Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling by Nikole J. Byrne, Christoph Koentges, Elisabeth Khan, Katharina Pfeil, Robert Sandulescu, Sayan Bakshi, Carolin Költgen, Ivan Vosko, Johannes Gollmer, Thomas Rathner, Günter Roth, Michael M. Hoffmann, Katja E. Odening, Hauke Horstmann, Luke A. Potter, Christoph Bode, Dennis Wolf, Harald Sourij, Senka Ljubojevic-Holzer, Markus Wallner, Peter P. Rainer, Simon Sedej, Daniel Scherr, Dirk von Lewinski, Adam R. Wende, Andreas Zirlik, Heiko Bugger

“…To current study was designed to explore mechanisms underlying adverse effects of increased SIRT4 levels in the heart following pressure overload. …”
Get full text

WTAP/YTHDF1-mediated m6A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs by Quan Chen, Luoquan Ao, Qing Zhao, Lu Tang, Yanli Xiong, Yuchuan Yuan, Xiaofeng Wu, Wei Xing, Zhan Li, Wei Guo, Huaping Liang, Song Guo Zheng, Qizhou Lian, Di Lu, Weijun Wan, Xiang Xu

“…Methods: Epitranscriptomic microarray analysis and MeRIP-qPCR assay were performed to identify the regulatory effect of WTAP in the IFN-γ-licensing MSCs. …”
Get full text

IL-6 is a prognostic biomarker in patients with advanced esophageal squamous cell carcinoma received with PD-1 inhibitors by Ping Huang, Manyi Zhao, Jianhong Xia, Hongliang Li, Junxia Sun, Xin Li, Chunsheng Yang, Guangyi Gao, Wenhang Zhou, Meifeng Zhong, Hongmei Yong

“…BackgroundDue to the low efficacy rates, effective biomarkers are desperately needed to determine populations of advanced esophageal squamous cell carcinoma (ESCC) that may benefit from immune checkpoint inhibitor (ICI) treatment.ObjectivesTo explore the relationship between IL-6 and the esophageal cancer tumor immune microenvironment using online databases and esophageal cancer tissue microarrays and to investigate the predictive role of IL-6 for immunotherapy in esophageal squamous carcinoma patients through clinical study data.MethodsRNA-seq datasets of ESCC patients were obtained from TCGA, and the relationship between IL-6 and immune cells was discovered using TIMER 2.0 databases. …”
Get full text

Analysis of Result Variability from High-Density Oligonucleotide Arrays Comparing Same-Species and Cross-Species Hybridizations by J.D. Chismar, T. Mondala, H.S. Fox, E. Roberts, D. Langford, E. Masliah, D.R. Salomon, S.R. Head

“…There exists a significant limitation in the variety of organisms for which microarrays have been developed because of a lack of genomic sequence data. …”
Get full text

Adrenocortical carcinoma survival gene HMMR was identified as being targeted by fluorouracil and epirubicin using a gene coexpression network-based drug repositioning strategy by Zahra Jafari, Seyed-Morteza Javadirad, Seyede Elmira Yazdi Rouholamini

“…Drug repositioning based on gene coexpression networks has proven to be an effective method of discovering potential cancer treatments. …”
Get full text

The antiprotease Spink7 promotes inflammation resolution by modulating multiple proteases activities during wound healing by Na Zhao, Guojian Wang, Shuang Long, Xiaofan Lv, Xinze Ran, Junping Wang, Yongping Su, Tao Wang

“…Abstract Background Effective control of inflammation is crucial for the healing of cutaneous wounds, but the molecular mechanisms governing inflammation resolution during wound closure are still not yet clear. …”
Get full text

Comprehensive analysis of the clinical and biological significances of polyamine metabolism in glioma by Fei Bai, Kexin Zhai, Tiantian Yao, Yuxia Zhou, Yongxiang Zhao, Guanghui Yang

“…What’s more, IHC staining was conducted on glioma tissue microarrays to confirm the expression patterns of genes related to polyamine metabolism in glioma tissues. …”
Get full text

The relative resistance of children to sepsis mortality: from pathways to drug candidates by Rose B Joachim, Gabriel M Altschuler, John N Hutchinson, Hector R Wong, Winston A Hide, Lester Kobzik

“…To find drugs that could promote beneficial (child) pathways or inhibit harmful (adult) ones, we built an in silico pathway drug network (PDN) using expression correlation between drug, disease, and pathway gene signatures across 58,475 microarrays. Specific pathway clusters from children or adults were assessed for correlation with drug‐based signatures. …”
Get full text

Integrated analyses of copy number variations and gene expression in lung adenocarcinoma. by Tzu-Pin Lu, Liang-Chuan Lai, Mong-Hsun Tsai, Pei-Chun Chen, Chung-Ping Hsu, Jang-Ming Lee, Chuhsing Kate Hsiao, Eric Y Chuang

“…Identification of prognostic biomarkers for lung cancer using gene expression microarrays poses a major challenge in that very few overlapping genes have been reported among different studies. …”
Get full text

From Insect to Man: Photorhabdus Sheds Light on the Emergence of Human Pathogenicity. by Geraldine Mulley, Michael L Beeton, Paul Wilkinson, Isabella Vlisidou, Nina Ockendon-Powell, Alexia Hapeshi, Nick J Tobias, Friederike I Nollmann, Helge B Bode, Jean van den Elsen, Richard H ffrench-Constant, Nicholas R Waterfield

