In vivo RNAi screen and validation reveals Ngp, Hba-a1, and S100a8 as novel inhibitory targets on T lymphocytes in liver cancer by Inga Hochnadel, Lisa Hoenicke, Nataliia Petriv, Huizhen Suo, Lothar Groebe, Chantal Olijnik, Nina Bondarenko, Nina Bondarenko, Juan C. Alfonso, Michael Jarek, Ruibing Shi, Andreas Jeron, Andreas Jeron, Kai Timrott, Tatjana Hirsch, Nils Jedicke, Dunja Bruder, Dunja Bruder, Frank Klawonn, Frank Klawonn, Frank Klawonn, Ralf Lichtinghagen, Robert Geffers, Henrike Lenzen, Henrike Lenzen, Michael P. Manns, Tetyana Yevsa

“…Therefore, in this study, we aimed to define novel ICI molecules arising on T cells during aggressive HCC development.MethodsUsing autochthonous HCC models, we performed microarray analyses followed by in vivo RNA interference screen and identified several new ICI molecules on CD4 and CD8 T lymphocytes in HCC-bearing mice. …”
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Molecular profiling analysis for colorectal cancer patients with Pi-Xu or Shi-Re syndrome by Xinbing Sui, Yong Guo, Wei Ni, Haiyan Jin, Haoming Lin, Tian Xie

“…The protein expression was determined by Immunohistochemistry (IHC) staining and mRNA profiling was detected by expression microarray. Furthermore, mRNA fold change was evaluated by qRT-PCR. …”
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Midline 1 associated with Fas signaling enhances murine antigen-induced arthritis by Nina Lukač, Nina Lukač, Darja Flegar, Darja Flegar, Sara Priselac, Tomislav Kelava, Tomislav Kelava, Alan Šućur, Alan Šućur, Maša Filipović, Maša Filipović, Dino Šisl, Dino Šisl, Martina Fadljević, Igor Radanović, Vedran Katavić, Vedran Katavić, Nives Zimmermann, Danka Grčević, Danka Grčević, Nataša Kovačić, Nataša Kovačić

“…Transcriptome of sorted CD11b+Gr-1+ cells was analyzed by microarray, and effects of potential molecular driver Midline-1 (Mid-1) were analyzed in vitro and using Mid1 −/− mice.ResultsAmeliorated antigen-induced arthritis in Fas −/− mice is characterized by the lack of synovial accumulation of myeloid CD11b+Gr-1+ cells. …”
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urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. by Stephen Watt, Juan Mata, Luis López-Maury, Samuel Marguerat, Gavin Burns, Jürg Bähler

“…<h4>Methodology/principal findings</h4>We used S. pombe microarrays to identify three neighbouring genes (urg1, urg2, and urg3) whose transcript levels rapidly and strongly increased in response to uracil, a condition which otherwise had little effect on global gene expression. …”
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Cellular interactions and Ion channel signatures in atrial fibrillation remodeling: insights from single-cell analysis and machine learning by Bin He, Yan Cheng, Juan Wang, Ya Zhan, YanQun Liu

“…Ion channel-related genes were extracted from microarray datasets and analyzed for differential expression and functional relevance to AF pathology. …”
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Metabolic‐Immune Suppression Mediated by the SIRT1‐CX3CL1 Axis Induces Functional Enhancement of Regulatory T Cells in Colorectal Carcinoma by Ruiyang Zi, Xiang Zhao, Limei Liu, Yijie Wang, Rui Zhang, Zhiheng Bian, Haoran Jiang, Taorui Liu, Yixin Sun, Han Peng, Xuesong Wang, Fanghao Lu, Chao Zhang, Fan Zhang, Qing Qin, Houjie Liang, Jianjun Li, Zhihao Wei, Yan Dong

“…Multiplex immunofluorescence (mIHC) analysis of a CRC tissue microarray confirmed the promoting effect of CX3CL1 on Treg infiltration. …”
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Restriction of YWHAB-mediated YAP cytoplasmic retention is a novel mechanism underlying stemness maintenance and chemoresistance in ovarian cancer peritoneal metastasis by Chang Liu, Lei Shi, Zijun Meng, Manlin Zhang, Zhiqi Zhang, Yunzhe Li, Kaiwen Du, Muyao Yang, Lin Qiu, Jing Feng, Yuchen He, Jiayun Liu, Hua Zhang, Hongbin Zhang, Tingyuan Lang, Zhuo Yang

