Synchrotron Radiation-Induced X-Ray Emission to Identify Metal Ions in Preparations of Purified Protein by Thomas Pfister, Huan Feng, Eckard Wimmer, Keith W. Jones

“…The suitability of synchrotron radiation-induced X-ray emission (SRIXE) for the detection and identification of metal ions in preparations of purified, soluble proteins was tested. …”
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Machine learning reveals CAT gene as a novel potential diagnostic and prognostic biomarker in non-small cell lung cancer by Yi Tian, Wen-ya Zhao, Yi-ru Liu, Wen-wen Song, Qiao-xin Lin, Yan-na Gong, Yi-ting Deng, Dian-na Gu, Ling Tian

“…Furthermore, immunohistochemical staining was performed in an independent cohort from a tissue microarray. Additionally, cell culture and RT-qPCR were employed for external validation. …”
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Identification of sero-reactive antigens for the early diagnosis of Johne's disease in cattle. by Lingling Li, John P Bannantine, Joseph J Campo, Arlo Randall, Yrjo T Grohn, Robab Katani, Megan Schilling, Jessica Radzio-Basu, Vivek Kapur

“…JD has a high herd prevalence and causes serious animal health problems and significant economic loss in domesticated ruminants throughout the world. Since serological detection of MAP infected animals during the early stages of infection remains challenging due to the low sensitivity of extant assays, we screened 180 well-characterized serum samples using a whole proteome microarray from Mycobacterium tuberculosis (MTB), a close relative of MAP. …”
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Identification of Differentially Expressed Genes and Elucidation of Pathophysiological Relevance of ABCA1 in HaCaT Cells Induced by PM2.5 by Fen Peng, Chen-Hong Xue, Xiao-Jing Yang, Jing-Yi Huang, Zhou Chen, Jian-Zhong Zhang

“…Microarray analyses were used to find out the microarray gene expression profiling; data processing included gene enrichment and pathway analysis. …”
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Highly immunoreactive IgG antibodies directed against a set of twenty human proteins in the sera of patients with amyotrophic lateral sclerosis identified by protein array. by Caroline May, Eckhard Nordhoff, Swaantje Casjens, Michael Turewicz, Martin Eisenacher, Ralf Gold, Thomas Brüning, Beate Pesch, Christian Stephan, Dirk Woitalla, Botond Penke, Tamás Janáky, Dezső Virók, László Siklós, Jozsef I Engelhardt, Helmut E Meyer

“…These results led us to perform a case-control study using human protein microarrays to identify the antibody profiles of serum samples from 20 ALS patients and 20 healthy controls. …”
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Characterizing Methicillin-Resistant <i>Staphylococcus</i> spp. and Extended-Spectrum Cephalosporin-Resistant <i>Escherichia coli</i> in Cattle by Lisa Abdank, Igor Loncaric, Sascha D. Braun, Elke Müller, Stefan Monecke, Ralf Ehricht, Reinhild Krametter-Frötscher

“…Isolates were characterized by a polyphasic approach including susceptibility pheno- and genotyping and microarray-based assays. No methicillin-resistant <i>Staphylococcus aureus</i> was found in the milk samples, but one nasal swab was positive for methicillin-resistant <i>Staphylococcus aureus</i>. …”
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Inhibition of ATG5-mediated autophagy maintains PMAIP1 stability to promote cell apoptosis and suppress triple-negative breast cancer progression by Fangjian Shang, Hongfeng Nie, Liyan Du, Jin Shang, Xiangquan Song, Ya Chen, Hui Li, Zhuo Wang, Yixin Qi, Liyan Zhao

“…Methods TNBC-related datasets were retrieved from The Cancer Genome Atlas and selected by Prediction Analysis of Microarray 50 analysis to assess the expression of PMAIP1 in the samples. …”
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Cytokine and Antibody Based Diagnostic Algorithms for Sputum Culture-Positive Pulmonary Tuberculosis. by Tao Chen, Jinfei Lin, Wei Wang, Joy Fleming, Liang Chen, Yunxia Wang, Haicheng Li, Huixin Guo, Jie Zhou, Xunxun Chen, Yuhui Chen, Qinghua Liao, Yang Shu, Yaoju Tan, Meiling Yu, Guozhou Li, Lin Zhou, Qiu Zhong, Lijun Bi, Lina Guo, Meigui Zhao

“…TB-related cytokines and TB-specific antibodies were detected in parallel with microarray technology. The diagnostic performance of the new protocol for active TB was systematically compared with other traditional methods.…”
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Performance and clinical implications of non-invasive prenatal testing for rare chromosomal abnormalities: a retrospective study of 94,125 cases by Haimei Qi, Haijun Chen, Zhuling Zhang, Jinhui Gan, Huifeng Liu, Xianping Yuan, Fuyu Luo, Junkun Chen, Shichun Shen

