Molecular profiling analysis for colorectal cancer patients with Pi-Xu or Shi-Re syndrome by Xinbing Sui, Yong Guo, Wei Ni, Haiyan Jin, Haoming Lin, Tian Xie

“…The protein expression was determined by Immunohistochemistry (IHC) staining and mRNA profiling was detected by expression microarray. Furthermore, mRNA fold change was evaluated by qRT-PCR. …”
Get full text

Etiology and outcomes of fetal renal abnormalities in Southern China: a single-tertiary-center study by Meiying Cai, Yashi Gao, Huili Xue, Xianguo Fu, Hua Cao, Liangpu Xu, Na Lin, Hailong Huang

“…We retrospectively analyzed data from 1,019 cases for which chromosomal microarray analysis (CMA) was performed; 58 CMA-negative fetuses were selected for whole-exome sequencing (WES). …”
Get full text

Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5’tRFAla/DVL/β-catenin pathway inhibition by Zhipeng Zhang, Yanmei Wu, Wenqiang Liang, Zhifang Liao, Hongbo Liao, Xingxing Xing, Wenxin Yi, Zixuan Liu, Yicheng Li, Mengya Shi, Dongling Lin, Ting Gu, Biao Wu, Mingzhi Zou, Huilai Miao, Xin Wu

“…Subsequently, two new tsRNAs, namely 5’tRFAla and 5’tiRNAAla, which match well with the mRNAs of two pivotal upstream regulators (DVL2 and DVL3) of β-catenin based on bioinformatics analyses, were detected to be significantly decreased in ELT-treated PLC/PRF/5 cells using Arraystar small RNA microarray analyses. …”
Get full text

MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
Get full text

A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response by Xiao‐Ming Lyu, Xiao‐Wei Zhu, Manli Zhao, Xian‐Bo Zuo, Zhong‐Xi Huang, Xiao Liu, Tao Jiang, Xue‐Xi Yang, Xin Li, Xiao‐Bing Long, Jian‐Guo Wang, Jin‐Bang Li, Ming‐Yu Han, Shuang Wang, Teng‐Fei Liu, Bo Zhang, Tao Sun, Zhi Cheng, Mo‐Chang Qiu, Lei Dong, Lu Zheng, Long‐Cheng Zhang, Jia‐Hong Wang, Gan‐Guan Wei, Kaitai Yao, Qian Wang, Hou‐Feng Zheng, Xin Li

“…Significance analysis of microarray suggested that TRIM26 downregulation was related to low immune response in NPC. …”
Get full text

Analysis of Result Variability from High-Density Oligonucleotide Arrays Comparing Same-Species and Cross-Species Hybridizations by J.D. Chismar, T. Mondala, H.S. Fox, E. Roberts, D. Langford, E. Masliah, D.R. Salomon, S.R. Head

“…There exists a significant limitation in the variety of organisms for which microarrays have been developed because of a lack of genomic sequence data. …”
Get full text

FGFR3-TACC3 fusion gene promotes glioblastoma malignant progression through the activation of STAT3 signaling pathway by Yiming Li, Yiming Li, Jianshen Liang, Jianshen Liang, Jianshen Liang, Xiude Ren, Xiude Ren, Jiahe Guo, Jiahe Guo, Jiahe Guo, Xisen Wang, Xisen Wang, Xisen Wang, Xuya Wang, Xuya Wang, Xuya Wang, Shengping Yu, Shengping Yu, Tao Li, Tao Li, Xuejun Yang, Xuejun Yang, Xuejun Yang

“…A series of phenotypic experiments, including CCK-8, EdU, colony-formation assay, wound healing assay, and transwell assay were conducted to detect the effects of F3-T3 on proliferation, invasion, and migration of glioma cells. …”
Get full text

MicroRNAs as novel biomarkers for prenatal fetal congenital heart disease – systematic literature review by Adrianna Kondracka, Paulina Gil-Kulik, Magda Rybak-Krzyszkowska, Jakub Staniczek, Anna Oniszczuk, Bartosz Kondracki

“…The included studies utilized advanced technologies, such as next-generation sequencing (e.g., Illumina HiSeq, NovaSeq) and microarrays, for discovery-phase experiments, while validation predominantly employed qRT-PCR techniques. …”
Get full text

Value of Optical Genome Mapping (OGM) for Diagnostics of Rare Diseases: A Family Case Report by Kovanda A, Miljanović O, Lovrečić L, Maver A, Hodžić A, Peterlin B

“…Optical genome mapping (OGM) is a novel method enabling the detection of structural genomic variants. The method is based on the laser image acquisition of single, labeled, high-molecular-weight DNA molecules and can detect structural genomic variants such as translocations, inversions, insertions, deletions, duplications, and complex structural rearrangements. …”
Get full text

Novel genotyping algorithms for rare variants significantly improve the accuracy of Applied Biosystems™ Axiom™ array genotyping calls: Retrospective evaluation of UK Biobank array... by Orna Mizrahi-Man, Marcos H Woehrmann, Teresa A Webster, Jeremy Gollub, Adrian Bivol, Sara M Keeble, Katherine H Aull, Anuradha Mittal, Alan H Roter, Brant A Wong, Jeanette P Schmidt

“…The UK Biobank genotyped about 500k participants using Applied Biosystems Axiom microarrays. Participants were subsequently sequenced by the UK Biobank Exome Sequencing Consortium. …”
Get full text

