Hedgehog Signaling Inhibition by Smoothened Antagonist BMS-833923 Reduces Osteoblast Differentiation and Ectopic Bone Formation of Human Skeletal (Mesenchymal) Stem Cells by Nihal AlMuraikhi, Nuha Almasoud, Sarah Binhamdan, Ghaydaa Younis, Dalia Ali, Muthurangan Manikandan, Radhakrishnan Vishnubalaji, Muhammad Atteya, Abdulaziz Siyal, Musaad Alfayez, Abdullah Aldahmash, Moustapha Kassem, Nehad M. Alajez

“…Global gene expression profiling was performed using the Agilent® microarray platform. Effects on in vivo ectopic bone formation were assessed by implanting hMSC mixed with hydroxyapatite-tricalcium phosphate granules subcutaneously in 8-week-old female nude mice, and the amount of bone formed was assessed using quantitative histology. …”
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Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway by Yang Sheng Wu, Zhe Ming Li, Yi Tao Chen, Shi Jie Dai, Xiao Jie Zhou, Yuan Xiao Yang, Jian Shu Lou, Li Ting Ji, Yu Ting Bao, Ling Xuan, Lu Ning Lin, Chang Yu Li

“…Berberine (BBR), a natural compound extracted from a Chinese herb, has been shown to effectively attenuate insulin resistance (IR) and inflammation in the clinic. …”
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GATA6 immunohistochemistry and prognosis after surgical resection of pancreatic adenocarcinoma: results from the ESPAC-4 trial by R.C. Grant, K. Duan, R. Jackson, W. Greenhalf, E. Costello-Goldring, P. Ghaneh, C. Halloran, D. Palmer, T. Hackert, M. Büchler, S. Hutchinson, S. Ramotar, A. Dodd, J. Wilson, F. Notta, G. O’Kane, J. Knox, J. Neoptolemos, S. Gallinger, S.E. Fischer

“…Two pathologists independently assessed GATA6 protein expression by immunohistochemistry in tissue microarray cores, manually and with digital assistance. …”
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Oncolytic adenovirus decreases the proportion of TIM-3+ subset of tumor-infiltrating CD8+ T cells with correlation to improved survival in patients with cancer by Matti Kankainen, Timo Joensuu, Akseli Hemminki, Juuso Juhila, Siri Tähtinen, Otto Hemminki, Anna Kanerva, Kristian Taipale, Ilkka Liikanen, Minna Oksanen, Dafne C A Quixabeira, Saru Basnet

“…Meanwhile, less is known about their effects on immune checkpoints, the main targets for passive immunotherapy of cancer. …”
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Differential expression pattern of CC chemokine receptor 7 guides precision treatment of hepatocellular carcinoma by Jie Qin, Qianyi Gong, Cheng Zhou, Jietian Xu, Yifei Cheng, Weiyue Xu, Di Zhu, Yiming Liu, Yuye Zhang, Yanru Wang, Lingling Gao, Lanfang Li, Wulei Hou, Qian Li, Binbin Liu, Yazhen Zhu, Zuoyun Wang, Jieyi Shi, Shuangjian Qiu, Chunmin Liang

“…In this study, the complex tumor microenvironment was identified by using tissue microarray (TMA), spatial transcriptomes and single-cell sequencing. …”
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Decoding chromosomal instability insights in CRC by integrating omics and patient-derived organoids by Federica Papaccio, Manuel Cabeza-Segura, Blanca García-Micó, Francisco Gimeno-Valiente, Sheila Zúñiga-Trejos, Valentina Gambardella, María Fernanda Gutiérrez‐Bravo, Carolina Martinez‐Ciarpaglini, Pilar Rentero‐Garrido, Tania Fleitas, Susana Roselló, Juan Antonio Carbonell-Asins, Marisol Huerta, David Moro-Valdezate, Desamparados Roda, Noelia Tarazona, Manuel M. Sánchez del Pino, Andrés Cervantes, Josefa Castillo

