Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination by Michal Safran, William Y. Kim, Andrew L. Kung, James W. Horner, Ron A. DePinho, William G. Kaelin

“…ROSA26 L-S-L-Luc /+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. …”
Get full text

Gestational exposure to arsenic reduces female offspring fertility by impairing the repair of DNA double-strand breaks and synapsis formation in oocytes by Cong Ma, Kaiqin Hu, Xueke Zhang, Yaru Yu, Zuying Xu, Tian Ma, Hongzhen Ruan, Yingying Zhang, Jiajia Wang, Xinyu Yue, Chunmei Liang, Huifen Xiang, Rui Guo, Yunxia Cao, Zhiming Ding

“…By assessing the in vitro developmental capacity of oocytes and zygotes, we confirmed that the reduced fertility was due not to impaired oocyte quality but rather to a reduction in oocyte quantity. …”
Get full text

Development of Transgenic Papaya through Agrobacterium-Mediated Transformation by Md. Abul Kalam Azad, Md. Golam Rabbani, Latifah Amin, Nik Marzuki Sidik

“…Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene. …”
Get full text

Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos by Vibuntita Chankitisakul, Theerawat Tharasanit, Kriengsak Tasripoo, Mongkol Techakumphu

“…At 18 h after IVF, most presumptive zygotes had reached the pronuclear stage. The sperm aster was concurrently enlarged to assist the migration and apposition of pronuclei. …”
Get full text

Induction of somatic embryogenesis and cryopreservation of Abies pinsapo Boiss. by Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas, Carolina Sánchez-Romero

“…Somatic embryogenesis was induced from mature zygotic embryos of A. pinsapo cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L-1 sucrose and 5 mg L-1 6-benzylaminopurine (BA). …”
Get full text

Genome editing using CRISPR/ Cas9 system: a practical guide by A. G. Menzorov, V. A. Lukyanchikova, A. N. Korablev, I. A. Serova, V. S. Fishman

“…Finally, we report protocols of genome editing for modification of embryonic stem cells and zygotes.…”
Get full text

The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. by David G Ransom, Nathan Bahary, Knut Niss, David Traver, Caroline Burns, Nikolaus S Trede, Noelle Paffett-Lugassy, Walter J Saganic, C Anthoney Lim, Candace Hersey, Yi Zhou, Bruce A Barut, Shuo Lin, Paul D Kingsley, James Palis, Stuart H Orkin, Leonard I Zon

“…Surprisingly, a high level of zygotic tif1gamma mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. …”
Get full text

Phylogeography and hybridization of corvid birds in the Palearctic Region by A. P. Kryukov

“…In their young contact zone, some hybridization occurs with small introgression limited by certain post-zygotic isolation.…”
Get full text

Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos by I. O. Bogolyubova, Z. K. Sailau, D. S. Bogolyubov

“…Immunocytochemical data suggest that the molecular composition of this ‘ring heterochromatin’ in mouse embryos changes during the realization of embryonic genome activation events, as well as during artificial suppression of transcription. In zygotes, some factors of mRNA biogenesis including splicing factor SC35 (SRSF2) and basal transcription factor TFIID are detectable in the ring chromatin. …”
Get full text

Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct by Sandra Soto-Heras, Larissa J. Volz, Nicolai Bovin, David J. Miller

“…Furthermore, the percentage of polyspermic zygotes was reduced in the suLeX-coverslip method (17.7 vs. 41.3%, p < 0.05). …”
Get full text

The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules. by Adam Ahmad, Yoel Bogoch, Gal Shvaizer, Noga Guler, Karine Levy, Yaniv M Elkouby

“…Mechanistically, we provide evidence to conclude that zygotic Asz1 is not required for primordial germ cell specification or migration to the gonad, but is essential during post-embryonic gonad development, likely by suppressing the expression of germline transposons. …”
Get full text

Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid. by Verónica M Negrón-Pérez, Abdullah Al Naib, Abigail L Zezeski, Victoria L McCracken-Harlow, George A Perry, Alan D Ealy, Michelle L Rhoads