“…BackgroundNon-invasive prenatal testing (NIPT) has demonstrated robust performance in detecting common trisomies and copy number variations. …”
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Integrative analysis of T cell-mediated tumor killing-related genes reveals KIF11 as a novel therapeutic target in esophageal squamous cell carcinoma by Xinxin Cheng, Huihui Zhao, Zhangwang Li, Liping Yan, Qingjie Min, Qingnan Wu, Qimin Zhan

“…The KIF11 expression was detected by multiplex fluorescent immunohistochemistry on tissue microarray from ESCC patients. …”
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Determination of Viral Nucleic Acid in the Human Blood by M. A. Abdurashitov, N. A. Netesova

“…Methods such as in situ hybridization, reverse transcription reaction (RT-PCR), nested PCR, multiplex PCR, as well as DNA microarray technology, and the method of massive parallel sequencing are considered in detail. …”
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Long read whole genome sequencing-based discovery of structural variants and their role in aetiology of non-syndromic autism spectrum disorder in India by Jhanvi Shah, Debasrija Mondal, Deepika Jain, Priti Mhatre, Ketan Patel, Anand Iyer, Manoj Pandya, Bhargavi Menghani, Gayatri Dave, Jayesh Sheth, Frenny Sheth, Shweta Ramdas, Harsh Sheth

“…Short read sequencing (SRS) and chromosomal microarray (CMA) are unable to resolve these SVs due to their inherent technological limitations. …”
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Sequential prenatal diagnosis of fetal skeletal dysplasia: A cohort study by Mengting Jiang, Bin Zhang, Jing Wang, Wei Qiao, Xiuzhen Mao, Bin Yu

“…Abstract Introduction Genetic factors are considered to be the main factors leading to fetal skeletal dysplasia (SD), and chromosomal microarray analysis (CMA) has been used clinically for the detection of SD fetuses. …”
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Nanopore-based random genomic sampling for intraoperative molecular diagnosis by Francesco E. Emiliani, Abdol Aziz Ould Ismail, Edward G. Hughes, Gregory J. Tsongalis, George J. Zanazzi, Chun-Chieh Lin

“…Results In our retrospective cohort of 26 malignant brain tumors, iSCORED demonstrated 100% concordance in CNV detection, including chromosomal alterations and oncogene amplifications, when compared to clinically validated assays such as Next-Generation Sequencing and Chromosomal Microarray. …”
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Over-expression of either MECP2_e1 or MECP2_e2 in neuronally differentiated cells results in different patterns of gene expression. by Marija Orlic-Milacic, Liana Kaufman, Anna Mikhailov, Aaron Y L Cheung, Huda Mahmood, James Ellis, Peter J Gianakopoulos, Berge A Minassian, John B Vincent

“…DOCK8 was shown via microarray and qRT-PCR to be upregulated in both SK-N-SH cells and mouse fibroblasts. …”
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Pan-cancer analysis identifies CD155 as a promising target for CAR-T cell therapy by Xiaohong Liu, Yue Sun, Boxu Lin, Hao Xiong, Xinyue Lu, Binghe Tan, Chenglin Zhang, Mingyao Liu, Juliang Qin, Na Zhang, Bing Du

“…Conclusions Our study demonstrates that CD155 is selectively expressed in cancer cells while being rarely detected in normal tissues, and may serve as a promising pan-cancer target for CAR-T therapy. …”
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Comparative linkage meta-analysis reveals regionally-distinct, disparate genetic architectures: application to bipolar disorder and schizophrenia. by Brady Tang, Tricia Thornton-Wells, Kathleen D Askland

“…Since it is likely a majority of common variants of modest effect have been identified through the application of tagSNP-based microarray platforms (i.e., GWAS), alternative approaches robust to detection of low-frequency (1-5% MAF) and rare (<1%) variants are of great importance. …”
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Concave Magnetic-Responsive Hydrogel Discs for Enhanced Bioassays by Amin Ghaffarzadeh Bakhshayesh, Huiyan Li

“…This approach enables immediate preparation and customization of hydrogel properties such as porosity, magnetic responsiveness, and embedded particles and is adaptable for use with microarray printers. The concave design increased the surface area by 43% compared to conventional hemispherical hydrogels, enhancing diffusion rates and accelerating reactions. …”
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On-Chip DNA Assembly via Dielectrophoresis by Xichuan Rui, Lin-Sheng Wu, Xin Zhao

“…This work introduces a novel on-chip DNA assembly method via dielectrophoresis (DEP) that can potentially be integrated with microarray-based oligo synthesis on the same chip. Our DEP chip can selectively manipulate oligos and guide their movement without perturbing the surrounding fluid medium, thus aiding in DNA assembly. …”
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SLEPR: a sample-level enrichment-based pathway ranking method -- seeking biological themes through pathway-level consistency. by Ming Yi, Robert M Stephens

“…Analysis of microarray and other high throughput data often involves identification of genes consistently up or down-regulated across samples as the first step in extraction of biological meaning. …”
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