Expression and prognostic significance of MLH1 and GPRC5C in resectable hepatocellular carcinoma by Jun Lu, Lei Li, Qing Chen, Bingqi Li, Siqian Ren, Meng Yuan, Meng Meng, Jian Lei, Yuntao Bing, Hangyan Wang, Zhaolai Ma, Dianrong Xiu, Chunhui Yuan

“…However, their expression and prognostic significance in hepatocellular carcinoma (HCC) has not been elucidated, especially for GPRC5C. Methods We detected MLH1 and GPRC5C expression using the tissue microarray-based immunohistochemical staining in tumor and matched adjacent non-tumor liver (ANL) specimens from 230 patients who underwent radical resection for HCC. …”
Get full text

Cytokine and Antibody Based Diagnostic Algorithms for Sputum Culture-Positive Pulmonary Tuberculosis. by Tao Chen, Jinfei Lin, Wei Wang, Joy Fleming, Liang Chen, Yunxia Wang, Haicheng Li, Huixin Guo, Jie Zhou, Xunxun Chen, Yuhui Chen, Qinghua Liao, Yang Shu, Yaoju Tan, Meiling Yu, Guozhou Li, Lin Zhou, Qiu Zhong, Lijun Bi, Lina Guo, Meigui Zhao

“…TB-related cytokines and TB-specific antibodies were detected in parallel with microarray technology. The diagnostic performance of the new protocol for active TB was systematically compared with other traditional methods.…”
Get full text

Interferon-stimulated circHOMER1 attenuates antiviral innate immunity by Ao Zhang, Ranran Yao, Shijin Geng, Haiying Wang, Rong-Chun Tang, Hengxiang Yu, Rui Xu, Hong-Yan Chen, Yunxuan Zhou, Lan Zhang, Jun Zhang

“…Furthermore, circHOMER1 was found to disrupt the interaction between Ras GTPase-activating protein-binding protein 1 (G3BP1) and retinoic-acid-inducible protein I (RIG-I), impairing the detection of viral RNAs by RIG-I. Notably, the concordant immunosuppressive effects of circHOMER1 were observed in mouse infection models. …”
Get full text

Knockdown of A-kinase anchor protein 4 inhibits proliferation of triple-negative breast cancer cells and by Nirmala Jagadish, Sonika Devi, Namita Gupta, Vitusha Suri, Anil Suri

Get full text

A systems biological approach reveals multiple crosstalk mechanism between gram-positive and negative bacterial infections: an insight into core mechanism and unique molecular sign... by R Muthukumar, V Alexandar, Berla Thangam, Shiek S S J Ahmed

“…Further, to identify candidate molecules, the TLRs specific nodes were prioritized using microarray differential expressed genes. Of the top prioritized TLR 2 molecules, 70% were co-expressed. …”
Get full text

Performance and clinical implications of non-invasive prenatal testing for rare chromosomal abnormalities: a retrospective study of 94,125 cases by Haimei Qi, Haijun Chen, Zhuling Zhang, Jinhui Gan, Huifeng Liu, Xianping Yuan, Fuyu Luo, Junkun Chen, Shichun Shen

“…BackgroundNon-invasive prenatal testing (NIPT) has demonstrated robust performance in detecting common trisomies and copy number variations. …”
Get full text

Expression, clinical significance and biological function of CDC7 in osteosarcoma by CHEN Peijun, MAN Yunan, HE Mingwei, LIANG Bumin, HE Maolin

“…Methods The mRNA microarray data from the Gene Expression Omnibus (GEO) database was used to analyze the expression of CDC7 in OS tissues. …”
Get full text

Integrative Analysis of Clinical and Bioinformatics Databases to Reveal the Role of Peripheral Innate Immunity in Kawasaki Disease by Hongjun Ba, Lili Zhang, Huimin Peng, Jixun Lin, Youzhen Qin, Yao Wang

“…In this study, we analyzed public single-cell RNA sequencing (scRNA-seq) and microarray data of peripheral blood mononuclear cells from normal controls and KD patients. …”
Get full text

Transcription Analysis of the Myometrium of Labouring and Non-Labouring Women. by Gemma C Sharp, James L Hutchinson, Nanette Hibbert, Tom C Freeman, Philippa T K Saunders, Jane E Norman

“…Appropriate analysis of large microarray experiments that compare gene expression in non-labouring and labouring gestational tissues is necessary to help bridge these gaps in our knowledge. …”
Get full text

Acetylation suppresses breast cancer progression by sustaining CLYBL stability by Xinyue Deng, Chenglong Ma, Xingyu Chen, Ming Yi, Qianhua Cao, Ruocen Liao, Xingyu Lei, Longchang Bai, Bin Zhao, Yingnan Wang, Zhuoyang Shen, Liujing Wu, Chenfang Dong, Zhijun Dai