“…In general, FF supplementation at 50% did not affect the zygotes' developmental potential (neither increased nor decreased). …”
Get full text

Homeobox genes encoding WOX transcription factors in the flowering parasitic plant Monotropa hypopitys by A. V. Shchennikova, O. A. Shulga, E. Z. Kochieva, A. V. Beletsky, M. A. Filyushin, N. V. Ravin, K. G. Skryabin

“…The assumption is that the MhyWUS1 and MhyWUS2 genes maintain the stem cell population in the inflorescence and flower meristems, MhyWOX13 has a role in the control of root stem cell niche, seed pod formation, flowering initiation, and basic cellular processes, MhyWOX4 functions in the control of cambium stem cells, and MhyWOX2 participates in the differentiation of egg cells and zygotes.…”
Get full text

Biotechnological methods as a tool for efficient sugar beet breeding by T. P. Zhuzhzhalova, E. O. Kolesnikova, E. N. Vasilchenko, N. N. Cherkasova

“…The results of studies on the culturing of mature sugar beet zygotic embryos based on in vitro selective systems have made it possible to improve the adaptive potential of plants and to provide complex resistance to environmental stress factors. …”
Get full text

Embryo Density and Its Neutrality in Day-3 Embryo Development: A Retrospective Cohort Study by Cheng Shi, Tie Cheng Sun, Shi Hui Meng, Ping Wang, Rong Liang, Sheng Nan Duan, Hong Jing Han, Ya Nan Liu, Huan Shen, Xi Chen

“…In a 30 μl microdrop, the culturing embryos with embryo densities of 15, 10, and 30 μl/embryo (from zygotes to day 3) had similar developmental outcomes. …”
Get full text

GWAS identifies a polyembryony locus in mango: development of KASP and PACE markers for marker-assisted breeding by Gul Shad Ali, Shamseldeen Eltaher, Shamseldeen Eltaher, Jin Li, Barbie Freeman, Sukhwinder Singh

“…Many mango cultivars exhibit apomictic polyembryony, where one embryo develops from zygotic tissues and the rest from nucellar tissues, resulting in seedlings that are genetically identical to the mother tree. …”
Get full text

Mir-449b improves the epigenetic status of buffalo transgenic embryos produced by handmade cloning by Gaurav Tripathi

“…After incubation, transfected zygotes were chemically activated and subjected to in vitro culture in RVCL (Cook®) medium for 8 days at 38.5°C in 5% CO2 in air, 90- 95% relative humidity condition. …”
Get full text

Factors accounting for limited sexual reproduction in a long-lived unisexual plant species by Irene Bisang, Flavien Collart, Flavien Collart, Alain Vanderpoorten, Lars Hedenäs

“…We further infer that environmental conditions at the pre-zygotic stage have lower than expected effects on the overall distribution of this moss. …”
Get full text

One carbon metabolism supplementation in maturation medium but not embryo culture medium improves the yield of blastocysts from bovine oocytes by Arefeh Golestanfar, Amir Niasari Naslaji, Farnoosh Jafarpour, Nima Sadeghi Borujen, Shiva Rouhollahi Varnosfaderani, Yves Menezo, Maurizio Dattilo, Mohammad Hossein Nasr-Esfahani

“…After in vitro fertilization, the putative zygotes from each treatment (IVM or Sup-IVM) were cultured in the media either without (IVM/IVC or Sup-IVM/IVC) or with (IVM/Sup-IVC or Sup-IVM/Sup-IVC) OCM supplementation. …”
Get full text

Cloned buffalo bulls and their somatic cell donor bulls exhibit similar in-vitro embryo production efficienc by Tanya Gupta, Gaurav Tripathi, Sonal Gupta, Naresh L. Selokar, Manoj Kumar Singh

“…After 24 h, mature oocytes were co-incubated with processed semen in the fertilization Bracket and Oliphant (BO) medium for 18 h. The presumptive zygotes were cultured in Research Vitro Cleavage medium (RVCL; Cook, Australia) for up to 8 days. …”
Get full text