Cloning and Analysis of the Expansin Gene BdEXP1 from Migration Plant parasitic Nematode (Bursaphelenchus doui) by Kaiyang GUO, Shifeng LIN, Rengang WANG, Li LI, Jie ZHANG, Shizhou YU, Zili WANG, Guoqin LIU

“…【Conclusion】The BdEXP1 gene was successfully cloned, and was also found to be expressed within the nematode esophageal gland of B. doui. …”
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Morning versus Nocturnal Heart Rate and Heart Rate Variability Responses to Intensified Training in Recreational Runners by Olli-Pekka Nuuttila, Heikki Kyröläinen, Veli-Pekka Kokkonen, Arja Uusitalo

“…The participants recorded daily their nocturnal HR and HRV (the natural logarithm of the root mean square of successive differences; LnRMSSD) with a photoplethysmography-based wrist device and performed an orthostatic test (2-min supine, 2-min standing) every morning with a chest-strap HR sensor. …”
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Establishment and characterization of liver‐specific Apoa4‐Cre and Cyp2c11‐Cre rat models in juvenile and adult stages by Wei Dong, Xiang Gao, Feifei Guan, Jirong Pan, Wei Chen, Li Zhang, Lianfeng Zhang

“…No histological alterations were detected in the livers of the Cre rats. Conclusions Liver‐specific Apoa4‐Cre and Cyp2c11‐Cre rats have been established successfully and could be used to study gene knockout, specifically in juvenile and adult liver.…”
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Evolution of Innovative Surgical Techniques Using the Example of Selective Splenorenal Anastomosis Surgery by A. Yu. Anisimov, F. B. Mavlonov, D. E. Erkinova, A. I. Andreev, R. A. Ibragimov, E. Yu. Mandryka

“…RELEVANCE Despite the obvious successes of transjugular intrahepatic portosystemic shunt and orthotopic liver transplantation in some high-tech centers of the Russian Federation, the relevance of substantiating the use of selective splenorenal anastomoses in the treatment plan for portal hypertension, when hundreds of patients return to emergency surgery departments every year after conservative treatment with new episodes of bleeding, liver failure and high mortality rates, is beyond doubt.AIM OF THE STUDY To substantiate the viability of the selective splenorenal anastomosis operation by improving the technique of performing the intraoperative vascular maneuver exposure and venous reconstruction through the use of original modern innovative approaches.MATERIAL AND METHODS A retrospective controlled clinical study included 56 patients with intrahepatic portal hypertension with relatively preserved liver function, in whose complex treatment program for secondary prevention of bleeding, various options for selective splenorenal bypass surgery were used. …”
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A compound heterozygous mutation in ZP1 and two novel heterozygous cis mutations in ZP3 causes infertility in women presenting with empty follicle syndrome by Xiaoxiao Wang, Yingxue Liu, Guanghui Yuan, Jingjie Yang, Xiaowen Liu, Shuyuan Chen, Huaiqian Dou, Panpan Lu, Linfang Han, Duan Li, Cuifang Hao

“…ELISA and Co-IP were used to detect the effects of variants on protein secretion and interactions. …”
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Alternative splicing in ovarian cancer by Liwei Wei, Yisheng Li, Jiawang Chen, Yuanmei Wang, Jianmin Wu, Huanming Yang, Yi Zhang

“…Abstract Ovarian cancer is the second leading cause of gynecologic cancer death worldwide, with only 20% of cases detected early due to its elusive nature, limiting successful treatment. …”
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Transcriptomic, phenotypic and physiological analyses identify candidate regulators of Dendrobium stigma maturation and pollen-stigma interaction by Qian Wu, Shuxian Ren, Yuehong Ruan, Jiaxue Hu, Yin Li, Zongyan Li

“…The stigma, as the primary site for pollen interaction, undergoes dynamic developmental changes that lay the foundation for successful pollination. However, the molecular mechanisms governing stigma maturation and subsequent pollen recognition are poorly characterised. …”
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Validation of the de Morton Mobility Index for measuring mobility related activities in Hungarian institutionalized older adults by Zsigmond Gyombolai, Alexandra Zimonyi-Bakó, Anna Zsófia Kubik, Brigitta Arndt, Izabella Jónásné Sztruhár, Richárdné Mayer, András Simon, Gyöngyvér Molnár, Éva Kovács

“…Maintaining institutionalized older people’s functional capacity and delaying its decline requires effective interventions. …”
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In vivo evaluation of phage therapy against Klebsiella pneumoniae using the Galleria mellonella model and molecular characterization of a novel Drulisvirus phage species by Gustavo Quispe-Villegas, Gabriela I. Alcántara-Lozano, Diego Cuicapuza, Raúl Laureano, Brenda Ayzanoa, Pablo Tsukayama, Jesús Tamariz

“…Moreover, the authors developed an in vivo preclinical model of MDR infection using Galleria mellonella larvae and successfully applied it to study the bacteriophage’s therapeutic efficacy. …”
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Modified transforaminal epidural steroid injection combined with pulsed radiofrequency: an effective treatment measure for lumbar radiculopathy by Qi Yan, Xianggu Zhong, Jiarong Li, Leyu Zhao, Junjie Niu, Dawei Song, Jinning Wang, Yun Teng, Tianyi Wu, Xiao Sun, Rui Chen, Shuangfei Wang, Jun Zou

“…At the 12-month follow-up, we noted that patients often achieved substantial pain alleviation within 6 months, and only a minor proportion of patients encountered pain recurrence by the 12th month, with no notable problems detected.ConclusionsThe modified TFESI in conjunction with PRF is a safe, cost-efficient, and successful therapy modality. …”
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Optimization of Abdominal Cavity Limitation Method for Prolongation of Peritoneal Dialysis in Experiment by Kebkalo A., Hrianyla V., Reiti A., Yatsyk I.

