Generation of Cas9 Knock-In <i>Culex quinquefasciatus</i> Mosquito Cells by Elizabeth Walsh, Tran Zen B. Torres, Brian C. Prince, Claudia Rückert

“…In comparison to the RNAi-mediated gene silencing of <i>dicer-2</i>, <i>argonaute-2b</i>, and <i>piwi5</i>, our Cas9/sgRNA approach showed an enhanced ability to detect antiviral effects. <b>Conclusions</b>: We propose that this cell line offers a new tool for studying gene function in <i>Cx. quinquefasciatus</i> mosquitoes that avoids the use of RNAi. …”
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Chromosome-level genome assembly of Phortica okadai, a vector of Thelazia callipaeda by Lingjun Wang, Hongsong Yu, Bo Luo, Rong Yan, Juan Zhou, Hui Liu, Ze Zhang, Wei Sun

“…Additionally, 302 rRNAs, 377 tRNAs, 52 miRNAs, 40 snRNAs and 21 snoRNAs were detected. In conclusion, this high-quality chromosome level reference genome will be a valuable resource for furthering the fields of ecology, genetics, evolution and functional genomics of P. okadai.…”
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Optimization of Ultrasonic-Enzymatic-Assisted Extraction of Flavonoids from Sea Buckthorn (<i>Hippophae rhamnoides</i> L.) Pomace: Chemical Composition and Biological Activities by Wenyu Suo, Wenzhe Wang, Dajing Li, Haihong Wu, Haiyan Liu, Wuyang Huang, Yanhong Ma

“…Sea buckthorn pomace (SBP) is a rich source of flavonoid compounds with potential healthy properties. …”
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Characterize the Complete Mitogenome of Semiaquilegia guangxiensis and Assess the Efficiency of the Mitochondrial Genes in Ranunculales Phylogeny by Zheng‐Juan Zhu, Xin‐Mei Qin, Peng‐Wei Li, Yong‐Bin Lu, Xiao‐Yuan Mo, Yuan Fang, Qiang Zhang

“…Moreover, a large number of repetitive mitogenome sequences and chloroplast‐sourced sequences transferred to the mitogenome were detected. …”
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Glowing Green: A Quantitative Analysis of Photoluminescence in Six North American Bat Species by Briana J. Roberson, Santiago Perea, Daniel DeRose‐Broeckert, Steven B. Castleberry

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The Effect of Hearing Aid Amplification on Gait Parameters: A Pilot Study Using Ear-Worn Motion Sensors by Ann-Kristin Seifer, Arne Küderle, Kaja Strobel, Ronny Hannemann, Björn M. Eskofier

“…Additionally, we showed the potential of ear-worn sensors for detecting relevant gait changes. To achieve this, we used a hearing-aid-integrated accelerometer and our open-source EarGait framework comprising gait-related algorithms specifically developed for ear-worn sensors. …”
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Compact and Handheld SiPM-Based Gamma Camera for Radio-Guided Surgery and Medical Imaging by Fabio Acerbi, Aramis Raiola, Cyril Alispach, Hossein Arabi, Habib Zaidi, Alberto Gola, Domenico Della Volpe

“…We characterized the intrinsic position resolution (tested with pulsed light) as being ~200 µm, and the sensitivity and resolution when detecting gamma rays from Tc-99m source measured between 134 and 481 cps/MBq and as good as 1.4–1.9 mm, respectively.…”
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Identification of Soybean E1-E4 Gene Orthologs in the Guar Genome Using Comprehensive Transcriptome Assembly and Annotation by Luisa Criollo Delgado, Aleksei Zamalutdinov, Elena Potokina

“…Taub.), a well-known source of guar gum (food additive E 412). At high latitudes, e.g., in Russia, the main challenge for guar cultivation is the long photoperiod during summer, which delays flowering and maturation of guar plants. …”
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Vitamin C, Sugar Content, Color Intensity and Some Physicochemical Properties of Watermelon and Orange Peels by Selvican Gökçe, İsa Arslan Karakütük, Memnune Şengül

“…Therefore, orange peel can be used as a natural antioxidant and color source in the food, pharmaceutical, and cosmetic industries.…”
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Wind Turbine Static Errors Related to Yaw, Pitch or Anemometer Apparatus: Guidelines for the Diagnosis and Related Performance Assessment by Davide Astolfi, Silvia Iuliano, Antony Vasile, Marco Pasetti, Salvatore Dello Iacono, Alfredo Vaccaro

“…Nevertheless, methods based on additional information sources (like further sensors or meteorological data) are also discussed. …”
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Engineered hybrid cell membrane nanosystems for treating cardiovascular diseases by He Lu, Yaohui Jiang, Rui Luo, Dexing Zhou, Feihu Zheng, Liran Shi, He Zhang, Yong Wang, Xiaodong Xu, Renfang Zou, Yujing Zhou, Shuai Ren, Xiaocheng Wang, Haiqiang Sang

