Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle by Lisa Abdank, Igor Loncaric, Sascha D. Braun, Elke Müller, Stefan Monecke, Ralf Ehricht, Reinhild Krametter-Frötscher

“…Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. No methicillin-resistant <i>Staphylococcus aureus</i> was found in the milk samples, but one nasal swab was positive for methicillin-resistant <i>Staphylococcus aureus</i>. …”
Get full text

Inhibition of ATG5-mediated autophagy maintains PMAIP1 stability to promote cell apoptosis and suppress triple-negative breast cancer progression by Fangjian Shang, Hongfeng Nie, Liyan Du, Jin Shang, Xiangquan Song, Ya Chen, Hui Li, Zhuo Wang, Yixin Qi, Liyan Zhao

“…Methods TNBC-related datasets were retrieved from The Cancer Genome Atlas and selected by Prediction Analysis of Microarray 50 analysis to assess the expression of PMAIP1 in the samples. …”
Get full text

Performance and clinical implications of non-invasive prenatal testing for rare chromosomal abnormalities: a retrospective study of 94,125 cases by Haimei Qi, Haijun Chen, Zhuling Zhang, Jinhui Gan, Huifeng Liu, Xianping Yuan, Fuyu Luo, Junkun Chen, Shichun Shen

“…BackgroundNon-invasive prenatal testing (NIPT) has demonstrated robust performance in detecting common trisomies and copy number variations. …”
Get full text

Determination of Viral Nucleic Acid in the Human Blood by M. A. Abdurashitov, N. A. Netesova

“…Methods such as in situ hybridization, reverse transcription reaction (RT-PCR), nested PCR, multiplex PCR, as well as DNA microarray technology, and the method of massive parallel sequencing are considered in detail. …”
Get full text

Integrative analysis of T cell-mediated tumor killing-related genes reveals KIF11 as a novel therapeutic target in esophageal squamous cell carcinoma by Xinxin Cheng, Huihui Zhao, Zhangwang Li, Liping Yan, Qingjie Min, Qingnan Wu, Qimin Zhan

“…The KIF11 expression was detected by multiplex fluorescent immunohistochemistry on tissue microarray from ESCC patients. …”
Get full text

Sequential prenatal diagnosis of fetal skeletal dysplasia: A cohort study by Mengting Jiang, Bin Zhang, Jing Wang, Wei Qiao, Xiuzhen Mao, Bin Yu

“…Abstract Introduction Genetic factors are considered to be the main factors leading to fetal skeletal dysplasia (SD), and chromosomal microarray analysis (CMA) has been used clinically for the detection of SD fetuses. …”
Get full text

Nanopore-based random genomic sampling for intraoperative molecular diagnosis by Francesco E. Emiliani, Abdol Aziz Ould Ismail, Edward G. Hughes, Gregory J. Tsongalis, George J. Zanazzi, Chun-Chieh Lin

“…Results In our retrospective cohort of 26 malignant brain tumors, iSCORED demonstrated 100% concordance in CNV detection, including chromosomal alterations and oncogene amplifications, when compared to clinically validated assays such as Next-Generation Sequencing and Chromosomal Microarray. …”
Get full text

Long read whole genome sequencing-based discovery of structural variants and their role in aetiology of non-syndromic autism spectrum disorder in India by Jhanvi Shah, Debasrija Mondal, Deepika Jain, Priti Mhatre, Ketan Patel, Anand Iyer, Manoj Pandya, Bhargavi Menghani, Gayatri Dave, Jayesh Sheth, Frenny Sheth, Shweta Ramdas, Harsh Sheth

“…Short read sequencing (SRS) and chromosomal microarray (CMA) are unable to resolve these SVs due to their inherent technological limitations. …”
Get full text

Over-expression of either MECP2_e1 or MECP2_e2 in neuronally differentiated cells results in different patterns of gene expression. by Marija Orlic-Milacic, Liana Kaufman, Anna Mikhailov, Aaron Y L Cheung, Huda Mahmood, James Ellis, Peter J Gianakopoulos, Berge A Minassian, John B Vincent

“…DOCK8 was shown via microarray and qRT-PCR to be upregulated in both SK-N-SH cells and mouse fibroblasts. …”
Get full text

Gene network inference and biochemical assessment delineates GPCR pathways and CREB targets in small intestinal neuroendocrine neoplasia. by Ignat Drozdov, Bernhard Svejda, Bjorn I Gustafsson, Shrikant Mane, Roswitha Pfragner, Mark Kidd, Irvin M Modlin

“…High throughput techniques such as inference of gene regulatory networks from microarray experiments can objectively define signaling machinery in this disease. …”
Get full text

Metabolomic characterisation of the glioblastoma invasive margin reveals a region-specific signature by James Wood, Stuart J. Smith, Marcos Castellanos-Uribe, Anbarasu Lourdusamy, Sean T. May, David A. Barrett, Richard G. Grundy, Dong-Hyun Kim, Ruman Rahman

