Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity by Yingying Huang, Qiqi Mao, Jian He, Jing Su, Yi Peng, Wei Liang, Zixi Hu, Sufang Zhou, Xiaoling Lu, Yongxiang Zhao

“…Purified ECs (CD105+) took the function of endothelial cells, then successfully fused with DCs. …”
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Gut Microbiota Modulation by Lysozyme as a Key Regulator of Vascular Inflammatory Aging by Chenyang Zhang, Xin Zhao, Hang Zhang, Tongtong Wang, Zhenyu Zhang, Yilin Yin, Hui Wang, Xiao Tong, Yuzheng Xue, Yahong Zhou, Fenglai Yuan, Xiuwu Bian, Hong Wei, Yuan Huang, Tianhao Liu

“…Lyz1 deficiency led to vascular inflammatory aging, including damage to indicators related to oxidative stress, vascular function, and inflammation in the serum and vascular tissues of wild-type (WT) and Lyz1−/− mice. …”
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Identification of a Rare Mutation in the SRD5A2 Gene in an Iranian Family with Sex Development Disorder by Atiyeh Ataei, Peyman Eshraghi, Atiyeh Eslahi, Zeinab Khazaei, Masoome Alerasool, Sara Jamali, Faranak Ghadamyari, Majid Mojarrad

“…According to WES result and Sanger sequencing a homozygote loss of function mutation (c.16C>T; p.Gln6Ter) in SRD5A2 has been detected. …”
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Hepatic epithelioid hemangioendothelioma, a rare liver tumor: a case report and review of the literature by Chao Deng, Yulong An, Yulong An, Jinli Liu, Jinli Liu, Chao Wang, Haoxian Gou, Jinpeng Zhang, Minghui Gu, Mengmeng Li, Tao Wang, Hao Luo

“…Multiple space-occupying lesions in the liver were incidentally detected through ultrasound imaging, despite the patient being asymptomatic. …”
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Rapid and sensitive determination of bongkrekic acid with molecularly imprinted polymer-coated wooden-tip electrospray ionization mass spectrometry by Chunfei Zhong, Xinyan Li, Fan Zhang, Ning Liu, Jiewei Deng, Yunyun Yang, Tiangang Luan

“…The practical application of the method was confirmed through the successful analysis of real-world samples, including tap water, wastewater from a food processing factory, and the aqueous extracts of food samples, with BA detected at 0.296 µg/L in Tremella. …”
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Validation of a comprehensive long-read sequencing platform for broad clinical genetic diagnosis by Siddhartha Sen, Hillary P. Handler, Alec Victorsen, Zach Flaten, Aidan Ellison, Todd P. Knutson, Sarah A. Munro, Ryan J. Martinez, Charles John Billington, Jennifer J. Laffin, Sarah Bray, Pawel Mroz, Sophia Yohe, Andrew C. Nelson, Matthew Bower, Bharat Thyagarajan

“…Our findings suggest that long-read sequencing is successful in identifying diverse genomic alterations and that our pipeline functions well as the basis for a single diagnostic test for patients with suspected genetic disease.…”
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Data‐Driven Theoretical Modeling of Centrifugal Step Emulsification and Its Application in Comprehensive Multiscale Analysis by Xin Wang, Xiaolu Cai, Chao Wan, Huijuan Yuan, Shunji Li, Yiwei Zhang, Ran Zhao, Yuxi Qin, Yiwei Li, Bi‐Feng Liu, Peng Chen

“…This predictive model empowers the CASE to achieve all‐in‐one functionality, including droplet pre‐design, generation, manipulation, and on‐site detection. …”
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Impact of non-invasive oxygen reserve index versus standard SpO2 monitoring on peripheral oxygen saturation during endotracheal intubation in the intensive care unit: Protocol for... by Hugo Hille, Aurélie Le Thuaut, Pierre Asfar, Quentin Quelven, Emmanuelle Mercier, Anthony Le Meur, Jean-Pierre Quenot, Virginie Lemiale, Grégoire Muller, Martin Cour, Alexis Ferré, Asael Berge, Anaïs Curtiaud, Maxime Touron, Gaetan Plantefeve, Jean-Charles Chakarian, Jean-Damien Ricard, Gwenhael Colin, Arthur Orieux, Patrick Girardie, Mathieu Jozwiak, Manon Rouaud, Camille Juhel, Jean Reignier, Jean-Baptiste Lascarrou, CRICS-TRIGGERSEP Network

