On-Chip DNA Assembly via Dielectrophoresis by Xichuan Rui, Lin-Sheng Wu, Xin Zhao

“…This work introduces a novel on-chip DNA assembly method via dielectrophoresis (DEP) that can potentially be integrated with microarray-based oligo synthesis on the same chip. Our DEP chip can selectively manipulate oligos and guide their movement without perturbing the surrounding fluid medium, thus aiding in DNA assembly. …”
Get full text

Gene network inference and biochemical assessment delineates GPCR pathways and CREB targets in small intestinal neuroendocrine neoplasia. by Ignat Drozdov, Bernhard Svejda, Bjorn I Gustafsson, Shrikant Mane, Roswitha Pfragner, Mark Kidd, Irvin M Modlin

“…High throughput techniques such as inference of gene regulatory networks from microarray experiments can objectively define signaling machinery in this disease. …”
Get full text

A stratified transcriptomics analysis of polygenic fat and lean mouse adipose tissues identifies novel candidate obesity genes. by Nicholas M Morton, Yvonne B Nelson, Zoi Michailidou, Emma M Di Rollo, Lynne Ramage, Patrick W F Hadoke, Jonathan R Seckl, Lutz Bunger, Simon Horvat, Christopher J Kenyon, Donald R Dunbar

“…Selected candidate genes Npr3 and Thbs1, as well as Gys2, a non-QTL gene that otherwise passed our enrichment criteria were characterised, revealing novel functional effects consistent with a contribution to obesity.…”
Get full text

Translational profiling reveals novel gene expression changes in the direct and indirect pathways in a mouse model of levodopa induced dyskinesia by Sabika Jafri, Mahdi Ghani, Natalie Stickle, Carl Virtanen, Lili-Naz Hazrati, Naomi P. Visanji, Naomi P. Visanji, Naomi P. Visanji

“…Extracted ribosomal RNA was amplified, purified, and gene expression was quantified using microarray.ResultsOne hundred ninety-five significantly varying transcripts were identified among the four treatment groups. …”
Get full text

Characterization of transcriptional changes in ERG rearrangement-positive prostate cancer identifies the regulation of metabolic sensors such as neuropeptide Y. by Petra Massoner, Karl G Kugler, Karin Unterberger, Ruprecht Kuner, Laurin A J Mueller, Maria Fälth, Georg Schäfer, Christof Seifarth, Simone Ecker, Irmgard Verdorfer, Armin Graber, Holger Sültmann, Helmut Klocker

“…Functional analyses do not fully explain the selective pressure causing ERG rearrangement during the development of prostate cancer. …”
Get full text

Clustering, Pathway Enrichment, and Protein-Protein Interaction Analysis of Gene Expression in Neurodevelopmental Disorders by Ruchi Yadav, Prachi Srivastava

“…In this study, we have used four samples of microarray neuronal developmental data: control, RV (resveratrol), NGF (nerve growth factor), and RV + NGF. …”
Get full text

An Ensemble Classification Method for High-Dimensional Data Using Neighborhood Rough Set by Jing Zhang, Guang Lu, Jiaquan Li, Chuanwen Li

“…Mining useful knowledge from high-dimensional data is a hot research topic. Efficient and effective sample classification and feature selection are challenging tasks due to high dimensionality and small sample size of microarray data. …”
Get full text

A multi-omic meta-analysis reveals novel mechanisms of insecticide resistance in malaria vectors by Sanjay C. Nagi, Victoria A. Ingham

“…This study, employing a cross-species approach, integrates RNA-Sequencing, whole-genome sequencing, and microarray data to elucidate drivers of insecticide resistance in Anopheles gambiae complex and An. funestus. …”
Get full text

Two stable variants of Burkholderia pseudomallei strain MSHR5848 express broadly divergent in vitro phenotypes associated with their virulence differences. by A A Shea, R C Bernhards, C K Cote, C J Chase, J W Koehler, C P Klimko, J T Ladner, D A Rozak, M J Wolcott, D P Fetterer, S J Kern, G I Koroleva, S P Lovett, G F Palacios, R G Toothman, J A Bozue, P L Worsham, S L Welkos

“…Microscopic and colony morphology differences on six differential media were observed and only the Rough variant metabolized sugars in selective agar. Antimicrobial susceptibilities and lipopolysaccharide (LPS) features were characterized and phenotype microarray profiles revealed distinct metabolic and susceptibility disparities between the variants. …”
Get full text

HuoXueJieDu Formula Alleviates Diabetic Retinopathy in Rats by Inhibiting SOCS3-STAT3 and TIMP1-A2M Pathways by Hongliang Wang, Wei Xing, Shijie Tang, Zhenglin Wang, Tiantian Lv, Yan Wu, Shuzhen Guo, Chun Li, Jing Han, Ruixin Zhu, Wei Wang

“…Whole genome expression profiles in rat retinas were examined using microarray technology. Differential gene expression and pathway enrichment analysis were conducted on the microarray data, with validation through real-time PCR and immunohistochemical staining. …”
Get full text

