MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
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Caveolin-1 inhibits the proliferation and invasion of lung adenocarcinoma via EGFR degradation by Jiahui Dong, Ruiping Du, Yani Zhang, Bo Hong, Gengyun Sun

“…Cell apoptosis and cell cycle were detected by flow cytometry. The effect of CAV1 on EGFR degradation was verified by cycloheximide inhibition assay. …”
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Transcriptomic profiling of cytomegalovirus infection in cardiac transplantation: proof-of-concept for a new strategy in tissue markers application by Ilaria Barison, Diego Perazzolo, Chiara Castellani, Alessia Giarraputo, Elisabetta Rossi, Elisabetta Rossi, Luca Vedovelli, Sonia Anna Minuzzo, Chiara Tessari, Nicola Pradegan, Giuseppe Toscano, Francesco Tona, Cristina Basso, Gino Gerosa, Susanna Mandruzzato, Susanna Mandruzzato, Davide Abate, Dario Gregori, Annalisa Angelini, Marny Fedrigo

“…Based on the bioinformatic analysis, we explored the regulatory effects of these miRNAs on the mRNA pathways independently identified in the same samples. …”
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FGFR3-TACC3 fusion gene promotes glioblastoma malignant progression through the activation of STAT3 signaling pathway by Yiming Li, Yiming Li, Jianshen Liang, Jianshen Liang, Jianshen Liang, Xiude Ren, Xiude Ren, Jiahe Guo, Jiahe Guo, Jiahe Guo, Xisen Wang, Xisen Wang, Xisen Wang, Xuya Wang, Xuya Wang, Xuya Wang, Shengping Yu, Shengping Yu, Tao Li, Tao Li, Xuejun Yang, Xuejun Yang, Xuejun Yang

“…A series of phenotypic experiments, including CCK-8, EdU, colony-formation assay, wound healing assay, and transwell assay were conducted to detect the effects of F3-T3 on proliferation, invasion, and migration of glioma cells. …”
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Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis. by Claudia Bühnemann, Simon Li, Haiyue Yu, Harriet Branford White, Karl L Schäfer, Antonio Llombart-Bosch, Isidro Machado, Piero Picci, Pancras C W Hogendoorn, Nicholas A Athanasou, J Alison Noble, A Bassim Hassan

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”
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Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling by Nikole J. Byrne, Christoph Koentges, Elisabeth Khan, Katharina Pfeil, Robert Sandulescu, Sayan Bakshi, Carolin Költgen, Ivan Vosko, Johannes Gollmer, Thomas Rathner, Günter Roth, Michael M. Hoffmann, Katja E. Odening, Hauke Horstmann, Luke A. Potter, Christoph Bode, Dennis Wolf, Harald Sourij, Senka Ljubojevic-Holzer, Markus Wallner, Peter P. Rainer, Simon Sedej, Daniel Scherr, Dirk von Lewinski, Adam R. Wende, Andreas Zirlik, Heiko Bugger

“…To current study was designed to explore mechanisms underlying adverse effects of increased SIRT4 levels in the heart following pressure overload. …”
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WTAP/YTHDF1-mediated m6A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs by Quan Chen, Luoquan Ao, Qing Zhao, Lu Tang, Yanli Xiong, Yuchuan Yuan, Xiaofeng Wu, Wei Xing, Zhan Li, Wei Guo, Huaping Liang, Song Guo Zheng, Qizhou Lian, Di Lu, Weijun Wan, Xiang Xu

“…Methods: Epitranscriptomic microarray analysis and MeRIP-qPCR assay were performed to identify the regulatory effect of WTAP in the IFN-γ-licensing MSCs. …”
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In vitro methodologies to evaluate nanocarriers for cancer treatment: where are we? by Giovanna Cassone Salata, Camila Megumi Hirokawa, Giovanna Barros de Melo, Isabella Draszesski Malagó, Jessica Ribeiro Nunes, Julia Sapienza Passos, Regina Gomes Daré, Luciana B. Lopes

“…Abstract Cancer treatment continues to face significant challenges, including lack of selectivity, systemic toxicity, and the adaptive resistance mechanisms of cancer cells, which limit the effectiveness of conventional therapies. …”
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IL-6 is a prognostic biomarker in patients with advanced esophageal squamous cell carcinoma received with PD-1 inhibitors by Ping Huang, Manyi Zhao, Jianhong Xia, Hongliang Li, Junxia Sun, Xin Li, Chunsheng Yang, Guangyi Gao, Wenhang Zhou, Meifeng Zhong, Hongmei Yong

“…BackgroundDue to the low efficacy rates, effective biomarkers are desperately needed to determine populations of advanced esophageal squamous cell carcinoma (ESCC) that may benefit from immune checkpoint inhibitor (ICI) treatment.ObjectivesTo explore the relationship between IL-6 and the esophageal cancer tumor immune microenvironment using online databases and esophageal cancer tissue microarrays and to investigate the predictive role of IL-6 for immunotherapy in esophageal squamous carcinoma patients through clinical study data.MethodsRNA-seq datasets of ESCC patients were obtained from TCGA, and the relationship between IL-6 and immune cells was discovered using TIMER 2.0 databases. …”
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Rotterdam Oncology Documentation (RONCDOC) – a high-quality data warehouse and tissue collection for head and neck cancer by Arta Hoesseini, Emilie A. C. Dronkers, Eveline Dieleman, Maria J. De Herdt, Marjan. H. Wieringa, Marie-Louise F. van Velthuysen, Marinella P. J. Offerman, Aniel Sewnaik, Dominiek Monserez, Stijn Keereweer, Jose A. U. Hardillo, Robert Jan Baatenburg de Jong

