Integrated analyses of copy number variations and gene expression in lung adenocarcinoma. by Tzu-Pin Lu, Liang-Chuan Lai, Mong-Hsun Tsai, Pei-Chun Chen, Chung-Ping Hsu, Jang-Ming Lee, Chuhsing Kate Hsiao, Eric Y Chuang

“…Identification of prognostic biomarkers for lung cancer using gene expression microarrays poses a major challenge in that very few overlapping genes have been reported among different studies. …”
Get full text

From Insect to Man: Photorhabdus Sheds Light on the Emergence of Human Pathogenicity. by Geraldine Mulley, Michael L Beeton, Paul Wilkinson, Isabella Vlisidou, Nina Ockendon-Powell, Alexia Hapeshi, Nick J Tobias, Friederike I Nollmann, Helge B Bode, Jean van den Elsen, Richard H ffrench-Constant, Nicholas R Waterfield

“…Photorhabdus are highly effective insect pathogenic bacteria that exist in a mutualistic relationship with Heterorhabditid nematodes. …”
Get full text

SNPstream® UHT: Ultra-High Throughput SNP Genotyping for Pharmacogenomics and Drug Discovery by Peter A. Bell, Surendra Chaturvedi, Craig A. Gelfand, Ching Yu Huang, Michael Kochersperger, Rafael Kopla, Frank Modica, Mark Pohl, Shobha Varde, Renbin Zhao, Xiaojian Zhao, Michael T. Boyce-Jacino

“…The system employs oligonucleotide microarrays manufactured in a 384-well format on a novel glass-bottomed plate. …”
Get full text

Engineered EVs from LncEEF1G - overexpressing MSCs promote fibrotic liver regeneration by upregulating HGF release from hepatic stellate cells by Jiebin Zhang, Xiaotong Qiu, Yunguo Lei, Haitian Chen, Dongwei Wu, Tingting Wang, Xin Sui, Jiaqi Xiao, Chenhao Jiang, Huayao Zhang, Yasong Liu, Xiaoquan Liu, Yingcai Zhang, Xu Che, Ye Lin, Jia Yao, Zihao Pan, Rong Li, Jun Zheng

“…However, there is still no clinically effective treatment for promoting fibrotic liver regeneration because the underlying hepatocellular mechanism remains poorly understood. …”
Get full text

Pan-cancer landscape of ITGAV and its potential role in gastric cancer by Bin Ke, Peng Jin, Xue-Jun Wang, Ru-peng Zhang, Ning Liu, Gang Ma

“…Gene enrichment analysis was performed to explore potential functions of ITGAV in gastric cancer (GC). GC tissue microarrays and in vitro cell experiments were used to verify the prediction results in GC. …”
Get full text

Distribution of Recessive Genetic Defect Carriers in Holstein Friesian Cattle: A Polish Perspective by Marta Gozdek, Sebastian Mucha, Adam Prostek, Dariusz Kamola, Tomasz Sadkowski

“…The studies were performed using Illumina Infinium microarrays. Among both bulls and cows, the highest numbers of carriers were detected for HH5 (appropriately 6.7% and 5.4%). …”
Get full text

Transcriptomic analysis of neural stem and progenitor cells in comparison with glioblastoma stem cells by V. E. Shevchenko, N. E.  Arnotskaya, T.  I. Kushnir, A. S. Bryukhovetskiy

“…To conduct a comparative transcriptomic analysis of CD133+-NSPCs and CD133+-GSCs to study the molecular genetic differences between the phenotypes of these cells and identify potential targets for therapeutic effects on GSCs.Materials and methods. Used: highly sensitive transcriptomic analysis on high-density microarrays, cellular technologies, modern bioinformatics analysis.Results. …”
Get full text

Multi-institutional TSA-amplified Multiplexed Immunofluorescence Reproducibility Evaluation (MITRE) Study by Bernard A Fox, Chichung Wang, Kristin Roman, Carla Coltharp, Carmen Ballesteros-Merino, Shawn M Jensen, Janis M Taube, David L Rimm, Cliff Hoyt, Mei Jiang, Edwin R Parra, Ignacio Wistuba, Elizabeth L Engle, John McGuire, Bethany Remeniuk, Darren Locke

“…Serial sections from tonsil and breast carcinoma and non-small cell lung cancer (NSCLC) tissue microarrays (TMAs), TSA-Opal fluorescent detection reagents, and antibodies were distributed to the six sites equipped with a Leica Bond Rx autostainer and a Vectra Polaris multispectral imaging platform. …”
Get full text

GPX3 promotes cisplatin resistance in TNBC by manipulating ROS-TGFB1-ZEB2 by Qingyi Hu, Qianzhi Chen, Wen Yang, Anwen Ren, Jie Tan, Tao Huang

“…The correlation between the expression level of GPX3, TGFB1, and ZEB2 was analyzed using breast cancer microarrays and the TCGA database. The effect of GPX3 on platinum sensitivity in TNBC was studied using a mouse xenograft model. …”
Get full text

Phosphoribosyl transferase domain containing 1: A prognostic biomarker in testicular germ cell tumors by Peisheng Huang, Yihao Chen, Yongcheng Shi, Chuanfan Zhong, Huawei Lin, Xiaoxue Yu, Kai Chen, Zhuoya Huang, Le Zhang, Shumin Fang, Jianming Lu, Jiahong Chen

“…Immunohistochemistry on tissue microarrays validated PRTFDC1’s predictive value in clinical samples. …”
Get full text