Weighted frequent gene co-expression network mining to identify genes involved in genome stability. by Jie Zhang, Kewei Lu, Yang Xiang, Muhtadi Islam, Shweta Kotian, Zeina Kais, Cindy Lee, Mansi Arora, Hui-Wen Liu, Jeffrey D Parvin, Kun Huang

“…Gene co-expression network analysis is an effective method for predicting gene functions and disease biomarkers. …”
Get full text

The transcription factors Snail and Slug activate the transforming growth factor-beta signaling pathway in breast cancer. by Archana Dhasarathy, Dhiral Phadke, Deepak Mav, Ruchir R Shah, Paul A Wade

“…In order to obtain a global view of the impact of Snail and Slug expression, we performed a microarray experiment using the MCF-7 breast cancer cell line, which does not express detectable levels of Snail or Slug. …”
Get full text

Prevention of hypovolemic circulatory collapse by IL-6 activated Stat3. by Jeffrey A Alten, Ana Moran, Anna I Tsimelzon, Mary-Ann A Mastrangelo, Susan G Hilsenbeck, Valeria Poli, David J Tweardy

“…Pre-treatment of rats with a selective inhibitor of Stat3, T40214, reduced the IL-6-mediated increase in cardiac Stat3 activity, blocked successful resuscitation by IL-6 and reversed IL-6-mediated protection from cardiac apoptosis. …”
Get full text

Bioinformatics and network pharmacology-based study to elucidate the multi-target pharmacological mechanism of the indigenous plants of Medina valley in treating HCV-related hepato... by Mubarak A. Alamri

“…But the findings were not validated in actual patients, so further investigation is needed to confirm the potential use of selected medicinal plants towards HCV-related HC.…”
Get full text

Investigating Single-Molecule Molecular Inversion Probes for Medium-Scale Targeted DNA Methylation Analysis by Roy B. Simons, Hieab H. H. Adams, Manfred Kayser, Athina Vidaki

“…However, we still lack a cost-effective, sensitive, medium-scale method for the analysis of hundreds to thousands of user-defined CpGs suitable for minute DNA input amounts (<10 ng). …”
Get full text

Metabolomic characterisation of the glioblastoma invasive margin reveals a region-specific signature by James Wood, Stuart J. Smith, Marcos Castellanos-Uribe, Anbarasu Lourdusamy, Sean T. May, David A. Barrett, Richard G. Grundy, Dong-Hyun Kim, Ruman Rahman

“…Here, we use liquid chromatography–mass spectrometry and gene expression microarray to profile integrated intratumour metabolic heterogeneity, as a direct functional readout of adaptive responses of subclones to the tumour microenvironment. …”
Get full text

CD155 blockade enhances allogeneic natural killer cell-mediated antitumor response against osteosarcoma by Lei Shi, Christian M Capitini, Amy K Erbe, Fernanda Szewc, Longzhen Song, Matthew H Forsberg, David P Turicek, Paul D Bates, Devin M Burpee, Aicha E Quamine, Johnathan D Ebben, Jillian M Kline, Emily O Lafeber, Madison F Phillips, Monica M Cho, Amanda S Ceas, John A Kink

“…Background Allogeneic bone marrow transplant (alloBMT) is curative for hematologic malignancies through the graft-versus-tumor (GVT) effect but has been ineffective for solid tumors like osteosarcoma (OS). …”
Get full text

Maternal bisphenol a exposure impacts the fetal heart transcriptome. by Kalyan C Chapalamadugu, Catherine A Vandevoort, Matthew L Settles, Barrie D Robison, Gordon K Murdoch

“…At the end of treatment, fetal heart tissues were collected and chamber specific transcriptome expression was assessed using genome-wide microarray. Quantitative real-time PCR was conducted on select genes and ventricular tissue glycogen content was quantified. …”
Get full text

High prevalence of multidrug-tolerant bacteria and associated antimicrobial resistance genes isolated from ornamental fish and their carriage water. by David W Verner-Jeffreys, Timothy J Welch, Tamar Schwarz, Michelle J Pond, Martin J Woodward, Sarah J Haig, Georgina S E Rimmer, Edward Roberts, Victoria Morrison, Craig Baker-Austin

“…<h4>Methodology/principal findings</h4>To assess the potential effects of this sustained selection pressure, 127 Aeromonas spp. isolated from warm and cold water ornamental fish species were screened for tolerance to 34 antimicrobials. …”
Get full text

Diagnosis of Coronary Heart Diseases Using Gene Expression Profiling; Stable Coronary Artery Disease, Cardiac Ischemia with and without Myocardial Necrosis. by Nabila Kazmi, Tom R Gaunt

“…Interestingly it also showed efficacy in discriminating myocardial infarction patients from patients with clinical symptoms of cardiac ischemia but no myocardial necrosis or stable coronary artery disease, despite the influence of batch effects and different microarray gene chips and platforms.…”
Get full text