“…From a clinical perspective, a better knowledge of these tumour’s biology will help to guide therapeutic strategies more effectively. Methods We used high-density chromosomal microarray analysis to evaluate CIN level of patient-derived organoids (PDOs) and their original mCRC tissues. …”
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Variations in salivary microbiome and metabolites are associated with immunotherapy efficacy in patients with advanced NSCLC by DanHui Huang, YueHua Chen, Cui Li, Shuang Yang, LiShan Lin, XiaoNan Zhang, XiaoFang Su, LaiYu Liu, Haijin Zhao, Tingyue Luo, Shaoxi Cai, QianNan Ren, Hangming Dong

“…Besides, in vitro experiments and tumor tissue microarray, including 70 NSCLC patients, were utilized to further explore the potential mechanism linking the oral microbiome and immunotherapy efficacy. …”
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LAPTM4B enhances the stemness of CD133+ liver cancer stem-like cells via WNT/β-catenin signaling by Jiahong Wang, Jianping Liao, Ye Cheng, Meirong Chen, Aimin Huang

“…Therefore, we investigated the effects of LAPTM4B on the stemness regulation of cluster of differentiation 133 (CD133)+ liver cancer stem-like cells (CSLCs). …”
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Aldo-keto reductase family 1 member B10 prevents esophageal squamous cell carcinoma from reactive carbonyl species-induced cell death and promotes its progression by Shau-Hsuan Li, Wan-Ting Huang, Yen-Hao Chen, Hung-I Lu, Chien-Ming Lo, Hsin-Ting Tsai, Chang-Han Chen

“…Furthermore, the identified signaling pathway and its biological effects were investigated in ESCC cell lines in vitro and 4-nitroquinoline 1-oxide (4-NQO)-induced-ESCC murine model in vivo. …”
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Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis. by Claudia Bühnemann, Simon Li, Haiyue Yu, Harriet Branford White, Karl L Schäfer, Antonio Llombart-Bosch, Isidro Machado, Piero Picci, Pancras C W Hogendoorn, Nicholas A Athanasou, J Alison Noble, A Bassim Hassan

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”
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Midline 1 associated with Fas signaling enhances murine antigen-induced arthritis by Nina Lukač, Nina Lukač, Darja Flegar, Darja Flegar, Sara Priselac, Tomislav Kelava, Tomislav Kelava, Alan Šućur, Alan Šućur, Maša Filipović, Maša Filipović, Dino Šisl, Dino Šisl, Martina Fadljević, Igor Radanović, Vedran Katavić, Vedran Katavić, Nives Zimmermann, Danka Grčević, Danka Grčević, Nataša Kovačić, Nataša Kovačić

“…Transcriptome of sorted CD11b+Gr-1+ cells was analyzed by microarray, and effects of potential molecular driver Midline-1 (Mid-1) were analyzed in vitro and using Mid1 −/− mice.ResultsAmeliorated antigen-induced arthritis in Fas −/− mice is characterized by the lack of synovial accumulation of myeloid CD11b+Gr-1+ cells. …”
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Cellular interactions and Ion channel signatures in atrial fibrillation remodeling: insights from single-cell analysis and machine learning by Bin He, Yan Cheng, Juan Wang, Ya Zhan, YanQun Liu

“…Ion channel-related genes were extracted from microarray datasets and analyzed for differential expression and functional relevance to AF pathology. …”
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Metabolic‐Immune Suppression Mediated by the SIRT1‐CX3CL1 Axis Induces Functional Enhancement of Regulatory T Cells in Colorectal Carcinoma by Ruiyang Zi, Xiang Zhao, Limei Liu, Yijie Wang, Rui Zhang, Zhiheng Bian, Haoran Jiang, Taorui Liu, Yixin Sun, Han Peng, Xuesong Wang, Fanghao Lu, Chao Zhang, Fan Zhang, Qing Qin, Houjie Liang, Jianjun Li, Zhihao Wei, Yan Dong

“…Multiplex immunofluorescence (mIHC) analysis of a CRC tissue microarray confirmed the promoting effect of CX3CL1 on Treg infiltration. …”
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Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis by Chang Liu, Lei Shi, Zijun Meng, Manlin Zhang, Zhiqi Zhang, Yunzhe Li, Kaiwen Du, Muyao Yang, Lin Qiu, Jing Feng, Yuchen He, Jiayun Liu, Hua Zhang, Hongbin Zhang, Tingyuan Lang, Zhuo Yang

