Pancreatic neuroendocrine tumor with complete replacement of the pancreas by serous cystic neoplasms in a patient with von Hippel-Lindau disease: a case report by Shimpei Maeda, Fuyuhiko Motoi, Shuhei Oana, Kyohei Ariake, Masamichi Mizuma, Takanori Morikawa, Hiroki Hayashi, Kei Nakagawa, Takashi Kamei, Takeshi Naitoh, Michiaki Unno

“…The combination of pancreatic neuroendocrine tumors and serous cystic neoplasms is relatively rare, and the surgical treatment of these lesions must consider both preservation of pancreatic function and oncological clearance. We report a patient with von Hippel-Lindau disease successfully treated with pancreas-sparing resection of a pancreatic neuroendocrine tumor where the pancreas had been completely replaced by serous cystic neoplasms, in which pancreatic function was preserved. …”
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Construction of TRIF-knockout NA and BHK-21 cell lines and effect on rabies virus proliferation efficiency by Weitao HUANG, Jiaqi LI, Yao PENG, Xiangping YIN, Xiaofeng GUO, Jun LUO

“…ResultThe recombinant plasmids pSpCas9-NA-TRIF-KO and pSpCas9-BHK-21-TRIF-KO for TRIF-knockout were successfully constructed, the TRIF-knockout NA and BHK-21 cell lines were screened and identified. …”
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GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia by Lai L, Wu H, Peng L, Zhang Z, Wu X, Zheng S, Su Z, Chu H

“…A potential underlying mechanism could be that the autophagy induced by AST increases eNOS activity.Conclusion: GelMA@LNP/AST enables complete revascularization in female mice after hind limb ischemia, thereby achieving limb preservation and restoring motor function. Given the good therapeutic potential of the GelMA@LNP/AST in revascularization, it may become an effective strategy for successfully salvaging limbs in cases of limb ischemia.Keywords: astilbin, endothelial cells, autophagy, eNOS, angiogenesis…”
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Linking soil microbial genomic features to forest-to-pasture conversion in the Amazon by Andressa M. Venturini, Júlia B. Gontijo, Louis Berrios, Jorge L. Mazza Rodrigues, Kabir G. Peay, Siu M. Tsai

“…These abundant members of the microbial communities diverged across forests and pastures in terms of taxonomic and functional traits. Forest conversion favors organisms with specific genomic features — increased GC content, genome size, and gene number — selecting for microorganisms that can thrive under altered conditions. …”
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Effects of sinomenine on PI3K/mTOR signaling pathway and mesangial hyperplasia in rats with adriamycin-induced nephropathy by Liu Yue, Li Peng-yuan, Zhang Qing-hua, Yan Dan

“…Objective To explore the effect of sinomenine on the signal pathway of phosphatidyl-3 kinase（PI3 K）/mammalian target of rapamycin（mTOR）and mesangial hyperplasia in renal tissue of rats with adriamycin-induced nephropathy and elucidate its protective mechanism.Methods A total of 50 SD rats were selected, 10 rats were randomly injected with normal saline via caudal vein as control group, the other 40 rats were injected with adriamycin 5 mg/kg via caudal vein for 7 days to prepare nephrotic syndrome（NS）model.After successful modeling, the rats were randomly divided into the groups of model（NS）,sinomenine low-dose（25 mg/kg）,medium-dose（50 mg/kg）and high-dose（100 mg/kg）（n=10 each）.Control and NS groups received normal saline through abdominal cavity and sinomenine groups were given corresponding doses of drugs once daily for 28 days.At 24 hours after the last administration, blood and urine samples of rats in each group were collected and rats sacrificed, renal tissue samples harvested, serum creatinine（Scr）and urea nitrogen（BUN）were measured by enzyme-linked immunosorbent assay（ELISA）;24-hour urine protein was detected by Coomassie brilliant blue staining; hematoxylin-eosin staining（HE）was utilized for analyzing the pathological changes of kidney and mesangial matrix index（M/G）;transmission electron microscopy for observing the thickening of glomerular basement membrane of kidney; Western blot for detecting the expressions of p-PI3 K/PI3 K,p-mTOR/mTOR,fibronectin（FN）and type IV collagen（COL4）in renal tissue.Results As compared with control group, there were pathological damages such as the increase of glomerular mesangial matrix, the expansion of renal tubule lumen and severe thickening of glomerular basement membrane.M/G and renal function indicators Scr, BUN,24-hour urine protein and renal tissue were elevated.The protein expressions of p-PI3 K,p-mTOR,FN and COL4 rose markedly in NS group（P<0.05）.As compared with NS group, sinomenine high/medium/low dose groups alleviated pathological damage such as glomerular basement membrane thickening, M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,expressions of mTOR,FN and COL4 proteins declined markedly in sinomenine low/medium/high-dose groups（P<0.05）.As compared with low-dose sinomenine group, glomerular basement membrane thickening was the least pathologically damaged and M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,p-mTOR,FN and COL4 declined markedly in sinomenine medium/high-dose group（P<0.05）.Conclusions Sinomenine may inhibit the activation of PI3 K/mTOR signaling pathway, reduce the proliferation of glomerular mesangial cells and delay the process of nephrotic syndrome.…”
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Canada goose fecal microbiota correlate with geography more than host‐associated factors by Joshua C. Gil, Heather R. Skeen, Celeste Cuellar, Sarah M. Hird

