A cross-tissue transcriptomic approach decodes glucocorticoid receptor-dependent links to human metabolic phenotypes by Marcin Piechota, Mateusz Zieba, Małgorzata Borczyk, Slawomir Golda, Jacek Hajto, Urszula Skupio, Michal Slezak, Jan Rodriguez Parkitna, Michal Korostynski

“…Here, we examine cross-tissue effects of GR activation on gene expression. We assessed changes induced by stimulation with GR agonist, dexamethasone in nine tissues (adrenal cortex, perigonadal adipose tissue, hypothalamus, liver, kidney, anterior thigh muscle, pituitary gland, spleen, and lungs) in adult male C57BL/6 mice, using whole-genome microarrays. …”
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Quantifying intratumoral biomarker heterogeneity in tubo-ovarian high-grade serous carcinoma to optimize clinical translation by Aline Talhouk, Derek S. Chiu, Liliane Meunier, Kurosh Rahimi, Cécile Le Page, Monique Bernard, Diane Provencher, David G. Huntsman, Anne Marie Mes Masson, Martin Köbel

“…Markers were stained on tissue microarrays and scored using a continuous or categorical interpretation of staining patterns. …”
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PPY‐Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer by Mengdie Cao, Wang Peng, Bin Cheng, Ronghua Wang, Wei Chen, Luyao Liu, Hai Huang, Shiru Chen, Haochen Cui, JingWen Liang, Qiaodan Zhou, Si Xiong, Shuya Bai, Luoxia Liu, Yuchong Zhao

“…Unlike previously reported iCAF inducers, PPY is a gastrointestinal hormone predominantly expressed in the pancreas, suggesting that targeting it may have minimal systemic effects. Multiplex immunohistochemistry (mIHC) on human PDAC tissue microarrays, orthotopic allograft mouse models, and co‐culture experiments are utilized to validate the crucial role of PPY in iCAF induction. …”
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TPX2 promotes papillary renal cell carcinoma progression by forming a ceRNA with LINC00894 by Zhenshan Ding, Wenwei Ying, Ye Yan, Ying Zhao, Cheng Liu, Lulin Ma

“…Immunohistochemical analysis of tissue microarrays was performed to evaluate the associations between TPX2 expression and clinicopathological characteristics in type 2 pRCC patients. …”
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Malate initiates a proton-sensing pathway essential for pH regulation of inflammation by Yu-jia-nan Chen, Rong-chen Shi, Yuan-cai Xiang, Li Fan, Hong Tang, Gang He, Mei Zhou, Xin-zhe Feng, Jin-dong Tan, Pan Huang, Xiao Ye, Kun Zhao, Wen-yu Fu, Liu-li Li, Xu-ting Bian, Huan Chen, Feng Wang, Teng Wang, Chen-ke Zhang, Bing-hua Zhou, Wan Chen, Tao-tao Liang, Jing-tong Lv, Xia Kang, You-xing Shi, Ellen Kim, Yin-hua Qin, Aubryanna Hettinghouse, Kai-di Wang, Xiang-li Zhao, Ming-yu Yang, Yu-zhen Tang, Hai-long Piao, Lin Guo, Chuan-ju Liu, Hong-ming Miao, Kang-lai Tang

“…Here, we found that L-malate exerts anti-inflammatory effect via BiP-IRF2BP2 signaling, which is a sensor of cytosolic pH in macrophages. …”
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Thrombospondin-1 induces CD8+ T cell exhaustion and immune suppression within the tumor microenvironment of ovarian cancer by Haiyan Liang, Suwei Zhang

“…Methods We analyzed THBS1 expression and its clinical significance using publicly available datasets (TCGA-OV, GSE14407) and tissue microarrays containing OC and adjacent normal tissues. …”
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Increased COX-2 Immunostaining in Urothelial Carcinoma of the Urinary Bladder Is Associated with Invasiveness and Poor Prognosis by Basim Al-Maghrabi, Wafaey Gomaa, Mohammed Abdelwahed, Jaudah Al-Maghrabi

“…UCB samples were collected before any local or systemic therapy. Tissue microarrays were designed and constructed, and TMA blocks were sliced for further immunohistochemical staining. …”
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SUMOYLATION AS AN EARLY ADAPTIVE MECHANISM IN THE PROTEOMIC RESPONSE OF HUMAN GERM CELLS TO SIMULATED MICROGRAVITY

“…This study analyses the effects of simulated microgravity (SμG) and hypogravity (ShG) on different pathways including SUMOylation (Small Ubiquitin-like Modifier) in human male germ cell line TCam-2, combining Reverse-Phase Protein microArrays (RPPA) and immunofluorescence. …”
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Prenatal tobacco smoke exposure is associated with childhood DNA CpG methylation. by Carrie V Breton, Kimberly D Siegmund, Bonnie R Joubert, Xinhui Wang, Weiliang Qui, Vincent Carey, Wenche Nystad, Siri E Håberg, Carole Ober, Dan Nicolae, Kathleen C Barnes, Fernando Martinez, Andy Liu, Robert Lemanske, Robert Strunk, Scott Weiss, Stephanie London, Frank Gilliland, Benjamin Raby, Asthma BRIDGE consortium

