Examination of the functions and mechanism of KCP in mediating paclitaxel resistance in cervical squamous carcinoma cells by Yue He, Jian-Qing Xu, Jing-Jing Zhang, Zhen-You Liu, Chen Ji, Yang Liu, Yun-Fan Wang, Ming Wang, Yu-Mei Wu, Yan Wang

“…Key cell functions were assessed by colony formation assay, measurement of cell proliferation by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and FACS-based detection of apoptosis. The downstream mechanism of KCP-mediated resistance to paclitaxel was then examined using human gene chip detection and IPA bioinformatics analysis, and qPCR analysis was used to validate its downstream genes.Results①Functional studies of SiHa cells showed that KCP knockout (sgRNA) inhibited colony formation and proliferation of SiHa cells in the presence of paclitaxel (p<0.05). ②Using a whole human genome microarray, a total of 491 differentially expressed genes were identified in KCP knockout versus the NC SiHa cells. …”
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The impact of the nucleosome code on protein-coding sequence evolution in yeast. by Tobias Warnecke, Nizar N Batada, Laurence D Hurst

“…We identify nucleosome positioning as a likely candidate to set up such a DNA-level selective regime and use high-resolution microarray data in yeast to compare the evolution of coding sequence bound to or free from nucleosomes. …”
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A multi-omic meta-analysis reveals novel mechanisms of insecticide resistance in malaria vectors by Sanjay C. Nagi, Victoria A. Ingham

“…This study, employing a cross-species approach, integrates RNA-Sequencing, whole-genome sequencing, and microarray data to elucidate drivers of insecticide resistance in Anopheles gambiae complex and An. funestus. …”
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Profiling of gastric cancer cell-surface markers to achieve tumour–normal discrimination by Henry Yang, Khek Yu Ho, Judith Toh, Michal Marek Hoppe, Teena Thakur, Kar Tong Tan, Brendan Pang, Sharmaine Ho, Rony Roy, Khay Guan Yeoh, Raghav Sundar, Anand Jeyasekharan

“…Promising candidates from this analysis were validated orthogonally using multispectral immunohistochemistry (mIHC) with automated quantitative analysis using the Vectra platform. mIHC was performed on a tissue microarray containing matched normal, marginal and tumour tissues. …”
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Aberrant expression of shared master-key genes contributes to the immunopathogenesis in patients with juvenile spondyloarthritis. by Lovro Lamot, Fran Borovecki, Lana Tambic Bukovac, Mandica Vidovic, Marija Perica, Kristina Gotovac, Miroslav Harjacek

“…Microarray results and bioinformatical analysis revealed 745 differentially expressed genes involved in various inflammatory processes, while qRT-PCR analysis of selected genes confirmed data universality and specificity of expression profiles in jSpA patients. …”
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Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy by Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du

“…Conclusions Our study demonstrates that CD155 is selectively expressed in cancer cells while being rarely detected in normal tissues, and may serve as a promising pan-cancer target for CAR-T therapy. …”
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NF1 expression profiling in IDH-wildtype glioblastoma: genomic associations and survival outcomes by Michael Chang, Mohamed Sherief, Maria Ioannou, Viveka Chinnasamy, Lucy Chen, Michael Frost, Michelle Mattson-Hoss, Herb Sarnoff, David O. Kamson, Matthias Holdhoff, Debraj Mukherjee, Chetan Bettegowda, Jordina Rincon-Torroella, Victoria Croog, Peng Huang, Fausto J. Rodriguez, Calixto-Hope G. Lucas, Karisa C. Schreck

“…Methods We assembled a single-institution, retrospective cohort of 542 IDH-wildtype GBM with somatic next generation sequencing to investigate the frequency and nature of detected NF1 alterations. We selected 69 GBMs from which to build a tissue microarray (TMA) of 44 NF1-wildtype and 25 NF1-mutant cases. …”
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A retrospective analysis of prenatal genetic results in fetal hydronephrosis. by Keqin Jin, Xiayuan Xu, Yue Qian, Liping Zhang, Min Hu, Jianfeng Luo

“…<h4>Objective</h4>To discuss the application value of technologies such as chromosome microarray analysis (CMA) in fetuses with hydronephrosis and pyelectasis.…”
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Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease by Xiaoyan Qu, Xiaomin Dang, Weijia Wang, Ying Li, Dan Xu, Dong Shang, Ying Chang

“…Methods. lncRNA and mRNA profiling of PBMCs from 17 smokers and 14 COPD subjects was detected by high-throughput microarray. The expression of dysregulated lncRNAs was validated by qPCR. …”
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Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation by Jesper Karlsson, Lina Wirestam, Hanna Duàn, Suhana Ahmad, Daniel Appelgren, Helena Enocsson, Jonas Wetterö, Christopher Sjöwall

“…The levels of anti-CRP and SLE-associated antibodies were assessed, and a microarray-based linear epitope mapping was performed to detect binding sites on the full CRP monomer. …”
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Gene Expression Profiling in Organ Transplantation by Osama Ashry Ahmed Gheith