“…This work also suggests that there is a minimum geographic distance, likely associated with sufficient variation in habitat, climate, and local food sources, that must be reached before significant differences in the microbiota between two populations can be detected.…”
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Strain Monitoring of Vertical Axis Wind Turbine Tower Using Fiber Bragg Gratings by Bastien Van Esbeen, Valentin Manto, Damien Kinet, Corentin Guyot, Christophe Caucheteur

“…First, FBG strain sensors were glued vertically along the tower to investigate the sensors behavior as a function of their height. The maximum signal-to-noise ratio is obtained when FBGs are placed at the tower base. …”
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Integrating parental genomes to reduce reference bias and identify intramuscular fat genes in Qinchuan Black pigs by Guangquan Lv, Peiyu Yang, Ao Guo, Minghao Cao, Dong Li, Zhe Liu, Mingyu Wang, Jingchun Sun, Rongrong Ding, Taiyong Yu

“…Selection signal analysis based on the integrated parental genomic strategy successfully identified more differentiated windows and positively selected genes. …”
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Pollution Characteristics and Water Quality Status of Water Storage Pond in Xianyang City of Shaanxi Province by Zhou Fuyu, Gao Zhaoliang, Zhang Shuai, Li Yonghong, Sun Guanfang, Lou Yongcai, Ai Jianwei, Liu Wenbo

“…Treatment methods suitable for the rural environment should be selected according to the characteristics of water pollution.…”
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Computational exploration of global venoms for antimicrobial discovery with Venomics artificial intelligence by Changge Guan, Marcelo D. T. Torres, Sufen Li, Cesar de la Fuente-Nunez

“…From these, we identified 386 candidates that are structurally and functionally distinct from known antimicrobial peptides. …”
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Recent Advances and Challenges in the Production of Hydroxylated Natural Products Using Microorganisms by Chang Sun, Rumei Zeng, Tianpeng Chen, Yibing Yang, Yi Song, Qiang Li, Jie Cheng, Bingliang Liu

“…Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing a crucial role in improving the bioactivity, solubility, and stability of natural product molecules. …”
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The association between serum albumin–globulin ratio and overactive bladder in American adults: a cross-sectional study by Yuan-Zhuo Du, Jia-Hao Liu, Fu-Chun Zheng, Qiang Zhou, Ju Guo

“…However, despite its widely studied correlations in numerous health fields, the link between AGR and Overactive Bladder (OAB) is still not completely comprehended. Methods Data were sourced from the National Health and Nutrition Examination Survey (NHANES) database, selecting adult samples spanning from 2007 to 2018. …”
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Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology by Mattia Santoni, Giovanni Piccinini, Giovanni Piccinini, Giovanni Liguori, Maria Roberta Randi, Massimo Baroncini, Liliana Milani, Francesca Danesi