“…Here, we use liquid chromatography–mass spectrometry and gene expression microarray to profile integrated intratumour metabolic heterogeneity, as a direct functional readout of adaptive responses of subclones to the tumour microenvironment. …”
Get full text

Combined Use of Gene Expression Modeling and siRNA Screening Identifies Genes and Pathways Which Enhance the Activity of Cisplatin When Added at No Effect Levels to Non-Small Cell... by Ada W Y Leung, Stacy S Hung, Ian Backstrom, Daniel Ricaurte, Brian Kwok, Steven Poon, Steven McKinney, Romulo Segovia, Jenna Rawji, Mohammed A Qadir, Samuel Aparicio, Peter C Stirling, Christian Steidl, Marcel B Bally

“…Here, we report on studies designed to uncover how tumour cells respond to sub-lethal doses of cisplatin. A microarray study revealed changes in gene expressions that occurred when A549 cells were exposed to a no-observed-effect level (NOEL) of cisplatin (e.g. the IC10). …”
Get full text

A stratified transcriptomics analysis of polygenic fat and lean mouse adipose tissues identifies novel candidate obesity genes. by Nicholas M Morton, Yvonne B Nelson, Zoi Michailidou, Emma M Di Rollo, Lynne Ramage, Patrick W F Hadoke, Jonathan R Seckl, Lutz Bunger, Simon Horvat, Christopher J Kenyon, Donald R Dunbar

“…Selected candidate genes Npr3 and Thbs1, as well as Gys2, a non-QTL gene that otherwise passed our enrichment criteria were characterised, revealing novel functional effects consistent with a contribution to obesity.…”
Get full text

Comparative linkage meta-analysis reveals regionally-distinct, disparate genetic architectures: application to bipolar disorder and schizophrenia. by Brady Tang, Tricia Thornton-Wells, Kathleen D Askland

“…Since it is likely a majority of common variants of modest effect have been identified through the application of tagSNP-based microarray platforms (i.e., GWAS), alternative approaches robust to detection of low-frequency (1-5% MAF) and rare (<1%) variants are of great importance. …”
Get full text

Concave Magnetic-Responsive Hydrogel Discs for Enhanced Bioassays by Amin Ghaffarzadeh Bakhshayesh, Huiyan Li

“…This approach enables immediate preparation and customization of hydrogel properties such as porosity, magnetic responsiveness, and embedded particles and is adaptable for use with microarray printers. The concave design increased the surface area by 43% compared to conventional hemispherical hydrogels, enhancing diffusion rates and accelerating reactions. …”
Get full text

Hypoxia-Induced miR-210 Promotes Endothelial Cell Permeability and Angiogenesis via Exosomes in Pancreatic Ductal Adenocarcinoma by Guo Wu, Xiaojie Ding, Gang Quan, Jianwei Xiong, Qiang Li, Zhonghu Li, Yaqin Wang

“…The aim of this article was to identify and investigate abnormally expressed miRNAs in PDAC exosomes in vivo and in vitro. Methods. Microarray studies were used to detect aberrantly expressed miRNAs in PDAC exosomes, and miR-210 expression in cells or exosomes was further analyzed by qRT-PCR. …”
Get full text

RNA-Seq profiling reveals novel hepatic gene expression pattern in aflatoxin B1 treated rats. by B Alex Merrick, Dhiral P Phadke, Scott S Auerbach, Deepak Mav, Suzy M Stiegelmeyer, Ruchir R Shah, Raymond R Tice

“…PCA and cluster analysis of DEGs showed clear separation between AFB1 and CTRL treatments and concordance among group replicates. qPCR of eight high and medium DEGs and three low DEGs showed good comparability among RNA-Seq and microarray transcripts. DESeq analysis identified 1,026 differentially expressed transcripts at greater than two-fold change (p<0.005) compared to 626 transcripts by microarray due to base pair resolution of transcripts by RNA-Seq, probe placement within transcripts or an absence of probes to detect novel transcripts, splice variants and exons. …”
Get full text

Genetic test results and one-year developmental outcomes of fetuses with congenital heart disease by Hui Hu, Bo Zhou, Qunda Shan, Shuangshuang Shen, Xiangdong Zhang, Penglong Chen, Jiao Liu, Xiaofang Lan

“…The detection rate of CNV was significantly lower in fetuses with isolated cardiac abnormalities (P &lt; 0.05). …”
Get full text

Characterization of a MERS-related betacoronavirus in Danish brown long-eared bats (Plecotus auritus) by Camille Melissa Johnston, Vithiagaran Gunalan, Hans J. Baagøe, Anna S. Fomsgaard, Charlotta Polacek, Morten Rasmussen, Louise Lohse, Thomas Bruun Rasmussen

“…Positive samples underwent ORF1b sequencing, microarray analysis and Illumina MiSeq sequencing, followed by metagenomic assembly of full-length genomes. …”
Get full text

Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy by Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du

“…Conclusions Our study demonstrates that CD155 is selectively expressed in cancer cells while being rarely detected in normal tissues, and may serve as a promising pan-cancer target for CAR-T therapy. …”
Get full text