Resistome and Phylogenomics of <i>Escherichia coli</i> Strains Obtained from Diverse Sources in Jimma, Ethiopia by Mulatu Gashaw, Esayas Kebede Gudina, Guenter Froeschl, Ralph Matar, Solomon Ali, Liegl Gabriele, Amelie Hohensee, Thomas Seeholzer, Arne Kroidl, Andreas Wieser

“…Isolates exhibiting phenotypic resistance to beta-lactam antibiotics were further analyzed with a DNA microarray to confirm the presence of resistance-encoding genes. …”
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Improved epigenetic age prediction models by combining sex chromosome and autosomal markers by Zhong Wan, Peter Henneman, Huub C. J. Hoefsloot, Ate D. Kloosterman, Pernette J. Verschure

“…Models constructed using a selected set of 37 X chromosomal and six autosomal DNAm markers exhibited a significantly improved age prediction performance with a minimum RMSE of 2.54 years and MAD of 1.89 years. …”
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Construction of a circRNA-miRNA-mRNA Regulatory Network for the Immune Regulation of Lung Adenocarcinoma by Hanyi Li, Xin Jin, Wei Li, Fan Ren, Tong Li, Xuanguang Li, Haochuan Yu, Dianxun Fu, Zuoqing Song, Song Xu

“…Methods and Materials We utilized microarray datasets from the GEO NCBI database (GSE101586) to identify differentially expressed circRNAs (DEcircRNAs) in LUAD. …”
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Spatially resolved analysis of the T cell immune contexture in lung cancer-associated brain metastases by Richa Gupta, Sarah B Goldberg, Kurt A Schalper, Harriet M Kluger, Lucia B Jilaveanu, Adebowale J Adeniran, Benjamin Y Lu, Adam Aguirre-Ducler, Nicole Gianino, Hailey Wyatt, Matthew Ribeiro, Veronica L Chiang, Joseph N Contessa

“…The signal for each marker was measured in marker-selected tissue compartments, and associations between marker levels, tumor location, and major clinicopathological variables were studied. …”
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Tumor immune microenvironment permissive to metastatic progression of ING4-deficient breast cancer. by Emily Tsutsumi, Anne M Macy, Janine LoBello, Karen T Hastings, Suwon Kim

“…To characterize the tumor immune microenvironment associated with ING4-deficient tumors, three approaches were employed in this study: First, tissue microarrays composed of 246 primary breast tumors including 97 ING4-deficient tumors were evaluated for the presence of selective immune markers, CD68, CD4, CD8, and PD-1, using immunohistochemical staining. …”
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Circular RNA APP contributes to Alzheimer’s disease pathogenesis by modulating microglial polarization via miR-1906/CLIC1 axis by Deng-Pan Wu, Yan-Su Wei, Li-Xiang Hou, Yu-Xuan Du, Qiu-Qing Yan, Ling-Ling Liu, Yuan-Dan Zhao, Ru-Yu Yan, Chao Yu, Zhen-Guo Zhong, Jin-Lan Huang

“…RT-PCR assay was conducted to detect the expression of circAPP, miR-1906 and CLIC1. …”
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Candidate gene biodosimetry markers of exposure to external ionizing radiation in human blood: A systematic review. by Jerome Lacombe, Chao Sima, Sally A Amundson, Frederic Zenhausern

“…Genes identified in at least half of the selected studies were retained for bio-statistical analysis in order to evaluate their diagnostic ability.…”
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CD226+ B cells in primary Sjögren’s syndrome: a key player in clinical manifestations and disease pathogenesis by Ping Zhao, Ping Zhao, Saizhe Song, Song Zhang, Cheng Peng, Wei Cheng, Xin Chang, Xin Chang, Changhao Xie, Zhongli Hu, Cuiping Liu