“…Through the “upper” drainage, 3.0 ml of 0.9% physiological solution was injected and if, after the aspiration, brilliant green was detected, there was no tightness. Also the strength of the formed scar, both functionally and histologically was assessed. …”
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Alpha 2 agonists for sedation to produce better outcomes from critical illness (A2B Trial): protocol for a multicentre phase 3 pragmatic clinical and cost-effectiveness randomised... by John Norrie, Stephen Morris, Daniel Francis McAuley, Bronagh Blackwood, Paul Dark, Matt P Wise, Anthony C Gordon, Kalliopi Kydonaki, Timothy Simon Walsh, David Hope, Christopher Weir, Gavin Perkins, Nazir I Lone, Benedict Creagh-Brown, Julia Boyd, Michael Reade, Richard Anthony Parker, Leanne M Aitken, Valerie J Page, Alasdair MacLullich, Cathrine A McKenzie, Alix Macdonald, Annabel Giddings, Lydia Emerson, Robert Glen

“…The primary outcome is time to successful extubation. Secondary ICU outcomes include delirium and coma incidence/duration, sedation quality, predefined adverse events, mortality and ICU length of stay. …”
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Construction of DF-1 Cell Line of Stably Expressing Cas9 Protein Mediated by Lentivirus and Verification of Its Activity by Xiaojiao LI, Xinyu ZHU, Xian ZOU, Xia YAN, Yanhua HE, Chenglong LUO

“…【Conclusion】The DF-1 cell line of stably expressing Cas9 protein with cleavage activity was successfully established, which could provide basic materials for the subsequent research on chicken functional genes on the DF-1 cell line of stably expressing Cas9 protein.…”
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Human umbilical cord mesenchymal stem cells improve disease characterization of Sjogren's syndrome in NOD mice through regulation of gut microbiota and Treg/Th17 cellular immunity by Yao Zou, Wei Xiao, Dongzhou Liu, Xianyao Li, Lihua Li, Lijuan Peng, Ying Xiong, Haina Gan, Xiang Ren

“…Results UC‐MSCs, UC‐MSCs‐exo, and NOD mouse splenic T cells were successfully cultured and identified. After T cells were co‐cultured with UC‐MSCs and UC‐MSCs‐exo, both IFN‐γ and IL‐6 were decreased while IL‐10, PGE2, and TGF‐β1 were increased in transcriptional and translational levels. …”
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LH Gene Cloning, Sequence Feature Analysis and Prokaryotic Expression in Conger myriaster by Yan CHEN, Bao SHI, Chenggang WANG, Wanjun BO, Kewen YAN, Meijun TAO, Xinyu ZHAO, Xiaodong MA

“…Western blot analysis revealed that histidine tagged LH protein reacted with anti-His antibody, LH protein yielded clear bands of 26.5 kDa were detected. The observed size is in accordance with the anticipated molecular weight expression, indicating successful expression of the fusion protein carrying 6×His tag. …”
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Endothelial cells nurture stem cell-like characteristics of osteosarcoma cells in a perivascular niche by CXCL12/CXCR4 pathway by CAO Ya, LIU Yang, WANG Yang

“…Flow cytometry and Western blotting were used to detect the expression of stem cell marker CD133 and stemness transcription factors SOX2 and NANOG. …”
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Lower respiratory rate during sleep in children with Angelman syndrome compared to age-matched controls by Leo Gschwind, Sebastian Camillo Holst, David Nobbs, Florian Lipsmeier, Katalin Buzasi, Ponghatai Boonsimma, Alexander Rotenberg, Vitaliy Kolodyazhniy, Jörg Felix Hipp

“…Conclusion Our study provides the first long-term observational evidence of a persistent bradypnea-like phenotype in individuals with AS, which may have significant implications for their clinical management. The successful use of the sleep mat device as a non-invasive physiological ambulatory monitoring tool demonstrates its potential as a digital health technology for detecting respiratory abnormalities in pediatric neurodevelopmental disorders. …”
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The effect of dexmedetomidine on acute kidney injury after elective major abdominal surgery : a retrospective single-center propensity score matched study by Haibei Liu, Rong Luo, Liu Qian, Yujun Zhang, Wensheng Zhang, Juan Tan, Ling Ye

“…Conclusion In this single-center retrospective propensity-matched study, we did not detect a significant overall difference in post-operative AKI rates between patients treated with or without dexmedetomidine during major abdominal surgery. …”
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Construction and validation of acetylation-related gene signatures for immune landscape analysis and prognostication risk prediction in luminal breast cancer by Mengdi Zhu, Jinna Lin, Haohan Liu, Jingru Wang, Nianqiu Liu, Yudong Li, Hongna Lai, Qianfeng Shi

“…Conclusions In this study, we successfully constructed a 6-gene acetylation-associated risk model for luminal breast cancer, providing a new direction and evidence for personalized treatment. …”
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Narva noorte väärtused ja identiteet by Andu Rämmer, Maria Žuravljova, Anne Kivimäe, Maria Anderson

“…Among them are aspirations towards a successful career, independence from parents and good looks. …”
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