“…The primary outcome is the lowest SpO2 value from laryngoscopy to 2 minutes after successful ETI. Secondary outcomes include cognitive function on day 28. …”
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GelMA@LNP/AST Promotes eNOS-Dependent Angiogenesis Through Autophagy Activation for the Treatment of Hind Limb Ischemia by Lai L, Wu H, Peng L, Zhang Z, Wu X, Zheng S, Su Z, Chu H

“…A potential underlying mechanism could be that the autophagy induced by AST increases eNOS activity.Conclusion: GelMA@LNP/AST enables complete revascularization in female mice after hind limb ischemia, thereby achieving limb preservation and restoring motor function. Given the good therapeutic potential of the GelMA@LNP/AST in revascularization, it may become an effective strategy for successfully salvaging limbs in cases of limb ischemia.Keywords: astilbin, endothelial cells, autophagy, eNOS, angiogenesis…”
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Cloning and Analysis of the Expansin Gene BdEXP1 from Migration Plant parasitic Nematode (Bursaphelenchus doui) by Kaiyang GUO, Shifeng LIN, Rengang WANG, Li LI, Jie ZHANG, Shizhou YU, Zili WANG, Guoqin LIU

“…【Objective】An expansin gene BdEXP1 was cloned from Bursaphelenchus doui, and its expression characteristics were investigated in order to provide reference for the in-depth study of the function of BdEXP1 gene in the interactions between B. doui and hosts.…”
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Morning versus Nocturnal Heart Rate and Heart Rate Variability Responses to Intensified Training in Recreational Runners by Olli-Pekka Nuuttila, Heikki Kyröläinen, Veli-Pekka Kokkonen, Arja Uusitalo

“…Abstract Background Resting heart rate (HR) and HR variability (HRV) are widely used parameters to assess cardiac autonomic nervous system function noninvasively. While resting assessments can be performed during sleep or after awakening, it would be relevant to know how interchangeable the results of these measurements are. …”
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Effects of sinomenine on PI3K/mTOR signaling pathway and mesangial hyperplasia in rats with adriamycin-induced nephropathy by Liu Yue, Li Peng-yuan, Zhang Qing-hua, Yan Dan

“…Objective To explore the effect of sinomenine on the signal pathway of phosphatidyl-3 kinase（PI3 K）/mammalian target of rapamycin（mTOR）and mesangial hyperplasia in renal tissue of rats with adriamycin-induced nephropathy and elucidate its protective mechanism.Methods A total of 50 SD rats were selected, 10 rats were randomly injected with normal saline via caudal vein as control group, the other 40 rats were injected with adriamycin 5 mg/kg via caudal vein for 7 days to prepare nephrotic syndrome（NS）model.After successful modeling, the rats were randomly divided into the groups of model（NS）,sinomenine low-dose（25 mg/kg）,medium-dose（50 mg/kg）and high-dose（100 mg/kg）（n=10 each）.Control and NS groups received normal saline through abdominal cavity and sinomenine groups were given corresponding doses of drugs once daily for 28 days.At 24 hours after the last administration, blood and urine samples of rats in each group were collected and rats sacrificed, renal tissue samples harvested, serum creatinine（Scr）and urea nitrogen（BUN）were measured by enzyme-linked immunosorbent assay（ELISA）;24-hour urine protein was detected by Coomassie brilliant blue staining; hematoxylin-eosin staining（HE）was utilized for analyzing the pathological changes of kidney and mesangial matrix index（M/G）;transmission electron microscopy for observing the thickening of glomerular basement membrane of kidney; Western blot for detecting the expressions of p-PI3 K/PI3 K,p-mTOR/mTOR,fibronectin（FN）and type IV collagen（COL4）in renal tissue.Results As compared with control group, there were pathological damages such as the increase of glomerular mesangial matrix, the expansion of renal tubule lumen and severe thickening of glomerular basement membrane.M/G and renal function indicators Scr, BUN,24-hour urine protein and renal tissue were elevated.The protein expressions of p-PI3 K,p-mTOR,FN and COL4 rose markedly in NS group（P<0.05）.As compared with NS group, sinomenine high/medium/low dose groups alleviated pathological damage such as glomerular basement membrane thickening, M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,expressions of mTOR,FN and COL4 proteins declined markedly in sinomenine low/medium/high-dose groups（P<0.05）.As compared with low-dose sinomenine group, glomerular basement membrane thickening was the least pathologically damaged and M/G,Scr, BUN,24-hour urine protein, renal tissue p-PI3 K,p-mTOR,FN and COL4 declined markedly in sinomenine medium/high-dose group（P<0.05）.Conclusions Sinomenine may inhibit the activation of PI3 K/mTOR signaling pathway, reduce the proliferation of glomerular mesangial cells and delay the process of nephrotic syndrome.…”
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Insight into Antiviral Activity of Ag/TiO2 Nanocomposites Against Influenza H1N1 Virus and Its Antiviral Mechanism by Ma Y, Xiao X, Wang Y, Sun J, Tang P, Li J, Sun X, Xu D, Yang Z, Chen S, Liu X