“…Multicolor immunofluorescence staining was applied to detect the co-expression of B cells and CD226 in the salivary gland of pSS patients.Microarray analysis was conducted to analyze the transcriptomic profiles of sorted CD226+ CD19+ B cells and CD226- CD19+ B cells.ResultsCD226 expression in the peripheral blood of pSS patients was significantly increased on T cells, CD19+ B cells and CD14+ monocytes, but significantly decreased on CD56+ NK cells.We identified a distinct CD226+CD19+ B cell subset that exhibited pathogenic features in pSS. …”
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Berberine Improves Inflammatory Responses of Diabetes Mellitus in Zucker Diabetic Fatty Rats and Insulin-Resistant HepG2 Cells through the PPM1B Pathway by Yang Sheng Wu, Zhe Ming Li, Yi Tao Chen, Shi Jie Dai, Xiao Jie Zhou, Yuan Xiao Yang, Jian Shu Lou, Li Ting Ji, Yu Ting Bao, Ling Xuan, Lu Ning Lin, Chang Yu Li

“…Microarray analysis of liver tissues was performed, and differentially expressed gene (DEG) levels were examined by quantitative real-time PCR (qPCR) and Western blot. …”
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Identification and external validation of tumor DNA methylation panel for the recurrence risk stratification of stage II colon cancer by Tanwei Yuan, Dominic Edelmann, Víctor Moreno, Elisabeth Georgii, Lisa Barros de Andrade e Sousa, Helena Pelin, Xiaofeng Jiang, Jakob Nikolas Kather, Katrin E. Tagscherer, Wilfried Roth, Melanie Bewerunge-Hudler, Alexander Brobeil, Matthias Kloor, Hendrik Bläker, Hermann Brenner, Michael Hoffmeister

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Disruption of bioenergetics enhances the radio-sensitivity of patient-derived glioblastoma tumorspheres by Seo Jin Kim, Junseong Park, Jin-kyoung Shim, Ran Joo Choi, Ju Hyung Moon, Eui Hyun Kim, Wan-Yee Teo, Jong Hee Chang, Seok-Gu Kang

“…Methods: Gene expression profiles of GBM patient-derived TSs were obtained through microarray and RNA-seq. In vitro treatment efficacy was assessed using clonogenic assay, 3D invasion assay, neurosphere formation assay, and flow cytometry. …”
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Autoantibodies Targeting Vinculin Reveal Novel Insight into the Mechanisms of Autoimmune Podocytopathies by Hanyan Meng, Dongjie Wang, Chen Zheng, Chao Zhou, Xinrui Mao, Jinglan Gu, Xiaohui Qiao, Fei Liu, Jingjing Wang, Haidong Fu, Jianhua Mao, Qing Ye

“…Methods: Serum anti-vinculin autoantibody levels detected by protein microarray and clinical data were compared among INS patients (n = 147), healthy individuals (n = 84), and patients with other kidney or immune diseases (n = 100 of each disease). …”
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Inhibitory Effect of PRMT5/MTA Inhibitor on MTAP‐Deficient Glioma May Be Influenced by Surrounding Normal Cells by Yunjie Wang, Xiaohui Sun, Runchen Ma, Xiaofan Zhang, Shengmin Ji, Zhaofeng Liu, Gangqiang Yang, Hongbo Wang, Peng Zhang, Jianzhao Zhang, Jingwei Tian

“…In vitro and in vivo experiments have demonstrated that MRTX1719, a small molecule that selectively binds PRMT5/MTA complex, significantly inhibits the proliferation of MTAP‐deficient tumors and has a weak toxic effect on normal cells. …”
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History and prospects of flax genetic markers by Daiana A. Zhernova, Elena N. Pushkova, Tatiana A. Rozhmina, Elena V. Borkhert, Alexander A. Arkhipov, Alexander A. Arkhipov, Elizaveta A. Sigova, Elizaveta A. Sigova, Ekaterina M. Dvorianinova, Ekaterina M. Dvorianinova, Alexey A. Dmitriev, Alexey A. Dmitriev, Nataliya V. Melnikova