“…The optimized model successfully detected concentration-dependent oxidative stress responses to TiO₂ exposure, revealing cellular dysfunction preceding membrane damage.DiscussionThis improved co-culture system provides a better physiological platform for nanotoxicology studies. …”
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Environmental DNA as a tool for soil health monitoring and unveiling new ecological frontiers by Yuan Zhang, Weijun Lu, Kaihang Xing, Fen Guo, Qingping Du, Xinfei Zhang, Fan Zhang, Zongyao Qian, Feilong Li

“…We observed a rapid increase in the use of eDNA for soil health, with more than 700 publications on soil eDNA methods since 2001, and an annual growth rate of over 20% since 2017. eDNA has been successfully applied to detect a wide range of taxa in soil, including bacteria, fungi, and metazoans, with bacteria being the most frequently studied group, accounting for 43% of total publications. …”
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Foamy Melamine Resin–Silica Aerogel Composite-Derived Thermal Insulation Coating by Dongfang Wang, Yabin Ma, Yingjie Ma, Baolei Liu, Dewen Sun, Qianping Ran

“…The results of foamy structure analyses via scanning electron microscopy, micro-CT, and N₂ adsorption–desorption isotherms validate the successful generation of a micro-scale porous structure with the enhancement of the aerogel nano-scale solid particles at the wall as a stabilizer. …”
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Nod2-Nodosome in a Cell-Free System: Implications in Pathogenesis and Drug Discovery for Blau Syndrome and Early-Onset Sarcoidosis by Tomoyuki Iwasaki, Naoe Kaneko, Yuki Ito, Hiroyuki Takeda, Tatsuya Sawasaki, Toshio Heike, Kiyoshi Migita, Kazunaga Agematsu, Atsushi Kawakami, Shinnosuke Morikawa, Sho Mokuda, Mie Kurata, Junya Masumoto

“…Notably, the ALPHA signal between Nod2-WT and RICK was increased upon incubation with MDP, but not when incubated with the same concentrations, L-alanine, D-isoglutamic acid, or the MDP-D-isoform. Thus, we successfully developed Nod2-nodosome in a cell-free system reflecting its function in vivo, and it can be useful for screening Nod2-nodosome-targeted therapeutic molecules for BS/EOS and granulomatous inflammatory diseases.…”
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Experience of extended transtriceps approach in osteosynthesis of complex fractures of the distal metaepiphysis of the humerus by A. Yu. Sergyeev, A. M. Fain, A. Yu. Vasa, S. F. Gnetetsky, V. B. Bondarev

“…Evaluation of treatment results confirms that osteosynthesis using this access allows to reduce the time and trauma of the operation, to avoid complications associated with osteotomy of the olecranon, and to carry out successful postoperative rehabilitation, achieving good functional results.…”
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Hepatic epithelioid hemangioendothelioma, a rare liver tumor: a case report and review of the literature by Chao Deng, Yulong An, Yulong An, Jinli Liu, Jinli Liu, Chao Wang, Haoxian Gou, Jinpeng Zhang, Minghui Gu, Mengmeng Li, Tao Wang, Hao Luo

“…Multiple space-occupying lesions in the liver were incidentally detected through ultrasound imaging, despite the patient being asymptomatic. …”
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Genome and Pathogenicity Analysis of an NADC30-like PRRSV Strain in China’s Xinjiang Province by Honghuan Li, Wei Zhang, Yanjie Qiao, Wenxing Wang, Wenxiang Zhang, Yueli Wang, Jihai Yi, Huan Zhang, Zhongchen Ma, Chuangfu Chen

“…In this study, we successfully isolated a novel strain named XJ-Z5 from PRRSV-positive samples collected in Xinjiang province in 2022. …”
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Comprehensive Phenotypic Characterization and Genomic Analysis Unveil the Probiotic Potential of <i>Bacillus velezensis</i> K12 by Yingying Tang, Tian Li, Yihong Huang, Liangliang Wu, Xiaobo Liu, Ruichao Yue, Jianmin Yuan

“…The complete genome sequencing of <i>B. velezensis</i> K12 revealed a genomic length of 3,973,105 base pairs containing 4123 coding genes, among which 3973 genes were functionally annotated. The genomic analysis identified genes associated with acid and bile tolerance, adhesion, antioxidants, and secondary metabolite production, whereas no functional genes related to enterotoxins or transferable antibiotic resistance were detected, thereby confirming the probiotic properties of <i>B. velezensis</i> K12. …”
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