“…The antiviral activities and the antiviral mechanism of ATA were investigated in detail by light microscopy, transmission electron microscopy and biological techniques such as cell cytotoxicity, 50% tissue culture infectious dose detection, western blot and reverse transcription-polymerase chain reaction.Results: These results showed the successful synthesis of ATA nanocomposite with uniform particle size and distribution. …”
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Identification and characterization of the elusive protein backbone of the immuno-dominant and species-specific Em2(G11) metacestode antigen of Echinococcus multilocularis by Philipp A. Kronenberg, Philipp A. Kronenberg, Teivi Laurimäe, Teivi Laurimäe, Michael Reinehr, Michael Reinehr, Ansgar Deibel, Sina Hasler, Peter Gehrig, Achim Weber, Peter Deplazes, Peter Deplazes, Ramon M. Eichenberger, Ramon M. Eichenberger

“…However, whether this co-eluted protein has any functional importance in the parasite-host interplay of nutrients, growth, and diagnostic significance, is not explored. …”
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Can machine-learning algorithms improve upon classical palaeoenvironmental reconstruction models? by P. Sun, P. B. Holden, H. J. B. Birks, H. J. B. Birks

“…<p>Classical palaeoenvironmental reconstruction models often incorporate biological ideas and commonly assume that the taxa comprising a fossil assemblage exhibit unimodal response functions of the environmental variable of interest. …”
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Mesangial cell-derived CircRNAs in chronic glomerulonephritis: RNA sequencing and bioinformatics analysis by Ji Hui Fan, Xiao Min Li

“…Pro-inflammatory cytokines and cell cycle stages were detected by Enzyme-linked immunosorbent assay (ELISA) and Flow Cytometry experiment, respectively. …”
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Cloning, expression of carboxylesterase BioH and improvement of its hydrolysis activity by directed evolution by XU Hongna, YU Hongwei

“…And its thermostability was detected after the enzyme was incubated at 60℃ for 40 min. …”
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Growth Toxic Effects of Metal Nanoparticles on Bacillus subtilis and Vibrio parahaemolyticus by Shiyu CHEN, Bin XIA, Lin ZHU, Shuang LI, Qiufen LI, Xuemei SUN, Wei DAI

“…Based on the ecological roles and functions of these two bacteria in marine microbial communities, this study used B. subtilis and V. parahaemolyticus isolated from Bohai Bay as test organisms. …”
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Filling knowledge gaps: A methodological approach for the rapid investigation of species with restricted populations by André Cesar Furlaneto Sampaio, Pablo Melo Hoffmann, Elivelton Marcos Gurski, Ollyver Rech Bizarro, Santiago José Elías Velazco, Marcos Bergmann Carlucci, Marília Borgo, Mayara Monteiro Ferreira, Alessandro Camargo Angelo, Christopher Thomas Blum

“…NDVI successfully detected habitat changes over time, revealing an 86.7% reduction in the focal habitat patch from 2013 to 2021, underscoring the species’ critical threat due to habitat degradation. …”
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Exploring the gut microbiota associated with peripheral nerve invasion in colorectal cancer patients and constructing predictive models by Chuanbin Chen, Qingmin Chen, Shenghai Liu, Guoxi Li, Jiawei Zhao, Jingting Huang, Tianyi Ye, Xinting Yang, Zigui Huang, Zhen Wang, Fuhai He, Mingjian Qin, Chenyan Long, Binzhe Tang, Yongqi Huang, Weizhong Tang, Jungang Liu, Xiaoliang Huang

“…Abstract Objective This study aims to explore the differences in composition, abundance, and biological functions of the gut microbiota between colorectal cancer (CRC) patients with peripheral nerve invasion (PNI) and those without peripheral nerve invasion (NPNI). …”
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