“…Cost-effective and accurate analysis of large number of genotypes for multiple markers simultaneously using microarrays or targeted deep sequencing became available, as well as HRM, TaqMan, KASP, and other fluorescence-based high-throughput methods for detecting DNA polymorphisms. …”
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Long Noncoding RNA Expression Profiles of Periodontal Ligament Stem Cells from the Periodontitis Microenvironment in Response to Static Mechanical Strain by Jia Liu, Yan Zhao, Qiannan Niu, Ni Qiu, Shuangyun Liu, Chunrong Li, Cuixia Li, Pei Miao, Libo Yan, Qiang Li, Zuolin Jin

“…First, we subjected PDLSCs obtained from healthy periodontal tissues (HPDLSCs) and PPDLSCs to static mechanical strain (SMS) with 12% elongation at 0.1 Hz frequency using an FX-4000T system and screened overall lncRNA profiles in both cell types by microarray. Among lncRNAs with a fold change FC>20.0, 27 lncRNAs were upregulated in strained HPDLSCs, and 16 lncRNAs (9 upregulated and 7 downregulated) were detected in strained PPDLSCs. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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In vivo RNAi screen and validation reveals Ngp, Hba-a1, and S100a8 as novel inhibitory targets on T lymphocytes in liver cancer by Inga Hochnadel, Lisa Hoenicke, Nataliia Petriv, Huizhen Suo, Lothar Groebe, Chantal Olijnik, Nina Bondarenko, Nina Bondarenko, Juan C. Alfonso, Michael Jarek, Ruibing Shi, Andreas Jeron, Andreas Jeron, Kai Timrott, Tatjana Hirsch, Nils Jedicke, Dunja Bruder, Dunja Bruder, Frank Klawonn, Frank Klawonn, Frank Klawonn, Ralf Lichtinghagen, Robert Geffers, Henrike Lenzen, Henrike Lenzen, Michael P. Manns, Tetyana Yevsa

“…Therefore, in this study, we aimed to define novel ICI molecules arising on T cells during aggressive HCC development.MethodsUsing autochthonous HCC models, we performed microarray analyses followed by in vivo RNA interference screen and identified several new ICI molecules on CD4 and CD8 T lymphocytes in HCC-bearing mice. …”
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Molecular profiling analysis for colorectal cancer patients with Pi-Xu or Shi-Re syndrome by Xinbing Sui, Yong Guo, Wei Ni, Haiyan Jin, Haoming Lin, Tian Xie

“…The protein expression was determined by Immunohistochemistry (IHC) staining and mRNA profiling was detected by expression microarray. Furthermore, mRNA fold change was evaluated by qRT-PCR. …”
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Eurycomalactone switched hepatocellular carcinoma cells into quiescence through 5’tRFAla/DVL/β-catenin pathway inhibition by Zhipeng Zhang, Yanmei Wu, Wenqiang Liang, Zhifang Liao, Hongbo Liao, Xingxing Xing, Wenxin Yi, Zixuan Liu, Yicheng Li, Mengya Shi, Dongling Lin, Ting Gu, Biao Wu, Mingzhi Zou, Huilai Miao, Xin Wu

“…Subsequently, two new tsRNAs, namely 5’tRFAla and 5’tiRNAAla, which match well with the mRNAs of two pivotal upstream regulators (DVL2 and DVL3) of β-catenin based on bioinformatics analyses, were detected to be significantly decreased in ELT-treated PLC/PRF/5 cells using Arraystar small RNA microarray analyses. …”
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MiR-145-5p arrests the cell cycle by modulating SMAD5/cyclin D1 to inhibit gastric cancer progression by Li Wang, Li Wang, Jianghong Guo, Caixia Lv, Luke Kong, Luke Kong, Jing Cui, Zhongshuai Wang, Yuanyuan Guo, Ruirui Jia, Tao Guan, Tao Guan, Baofeng Yu, Feng Li

“…PurposeThis study aimed to explore the dysregulation of miR-145-5p in gastric cancer (GC) and its effects on the proliferation and cell cycle of GC cells, exploring the potential regulatory mechanism of miR-145-5p in GC.MethodsIn this study, the TCGA database combined with Microarray was used to detect differentially expressed microRNA (miRNA) in GC tissues and cells. …”
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