Circular RNA APP contributes to Alzheimer’s disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis by Deng-Pan Wu, Yan-Su Wei, Li-Xiang Hou, Yu-Xuan Du, Qiu-Qing Yan, Ling-Ling Liu, Yuan-Dan Zhao, Ru-Yu Yan, Chao Yu, Zhen-Guo Zhong, Jin-Lan Huang

“…RT-PCR assay was conducted to detect the expression of circAPP, miR-1906 and CLIC1. …”
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Large-scale genome-wide association studies and meta-analyses of longitudinal change in adult lung function. by Wenbo Tang, Matthew Kowgier, Daan W Loth, María Soler Artigas, Bonnie R Joubert, Emily Hodge, Sina A Gharib, Albert V Smith, Ingo Ruczinski, Vilmundur Gudnason, Rasika A Mathias, Tamara B Harris, Nadia N Hansel, Lenore J Launer, Kathleen C Barnes, Joyanna G Hansen, Eva Albrecht, Melinda C Aldrich, Michael Allerhand, R Graham Barr, Guy G Brusselle, David J Couper, Ivan Curjuric, Gail Davies, Ian J Deary, Josée Dupuis, Tove Fall, Millennia Foy, Nora Franceschini, Wei Gao, Sven Gläser, Xiangjun Gu, Dana B Hancock, Joachim Heinrich, Albert Hofman, Medea Imboden, Erik Ingelsson, Alan James, Stefan Karrasch, Beate Koch, Stephen B Kritchevsky, Ashish Kumar, Lies Lahousse, Guo Li, Lars Lind, Cecilia Lindgren, Yongmei Liu, Kurt Lohman, Thomas Lumley, Wendy L McArdle, Bernd Meibohm, Andrew P Morris, Alanna C Morrison, Bill Musk, Kari E North, Lyle J Palmer, Nicole M Probst-Hensch, Bruce M Psaty, Fernando Rivadeneira, Jerome I Rotter, Holger Schulz, Lewis J Smith, Akshay Sood, John M Starr, David P Strachan, Alexander Teumer, André G Uitterlinden, Henry Völzke, Arend Voorman, Louise V Wain, Martin T Wells, Jemma B Wilk, O Dale Williams, Susan R Heckbert, Bruno H Stricker, Stephanie J London, Myriam Fornage, Martin D Tobin, George T O'Connor, Ian P Hall, Patricia A Cassano

“…Publicly available microarray data confirmed differential expression of all three genes in lung samples from COPD patients compared with controls. …”
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CD226+ B cells in primary Sjögren’s syndrome: a key player in clinical manifestations and disease pathogenesis by Ping Zhao, Ping Zhao, Saizhe Song, Song Zhang, Cheng Peng, Wei Cheng, Xin Chang, Xin Chang, Changhao Xie, Zhongli Hu, Cuiping Liu

“…Multicolor immunofluorescence staining was applied to detect the co-expression of B cells and CD226 in the salivary gland of pSS patients.Microarray analysis was conducted to analyze the transcriptomic profiles of sorted CD226+ CD19+ B cells and CD226- CD19+ B cells.ResultsCD226 expression in the peripheral blood of pSS patients was significantly increased on T cells, CD19+ B cells and CD14+ monocytes, but significantly decreased on CD56+ NK cells.We identified a distinct CD226+CD19+ B cell subset that exhibited pathogenic features in pSS. …”
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A novel polypeptide encoded by circSPIRE1 promotes prostate cancer proliferation and migration by restraining the ubiquitin-dependent degradation of LRP5 by Jintao Hu, Juanyi Shi, Junjie Wang, Yunfei Xiao, Degeng Kong, Mingchao Gao, Tianlong Luo, Shizhong Xu, Zhihan Yuan, Xinyi Ma, Xueseng Dong, Jingang Huang, Cheng Liu, Kewei Xu

“…This study characterizes a novel circRNA-encoded protein, rtSPIRE1, and investigates its mechanistic role in prostate cancer proliferation and migration, as well as its diagnostic and therapeutic potential. Methods RNA microarray identified circSPIRE1 in prostate cancer tissues. …”
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circKCNQ5 promotes the proliferation of DNA-methyltransferase 3A R882 mutated acute myeloid leukemia cells by elevating high-mobility group box 1 expression by Yijian Chen, Xiaodan Zhu, Chuanming Lin, Rong Xu, Pengxiang Xu, Liuyan Xin, Lin Li, Liqun Zhang

