Inhibitory Effect of PRMT5/MTA Inhibitor on MTAP‐Deficient Glioma May Be Influenced by Surrounding Normal Cells by Yunjie Wang, Xiaohui Sun, Runchen Ma, Xiaofan Zhang, Shengmin Ji, Zhaofeng Liu, Gangqiang Yang, Hongbo Wang, Peng Zhang, Jianzhao Zhang, Jingwei Tian

“…In vitro and in vivo experiments have demonstrated that MRTX1719, a small molecule that selectively binds PRMT5/MTA complex, significantly inhibits the proliferation of MTAP‐deficient tumors and has a weak toxic effect on normal cells. …”
Get full text

Etiology and outcomes of fetal renal abnormalities in Southern China: a single-tertiary-center study by Meiying Cai, Yashi Gao, Huili Xue, Xianguo Fu, Hua Cao, Liangpu Xu, Na Lin, Hailong Huang

“…We retrospectively analyzed data from 1,019 cases for which chromosomal microarray analysis (CMA) was performed; 58 CMA-negative fetuses were selected for whole-exome sequencing (WES). …”
Get full text

A retrospective analysis of 6942 amniocentesis cases by Qingsha An, Yuxiao Huang, Feifei Yu, Yilun Tao, Juan Li, Xiaoze Li

“…Among the single-method cases, Chromosomal Microarray Analysis (CMA) was the most frequently selected technique (14.18%). …”
Get full text

Highly immunoreactive IgG antibodies directed against a set of twenty human proteins in the sera of patients with amyotrophic lateral sclerosis identified by protein array. by Caroline May, Eckhard Nordhoff, Swaantje Casjens, Michael Turewicz, Martin Eisenacher, Ralf Gold, Thomas Brüning, Beate Pesch, Christian Stephan, Dirk Woitalla, Botond Penke, Tamás Janáky, Dezső Virók, László Siklós, Jozsef I Engelhardt, Helmut E Meyer

“…Amyotrophic lateral sclerosis (ALS), the most common adult-onset motor neuron disorder, is characterized by the progressive and selective loss of upper and lower motor neurons. Diagnosis of this disorder is based on clinical assessment, and the average survival time is less than 3 years. …”
Get full text

A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. by Davide Danovi, Amos Folarin, Sabine Gogolok, Christine Ender, Ahmed M O Elbatsh, Pär G Engström, Stefan H Stricker, Sladjana Gagrica, Ana Georgian, Ding Yu, Kin Pong U, Kevin J Harvey, Patrizia Ferretti, Patrick J Paddison, Jane E Preston, N Joan Abbott, Paul Bertone, Austin Smith, Steven M Pollard

“…We used live-cell imaging and high content image analysis tools and identified JNJ-10198409 (J101) as an agent that induces mitotic arrest at prometaphase in GNS cells but not NS cells. Antibody microarrays and kinase profiling suggested that J101 responses are triggered by suppression of the active phosphorylated form of polo-like kinase 1 (Plk1) (phospho T210), with resultant spindle defects and arrest at prometaphase. …”
Get full text

Tumor immune microenvironment permissive to metastatic progression of ING4-deficient breast cancer. by Emily Tsutsumi, Anne M Macy, Janine LoBello, Karen T Hastings, Suwon Kim

“…To characterize the tumor immune microenvironment associated with ING4-deficient tumors, three approaches were employed in this study: First, tissue microarrays composed of 246 primary breast tumors including 97 ING4-deficient tumors were evaluated for the presence of selective immune markers, CD68, CD4, CD8, and PD-1, using immunohistochemical staining. …”
Get full text

urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. by Stephen Watt, Juan Mata, Luis López-Maury, Samuel Marguerat, Gavin Burns, Jürg Bähler

“…The kanMX6 and natMX6 markers allow selection under urg1 induced and repressed conditions, respectively. …”
Get full text

Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis. by Claudia Bühnemann, Simon Li, Haiyue Yu, Harriet Branford White, Karl L Schäfer, Antonio Llombart-Bosch, Isidro Machado, Piero Picci, Pancras C W Hogendoorn, Nicholas A Athanasou, J Alison Noble, A Bassim Hassan

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”
Get full text

History and prospects of flax genetic markers by Daiana A. Zhernova, Elena N. Pushkova, Tatiana A. Rozhmina, Elena V. Borkhert, Alexander A. Arkhipov, Alexander A. Arkhipov, Elizaveta A. Sigova, Elizaveta A. Sigova, Ekaterina M. Dvorianinova, Ekaterina M. Dvorianinova, Alexey A. Dmitriev, Alexey A. Dmitriev, Nataliya V. Melnikova

“…Cost-effective and accurate analysis of large number of genotypes for multiple markers simultaneously using microarrays or targeted deep sequencing became available, as well as HRM, TaqMan, KASP, and other fluorescence-based high-throughput methods for detecting DNA polymorphisms. …”
Get full text

A Narrative Review of Molecular, Immunohistochemical and In-Situ Techniques in Dermatopathology by J. A. Gabriel, N. Weerasinghe, P. Balachandran, R. Salih, G. E. Orchard

“…Moreover, molecular profiling is increasingly guiding the selection of therapeutic agents, ensuring a personalised and effective approach to managing skin disorders. …”
Get full text

A nomogram for predicting 3-year total weight loss percentage following LSG: insights from visceral adipose tissue inflammatory methylation sites by Zhehong Li, Liang Wang, Zheng Wang, Qiqige Wuyun, Buhe Amin, Dongbo Lian, Guangzhong Xu, Nengwei Zhang, Dezhong Wang

