Interdisciplinary medical education practices: building a case-driven interdisciplinary simulation system based on public datasets by Kangli Qiu, Tianshu Zeng, Wenfang Xia, Miaomiao Peng, Wen Kong

“…This creates a closed-loop practice that spans data sample selection and processing to front-end interaction. …”
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Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat by Tushadri Singh, Tushadri Singh, H. M. Mamrutha, Rajender Singh, J. P. Jaiswal, Zeenat Wadhwa, Rakesh Kumar, Omvir Singh, O. P. Ahlawat, Ratan Tiwari

“…By virtue of wheat being a hexaploid crop and having a large genome size with repetitive DNA, a tailor-made strategy for designing the gRNA is crucial.ResultThe manuscript explains the comprehensive strategies and methods for efficient gRNA designing by considering the physical and structural expression of the target gene in the genome and explains the on-target and off-target effects of gRNA for its precise editing through the CRISPR/Cas9-mediated SDN1 method of genome editing in wheat.ConclusionThe present manuscript is first of its kind to address the holistic approach, starting from efficient gene selection, gRNA designing, and post-gRNA designing issues like gRNA stability, binding efficiency, and functionality for SDN1-CRISPR/Cas9 genome editing in wheat. …”
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Fluxgate Magnetometers Based on New Physical Principles by Ivan V. Bryakin, Igor V. Bochkarev, Vadim R. Khramshin, Vadim R. Gasiyarov, Ivan N. Erdakov

“…The authors considered a generalized flow of the physical processes in the FM to obtain a detailed representation of the operating functions of the FM. The presented experimental results for the proposed FM in the field meter mode confirm its operating parameters (±40 μT—measurement range, 0.5 nT—detection threshold). …”
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An Optimization Algorithm for Forward-Scatter Radar Network Node Deployment Based on BFGS and Improved NSGA-II by Yuqing Zheng, Xiaofeng Ai, Zhiming Xu, Jing Wu, Feng Zhao

“…A multi-objective optimization model was subsequently established with the objective functions of minimizing node cost and maximizing spatial detection area. …”
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PRIDE-FTG advances academic careers of underrepresented early-stage investigators performing blood disorders research by Betty S. Pace, Oluwatoyosi A. Onwuemene, Athena Starlard-Davenport, Rhea-Beth Markowitz, Vahé Heboyan, Petar Datsov, Treva Rice

“…The selection process involved a rigorous review of applications, considering factors such as academic potential, research interests, and commitment to diversity in biomedical research. …”
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Wastewater metagenomics in Africa: Opportunities and challenges. by Stephen Kanyerezi, Fatma Zahra Guerfali, Abbas Abel Anzaku, Oluwasegun Adesina Babaleye, Tracey Calvert-Joshua, Julien Alban Nguinkal, Oluwaseun Paul Amoo, Chiraz Atri, Waqasuddin Khan, Iqra Saleh, M Imran Nisar, Arthur Shem Kasambula, Koketso Morapedi, Gerald Mboowa

“…The advent of metagenomics has dramatically expanded our understanding of microbial communities, particularly through the study of wastewater, which serves as a rich source of microbial data. In Africa, wastewater metagenomics presents unparalleled opportunities for public health monitoring, antimicrobial resistance (AMR) tracking, and the discovery of new microbial species and functions. …”
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Implementation of Diagnostic Methods of Career Guidance in Computer Systems by Anna P. Sergushicheva

“…As a result of crossing a pair of individuals from the parent population, a descendant is obtained whose chromosome consists of the genes of both parents. The selection of surviving specimens is based on the percentage of success in the development of each of the professions in the list and the fitness function. …”
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Ideal teacher of higher school: Personal qualities and socio-professional competencies by G. Z. Efimova, A. N. Sorokin, M. V. Gribovskiy

“…The increasing pace of knowledge renewal around the world and the unification of teacher requirements are changing the functioning and management of the higher education system. …”
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Progress Analysis and Development Prospects of Soil Ecological and Environmental Effects of Interlayer Construction by JIA Zhenjiang, WU Yangyang, LI Wangcheng, MA Dongxiang, CHEN Jihong, GAO Susu, NIU Xiaoxiao, XU Tianyuan

“…[Results] Interlayer construction could improve soil structure, nutrient content, ecosystem service functions, and agricultural production efficiency, but the responses process to interlayer mode selection and farmland management measures have shown differences. …”
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Elective staged proctocolectomy and living donor liver transplantation for colon cancer with sclerosing cholangitis-related ulcerative colitis: a case report by Yoshihiro Miyagi, Tatsuya Kinjo, Tomoharu Yoshizumi, Noboru Harada, Shingo Arakaki, Tetsu Kinjo, Akira Hokama, Mitsuhisa Takatsuki

