In vitro methodologies to evaluate nanocarriers for cancer treatment: where are we? by Giovanna Cassone Salata, Camila Megumi Hirokawa, Giovanna Barros de Melo, Isabella Draszesski Malagó, Jessica Ribeiro Nunes, Julia Sapienza Passos, Regina Gomes Daré, Luciana B. Lopes

“…Abstract Cancer treatment continues to face significant challenges, including lack of selectivity, systemic toxicity, and the adaptive resistance mechanisms of cancer cells, which limit the effectiveness of conventional therapies. …”
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Genetic heterogeneity in familial forms of genetic generalized epilepsy: from mono- to oligogenism by Maha Dahawi, Jean-Madeleine de Sainte Agathe, Mohamed S. Elmagzoub, Elhami A. Ahmed, Julien Buratti, Thomas Courtin, Eric Noé, Julie Bogoin, Bruno Copin, Fatima A. Elmugadam, Wasma A. Abdelgadir, Ahmed K. M. A. Ahmed, Mohamed A. Daldoum, Rayan Mamoon Ibrahim Altayeb, Mohamed Bashir, Leena Mohamed Khalid, Sahar Gamil, Sara Baldassari, Liena Elsayed, Boris Keren, Gregory Nuel, Ammar E. Ahmed, Eric Leguern

“…Moreover, a polygenic mode of inheritance is suspected in most cases. We performed SNP microarrays and whole exome sequencing in 20 families from Sudan, focusing on those with at least four affected members. …”
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Membranous Overexpression of Fibronectin Predicts Microvascular Invasion and Poor Survival Outcomes in Patients with Hepatocellular Carcinoma by Yoon Jung Hwang, Hyejung Lee, Suk Kyun Hong, Su Jong Yu, Haeryoung Kim

“…Methods: Immunohistochemical analysis of FN was conducted using tissue microarrays from 258 surgically resected HCCs and matched nontumorous liver tissues. …”
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Advancing Precision Medicine: The Role of Genetic Testing and Sequencing Technologies in Identifying Biological Markers for Rare Cancers by Joviana Farhat, Lara Alzyoud, Mohammad AlWahsh, Animesh Acharjee, Basem Al‐Omari

“…Results In practice, fluorescence in situ hybridization, karyotype, microarrays and other genetic tests are mainly applied to identify specific genetic alterations and mutations associated with cancer progression. …”
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Prevalence of βIII-tubulin (TUBB3) expression in human normal tissues and cancers by Fermín Person, Waldemar Wilczak, Claudia Hube-Magg, Christoph Burdelski, Christina Möller-Koop, Ronald Simon, Mercedes Noriega, Guido Sauter, Stefan Steurer, Susanne Burdak-Rothkamm, Frank Jacobsen

“…To systematically investigate the epidemiology of TUBB3 expression in normal and neoplastic tissues, we used tissue microarrays for analyzing the immunohistochemically detectable expression of TUBB3 in 3911 tissue samples from 100 different tumor categories and 76 different normal tissue types. …”
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Placental and immune cell DNA methylation reference panel for bulk tissue cell composition estimation in epidemiological studies by Kyle A. Campbell, Justin A. Colacino, John Dou, Dana C. Dolinoy, Sung Kyun Park, Rita Loch-Caruso, Vasantha Padmanabhan, Kelly M. Bakulski

“…We collated new and existing cell type DNAm profiles quantified via Illumina EPIC or 450k microarrays. To estimate cell composition, we deconvoluted whole placental samples (n = 36) with robust partial correlation based on the top 30 hyper- and hypomethylated sites identified per cell type. …”
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Serum extracellular vesicles 3’tRF-ThrCGTand 3’tRF-mtlleGAT combined with tumor markers can serve as minimally invasive diagnostic predictors for colorectal cancer by Jiefei Peng, Jiefei Peng, Fan Bu, Fan Bu, Lei Duan, Lei Duan, Anna Song, Guojun Wang, Zhijun Zhang, Zhijun Zhang

“…Gene microarrays were screened for differentially expressed tRF-RNA. …”
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urg1: a uracil-regulatable promoter system for fission yeast with short induction and repression times. by Stephen Watt, Juan Mata, Luis López-Maury, Samuel Marguerat, Gavin Burns, Jürg Bähler

“…The kanMX6 and natMX6 markers allow selection under urg1 induced and repressed conditions, respectively. …”
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Quantification of the heterogeneity of prognostic cellular biomarkers in ewing sarcoma using automated image and random survival forest analysis. by Claudia Bühnemann, Simon Li, Haiyue Yu, Harriet Branford White, Karl L Schäfer, Antonio Llombart-Bosch, Isidro Machado, Piero Picci, Pancras C W Hogendoorn, Nicholas A Athanasou, J Alison Noble, A Bassim Hassan

“…Using material from cohorts of genetically diagnosed Ewing sarcoma with EWSR1 chromosomal translocations, confocal images of tissue microarrays were segmented with level sets and watershed algorithms. …”
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Plasma CircCYP24A1 as a Novel Biomarker of Esophageal Squamous Cell Carcinoma by Ruolan Zhang M.M., Jianlin Liu M.M., Hang Yang M.M., Jinsong Tan M.M., Rong Xiong M.M., Yun Liu MM, Gang Feng PhD, Guiqin Song PhD, Kang Liu PhD