“…Comparative analyses of 850K methylation microarrays from VAT samples were performed to identify methylation sites associated with INF-related genes. …”
Get full text

Decoding the prognostic landscape of LUAD: the interplay between N6-methyladenosine modification and immune microenvironment by Quan Chen, Quan Chen, Weijun Wan, Qing Zhao, Juan Li, Juan Li, Yanli Xiong, Yanli Xiong, Yuchuan Yuan, Lu Tang, Xiaofeng Wu, Wei Xing, Wei Guo, Di Lu, Luoquan Ao, Xiang Xu, Xiang Xu, Xiang Xu, Xiang Ao, Xiang Ao, Xiang Ao

“…A risk score model was constructed using least absolute shrinkage and selection operator regression and validated in two independent cohorts and LUAD tissue microarrays. …”
Get full text

Rotterdam Oncology Documentation (RONCDOC) – a high-quality data warehouse and tissue collection for head and neck cancer by Arta Hoesseini, Emilie A. C. Dronkers, Eveline Dieleman, Maria J. De Herdt, Marjan. H. Wieringa, Marie-Louise F. van Velthuysen, Marinella P. J. Offerman, Aniel Sewnaik, Dominiek Monserez, Stijn Keereweer, Jose A. U. Hardillo, Robert Jan Baatenburg de Jong

“…Furthermore, a comprehensive validation protocol was developed to guarantee the quality of the data. Tissue microarrays (TMAs) were constructed from resection specimens of patients with primary oral squamous cell carcinoma. …”
Get full text

Development and In Vitro Characterization of [³H]GMC-058 as Radioligand for Imaging Parkinsonian-Related Proteinopathies by Andrea Varrone, Vasco C. Sousa, Manolo Mugnaini, Sandra Biesinger, Gunnar Nordvall, Lee Kingston, Ileana Guzzetti, Charles S. Elmore, Dan Sunnemark, Dinahlee Saturnino Guarino, Sjoerd J. Finnema, Magnus Schou

“…[³H]GMC-058 was the only compound with negligible binding in controls, and was further evaluated using tissue microarrays, autoradiography on fresh-frozen brain slices, and in vitro saturation binding assay on brain homogenates. …”
Get full text

Effects of tumour-associated macrophages on cardia carcinoma progression by Zhixiong Zhang, Zihong Xu, Yaowu Cai, Xintong Chen, Chaoqing Huang, Xuefeng Xu

“…Methods Cancer tissue and pericancer tissue samples from 80 patients with cardia carcinoma who underwent D2 radical surgery between January 2014 and January 2015 at Putian First Hospital were selected. Tissue microarrays were fabricated, and immunohistochemistry technology was used to detect the infiltration of TAMs (CD163 as a marker) and to analyse their relationship with the clinicopathological characteristics of the patients. …”
Get full text

MaGIC: a program to generate targeted marker sets for genome-wide association studies by Claire L. Simpson, Valerie K. Hansen, Pak C. Sham, Andrew Collins, John F. Powell, Ammar Al-Chalabi

“…High-throughput genotyping technologies such as DNA pooling and DNA microarrays mean that whole-genome screens are now practical for complex disease gene discovery using association studies. …”
Get full text

Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish. by Gustavo Gomez, Jae-Hyung Lee, Matthew B Veldman, Jing Lu, Xinshu Xiao, Shuo Lin

“…Expression studies of 50 selected candidate genes from this dataset resulted in the identification of 39 new genes that are expressed in vascular cells. …”
Get full text

The transcription factor SOX18 regulates the expression of matrix metalloproteinase 7 and guidance molecules in human endothelial cells. by Martina Hoeth, Heide Niederleithner, Renate Hofer-Warbinek, Martin Bilban, Herbert Mayer, Ulrike Resch, Christof Lemberger, Oswald Wagner, Erhard Hofer, Peter Petzelbauer, Rainer de Martin

“…<h4>Methodology/principal findings</h4>SOX18 was expressed in HUVEC using a recombinant adenoviral vector and the altered gene expression profile was analyzed using microarrays. Expression of several regulated candidate SOX18 target genes was verified by real-time PCR. …”
Get full text

Comprehensive analysis of microRNA expression profile in malignant glioma tissues by Monika Piwecka, Katarzyna Rolle, Agnieszka Belter, Anna Maria Barciszewska, Marek Żywicki, Marcin Michalak, Stanisław Nowak, Mirosława Z. Naskręt-Barciszewska, Jan Barciszewski

“…We investigated the global miRNA profile of malignant glioma tissues with miRNA microarrays, deep sequencing and meta‐analysis. We selected miRNAs that were most frequently deregulated in glioblastoma tissues, as well as in peritumoral areas, in comparison with normal human brain. …”
Get full text

Revealing new mouse epicardial cell markers through transcriptomics. by Lars Bochmann, Padmini Sarathchandra, Federica Mori, Enrique Lara-Pezzi, Domenico Lazzaro, Nadia Rosenthal

“…<h4>Methodology</h4>Using laser capture microscopy, we isolated the epicardial layer from the adult murine heart before or after cardiac infarction in wildtype mice and mice expressing a transgenic IGF-1 propeptide (mIGF-1) that enhances cardiac repair, and analyzed the transcription profile using DNA microarrays.<h4>Principal findings</h4>Expression of epithelial genes such as basonuclin, dermokine, and glycoprotein M6A are highly enriched in the epicardial layer, which maintains expression of selected embryonic genes involved in epicardial development in mIGF-1 transgenic hearts. …”
Get full text