“…A significant benefit associated with this technology lies in its capacity to retain the functional and intrinsic properties of the source cells. …”
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Comprehensive Phenotypic Characterization and Genomic Analysis Unveil the Probiotic Potential of <i>Bacillus velezensis</i> K12 by Yingying Tang, Tian Li, Yihong Huang, Liangliang Wu, Xiaobo Liu, Ruichao Yue, Jianmin Yuan

“…The complete genome sequencing of <i>B. velezensis</i> K12 revealed a genomic length of 3,973,105 base pairs containing 4123 coding genes, among which 3973 genes were functionally annotated. The genomic analysis identified genes associated with acid and bile tolerance, adhesion, antioxidants, and secondary metabolite production, whereas no functional genes related to enterotoxins or transferable antibiotic resistance were detected, thereby confirming the probiotic properties of <i>B. velezensis</i> K12. …”
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Genome-Wide Responses of Female Fruit Flies Subjected to Divergent Mating Regimes. by Dave T Gerrard, Claudia Fricke, Dominic A Edward, Dylan R Edwards, Tracey Chapman

“…However, the subtle nature of those effects suggests substantial functional redundancy or parallelism in this gene network, which could buffer females against excessive responses. …”
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IT GOVERNANCE IN THE BANKING SECTOR: EVIDENCE FROM ITALY, GERMANY, FRANCE AND SPAIN by Ida Claudia Panetta, Sabrina Leo, Fabrizio Santoboni, Gianfranco Vento

“…IT is an intrinsic component of banks’ operational functioning; it is a key resource in developing and supporting banking services, enabling institutions’ strategies, and it is essential for almost all banking processes and distribution channels. …”
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Rapid cohort generation and analysis of disease spectrum of large animal model of cone dystrophy. by Corinne Kostic, Simon Geoffrey Lillico, Sylvain Vincent Crippa, Nicolas Grandchamp, Héloïse Pilet, Stéphanie Philippe, Zen Lu, Tim James King, Jacques Mallet, Chamsy Sarkis, Yvan Arsenijevic, Christopher Bruce Alexander Whitelaw

“…Sixty percent of the piglets were transgenic, with mutant GUCY2D mRNA detected in the retina of all animals tested. Functional impairment of vision was observed among the transgenic pigs at 3 months of age, with a follow-up at 1 year indicating a subsequent slower progression of phenotype. …”
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Soil pollution and remediation: emerging challenges and innovations by Hao Chen, Bin Gao, Yuncong Li

“…These pollutants pose severe risks to environmental health and agricultural productivity by altering soil functionality and contaminant mobility. This perspective summarizes innovative monitoring and remediation technologies, including advanced sensors and bioremediation strategies, that enable real-time detection and effective management of soil pollutants. …”
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Identification of Genomic Variants and Candidate Genes for Reproductive Traits and Growth Traits in Pishan Red Sheep Using Whole-Genome Resequencing by Maimaitijiang Muhetapa, Mengting Zhu, Aladaer Qi, Sulaiman Yiming

“…Through selective sweep analysis, 92 genomic regions under selection were detected, containing 90 positional candidate genes significantly associated with growth, reproduction, and immune functions. …”
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Genome-wide association mapping identifies a new arsenate reductase enzyme critical for limiting arsenic accumulation in plants. by Dai-Yin Chao, Yi Chen, Jiugeng Chen, Shulin Shi, Ziru Chen, Chengcheng Wang, John M Danku, Fang-Jie Zhao, David E Salt

“…Complementation verified the identity of HAC1, and expression in Escherichia coli lacking a functional arsenate reductase confirmed the arsenate reductase activity of HAC1. …”
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Diversity and composition of sponge-associated microbiomes from Korean sponges revealed by full-length 16S rRNA analysis by Myoung-Sook Shin, Inho Yang, Weihong Wang, Hiyoung Kim

“…Our integrative, high-resolution approach not only uncovers remarkable taxonomic and functional diversity, but also provides a valuable genetic resource for future marine natural-product discovery and advances ecological restoration efforts.…”
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Oxidative Stress Markers and C-Reactive Protein Are Related to Severity of Heart Failure in Patients with Dilated Cardiomyopathy by Celina Wojciechowska, Ewa Romuk, Andrzej Tomasik, Bronisława Skrzep-Poloczek, Ewa Nowalany-Kozielska, Ewa Birkner, Wojciech Jacheć

“…The aim of study was to determine relationships between functional capacity (NYHA class), left ventricle ejection fraction (LVEF), hemodynamic parameters, and biomarkers of redox state and inflammation in patients with dilated cardiomyopathy (DCM). …”
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