“…Background Patients with acute myeloid leukemia (AML) harboring the DNA-methyltransferase 3 A (DNMT3A) R882 mutation (DR882MUT) usually have a high recurrence rate and poor prognosis. circKCNQ5 levels were aberrantly elevated in patients with AML according to the microarray platform. Therefore, the purpose of this study is to investigate the effect and mechanism of circKCNQ5 on DR882MUT AML cell proliferation.Methods A DR882MUT cell line model was established. circKCNQ5 expression in AML cells expressing wild-type DNMT3A (DNMT3A-WT) or DR882MUT was analyzed using RT-qPCR. …”
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The NR_109/FUBP1/c-Myc axis regulates TAM polarization and remodels the tumor microenvironment to promote cancer development by Baoen Shan, Cong Zhang, Guogui Sun, Hongtao Zhang, Xiaoya Li, Sisi Wei, Suli Dai, Huixia Wang, Lianmei Zhao

“…Long noncoding RNAs (lncRNAs) have been implicated in various physiological and pathological processes, yet the underlying mechanism on how lncRNAs manipulate the polarization states of TAMs is still unclear and remains to be further investigated.Methods Microarray analyses were employed to characterize the lncRNA profile involved in THP-1-induced M0, M1 and M2-like macrophage. …”
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Identifying critical modules and biomarkers of intervertebral disc degeneration by using weighted gene co‐expression network by Daqian Zhou, Tao Liu, Yongliang Mei, Jiale Lv, Kang Cheng, Weiye Cai, Silong Gao, Daru Guo, Xianping Xie, Zongchao Liu

“…Additionally, we predicted miRNAs involved in hub gene co‐regulation and analyzed miRNA microarray data from GSE116726 to identify four differentially expressed miRNAs. …”
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PARP9-PARP13-PARP14 axis tunes colorectal cancer response to radiotherapy by Rimvile Prokarenkaite, Karolina Kuodyte, Greta Gudoityte, Elzbieta Budginaite, Daniel Naumovas, Egle Strainiene, Kristijonas Velickevicius, Audrius Dulskas, Ernestas Sileika, Jonas Venius, Virginijus Tunaitis, Augustas Pivoriunas, Vytaute Starkuviene, Vaidotas Stankevicius, Kestutis Suziedelis

“…The transcriptomes of nonirradiated and irradiated cells were analyzed using microarrays. Gene set enrichment analysis was conducted to determine the pathways in which PARP13 is engaged. …”
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Disruption of bioenergetics enhances the radio-sensitivity of patient-derived glioblastoma tumorspheres by Seo Jin Kim, Junseong Park, Jin-kyoung Shim, Ran Joo Choi, Ju Hyung Moon, Eui Hyun Kim, Wan-Yee Teo, Jong Hee Chang, Seok-Gu Kang

“…Methods: Gene expression profiles of GBM patient-derived TSs were obtained through microarray and RNA-seq. In vitro treatment efficacy was assessed using clonogenic assay, 3D invasion assay, neurosphere formation assay, and flow cytometry. …”
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Autoantibodies Targeting Vinculin Reveal Novel Insight into the Mechanisms of Autoimmune Podocytopathies by Hanyan Meng, Dongjie Wang, Chen Zheng, Chao Zhou, Xinrui Mao, Jinglan Gu, Xiaohui Qiao, Fei Liu, Jingjing Wang, Haidong Fu, Jianhua Mao, Qing Ye

“…Methods: Serum anti-vinculin autoantibody levels detected by protein microarray and clinical data were compared among INS patients (n = 147), healthy individuals (n = 84), and patients with other kidney or immune diseases (n = 100 of each disease). …”
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Hedgehog Signaling Inhibition by Smoothened Antagonist BMS-833923 Reduces Osteoblast Differentiation and Ectopic Bone Formation of Human Skeletal (Mesenchymal) Stem Cells by Nihal AlMuraikhi, Nuha Almasoud, Sarah Binhamdan, Ghaydaa Younis, Dalia Ali, Muthurangan Manikandan, Radhakrishnan Vishnubalaji, Muhammad Atteya, Abdulaziz Siyal, Musaad Alfayez, Abdullah Aldahmash, Moustapha Kassem, Nehad M. Alajez

“…Global gene expression profiling was performed using the Agilent® microarray platform. Effects on in vivo ectopic bone formation were assessed by implanting hMSC mixed with hydroxyapatite-tricalcium phosphate granules subcutaneously in 8-week-old female nude mice, and the amount of bone formed was assessed using quantitative histology. …”
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Study on the mechanisms and functions of lncRNAs in regulating carboplatin resistance in ovarian cancer cells by HONG Yi, CHEN Cuilan, YIN Fuqiang, LIU Xia

“…Methods Differential expressed lncRNAs were screened using expression profiling microarrays in two pairs of carboplatin-resistant and sensitive OC cells (HeyA8-CBP/HeyA8 and SKOV3-CBP/SKOV3). …”
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GATA6 immunohistochemistry and prognosis after surgical resection of pancreatic adenocarcinoma: results from the ESPAC-4 trial by R.C. Grant, K. Duan, R. Jackson, W. Greenhalf, E. Costello-Goldring, P. Ghaneh, C. Halloran, D. Palmer, T. Hackert, M. Büchler, S. Hutchinson, S. Ramotar, A. Dodd, J. Wilson, F. Notta, G. O’Kane, J. Knox, J. Neoptolemos, S. Gallinger, S.E. Fischer