Novel Hypomorphic Mutation in FANCD2 Gene Observed in a Fetus with Multiple Congenital Anomalies by Radoslava Vazharova, Svetlana Vragaleva, Violeta Dimitrova, Samuil Ivanov, Lubomir Balabanski, Maxim Malinov, Draga Toncheva

“…The case illustrates that patients/families affected by rare monogenic disorders may benefit from application of modern technologies like microarrays and NGS.…”
Get full text

Effect of PM2.5 exposure on susceptibility to allergic asthma in elderly rats treated with allergens by Lianlian Zhao, Xiaolin Ding, Li Zhou, Chenchen Song, Taisheng Kang, Yanfeng Xu, Yunpeng Liu, Yunlin Han, Wenjie Zhao, Boxiang Zhang, Dan Xu, Jianguo Guo

“…Ten metabolites and 11 metabolic pathways were uniquely detected in OVA-treated rats before PM2.5 exposure. …”
Get full text

Microsatellite Instability and Loss of Heterozygosity in CCND1 Microsatellite loci at Chr.11q13 in OSCC Patients with Therapeutic Validation by Seema Gupta, Shally Batham, Biswajit Maity

“…Molecular laboratory techniques like microarrays, microsatellites, and single-nucleotide polymorphisms (SNPs) have created new opportunities for diagnosing diseases, identifying chromosomal aberrations, and detecting point mutations for medical verification. …”
Get full text

MMP14 and DDR2 are potential molecular markers for metastatic triple-negative breast cancer by Rabeah Al-Temaimi, Rasheed Ahmad, Fahd Al-Mulla

“…Here, we utilized TNBC tissue microarrays to determine the protein expression of seven matrix metalloproteinases (MMPs) and Discoidin domain receptor 2 (DDR2) in metastatic and non-metastatic TNBC to identify potential targets for immunotherapy. …”
Get full text

Evaluation of the diagnostic concordance of FDA-approved PD-L1 assays in clear cell renal cell carcinoma by Yoko Koh, Taigo Kato, Gaku Yamamichi, Eisuke Tomiyama, Makoto Matsushita, Koji Hatano, Atsunari Kawashima, Norio Nonomura, Motohide Uemura

“…In this study, we constructed tissue microarrays from 286 clear cell renal cell carcinoma (ccRCC) tissue samples and evaluated PD-L1 expression using the 22C3, 28 − 8, SP142, and SP263 PD-L1 assays. …”
Get full text

HERV activation segregates ME/CFS from fibromyalgia while defining a novel nosologic entity by Karen Giménez-Orenga, Eva Martín-Martínez, Lubov Nathanson, Elisa Oltra

“…By using HERV-V3 high-density microarrays (including over 350k HERV elements and more than 1500 immune-related genes) to interrogate the transcriptomes of peripheral blood mononuclear cells from female patients diagnosed with ME/CFS, FM, or both, and matched healthy controls (n = 43), this study fills this gap of knowledge. …”
Get full text

A Narrative Review of Molecular, Immunohistochemical and In-Situ Techniques in Dermatopathology by J. A. Gabriel, N. Weerasinghe, P. Balachandran, R. Salih, G. E. Orchard

“…Gene expression profiling techniques, such as microarrays and RNA sequencing, have provided insights into dysregulated pathways and molecular signatures associated with conditions ranging from inflammatory skin disorders to cutaneous malignancies. …”
Get full text

Survivin Expression in Placentas with Intrauterine Growth Restriction by Pavo Perković, Sanja Štifter-Vretenar, Marina Perković, Marko Štefančić, Ena Holjević, Andrea Dekanić, Tea Štimac

“…<b>Methods</b>: Survivin presence was determined in 153 archival formalin-fixed and paraffin-embedded placental tissues from IUGR (N = 122) and uncomplicated (N = 31) term pregnancies. Tissue microarrays (TMAs) were constructed, and survivin expression was assessed using immunohistochemistry (IHC). …”
Get full text

Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study by Bernard A Fox, Chichung Wang, Kristin Roman, Carla Coltharp, Carmen Ballesteros-Merino, Shawn M Jensen, Janis M Taube, David L Rimm, Cliff Hoyt, Mei Jiang, Edwin R Parra, Ignacio Wistuba, Elizabeth L Engle, John McGuire, Bethany Remeniuk, Darren Locke

“…Serial sections from tonsil and breast carcinoma and non-small cell lung cancer (NSCLC) tissue microarrays (TMAs), TSA-Opal fluorescent detection reagents, and antibodies were distributed to the six sites equipped with a Leica Bond Rx autostainer and a Vectra Polaris multispectral imaging platform. …”
Get full text

Effects of tumour-associated macrophages on cardia carcinoma progression by Zhixiong Zhang, Zihong Xu, Yaowu Cai, Xintong Chen, Chaoqing Huang, Xuefeng Xu

“…The THP-1 monocyte cell line was used to induce differentiation into M2-type TAMs, co-cultured with gastric cancer cell lines AGS and MKN-45, and the effect of TAMs on the invasive ability of gastric cancer cells was detected by Transwell assay. Western blotting was used to detect the expression of matrix metalloproteinase-2 (MMP-2) and its inhibitor tissue inhibitor of metalloproteinase-3 (TIMP-3) in the microarrayed tissues. …”
Get full text