“…In addition, hospital-based cohorts that include more variables are often not collected consecutively, resulting in selection bias. Therefore, we established a hospital-based cancer registry system “Rotterdam Oncology Documentation” (RONCDOC), a complete and consecutive data warehouse and tissue collection for HNC, directly registered at the source. …”
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Analysis of Result Variability from High-Density Oligonucleotide Arrays Comparing Same-Species and Cross-Species Hybridizations by J.D. Chismar, T. Mondala, H.S. Fox, E. Roberts, D. Langford, E. Masliah, D.R. Salomon, S.R. Head

“…There exists a significant limitation in the variety of organisms for which microarrays have been developed because of a lack of genomic sequence data. …”
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Decoding the prognostic landscape of LUAD: the interplay between N6-methyladenosine modification and immune microenvironment by Quan Chen, Quan Chen, Weijun Wan, Qing Zhao, Juan Li, Juan Li, Yanli Xiong, Yanli Xiong, Yuchuan Yuan, Lu Tang, Xiaofeng Wu, Wei Xing, Wei Guo, Di Lu, Luoquan Ao, Xiang Xu, Xiang Xu, Xiang Xu, Xiang Ao, Xiang Ao, Xiang Ao

“…A risk score model was constructed using least absolute shrinkage and selection operator regression and validated in two independent cohorts and LUAD tissue microarrays. …”
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Adrenocortical carcinoma survival gene HMMR was identified as being targeted by fluorouracil and epirubicin using a gene coexpression network-based drug repositioning strategy by Zahra Jafari, Seyed-Morteza Javadirad, Seyede Elmira Yazdi Rouholamini

“…Drug repositioning based on gene coexpression networks has proven to be an effective method of discovering potential cancer treatments. …”
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Development and In Vitro Characterization of [³H]GMC-058 as Radioligand for Imaging Parkinsonian-Related Proteinopathies by Andrea Varrone, Vasco C. Sousa, Manolo Mugnaini, Sandra Biesinger, Gunnar Nordvall, Lee Kingston, Ileana Guzzetti, Charles S. Elmore, Dan Sunnemark, Dinahlee Saturnino Guarino, Sjoerd J. Finnema, Magnus Schou

“…A library of 78 small molecules was developed and screened using recombinant α-syn fibrils and brain homogenates from Alzheimer’s disease (AD) donors. The selection criteria were as follows: <i>K</i>i_α-syn < 30 nM, <i>K</i>i_tau and <i>K</i>i_A-β > 200 nM. …”
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The antiprotease Spink7 promotes inflammation resolution by modulating multiple proteases activities during wound healing by Na Zhao, Guojian Wang, Shuang Long, Xiaofan Lv, Xinze Ran, Junping Wang, Yongping Su, Tao Wang

“…Abstract Background Effective control of inflammation is crucial for the healing of cutaneous wounds, but the molecular mechanisms governing inflammation resolution during wound closure are still not yet clear. …”
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Comprehensive analysis of the clinical and biological significances of polyamine metabolism in glioma by Fei Bai, Kexin Zhai, Tiantian Yao, Yuxia Zhou, Yongxiang Zhao, Guanghui Yang

“…What’s more, IHC staining was conducted on glioma tissue microarrays to confirm the expression patterns of genes related to polyamine metabolism in glioma tissues. …”
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The relative resistance of children to sepsis mortality: from pathways to drug candidates by Rose B Joachim, Gabriel M Altschuler, John N Hutchinson, Hector R Wong, Winston A Hide, Lester Kobzik

“…To find drugs that could promote beneficial (child) pathways or inhibit harmful (adult) ones, we built an in silico pathway drug network (PDN) using expression correlation between drug, disease, and pathway gene signatures across 58,475 microarrays. Specific pathway clusters from children or adults were assessed for correlation with drug‐based signatures. …”
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Integrated analyses of copy number variations and gene expression in lung adenocarcinoma. by Tzu-Pin Lu, Liang-Chuan Lai, Mong-Hsun Tsai, Pei-Chun Chen, Chung-Ping Hsu, Jang-Ming Lee, Chuhsing Kate Hsiao, Eric Y Chuang

“…Identification of prognostic biomarkers for lung cancer using gene expression microarrays poses a major challenge in that very few overlapping genes have been reported among different studies. …”
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From Insect to Man: Photorhabdus Sheds Light on the Emergence of Human Pathogenicity. by Geraldine Mulley, Michael L Beeton, Paul Wilkinson, Isabella Vlisidou, Nina Ockendon-Powell, Alexia Hapeshi, Nick J Tobias, Friederike I Nollmann, Helge B Bode, Jean van den Elsen, Richard H ffrench-Constant, Nicholas R Waterfield

“…Photorhabdus are highly effective insect pathogenic bacteria that exist in a mutualistic relationship with Heterorhabditid nematodes. …”
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Diagnosing Autoimmune Bullous Diseases—An Indian Perspective by Adhyatm Bhandari, Dipankar De, Shikha Shah, Debajyoti Chatterjee, Vinod Kumar, Rahul Mahajan, Sanjeev Handa

“…Accurate diagnosis of the specific subtype of AIBD is crucial for effective management and predicting prognosis, especially in cases with an increased risk of malignancy. …”
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