“…Two pathologists independently assessed GATA6 protein expression by immunohistochemistry in tissue microarray cores, manually and with digital assistance. …”
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Repurposing MDM2 inhibitor RG7388 for TP53-mutant NSCLC: a p53-independent pyroptotic mechanism via ROS/p-p38/NOXA/caspase-3/GSDME axis by Gaoyan Tang, Xuelei Cao, Jiaqi Chen, Fu Hui, Na Xu, Yiqing Jiang, Hongmin Lu, Haifeng Xiao, Xiuming Liang, Mingzhe Ma, Yu Qian, Dongli Liu, Zhenlu Wang, Shuzhen Liu, Guohua Yu, Lei Sun

“…Furthermore, immunohistochemical (IHC) analysis of an NSCLC tissue microarray confirmed a strong positive correlation between p-p38 and NOXA expression. …”
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Oncolytic adenovirus decreases the proportion of TIM-3+ subset of tumor-infiltrating CD8+ T cells with correlation to improved survival in patients with cancer by Matti Kankainen, Timo Joensuu, Akseli Hemminki, Juuso Juhila, Siri Tähtinen, Otto Hemminki, Anna Kanerva, Kristian Taipale, Ilkka Liikanen, Minna Oksanen, Dafne C A Quixabeira, Saru Basnet

“…Meanwhile, less is known about their effects on immune checkpoints, the main targets for passive immunotherapy of cancer. …”
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Differential expression pattern of CC chemokine receptor 7 guides precision treatment of hepatocellular carcinoma by Jie Qin, Qianyi Gong, Cheng Zhou, Jietian Xu, Yifei Cheng, Weiyue Xu, Di Zhu, Yiming Liu, Yuye Zhang, Yanru Wang, Lingling Gao, Lanfang Li, Wulei Hou, Qian Li, Binbin Liu, Yazhen Zhu, Zuoyun Wang, Jieyi Shi, Shuangjian Qiu, Chunmin Liang

“…In this study, the complex tumor microenvironment was identified by using tissue microarray (TMA), spatial transcriptomes and single-cell sequencing. …”
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Decoding chromosomal instability insights in CRC by integrating omics and patient-derived organoids by Federica Papaccio, Manuel Cabeza-Segura, Blanca García-Micó, Francisco Gimeno-Valiente, Sheila Zúñiga-Trejos, Valentina Gambardella, María Fernanda Gutiérrez‐Bravo, Carolina Martinez‐Ciarpaglini, Pilar Rentero‐Garrido, Tania Fleitas, Susana Roselló, Juan Antonio Carbonell-Asins, Marisol Huerta, David Moro-Valdezate, Desamparados Roda, Noelia Tarazona, Manuel M. Sánchez del Pino, Andrés Cervantes, Josefa Castillo

“…From a clinical perspective, a better knowledge of these tumour’s biology will help to guide therapeutic strategies more effectively. Methods We used high-density chromosomal microarray analysis to evaluate CIN level of patient-derived organoids (PDOs) and their original mCRC tissues. …”
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Variations in salivary microbiome and metabolites are associated with immunotherapy efficacy in patients with advanced NSCLC by DanHui Huang, YueHua Chen, Cui Li, Shuang Yang, LiShan Lin, XiaoNan Zhang, XiaoFang Su, LaiYu Liu, Haijin Zhao, Tingyue Luo, Shaoxi Cai, QianNan Ren, Hangming Dong

“…Besides, in vitro experiments and tumor tissue microarray, including 70 NSCLC patients, were utilized to further explore the potential mechanism linking the oral microbiome and immunotherapy efficacy. …”
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LAPTM4B enhances the stemness of CD133+ liver cancer stem-like cells via WNT/β-catenin signaling by Jiahong Wang, Jianping Liao, Ye Cheng, Meirong Chen, Aimin Huang

“…Therefore, we investigated the effects of LAPTM4B on the stemness regulation of cluster of differentiation 133 (CD133)+ liver cancer stem-like cells (CSLCs). …”
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Aldo-keto reductase family 1 member B10 prevents esophageal squamous cell carcinoma from reactive carbonyl species-induced cell death and promotes its progression by Shau-Hsuan Li, Wan-Ting Huang, Yen-Hao Chen, Hung-I Lu, Chien-Ming Lo, Hsin-Ting Tsai, Chang-Han Chen

“…Furthermore, the identified signaling pathway and its biological effects were investigated in ESCC cell lines in vitro and 4-nitroquinoline 1-oxide (4-NQO)-induced-ESCC murine model in vivo. …”
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