“…Some of the detrimental effects may be due to epigenetic modifications, although few studies have investigated this hypothesis in detail.…”
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Expression patterns of MCM8 in lung adenocarcinoma and its correlation with key biological processes by Xu-Sheng Liu, Jin Xie, Rui-Min Wu, Gao-Chun Xiao, Yu Zhang, Zhi-Jun Pei

“…The mechanism underlying the effect of MCM8 on cancer development and the immune response remains to be elucidated.…”
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DJ-1 as a potential biomarker for the early diagnosis in lung cancer patients by Binbin Han, Jiwen Wang, Jia Gao, Shana Feng, Yu Zhu, Xuexiang Li, Ting Xiao, Jun Qi, Wei Cui

“…DJ-1 appears to have its greatest effect on tumourigenesis, and it may have a greater impact on early-stage lung cancers. …”
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Whole stromal fibroblast signature is linked to specific chemokine and immune infiltration patterns and to improved survival in NSCLC by Stefan Koeck, Arno Amann, Johan Kern, Marit Zwierzina, Edith Lorenz, Sieghart Sopper, Heinz Zwierzina, Finn Mildner, Martina Sykora, Susanne Sprung, Hubert Hackl, Florian Augustin, Hubert T. Maier, Andreas Pircher, Georg Pall, Dominik Wolf, Gabriele Gamerith

“…Immune cell infiltration was assessed via flow cytometry, chemokines were analyzed by immunoassays and RNA microarrays. Results were confirmed in specimens from NSCLC patients by flow cytometry or immunohistochemistry as well as in the TCGA data set. …”
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TMEM101 expression and its impact on immune cell infiltration and prognosis in hepatocellular carcinoma by Lingyun Kuang, Yilin Pang, Quangang Fang

“…Additionally, differences in TMEM101 expression in HCC cell lines and HCC tissue microarrays were examined using RT-qPCR, western blotting, and in situ hybridization staining. …”
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Regulation of Proteome Maintenance Gene Expression by Activators of Peroxisome Proliferator-Activated Receptor α by Hongzu Ren, Beena Vallanat, Holly M. Brown-Borg, Richard Currie, J. Christopher Corton

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ESR1 is co-expressed with closely adjacent uncharacterised genes spanning a breast cancer susceptibility locus at 6q25.1. by Anita K Dunbier, Helen Anderson, Zara Ghazoui, Elena Lopez-Knowles, Sunil Pancholi, Ricardo Ribas, Suzanne Drury, Kally Sidhu, Alexandra Leary, Lesley-Ann Martin, Mitch Dowsett

“…Our observations suggest that some of the biological effects previously attributed to ER could be mediated and/or modified by these co-expressed genes. …”
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The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway by Yuexi He, Xiran Yin, Jianjun Yan, Xue Li, Qing Sun

“…Methods. miRNA and lncRNA microarrays were performed using IL-22-induced HaCaT cells and psoriatic lesions, respectively. …”
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LncRNA MBNL1‐AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/PHLPP2/FOXO1 axis in bladder cancer by Xiaosong Wei, Xiaoming Yang, Beibei Wang, Yang Yang, Zhiwei Fang, Chengzhi Yi, Lei Shi, Dongkui Song

“…Abstract LncRNAs have been shown to play essential roles in bladder cancer (BC) progress. Our microarrays of clinical samples firstly screened that lncRNA muscleblind‐like 1 antisense RNA 1 (MBNL1‐AS1) was poorly expressed in BC tissues. …”
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CPT1A/HIF-1α positive feedback loop induced fatty acid oxidation metabolic pathway contributes to the L-ascorbic acid-driven angiogenesis in breast cancer by Xiao Ma, Baojian Zhang, Xuezhe Yin, ShiPeng Yang, Zhenhua Lin, Yang Yang, Xianchun Zhou

“…Methods First, cell functional assays and animal models were employed to elucidate the pro-carcinogenic effects of CPT1A on BC and its role in metabolic alterations. …”
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Associations of circulating insulin-like growth factor-1 and insulin-like growth factor binding protein-3 with the expression of stem cell markers in benign breast tissue by Lusine Yaghjyan, Yujing J. Heng, Brian R. Sardella, Divya Murthy, Matt B. Mahoney, Bernard Rosner, Kornelia Polyak, Maisey Ratcliff, Rulla M. Tamimi

“…Immunohistochemistry (IHC) was done on tissue microarrays. For each core, the IHC expression was assessed using QuPath, and expressed as % of cells that stain positively for a specific marker out of the total cell count. …”
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Genome-wide DNA methylation and gene expression in human placentas derived from assisted reproductive technology by Pauliina Auvinen, Jussi Vehviläinen, Karita Rämö, Ida Laukkanen, Heidi Marjonen-Lindblad, Essi Wallén, Viveca Söderström-Anttila, Hanna Kahila, Christel Hydén-Granskog, Timo Tuuri, Aila Tiitinen, Nina Kaminen-Ahola

“…Methods We performed genome-wide DNA methylation (EPIC Illumina microarrays) and gene expression (mRNA sequencing) analyses for a total of 80 ART and 77 control placentas. …”
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