“…To our knowledge, this is the first report of concurrent cancer successfully treated with both LDLT and proctocolectomy. …”
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Chemometrically assisted optimization and validation of the HPLC method for the analysis of alectinib and its related impurity with measurement uncertainty evaluation and whiteness... by Serkan Levent, Abeer Elriş, Hazal Avcı, Saniye Özcan, Nafiz Öncü Can

“…The method’s optimization was carried out using the Box-Behnken design, whereas the optimized conditions were selected according to the desirability function. Validation of the method was done with the International Council for Harmonization, ICH Q2(R2) Guidelines. …”
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Implementation of a Neural Network for Adaptive PID Tuning in a High-Temperature Thermal System by Juan Carlos Almachi, Ramiro Vicente, Edwin Bone, Jessica Montenegro, Edgar Cando, Salvatore Reina

“…Implemented on an 18 kW retrofitted Blue-M furnace, the system was characterized by second-order transfer functions for heating and forced convection cooling. …”
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Partial Purification of <i>Bacillus cereus</i> Enzyme Expression for Bio-Pulping of Lignin Degraders Isolated from <i>Coptotermus curvignathus</i> by Sharfina Mutia Syarifah, Ashuvila Mohd Aripin, Nadiah Ishak, Nosa Septiana Anindita, Mohd Firdaus Abdul-Wahab, Angzzas Sari Mohd Kassim

“…Despite extensive research on <i>Bacillus</i> sp. as lignin degraders, the enzyme mechanisms involved, particularly in <i>Bacillus cereus</i> isolated from termite guts, remain unclear. In this study, the selected Bacillus cereus was fermented to extract the lignin-degrading enzymes to identify the enzymes responsible for lignin degradation using the sample substrate empty fruit bunch (EFB) as their sole carbon source. …”
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The TRPA1 cation channel is upregulated by cigarette smoke in mouse and human macrophages modulating lung inflammation by Anita Steib, Katalin Rozmer, Éva Szőke, József Kun, Nelli Farkas, Diána Feller, Judit Pongrácz, Krisztina Pohóczky, Zsuzsanna Helyes

“…The transient receptor potential ankyrin 1 (TRPA1), a smoking-responsive, non-selective cation channel, is expressed by both capsaicin-sensitive peptidergic sensory nerves and non-neuronal cells of the lung, but there are few and controversial data on its expression and function on macrophages. …”
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The TOR kinase pathway is relevant for nitrogen signaling and antagonism of the mycoparasite Trichoderma atroviride. by Rossana Segreto, Hoda Bazafkan, Julia Millinger, Martina Schenk, Lea Atanasova, Maria Doppler, Christoph Büschl, Mélanie Boeckstaens, Silvia Soto Diaz, Ulrike Schreiner, Fabiano Sillo, Raffaella Balestrini, Rainer Schuhmacher, Susanne Zeilinger

“…Here we describe how alteration of the activity of TOR1, the single and essential TOR kinase of T. atroviride, by treatment with chemical TOR inhibitors or by genetic manipulation of selected TOR pathway components affected various cellular functions. …”
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Utilization of Garcinia mangostana L. Peel as an Immunomodulator to Improve the Quality of Human Resources: A Systematic Review by Yunita Satya Pratiwi, Dina Mustika Rini, Ifwarisan Defri, Tawaffani Qubra, Nadien Mutia Intan Maulidi

“…Background: Mangosteen (Garcinia mangostana L.) is recognized for its immunomodulatory properties, which can rejuvenate, regulate, and modulate immune system function. As a rich source of antioxidants, mangosteen peel has the potential to enhance immune responses and improve overall health status. …”
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Transcriptional profiling of nitrogen fixation and the role of NifA in the diazotrophic endophyte Azoarcus sp. strain BH72. by Abhijit Sarkar, Barbara Reinhold-Hurek

“…Moreover, the NifA regulon in strain BH72 appears to encompass a wider range of cellular functions beyond the regulation of nif genes.…”
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Range Space and Similarity Transformation Using Symbolic Mathematics in Python by Chainarong Wisassakwichai, Prasert Phaochoo

“…The effectiveness of these classes has been validated through numerical results, which, when compared to MATLAB functions (null(), pinv(), sym(), and jordan()), demonstrate that the exact symbolic solutions from both classes significantly improve numerical precision for selected application examples from numerical algorithms, power systems and linear algebra. …”
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Comparative Analysis of Hydration Status and Microbial Quality of Tap Water Between Urban and Rural Settings in the Ashanti Region of Ghana by Marina A. Tandoh, Priscilla Owusu, Claire-Rush N. A. Nkrumah, Veronica Tawiah Annaful, Chris Y. Asare, Samuel Selorm Attu

“…Six taps were randomly selected at each site in triplicates, resulting in 36 water samples. …”
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Design and Testing of a Small-Scale Composting Facility for Sheep Manure Utilizing Aeration and Thermal Treatment by Jian Wang, Kailin Ren, Longfei Zhang

“…This unit, tailored for small farms and individual farmers, integrates critical functions such as ventilation, heating, and turning. …”
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