“…Fluorescence in situ hybridization was employed to detect the expression of circCYP24A1 in ESCC tissue microarrays, aiming to assess the expression of circCYP24A1 in a large population and its correlation with clinical indicators. …”
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Five immune‐gene‐signatures participate in the development and pathogenesis of Kawasaki disease by Han Nie, Shizhi Wang, Quanli Wu, Danni Xue, Weimin Zhou

“…Methods Kawasaki disease‐related datasets GSE18606, GSE68004, and GSE73461 were downloaded from the Gene Expression Omnibus database. Three microarrays were integrated and standardized to include 173 Kawasaki disease samples and 101 normal samples. …”
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Interaction networks among miRNA, protein, and metabolite fingerprints identify the regulatory networks and key players in the pathogenesis of diabetic cardiomyopathy by Bhaswati Chatterjee, Suman S. Thakur

“…The miRNA–protein interaction included evidence from TarBase and microarrays/HITS-CLIP. The protein–protein, metabolite–metabolite, and protein–metabolite interaction networks were obtained at high confidence scores (≥0.7 or 70%). …”
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Rotterdam Oncology Documentation (RONCDOC) – a high-quality data warehouse and tissue collection for head and neck cancer by Arta Hoesseini, Emilie A. C. Dronkers, Eveline Dieleman, Maria J. De Herdt, Marjan. H. Wieringa, Marie-Louise F. van Velthuysen, Marinella P. J. Offerman, Aniel Sewnaik, Dominiek Monserez, Stijn Keereweer, Jose A. U. Hardillo, Robert Jan Baatenburg de Jong

“…Furthermore, a comprehensive validation protocol was developed to guarantee the quality of the data. Tissue microarrays (TMAs) were constructed from resection specimens of patients with primary oral squamous cell carcinoma. …”
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Low density of intratumoral M1-macrophage infiltration may correlate with worse prognosis in low-grade early-stage uterine endometrioid carcinoma by Michiko Nagamine, Hiroshi Ogi, Maki Hirai, Ikoi Omatsu, Sanzo Moriwaki, Saya Shibata, Satoru Yasukawa, Kaori Yoriki, Motohiro Kojima, Taisuke Mori, Kyoko Itoh, Eiichi Konishi

“…Multiplex immunohistochemistry was performed on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue blocks of primary surgical specimens and, when available, recurrent lesions. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…TLS formation in ADU S-100-treated mice was associated with the development of a highly oligoclonal TIL repertoire enriched in expanded T cell clonotypes unique to the TME and not detected in the periphery.Conclusions Our data support the premise that i.t. delivery of low-dose STING agonist promotes VN and a proinflammatory TME supportive of TLS formation, enrichment in the TIL repertoire and tumor growth control.…”
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Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data by Ting-qing Gan, Zu-cheng Xie, Rui-xue Tang, Tong-tong Zhang, Dong-yao Li, Zu-yun Li, Gang Chen

“…First, a meta-analysis was performed with eligible literature, followed by microRNA microarrays in the Gene Expression Omnibus database, to verify the diagnostic and prognostic values of miR-145-5p. …”
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ASF1B promotes gastric cancer progression by modulating H2AC20 and activating PI3K/AKT and ERK1/2 pathways by Mengyuan Zhao, Junchang Zhang, Yanjun He, Chongge You

“…With the continuous enrichment of treatment methods, GC has entered an era of comprehensive treatment oriented toward precision and standardization.MethodsThrough the application of bioinformatics and assessments of tissue microarrays, this study has selected the histone chaperone Anti-Silencing Function 1B (ASF1B) for detailed analysis, including clinical specimens. …”
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Urine miRNA signature as potential non-invasive diagnostic biomarker for Hirschsprung’s disease by Abhijit Sreepada, Abhijit Sreepada, Rasul Khasanov, Enas Zoheer Elkrewi, Enas Zoheer Elkrewi, Carolina de la Torre, Judith Felcht, Ahmad A. Al Abdulqader, Ahmad A. Al Abdulqader, Richard Martel, Nicolás Andrés Hoyos-Celis, Michael Boettcher, Lucas M. Wessel, Karl-Herbert Schäfer, María Ángeles Tapia-Laliena

“…Results from 5 HSCR patients using microarrays, revealed hsa-miR-378 h, hsa-miR-210-5p, hsa-miR-6876-3p, hsa-miR-634 and hsa-miR-6883-3p as the most upregulated miRNAs; while hsa-miR-4443, hsa-miR-22-3p, hsa-miR-4732-5p, hsa-miR-3187-5p, and hsa-miR-371b-5p where the most downregulated miRNAs. …”
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Mesangial cell-derived CircRNAs in chronic glomerulonephritis: RNA sequencing and bioinformatics analysis by Ji Hui Fan, Xiao Min Li

“…Pro-inflammatory cytokines and cell cycle stages were detected by Enzyme-linked immunosorbent assay (ELISA) and Flow Cytometry experiment, respectively. …”
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Characterization of tumor-infiltrating lymphocytes and their spatial distribution in triple-negative breast cancer by Eunkyung Han, Hye Yeon Choi, Hyun Jung Kwon, Yul Ri Chung, Hee-Chul Shin, Eun-Kyu Kim, Koung Jin Suh, Se Hyun Kim, Jee Hyun Kim, So Yeon Park

“…Methods We analyzed TNBC samples through PanCancer Immune Profiling using NanoString nCounter assays to identify immune-related genes that are expressed differentially in relation to TIL levels and evaluated protein expression of selected markers through immunohistochemical staining on tissue microarrays. …”
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