“…Combined tandem mass tag- and tissue microarray-based proteomic studies revealed YWHAB down-regulation in post-neoadjuvant chemotherapy OCPM tissues, which was confirmed in no-neoadjuvant-chemotherapy-response tissues, isolated OCPM stem cells, and induced cisplatin-resistant cells. …”
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Chronic sleep deprivation induces plasma exosome-derived miR-150-5p downregulation as a novel mechanism involved in Parkinson’s disease progression by targeting DCLK1 by Li Liu, Xiangting Liu, Runze Zhang, Xiao Sun, Keping Zhang, Beibei Zheng, Ruoxi Yang, Kaiyue Yang, Guohua Song, Zhaoqiang Zhang

“…Levels of miRNA in exos were analyzed by gene expression profile microarray. Levels of miR-150-5p in exos and substantia nigra pars compacta (SNpc) were further confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR). …”
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E2F2(E2F transcription factor 2) as a potential therapeutic target in meibomian gland carcinoma: evidence from functional and epigenetic studies by Wei Wang, Hetong Wang, Xun Liu, Fei Xu, Qin Tang, Chuanli Zhang, Jiaqi Lin, Limin Zhu, Tingting Lin

“…Tissue samples from 3 normal meibomian glands (MG) and 36 MGC patients were used to construct a tissue microarray. Functional assays were performed by modifying E2F2 expression, including CCK8, wound healing, Transwell, and analysis of epithelial-mesenchymal transition (EMT)-related markers. …”
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A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response by Xiao‐Ming Lyu, Xiao‐Wei Zhu, Manli Zhao, Xian‐Bo Zuo, Zhong‐Xi Huang, Xiao Liu, Tao Jiang, Xue‐Xi Yang, Xin Li, Xiao‐Bing Long, Jian‐Guo Wang, Jin‐Bang Li, Ming‐Yu Han, Shuang Wang, Teng‐Fei Liu, Bo Zhang, Tao Sun, Zhi Cheng, Mo‐Chang Qiu, Lei Dong, Lu Zheng, Long‐Cheng Zhang, Jia‐Hong Wang, Gan‐Guan Wei, Kaitai Yao, Qian Wang, Hou‐Feng Zheng, Xin Li

“…Significance analysis of microarray suggested that TRIM26 downregulation was related to low immune response in NPC. …”
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Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy by Li-Min Lei, Fu-Xing-Zi Li, Xiao Lin, Feng Xu, Su-Kang Shan, Bei Guo, Ming-Hui Zheng, Ke-Xin Tang, Yi Wang, Qiu-Shuang Xu, Wen-Lu Ouyang, Jia-Yue Duan, Yun-Yun Wu, Ye-Chi Cao, Zhi-Ang Zhou, Si-Yang He, Yan-Lin Wu, Xi Chen, Zheng-Jun Lin, Yi Pan, Ling-Qing Yuan, Zhi-Hong Li

“…This study identifies that CT-EXO mediates CT-induced osteoporotic effects through miR-25-3p by inhibiting autophagy via targeting SATB2, presenting a novel mechanism underlying the effect of cold temperature on bone mass.…”
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GDF11 protects against mitochondrial-dysfunction-dependent NLRP3 inflammasome activation to attenuate osteoarthritis by Pengfei Zhang, Haoxin Zhai, Shuai Zhang, Xiaojie Ma, Ao Gong, Zhaoning Xu, Wei Zhao, Hui Song, Shufeng Li, Tengfei Zheng, Zhendong Ying, Lei Cheng, Yunpeng Zhao, Lei Zhang

“…In an in vivo surgically induced mouse model, intraarticular administration of recombinant human GDF11 alleviated OA pathogenesis, whereas GDF11 conditional knockout reversed this effect. Additionally, findings from the NLRP3-knockout DMM mouse model revealed that GDF11 exerted its protective effect by inhibiting NLRP3. …”
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Gingipains disrupt bone homeostasis via dual regulation of osteogenesis and osteoclastogenesis through exosomal miR-146a-5p/TRAF6 signaling by Jiachen Dong, Jiachen Dong, Jiachen Dong, Yue Liao, Yue Liao, Yue Liao, Mengjun Sun, Mengjun Sun, Mengjun Sun, Huiwen Chen, Huiwen Chen, Huiwen Chen, Kecong Zhou, Kecong Zhou, Kecong Zhou, Huanyu Zhang, Huanyu Zhang, Huanyu Zhang, Wei Zhou, Wei Zhou, Wei Zhou, Zhongchen Song, Zhongchen Song, Zhongchen Song