“…Photorhabdus are highly effective insect pathogenic bacteria that exist in a mutualistic relationship with Heterorhabditid nematodes. …”
Get full text

Diagnosing Autoimmune Bullous Diseases—An Indian Perspective by Adhyatm Bhandari, Dipankar De, Shikha Shah, Debajyoti Chatterjee, Vinod Kumar, Rahul Mahajan, Sanjeev Handa

“…Accurate diagnosis of the specific subtype of AIBD is crucial for effective management and predicting prognosis, especially in cases with an increased risk of malignancy. …”
Get full text

A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. by Davide Danovi, Amos Folarin, Sabine Gogolok, Christine Ender, Ahmed M O Elbatsh, Pär G Engström, Stefan H Stricker, Sladjana Gagrica, Ana Georgian, Ding Yu, Kin Pong U, Kevin J Harvey, Patrizia Ferretti, Patrick J Paddison, Jane E Preston, N Joan Abbott, Paul Bertone, Austin Smith, Steven M Pollard

“…Glioblastoma multiforme (GBM) is the most common primary brain cancer in adults and there are few effective treatments. GBMs contain cells with molecular and cellular characteristics of neural stem cells that drive tumour growth. …”
Get full text

SNPstream® UHT: Ultra-High Throughput SNP Genotyping for Pharmacogenomics and Drug Discovery by Peter A. Bell, Surendra Chaturvedi, Craig A. Gelfand, Ching Yu Huang, Michael Kochersperger, Rafael Kopla, Frank Modica, Mark Pohl, Shobha Varde, Renbin Zhao, Xiaojian Zhao, Michael T. Boyce-Jacino

“…The system employs oligonucleotide microarrays manufactured in a 384-well format on a novel glass-bottomed plate. …”
Get full text

C20orf27 promotes hepatocellular carcinoma progression via NT5E by Yu He, Wen Zhang, Ming Chang, Yuanjie Chen, Risheng Lin, Xinyu Ye, Wei Mao, Yubin Lu, Yi Lu, Erbao Chen

“…Immunohistochemistry analysis of tissue microarrays suggested that NT5E expression was significantly associated with C20orf27 levels. …”
Get full text

Engineered EVs from LncEEF1G - overexpressing MSCs promote fibrotic liver regeneration by upregulating HGF release from hepatic stellate cells by Jiebin Zhang, Xiaotong Qiu, Yunguo Lei, Haitian Chen, Dongwei Wu, Tingting Wang, Xin Sui, Jiaqi Xiao, Chenhao Jiang, Huayao Zhang, Yasong Liu, Xiaoquan Liu, Yingcai Zhang, Xu Che, Ye Lin, Jia Yao, Zihao Pan, Rong Li, Jun Zheng

“…However, there is still no clinically effective treatment for promoting fibrotic liver regeneration because the underlying hepatocellular mechanism remains poorly understood. …”
Get full text

Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers by Joviana Farhat, Lara Alzyoud, Mohammad AlWahsh, Animesh Acharjee, Basem Al‐Omari

“…Results In practice, fluorescence in situ hybridization, karyotype, microarrays and other genetic tests are mainly applied to identify specific genetic alterations and mutations associated with cancer progression. …”
Get full text

Pan-cancer landscape of ITGAV and its potential role in gastric cancer by Bin Ke, Peng Jin, Xue-Jun Wang, Ru-peng Zhang, Ning Liu, Gang Ma

“…Gene enrichment analysis was performed to explore potential functions of ITGAV in gastric cancer (GC). GC tissue microarrays and in vitro cell experiments were used to verify the prediction results in GC. …”
Get full text

Evaluating the potential of anti-dsRNA antibodies as an alternative viral sensing tool in encephalitides of different species by Madeleine de le Roi, Hannah Gerhards, Adnan Fayyad, Adnan Fayyad, Mathias Boelke, Stefanie Christine Becker, Asisa Volz, Ingo Gerhauser, Wolfgang Baumgärtner, Christina Puff

“…Molecular methods like multiplex PCR and microarrays are considered to be often less sensitive than Next Generation Sequencing, whereas the latter is time-consuming and costly. …”
Get full text

A Narrative Review of Molecular, Immunohistochemical and In-Situ Techniques in Dermatopathology by J. A. Gabriel, N. Weerasinghe, P. Balachandran, R. Salih, G. E. Orchard

“…Gene expression profiling techniques, such as microarrays and RNA sequencing, have provided insights into dysregulated pathways and molecular signatures associated with conditions ranging from inflammatory skin disorders to cutaneous malignancies. …”
Get full text

Transcriptomic analysis of neural stem and progenitor cells in comparison with glioblastoma stem cells by V. E. Shevchenko, N. E.  Arnotskaya, T.  I. Kushnir, A. S. Bryukhovetskiy

“…To conduct a comparative transcriptomic analysis of CD133+-NSPCs and CD133+-GSCs to study the molecular genetic differences between the phenotypes of these cells and identify potential targets for therapeutic effects on GSCs.Materials and methods. Used: highly sensitive transcriptomic analysis on high-density microarrays, cellular technologies, modern bioinformatics analysis.Results. …”
Get full text