Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct by Sandra Soto-Heras, Larissa J. Volz, Nicolai Bovin, David J. Miller

“…Furthermore, the percentage of polyspermic zygotes was reduced in the suLeX-coverslip method (17.7 vs. 41.3%, p < 0.05). …”
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The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules. by Adam Ahmad, Yoel Bogoch, Gal Shvaizer, Noga Guler, Karine Levy, Yaniv M Elkouby

“…Mechanistically, we provide evidence to conclude that zygotic Asz1 is not required for primordial germ cell specification or migration to the gonad, but is essential during post-embryonic gonad development, likely by suppressing the expression of germline transposons. …”
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Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid. by Verónica M Negrón-Pérez, Abdullah Al Naib, Abigail L Zezeski, Victoria L McCracken-Harlow, George A Perry, Alan D Ealy, Michelle L Rhoads

“…In general, FF supplementation at 50% did not affect the zygotes' developmental potential (neither increased nor decreased). …”
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Homeobox genes encoding WOX transcription factors in the flowering parasitic plant Monotropa hypopitys by A. V. Shchennikova, O. A. Shulga, E. Z. Kochieva, A. V. Beletsky, M. A. Filyushin, N. V. Ravin, K. G. Skryabin

“…The assumption is that the MhyWUS1 and MhyWUS2 genes maintain the stem cell population in the inflorescence and flower meristems, MhyWOX13 has a role in the control of root stem cell niche, seed pod formation, flowering initiation, and basic cellular processes, MhyWOX4 functions in the control of cambium stem cells, and MhyWOX2 participates in the differentiation of egg cells and zygotes.…”
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Biotechnological methods as a tool for efficient sugar beet breeding by T. P. Zhuzhzhalova, E. O. Kolesnikova, E. N. Vasilchenko, N. N. Cherkasova

“…The results of studies on the culturing of mature sugar beet zygotic embryos based on in vitro selective systems have made it possible to improve the adaptive potential of plants and to provide complex resistance to environmental stress factors. …”
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Embryo Density and Its Neutrality in Day-3 Embryo Development: A Retrospective Cohort Study by Cheng Shi, Tie Cheng Sun, Shi Hui Meng, Ping Wang, Rong Liang, Sheng Nan Duan, Hong Jing Han, Ya Nan Liu, Huan Shen, Xi Chen

“…In a 30 μl microdrop, the culturing embryos with embryo densities of 15, 10, and 30 μl/embryo (from zygotes to day 3) had similar developmental outcomes. …”
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GWAS identifies a polyembryony locus in mango: development of KASP and PACE markers for marker-assisted breeding by Gul Shad Ali, Shamseldeen Eltaher, Shamseldeen Eltaher, Jin Li, Barbie Freeman, Sukhwinder Singh

“…Many mango cultivars exhibit apomictic polyembryony, where one embryo develops from zygotic tissues and the rest from nucellar tissues, resulting in seedlings that are genetically identical to the mother tree. …”
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Mir-449b improves the epigenetic status of buffalo transgenic embryos produced by handmade cloning by Gaurav Tripathi

“…After incubation, transfected zygotes were chemically activated and subjected to in vitro culture in RVCL (Cook®) medium for 8 days at 38.5°C in 5% CO2 in air, 90- 95% relative humidity condition. …”
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Factors accounting for limited sexual reproduction in a long-lived unisexual plant species by Irene Bisang, Flavien Collart, Flavien Collart, Alain Vanderpoorten, Lars Hedenäs

“…We further infer that environmental conditions at the pre-zygotic stage have lower than expected effects on the overall distribution of this moss. …”
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One carbon metabolism supplementation in maturation medium but not embryo culture medium improves the yield of blastocysts from bovine oocytes by Arefeh Golestanfar, Amir Niasari Naslaji, Farnoosh Jafarpour, Nima Sadeghi Borujen, Shiva Rouhollahi Varnosfaderani, Yves Menezo, Maurizio Dattilo, Mohammad Hossein Nasr-Esfahani

“…After in vitro fertilization, the putative zygotes from each treatment (IVM or Sup-IVM) were cultured in the media either without (IVM/IVC or Sup-IVM/IVC) or with (IVM/Sup-IVC or Sup-IVM/Sup-IVC) OCM supplementation. …”
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Cloned buffalo bulls and their somatic cell donor bulls exhibit similar in-vitro embryo production efficienc by Tanya Gupta, Gaurav Tripathi, Sonal Gupta, Naresh L. Selokar, Manoj Kumar Singh

“…After 24 h, mature oocytes were co-incubated with processed semen in the fertilization Bracket and Oliphant (BO) medium for 18 h. The presumptive zygotes were cultured in Research Vitro Cleavage medium (RVCL; Cook, Australia) for up to 8 days. …”
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Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“…After maturing with epinephrine, oocytes were fertilized for 6 hours with post-thawed semen from a single bull. Presumptive zygotes were cultured in commercial media (IVF Bioscience, Falmouth, UK), and cleavage was observed 48 hours after fertilization. …”
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Ovarian stimulation alternatives for in vitro production of embryos in water buffaloes by Alexis Bandeo, José Luis Konrad, Natalia Vallejos, Pablo Ponce, Marina J. Sansinena, Gustavo Crudeli, Pablo Maldonado-Vargas

“…No significant difference was found in the number of zygotes (CIV) between treatments (p<0.05), and cleavage was higher in TRT3 (p<0.05). …”
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