Novel Hypomorphic Mutation in FANCD2 Gene Observed in a Fetus with Multiple Congenital Anomalies by Radoslava Vazharova, Svetlana Vragaleva, Violeta Dimitrova, Samuil Ivanov, Lubomir Balabanski, Maxim Malinov, Draga Toncheva

“…The case illustrates that patients/families affected by rare monogenic disorders may benefit from application of modern technologies like microarrays and NGS.…”
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WTAP/YTHDF1-mediated m6A modification amplifies IFN-γ-induced immunosuppressive properties of human MSCs by Quan Chen, Luoquan Ao, Qing Zhao, Lu Tang, Yanli Xiong, Yuchuan Yuan, Xiaofeng Wu, Wei Xing, Zhan Li, Wei Guo, Huaping Liang, Song Guo Zheng, Qizhou Lian, Di Lu, Weijun Wan, Xiang Xu

“…Methods: Epitranscriptomic microarray analysis and MeRIP-qPCR assay were performed to identify the regulatory effect of WTAP in the IFN-γ-licensing MSCs. …”
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Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis. by Claudia Bühnemann, Simon Li, Haiyue Yu, Harriet Branford White, Karl L Schäfer, Antonio Llombart-Bosch, Isidro Machado, Piero Picci, Pancras C W Hogendoorn, Nicholas A Athanasou, J Alison Noble, A Bassim Hassan

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”
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Development and In Vitro Characterization of [³H]GMC-058 as Radioligand for Imaging Parkinsonian-Related Proteinopathies by Andrea Varrone, Vasco C. Sousa, Manolo Mugnaini, Sandra Biesinger, Gunnar Nordvall, Lee Kingston, Ileana Guzzetti, Charles S. Elmore, Dan Sunnemark, Dinahlee Saturnino Guarino, Sjoerd J. Finnema, Magnus Schou

“…[³H]GMC-058 was the only compound with negligible binding in controls, and was further evaluated using tissue microarrays, autoradiography on fresh-frozen brain slices, and in vitro saturation binding assay on brain homogenates. …”
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Microsatellite Instability and Loss of Heterozygosity in CCND1 Microsatellite loci at Chr.11q13 in OSCC Patients with Therapeutic Validation by Seema Gupta, Shally Batham, Biswajit Maity

“…Molecular laboratory techniques like microarrays, microsatellites, and single-nucleotide polymorphisms (SNPs) have created new opportunities for diagnosing diseases, identifying chromosomal aberrations, and detecting point mutations for medical verification. …”
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MMP14 and DDR2 are potential molecular markers for metastatic triple-negative breast cancer by Rabeah Al-Temaimi, Rasheed Ahmad, Fahd Al-Mulla

“…Here, we utilized TNBC tissue microarrays to determine the protein expression of seven matrix metalloproteinases (MMPs) and Discoidin domain receptor 2 (DDR2) in metastatic and non-metastatic TNBC to identify potential targets for immunotherapy. …”
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A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. by Davide Danovi, Amos Folarin, Sabine Gogolok, Christine Ender, Ahmed M O Elbatsh, Pär G Engström, Stefan H Stricker, Sladjana Gagrica, Ana Georgian, Ding Yu, Kin Pong U, Kevin J Harvey, Patrizia Ferretti, Patrick J Paddison, Jane E Preston, N Joan Abbott, Paul Bertone, Austin Smith, Steven M Pollard

“…We used live-cell imaging and high content image analysis tools and identified JNJ-10198409 (J101) as an agent that induces mitotic arrest at prometaphase in GNS cells but not NS cells. Antibody microarrays and kinase profiling suggested that J101 responses are triggered by suppression of the active phosphorylated form of polo-like kinase 1 (Plk1) (phospho T210), with resultant spindle defects and arrest at prometaphase. …”
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C20orf27 promotes hepatocellular carcinoma progression via NT5E by Yu He, Wen Zhang, Ming Chang, Yuanjie Chen, Risheng Lin, Xinyu Ye, Wei Mao, Yubin Lu, Yi Lu, Erbao Chen

“…Immunohistochemistry analysis of tissue microarrays suggested that NT5E expression was significantly associated with C20orf27 levels. …”
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IL-6 is a prognostic biomarker in patients with advanced esophageal squamous cell carcinoma received with PD-1 inhibitors by Ping Huang, Manyi Zhao, Jianhong Xia, Hongliang Li, Junxia Sun, Xin Li, Chunsheng Yang, Guangyi Gao, Wenhang Zhou, Meifeng Zhong, Hongmei Yong

“…BackgroundDue to the low efficacy rates, effective biomarkers are desperately needed to determine populations of advanced esophageal squamous cell carcinoma (ESCC) that may benefit from immune checkpoint inhibitor (ICI) treatment.ObjectivesTo explore the relationship between IL-6 and the esophageal cancer tumor immune microenvironment using online databases and esophageal cancer tissue microarrays and to investigate the predictive role of IL-6 for immunotherapy in esophageal squamous carcinoma patients through clinical study data.MethodsRNA-seq datasets of ESCC patients were obtained from TCGA, and the relationship between IL-6 and immune cells was discovered using TIMER 2.0 databases. …”
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Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers by Joviana Farhat, Lara Alzyoud, Mohammad AlWahsh, Animesh Acharjee, Basem Al‐Omari