Imputation of variants from the 1000 Genomes Project modestly improves known associations and can identify low-frequency variant-phenotype associations undetected by HapMap based i... by Andrew R Wood, John R B Perry, Toshiko Tanaka, Dena G Hernandez, Hou-Feng Zheng, David Melzer, J Raphael Gibbs, Michael A Nalls, Michael N Weedon, Tim D Spector, J Brent Richards, Stefania Bandinelli, Luigi Ferrucci, Andrew B Singleton, Timothy M Frayling

“…Our data provide important proof of principle that 1000 Genomes imputation will detect novel, low frequency-large effect associations.…”
Get full text

Targeting Mesothelin Enhances Personalized Neoantigen Vaccine Induced Antitumor Immune Response in Orthotopic Pancreatic Cancer Mouse Models by Zhixiong Cai, Zhenli Li, Wenting Zhong, Fangzhou Lin, Xiuqing Dong, Honghao Ye, Yutong Guo, Geng Chen, Xiaoling Yu, Haijun Yu, Ruijing Tang, Xiaolong Liu

“…Abstract The immunosuppressive microenvironment in pancreatic cancer, characterized by low tumor‐specific T cells and excessive fibrosis, limits the effectiveness of immunotherapy. Here, three datasets and multi‐immunofluorescence staining of tissue microarrays in pancreatic cancer indicate that mesothelin (MSLN) expression negatively correlates with cytotoxic T cells in tumor. …”
Get full text

Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1 by Yijian Deng, Liying Zeng, Huaxi Liu, Anna Zuo, Jie Zhou, Ying Yang, Yanting You, Xinghong Zhou, Baizhao Peng, Hanqi Lu, Shuai Ji, Ming Wang, Yigui Lai, Hiu Yee Kwan, Xiaomin Sun, Qi Wang, Xiaoshan Zhao

“…Finally, the inhibitory effects of silibinin on ferroptosis were reversed by knocking down FTH1 in vitro. …”
Get full text

Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1 by Guoqing Su, Jinghang Qian, Yi Wang, Yu Zhu, Yanyan Chen, Jingru Shen, Jiayuan Jiang, Yuepeng Cao, Nannan Wang, Xing Huang, Chengshuai Si, Xu Zhang, Peng Shao, Yongxia Ye, Yang Wang, Jun Bao, Liu Yang

“…Analysis of CRC tissue microarrays and TCGA-CRC patient data indicated that LINC00183 is often overexpressed in CRC, in association with advanced tumor stage and poor survival. …”
Get full text

Applicability of epigenetic age models to next-generation methylation arrays by Leonardo D. Garma, Miguel Quintela-Fandino

“…As new methylation microarrays are developed and older models discontinued, existing epigenetic clocks might become obsolete. …”
Get full text

Identification of vascular and hematopoietic genes downstream of etsrp by deep sequencing in zebrafish. by Gustavo Gomez, Jae-Hyung Lee, Matthew B Veldman, Jing Lu, Xinshu Xiao, Shuo Lin

“…Several novel genes expressed in vasculature have been identified through transcriptional profiling of zebrafish embryos overexpressing etsrp by microarrays. Here we re-examined this transcriptional profile by Illumina RNA-sequencing technology, revealing a substantially increased number of candidate genes regulated by etsrp. …”
Get full text

p53 activation by knockdown technologies. by Mara E Robu, Jon D Larson, Aidas Nasevicius, Soraya Beiraghi, Charles Brenner, Steven A Farber, Stephen C Ekker

“…Interestingly, quantitative reverse-transcriptase PCR, microarrays and whole-mount in situ hybridization assays show that MO off-targeting effects are accompanied by diagnostic transcription of an N-terminal truncated p53 isoform that uses a recently recognized internal p53 promoter. …”
Get full text

Inhibition of PDK modulates radiotherapy resistance in gastric cancer by Qi Zhang, Wanjia Qiao, Xiaoyu Liu, Jun Lu, Farooq Benish, Shuping Li, Xiaojun Liu

“…Abstract To explore the effect of sodium dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, on the progression of gastric cancer and resistance to radiotherapy, we analyzed histopathological microarrays from 60 gastric cancer and paracancerous tissues to determine PDK expression and its prognostic significance. 5-Ethynyl-2′-deoxyuridine (EdU) incorporation assay and Transwell migration assay were used to investigate the effects of PDK inhibition on gastric cancer cell proliferation and migration. …”
Get full text

The transcription factor SOX18 regulates the expression of matrix metalloproteinase 7 and guidance molecules in human endothelial cells. by Martina Hoeth, Heide Niederleithner, Renate Hofer-Warbinek, Martin Bilban, Herbert Mayer, Ulrike Resch, Christof Lemberger, Oswald Wagner, Erhard Hofer, Peter Petzelbauer, Rainer de Martin

“…Knock-down of SOX18 using RNA interference was then used to confirm the effect of the transcription factor on selected genes that included the guidance molecules ephrin B2 and semaphorin 3G. …”
Get full text

Serum LncRNAs Profiles Serve as Novel Potential Biomarkers for the Diagnosis of HBV-Positive Hepatocellular Carcinoma. by Kang Wang, Wei Xing Guo, Nan Li, Chun Fang Gao, Jie Shi, Yu Fu Tang, Feng Shen, Meng Chao Wu, Shan Rong Liu, Shu Qun Cheng

“…<h4>Methods</h4>lncRNA microarrays were utilized to measure the differential expression of lncRNAs between tumor tissues and corresponding non-tumor tissues in HBV-positive hapatocellular carcinoma. uc001ncr and AX800134 were selected as candidate lncRNAs and detected in three independent cohorts containing a total of 684 participants (healthy individuals and chronic HBV patients and HBV-positive HCC patients) who were recruited between March 2011 and December 2012. …”
Get full text