IGFBP7 promotes the proliferation and differentiation of primary myoblasts and intramuscular preadipocytes in chicken by Wanzhuo Geng, Yulong Guo, Botong Chen, Xi Cheng, Shuohan Li, Mohammed Kamal Challioui, Weihua Tian, Hong Li, Yanhua Zhang, Zhuanjian Li, Ruirui Jiang, Yadong Tian, Xiangtao Kang, Xiaojun Liu

“…We then examined the expression patterns of IGFBP7 across different stages of proliferation and differentiation in CPMs and intramuscular preadipocytes via qPCR, and explored the biological functions of IGFBP7 through gain- and loss-of-function experiments and a range of techniques including qPCR, CCK-8, EdU, flow cytometry, Western blot, immunofluorescence, and Oil Red O staining to detect the proliferation, differentiation, and lipid deposition in CPMs and intramuscular preadipocytes. …”
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Research note: Harnessing the potential of chicken blastodermal cells as a new frontier in poultry science and biotechnology – Establishment of embryonic stem cells and their diffe... by Małgorzata Duda, Patrycja Maciak, Grzegorz Tylko, Kamil Wartalski, Monika Trzcińska, Marek Romek, Marcin Samiec

“…These findings highlight the potential of B-ESCs as a valuable source for propagating SC-LCs, with implications for regenerative medicine and neural/glial tissue engineering applications. …”
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Targeted proteomics for absolute quantification of glucose transporter 1 in mammalian brain cells using liquid chromatography-mass spectrometry by Yash Mehta, Dhavalkumar Patel, Iqra Pervaiz, Ulrich Bickel, Abraham Jacob Al-Ahmad

“…Glucose represents a major source of energy for mammalian brains. Its uptake is tributary to a functional glucose transporter (GLUT1) expression at the blood-brain barrier (BBB). …”
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Distinct microbial communities drive methane cycling in below- and above-ground compartments of tropical cloud forests growing on peat by Fahad Ali Kazmi, Ülo Mander, Ramita Khanongnuch, Maarja Öpik, Reti Ranniku, Kaido Soosaar, Mohit Masta, Salla A. M. Tenhovirta, Kuno Kasak, Claudine Ah-Peng, Mikk Espenberg

“…However, no CH4-cycling functional genes were detected in the stem core samples. …”
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Generation of Cas9 Knock-In <i>Culex quinquefasciatus</i> Mosquito Cells by Elizabeth Walsh, Tran Zen B. Torres, Brian C. Prince, Claudia Rückert

“…In comparison to the RNAi-mediated gene silencing of <i>dicer-2</i>, <i>argonaute-2b</i>, and <i>piwi5</i>, our Cas9/sgRNA approach showed an enhanced ability to detect antiviral effects. <b>Conclusions</b>: We propose that this cell line offers a new tool for studying gene function in <i>Cx. quinquefasciatus</i> mosquitoes that avoids the use of RNAi. …”
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Leveraging artificial intelligence for photo identification to aid CITES enforcement in combating illegal trade of the endangered humphead wrasse (Cheilinus undulatus) by C. Y. Hau, W. K. Ngan, Y. Sadovy de Mitcheson, Y. Sadovy de Mitcheson, Y. Sadovy de Mitcheson

“…A Licence to Possess system for CITES is implemented in the city to regulate the quota of live wild-sourced CITES specimens, including humphead wrasse, held at each registered trading premise and ensure traceability through documentation. …”
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Chromosome-level genome assembly of Phortica okadai, a vector of Thelazia callipaeda by Lingjun Wang, Hongsong Yu, Bo Luo, Rong Yan, Juan Zhou, Hui Liu, Ze Zhang, Wei Sun

“…Additionally, 302 rRNAs, 377 tRNAs, 52 miRNAs, 40 snRNAs and 21 snoRNAs were detected. In conclusion, this high-quality chromosome level reference genome will be a valuable resource for furthering the fields of ecology, genetics, evolution and functional genomics of P. okadai.…”
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Optimisation of Ultrasound-Assisted Extraction Conditions Using Response Surface Methodology and Identification of Thymoquinone from Black Cumin (Nigella sativa L.) Seed Extract§ by Nita Kaushik, Aradhita Barmanray

“…The presence of thymoquinone was further confirmed by the functional groups detected in FTIR analysis. Under the specified extraction conditions, total phenolic content (TPC, expressed as gallic acid equivalents), yield (in %) and DPPH radical scavenging activity increased by approx. 271.03 mg/g, and 4.5 and 83.06 %, respectively. …”
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Glowing Green: A Quantitative Analysis of Photoluminescence in Six North American Bat Species by Briana J. Roberson, Santiago Perea, Daniel DeRose‐Broeckert, Steven B. Castleberry

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Hybrid closed-loop systems for managing blood glucose levels in type 1 diabetes: a systematic review and economic modelling by Asra Asgharzadeh, Mubarak Patel, Martin Connock, Sara Damery, Iman Ghosh, Mary Jordan, Karoline Freeman, Anna Brown, Rachel Court, Sharin Baldwin, Fatai Ogunlayi, Chris Stinton, Ewen Cummins, Lena Al-Khudairy