“…Methods CLYBL expression patterns in breast cancer were assessed using a breast cancer tissue microarray, immunohistochemistry, and publicly available datasets. …”
Get full text

Modulation of homologous recombination gene activity in breast tumor cells in an <i>in vitro</i> model by M. M. Tsyganov, A. A. Frolova, E. A. Kravtsova, I. A. Tsydenova, M. K. Ibragimova

“…To assess the presence of chromosomal aberrations, microarray analysis was performed on DNA chips.Results. …”
Get full text

Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation by Jiahui Dong, Ruiping Du, Yani Zhang, Bo Hong, Gengyun Sun

“…The expressions of CAV1 and EGFR in LUAD tissues and adjacent tissues were detected by Western blotting, immunohistochemistry and microarray technology. …”
Get full text

Molecular mechanism and biological pathway of high-expressed RAD51 in regulating cell adhesion and potentially affecting oral squamous cell carcinoma by Yu-Xing Tang, Yu-Yan Pang, Yan Cui, Guo-Jiao Wu, Lin-Qing Huang, Chang-Zhan Zhou, Bin Li, Qi Li, Bang-Teng Chi, Rong-Quan He, Di-Yuan Qin, Jian-Di Li, Gang Chen, Dong-Ming Li, Yi-Wu Dang

“…Methods Bulk RNA (RNA-seq and microarray), immunohistochemistry (IHC), and single-cell sequencing (scRNA-seq) were used to assess RAD51 expression and its clinical relevance in OSCC. …”
Get full text

IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming by Anbo Gao, Juan Zou, Tian Zeng, Mei Qin, Xing Tang, Ting Yi, Guangming Song, Jie Zhong, Yuhuan Zeng, Wenchao Zhou, Qin Gao, Qunfeng Zhang, Juan Zhang, Yukun Li

“…Abstract Ovarian cancer (OC) is often detected at an advanced stage and has a high recurrence rate after surgery or chemotherapy. …”
Get full text

Integrative analysis of T cell-mediated tumor killing-related genes reveals KIF11 as a novel therapeutic target in esophageal squamous cell carcinoma by Xinxin Cheng, Huihui Zhao, Zhangwang Li, Liping Yan, Qingjie Min, Qingnan Wu, Qimin Zhan

“…The KIF11 expression was detected by multiplex fluorescent immunohistochemistry on tissue microarray from ESCC patients. …”
Get full text

Sequential prenatal diagnosis of fetal skeletal dysplasia: A cohort study by Mengting Jiang, Bin Zhang, Jing Wang, Wei Qiao, Xiuzhen Mao, Bin Yu

“…Abstract Introduction Genetic factors are considered to be the main factors leading to fetal skeletal dysplasia (SD), and chromosomal microarray analysis (CMA) has been used clinically for the detection of SD fetuses. …”
Get full text

Nanopore-based random genomic sampling for intraoperative molecular diagnosis by Francesco E. Emiliani, Abdol Aziz Ould Ismail, Edward G. Hughes, Gregory J. Tsongalis, George J. Zanazzi, Chun-Chieh Lin

“…Results In our retrospective cohort of 26 malignant brain tumors, iSCORED demonstrated 100% concordance in CNV detection, including chromosomal alterations and oncogene amplifications, when compared to clinically validated assays such as Next-Generation Sequencing and Chromosomal Microarray. …”
Get full text

Identification and Validation of a Potential Marker of Tissue Quality Using Gene Expression Analysis of Human Colorectal Tissue. by Nicole Lange, Florian T Unger, Monika Schöppler, Katja Pursche, Hartmut Juhl, Kerstin A David

“…Normal colorectal tissue and CRC tissue was analyzed using microarray and quantitative real-time PCR (qPCR). Comparing changes in gene expression between pre- and post-surgical tissue using microarray analysis identified a list of potential tissue quality biomarkers and this list was validated using qPCR. …”
Get full text

Renal tissue thawed for 30 minutes is still suitable for gene expression analysis. by Yi Ma, Xiao-Nan Kang, Wen-Bin Ding, Hao-Zheng Yang, Ye Wang, Jin Zhang, Yi-Ran Huang, Hui-Li Dai

“…We aim to evaluate the effects of thawing at room temperature for different time periods on gene expression analysis. …”
Get full text

AgileMultiIdeogram: Rapid Identification and Visualization of Autozygous Regions Using Illumina Short-Read Sequencing Data by Christopher M. Watson, Carolina Lascelles, Morag Raynor, Marilena Elpidorou, Ummey Hany, Laura Crinnion, Colin A. Johnson, Eamonn Sheridan, Alexander F. Markham, James A. Poulter, David T. Bonthron, Ian M. Carr

“…A well-established and effective way of identifying these “autozygous” genomic regions has been to search for runs of homozygous genotypes in microarray SNP data. …”
Get full text