“…The effects of gingipains on bone marrow mesenchymal stem cells (BMSCs) and RAW264.7 macrophages were assessed using cell proliferation assays, qPCR, western blot, microarray analysis, and dual-luciferase reporter assays. …”
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A nomogram for predicting 3-year total weight loss percentage following LSG: insights from visceral adipose tissue inflammatory methylation sites by Zhehong Li, Liang Wang, Zheng Wang, Qiqige Wuyun, Buhe Amin, Dongbo Lian, Guangzhong Xu, Nengwei Zhang, Dezhong Wang

“…Comparative analyses of 850K methylation microarrays from VAT samples were performed to identify methylation sites associated with INF-related genes. …”
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Transcriptomic profiling of cytomegalovirus infection in cardiac transplantation: proof-of-concept for a new strategy in tissue markers application by Ilaria Barison, Diego Perazzolo, Chiara Castellani, Alessia Giarraputo, Elisabetta Rossi, Elisabetta Rossi, Luca Vedovelli, Sonia Anna Minuzzo, Chiara Tessari, Nicola Pradegan, Giuseppe Toscano, Francesco Tona, Cristina Basso, Gino Gerosa, Susanna Mandruzzato, Susanna Mandruzzato, Davide Abate, Dario Gregori, Annalisa Angelini, Marny Fedrigo

“…Based on the bioinformatic analysis, we explored the regulatory effects of these miRNAs on the mRNA pathways independently identified in the same samples. …”
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MicroRNAs as novel biomarkers for prenatal fetal congenital heart disease – systematic literature review by Adrianna Kondracka, Paulina Gil-Kulik, Magda Rybak-Krzyszkowska, Jakub Staniczek, Anna Oniszczuk, Bartosz Kondracki

“…The included studies utilized advanced technologies, such as next-generation sequencing (e.g., Illumina HiSeq, NovaSeq) and microarrays, for discovery-phase experiments, while validation predominantly employed qRT-PCR techniques. …”
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Value of Optical Genome Mapping (OGM) for Diagnostics of Rare Diseases: A Family Case Report by Kovanda A, Miljanović O, Lovrečić L, Maver A, Hodžić A, Peterlin B

“…Optical genome mapping (OGM) is a novel method enabling the detection of structural genomic variants. The method is based on the laser image acquisition of single, labeled, high-molecular-weight DNA molecules and can detect structural genomic variants such as translocations, inversions, insertions, deletions, duplications, and complex structural rearrangements. …”
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Novel genotyping algorithms for rare variants significantly improve the accuracy of Applied Biosystems™ Axiom™ array genotyping calls: Retrospective evaluation of UK Biobank array... by Orna Mizrahi-Man, Marcos H Woehrmann, Teresa A Webster, Jeremy Gollub, Adrian Bivol, Sara M Keeble, Katherine H Aull, Anuradha Mittal, Alan H Roter, Brant A Wong, Jeanette P Schmidt

“…The UK Biobank genotyped about 500k participants using Applied Biosystems Axiom microarrays. Participants were subsequently sequenced by the UK Biobank Exome Sequencing Consortium. …”
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Diagnosing Autoimmune Bullous Diseases—An Indian Perspective by Adhyatm Bhandari, Dipankar De, Shikha Shah, Debajyoti Chatterjee, Vinod Kumar, Rahul Mahajan, Sanjeev Handa

“…It uses microarrays with multiple antigenic substrates to simultaneously screen common AIBDs. …”
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Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling by Nikole J. Byrne, Christoph Koentges, Elisabeth Khan, Katharina Pfeil, Robert Sandulescu, Sayan Bakshi, Carolin Költgen, Ivan Vosko, Johannes Gollmer, Thomas Rathner, Günter Roth, Michael M. Hoffmann, Katja E. Odening, Hauke Horstmann, Luke A. Potter, Christoph Bode, Dennis Wolf, Harald Sourij, Senka Ljubojevic-Holzer, Markus Wallner, Peter P. Rainer, Simon Sedej, Daniel Scherr, Dirk von Lewinski, Adam R. Wende, Andreas Zirlik, Heiko Bugger

“…To current study was designed to explore mechanisms underlying adverse effects of increased SIRT4 levels in the heart following pressure overload. …”
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