“…However, because microarray analyses are expensive and time consuming and their statistical evaluation is often very difficult, gene expression analysis using the RTPCR method is nowadays recommended. …”
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Exploring Flexible Penalization of Bayesian Survival Analysis Using Beta Process Prior for Baseline Hazard by Kazeem A. Dauda, Ebenezer J. Adeniyi, Rasheed K. Lamidi, Olalekan T. Wahab

“…Moreover, in microarray research, it is common to identify groupings of genes involved in the same biological pathways. …”
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A retrospective analysis of 6942 amniocentesis cases by Qingsha An, Yuxiao Huang, Feifei Yu, Yilun Tao, Juan Li, Xiaoze Li

“…Among the single-method cases, Chromosomal Microarray Analysis (CMA) was the most frequently selected technique (14.18%). …”
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Synchrotron Radiation-Induced X-Ray Emission to Identify Metal Ions in Preparations of Purified Protein by Thomas Pfister, Huan Feng, Eckard Wimmer, Keith W. Jones

“…The suitability of synchrotron radiation-induced X-ray emission (SRIXE) for the detection and identification of metal ions in preparations of purified, soluble proteins was tested. …”
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Prevalence and Diversity of <i>Staphylococcus aureus</i> in Bulk Tank Milk from Community-Based Alpine Dairy Pastures in Tyrol, Austria by Nasrin Ramezanigardaloud, Igor Loncaric, Patrick Mikuni-Mester, Masoumeh Alinaghi, Monika Ehling-Schulz, Johannes Lorenz Khol, Tom Grunert

“…A total of 140 isolates collected from the 94 <i>S. aureus</i>-positive community-based Alpine dairy pastures revealed 33 distinct <i>spa</i> types, with t2953 (<i>n</i> = 33), t529 (<i>n</i> = 12), t267 (<i>n</i> = 11), and t024 (<i>n</i> = 10) being the most common. Selected isolates representing the different <i>spa</i> types were characterised by DNA microarray-based genotyping, multi-locus sequence typing (MLST), and antimicrobial susceptibility testing. …”
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Exploration of autophagy-related molecular mechanisms underlying epilepsy using multiple datasets by Yongfei Wang, Haoxuan Zeng, Chongxu Liu, Jianjun Chen, Yihong Huang, Xianju Zhou

“…Methods We analyzed GSE143272 and GSE4290 microarray datasets from the NCBI Gene Expression Omnibus database, which is established based on evaluations of peripheral blood samples. …”
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Identification of sero-reactive antigens for the early diagnosis of Johne's disease in cattle. by Lingling Li, John P Bannantine, Joseph J Campo, Arlo Randall, Yrjo T Grohn, Robab Katani, Megan Schilling, Jessica Radzio-Basu, Vivek Kapur

“…JD has a high herd prevalence and causes serious animal health problems and significant economic loss in domesticated ruminants throughout the world. Since serological detection of MAP infected animals during the early stages of infection remains challenging due to the low sensitivity of extant assays, we screened 180 well-characterized serum samples using a whole proteome microarray from Mycobacterium tuberculosis (MTB), a close relative of MAP. …”
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Identification of Differentially Expressed Genes and Elucidation of Pathophysiological Relevance of ABCA1 in HaCaT Cells Induced by PM2.5 by Fen Peng, Chen-Hong Xue, Xiao-Jing Yang, Jing-Yi Huang, Zhou Chen, Jian-Zhong Zhang

“…Microarray analyses were used to find out the microarray gene expression profiling; data processing included gene enrichment and pathway analysis. …”
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Analysis of gene expression difference and biological process in chorionic villi of unexplained recurrent spontaneous abortion by Linling Shi, Lun Wei, Meiqiu Lu, Hongmei Ding, Le Bo

“…Abstract Objective To explore the biological relationship between the regulatory signal pathways involved in differentially expressed genes and recurrent spontaneous abortion (RSA) by analyzing the gene expression microarray data of unexplained RSA. Methods The gene expression profile data of chorionic villi from unexplained recurrent abortion with normal karyotype and selective induced abortion were compared. …”
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Adenosine Receptor 3 in Liver Cancer: Expression Variability, Epigenetic Modulation, and Enhanced Histone Deacetylase Inhibitor Effects by Louise Kaldjob-Heinrich, Sandro Nuciforo, Steffen Lemke, Aaron Stahl, Stefan Czemmel, Sepideh Babaei, Lauriane Blukacz, Marie-Anne Meier, Yizheng Zhang, Christian M. Schürch, Irene Gonzalez-Menendez, Pascal Woelffing, Nisar P. Malek, Veit Scheble, Sven Nahnsen, Manfred Claassen, Markus Templin, Hans Bösmüller, Markus H. Heim, Daniel Dauch, Michael Bitzer

“…However, further data are required to clarify ADORA3 expression patterns in liver cancer, mechanisms of action, and the potential for combination therapies to inform patient selection for future clinical trials. Methods: Patient-derived tissue microarrays and RNA-sequencing were employed to investigate ADORA3 expression. …”
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