“…Combined tandem mass tag- and tissue microarray-based proteomic studies revealed YWHAB down-regulation in post-neoadjuvant chemotherapy OCPM tissues, which was confirmed in no-neoadjuvant-chemotherapy-response tissues, isolated OCPM stem cells, and induced cisplatin-resistant cells. …”
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Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5’tRFAla/DVL/β-catenin pathway inhibition by Zhipeng Zhang, Yanmei Wu, Wenqiang Liang, Zhifang Liao, Hongbo Liao, Xingxing Xing, Wenxin Yi, Zixuan Liu, Yicheng Li, Mengya Shi, Dongling Lin, Ting Gu, Biao Wu, Mingzhi Zou, Huilai Miao, Xin Wu

“…Abstract Although tsRNA has been demonstrated to modulate various physiological processes analogous to miRNA, the potential regulatory functions and mechanisms of tsRNAs related to the pharmacological effects of small molecule drugs remain unclear. Herein, it is shown that eurycomalactone (ELT), a natural product, can reversibly switch hepatocellular carcinoma (HCC) PLC/PRF/5 and HUH7 cells into a quiescent state. …”
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Interferon-stimulated circHOMER1 attenuates antiviral innate immunity by Ao Zhang, Ranran Yao, Shijin Geng, Haiying Wang, Rong-Chun Tang, Hengxiang Yu, Rui Xu, Hong-Yan Chen, Yunxuan Zhou, Lan Zhang, Jun Zhang

“…Notably, the concordant immunosuppressive effects of circHOMER1 were observed in mouse infection models. …”
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Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson’s disease progression by targeting DCLK1 by Li Liu, Xiangting Liu, Runze Zhang, Xiao Sun, Keping Zhang, Beibei Zheng, Ruoxi Yang, Kaiyue Yang, Guohua Song, Zhaoqiang Zhang

“…Levels of miRNA in exos were analyzed by gene expression profile microarray. Levels of miR-150-5p in exos and substantia nigra pars compacta (SNpc) were further confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
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E2F2(E2F transcription factor 2) as a potential therapeutic target in meibomian gland carcinoma: evidence from functional and epigenetic studies by Wei Wang, Hetong Wang, Xun Liu, Fei Xu, Qin Tang, Chuanli Zhang, Jiaqi Lin, Limin Zhu, Tingting Lin

“…Tissue samples from 3 normal meibomian glands (MG) and 36 MGC patients were used to construct a tissue microarray. Functional assays were performed by modifying E2F2 expression, including CCK8, wound healing, Transwell, and analysis of epithelial-mesenchymal transition (EMT)-related markers. …”
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Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy by Li-Min Lei, Fu-Xing-Zi Li, Xiao Lin, Feng Xu, Su-Kang Shan, Bei Guo, Ming-Hui Zheng, Ke-Xin Tang, Yi Wang, Qiu-Shuang Xu, Wen-Lu Ouyang, Jia-Yue Duan, Yun-Yun Wu, Ye-Chi Cao, Zhi-Ang Zhou, Si-Yang He, Yan-Lin Wu, Xi Chen, Zheng-Jun Lin, Yi Pan, Ling-Qing Yuan, Zhi-Hong Li

“…This study identifies that CT-EXO mediates CT-induced osteoporotic effects through miR-25-3p by inhibiting autophagy via targeting SATB2, presenting a novel mechanism underlying the effect of cold temperature on bone mass.…”
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GDF11 protects against mitochondrial-dysfunction-dependent NLRP3 inflammasome activation to attenuate osteoarthritis by Pengfei Zhang, Haoxin Zhai, Shuai Zhang, Xiaojie Ma, Ao Gong, Zhaoning Xu, Wei Zhao, Hui Song, Shufeng Li, Tengfei Zheng, Zhendong Ying, Lei Cheng, Yunpeng Zhao, Lei Zhang

“…In an in vivo surgically induced mouse model, intraarticular administration of recombinant human GDF11 alleviated OA pathogenesis, whereas GDF11 conditional knockout reversed this effect. Additionally, findings from the NLRP3-knockout DMM mouse model revealed that GDF11 exerted its protective effect by inhibiting NLRP3. …”
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