“…Results In practice, fluorescence in situ hybridization, karyotype, microarrays and other genetic tests are mainly applied to identify specific genetic alterations and mutations associated with cancer progression. …”
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Evaluation of the diagnostic concordance of FDA-approved PD-L1 assays in clear cell renal cell carcinoma by Yoko Koh, Taigo Kato, Gaku Yamamichi, Eisuke Tomiyama, Makoto Matsushita, Koji Hatano, Atsunari Kawashima, Norio Nonomura, Motohide Uemura

“…In this study, we constructed tissue microarrays from 286 clear cell renal cell carcinoma (ccRCC) tissue samples and evaluated PD-L1 expression using the 22C3, 28 − 8, SP142, and SP263 PD-L1 assays. …”
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HERV activation segregates ME/CFS from fibromyalgia while defining a novel nosologic entity by Karen Giménez-Orenga, Eva Martín-Martínez, Lubov Nathanson, Elisa Oltra

“…By using HERV-V3 high-density microarrays (including over 350k HERV elements and more than 1500 immune-related genes) to interrogate the transcriptomes of peripheral blood mononuclear cells from female patients diagnosed with ME/CFS, FM, or both, and matched healthy controls (n = 43), this study fills this gap of knowledge. …”
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In vitro methodologies to evaluate nanocarriers for cancer treatment: where are we? by Giovanna Cassone Salata, Camila Megumi Hirokawa, Giovanna Barros de Melo, Isabella Draszesski Malagó, Jessica Ribeiro Nunes, Julia Sapienza Passos, Regina Gomes Daré, Luciana B. Lopes

“…Abstract Cancer treatment continues to face significant challenges, including lack of selectivity, systemic toxicity, and the adaptive resistance mechanisms of cancer cells, which limit the effectiveness of conventional therapies. …”
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Rotterdam Oncology Documentation (RONCDOC) – a high-quality data warehouse and tissue collection for head and neck cancer by Arta Hoesseini, Emilie A. C. Dronkers, Eveline Dieleman, Maria J. De Herdt, Marjan. H. Wieringa, Marie-Louise F. van Velthuysen, Marinella P. J. Offerman, Aniel Sewnaik, Dominiek Monserez, Stijn Keereweer, Jose A. U. Hardillo, Robert Jan Baatenburg de Jong

“…Furthermore, a comprehensive validation protocol was developed to guarantee the quality of the data. Tissue microarrays (TMAs) were constructed from resection specimens of patients with primary oral squamous cell carcinoma. …”
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Adrenocortical carcinoma survival gene HMMR was identified as being targeted by fluorouracil and epirubicin using a gene coexpression network-based drug repositioning strategy by Zahra Jafari, Seyed-Morteza Javadirad, Seyede Elmira Yazdi Rouholamini

“…Drug repositioning based on gene coexpression networks has proven to be an effective method of discovering potential cancer treatments. …”
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The antiprotease Spink7 promotes inflammation resolution by modulating multiple proteases activities during wound healing by Na Zhao, Guojian Wang, Shuang Long, Xiaofan Lv, Xinze Ran, Junping Wang, Yongping Su, Tao Wang

“…Abstract Background Effective control of inflammation is crucial for the healing of cutaneous wounds, but the molecular mechanisms governing inflammation resolution during wound closure are still not yet clear. …”
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Comprehensive analysis of the clinical and biological significances of polyamine metabolism in glioma by Fei Bai, Kexin Zhai, Tiantian Yao, Yuxia Zhou, Yongxiang Zhao, Guanghui Yang

“…What’s more, IHC staining was conducted on glioma tissue microarrays to confirm the expression patterns of genes related to polyamine metabolism in glioma tissues. …”
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Survivin Expression in Placentas with Intrauterine Growth Restriction by Pavo Perković, Sanja Štifter-Vretenar, Marina Perković, Marko Štefančić, Ena Holjević, Andrea Dekanić, Tea Štimac

“…<b>Methods</b>: Survivin presence was determined in 153 archival formalin-fixed and paraffin-embedded placental tissues from IUGR (N = 122) and uncomplicated (N = 31) term pregnancies. Tissue microarrays (TMAs) were constructed, and survivin expression was assessed using immunohistochemistry (IHC). …”
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The relative resistance of children to sepsis mortality: from pathways to drug candidates by Rose B Joachim, Gabriel M Altschuler, John N Hutchinson, Hector R Wong, Winston A Hide, Lester Kobzik

“…To find drugs that could promote beneficial (child) pathways or inhibit harmful (adult) ones, we built an in silico pathway drug network (PDN) using expression correlation between drug, disease, and pathway gene signatures across 58,475 microarrays. Specific pathway clusters from children or adults were assessed for correlation with drug‐based signatures. …”
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Decoding the prognostic landscape of LUAD: the interplay between N6-methyladenosine modification and immune microenvironment by Quan Chen, Quan Chen, Weijun Wan, Qing Zhao, Juan Li, Juan Li, Yanli Xiong, Yanli Xiong, Yuchuan Yuan, Lu Tang, Xiaofeng Wu, Wei Xing, Wei Guo, Di Lu, Luoquan Ao, Xiang Xu, Xiang Xu, Xiang Xu, Xiang Ao, Xiang Ao, Xiang Ao

“…A risk score model was constructed using least absolute shrinkage and selection operator regression and validated in two independent cohorts and LUAD tissue microarrays. …”
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