“…These improve the cost-effectiveness of HCL to £163,000 per QALY if it is valued using the EAG preferred source, to £121,000 if it is valued using the same source as NG17 and to £109,000 if it is valued using other credible sources. …”
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Genome-Wide Responses of Female Fruit Flies Subjected to Divergent Mating Regimes. by Dave T Gerrard, Claudia Fricke, Dominic A Edward, Dylan R Edwards, Tracey Chapman

“…However, the subtle nature of those effects suggests substantial functional redundancy or parallelism in this gene network, which could buffer females against excessive responses. …”
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IT GOVERNANCE IN THE BANKING SECTOR: EVIDENCE FROM ITALY, GERMANY, FRANCE AND SPAIN by Ida Claudia Panetta, Sabrina Leo, Fabrizio Santoboni, Gianfranco Vento

“…IT is an intrinsic component of banks’ operational functioning; it is a key resource in developing and supporting banking services, enabling institutions’ strategies, and it is essential for almost all banking processes and distribution channels. …”
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Rapid cohort generation and analysis of disease spectrum of large animal model of cone dystrophy. by Corinne Kostic, Simon Geoffrey Lillico, Sylvain Vincent Crippa, Nicolas Grandchamp, Héloïse Pilet, Stéphanie Philippe, Zen Lu, Tim James King, Jacques Mallet, Chamsy Sarkis, Yvan Arsenijevic, Christopher Bruce Alexander Whitelaw

“…Sixty percent of the piglets were transgenic, with mutant GUCY2D mRNA detected in the retina of all animals tested. Functional impairment of vision was observed among the transgenic pigs at 3 months of age, with a follow-up at 1 year indicating a subsequent slower progression of phenotype. …”
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Soil pollution and remediation: emerging challenges and innovations by Hao Chen, Bin Gao, Yuncong Li

“…These pollutants pose severe risks to environmental health and agricultural productivity by altering soil functionality and contaminant mobility. This perspective summarizes innovative monitoring and remediation technologies, including advanced sensors and bioremediation strategies, that enable real-time detection and effective management of soil pollutants. …”
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In vitro fermentation of whole matrix, digested products and β-glucan enriched extract of Pleurotus eryngii mushrooms distinctively impact the fecal microbiota of healthy older adu... by Evangelia N. Kerezoudi, Marigoula Vlassopoulou, Evdokia K. Mitsou, Georgia Saxami, Georgios Koutrotsios, Ioanna Taflampa, Konstantinos C. Mountzouris, Ignacio Rangel, Robert J. Brummer, Georgios I. Zervakis, Vasiliki Pletsa, Panagiotis Georgiadis, Adamantini Kyriacou

“…The changes in bacterial communities upon fermentation at family, genera and species level were detected via 16S rRNA Next Generation Sequencing and Quantitative real-time PCR. …”
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Identification of Genomic Variants and Candidate Genes for Reproductive Traits and Growth Traits in Pishan Red Sheep Using Whole-Genome Resequencing by Maimaitijiang Muhetapa, Mengting Zhu, Aladaer Qi, Sulaiman Yiming

“…Through selective sweep analysis, 92 genomic regions under selection were detected, containing 90 positional candidate genes significantly associated with growth, reproduction, and immune functions. …”
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Genome-wide association mapping identifies a new arsenate reductase enzyme critical for limiting arsenic accumulation in plants. by Dai-Yin Chao, Yi Chen, Jiugeng Chen, Shulin Shi, Ziru Chen, Chengcheng Wang, John M Danku, Fang-Jie Zhao, David E Salt

“…Complementation verified the identity of HAC1, and expression in Escherichia coli lacking a functional arsenate reductase confirmed the arsenate reductase activity of HAC1. …”
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Diversity and composition of sponge-associated microbiomes from Korean sponges revealed by full-length 16S rRNA analysis by Myoung-Sook Shin, Inho Yang, Weihong Wang, Hiyoung Kim

“…Our integrative, high-resolution approach not only uncovers remarkable taxonomic and functional diversity, but also provides a valuable genetic resource for future marine natural-product discovery and advances ecological restoration efforts.…”
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Pulmonary complications in children and adolescents after allogeneic hematopoietic stem cell transplantation by A. S. Frolova, A. G. Volkova, O. V. Paina, P. V. Kozhokar, K. A. Ekushov, Zh. Z. Rakhmanova, L. A. Cvetkova, E. D. Dobrovolskaya, B. I. Smirnov, E. A. Kulagin, T. A. Bykova, E. V. Semenova, L. S. Zubarovskaya

“…However to date, the effect of HSCT on functional and morphological pulmonary changes in recipients remains insufficiently studied.The objective of current study was to evaluate risk factors affecting long-term survival in children and adolescents after allo-HSCT.Methods and materials. …”
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The amino acid composition of Polygonum hydropiper L. and Polygonum persicaria L. herbs of Ukrainian flora by I. A. Lukina, O. V. Mazulin, H. P. Smoilovska, H. V. Mazulin, I. N. Shevchenko

“…The received results testify the promising use of the plant material and will be considered in the future work for the more in-depth study of the selected objects as a source of natural bioactive substances.…”
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