects of hypoxia on T cell topo-distribution in the TME, gene expression association with T cell states, and clinical responses in melanoma.Methods To generate detailed information on tumor oxygenation and T cell accessibility, we used mathematical modeling of human melanoma tissue microarrays that incorporate oxygen supply from vessels, intratumoral diffusion, and cellular uptake. …”
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