Introduction of the single-lumen oocyte retrieval needle in an assisted reproductive technology center: Propensity score matching analysis of grouping by Tian-Jeau Huang, Hsiao-Chin Huang, Horng-Der Tsai, Chew-Teng Kor, Chueh-Ko Yang, Hsin-Hung Wu

“…Outcomes included oocytes retrieved, matured oocytes (MII), normal zygotes (2-PN), viable embryos, procedure duration and pregnancy rate. …”
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Genomic Copy Number Variation Affecting Genes Involved in the Cell Cycle Pathway: Implications for Somatic Mosaicism by Ivan Y. Iourov, Svetlana G. Vorsanova, Maria A. Zelenova, Sergei A. Korostelev, Yuri B. Yurov

“…Accordingly, we speculate that further studies of CNV burden across the genes implicated in related pathways might clarify whether zygotic genomic variation generates somatic mosaicism in health and disease.…”
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Dynamic reprogramming of DNA methylation at an epigenetically sensitive allele in mice. by Marnie E Blewitt, Nicola K Vickaryous, Andras Paldi, Haruhiko Koseki, Emma Whitelaw

“…We have analysed the DNA methylation in mature gametes, zygotes, and blastocysts and found that the paternally and maternally inherited alleles are treated differently. …”
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NaP-TRAP reveals the regulatory grammar in 5’UTR-mediated translation regulation during zebrafish development by Ethan C. Strayer, Srikar Krishna, Haejeong Lee, Charles Vejnar, Nils Neuenkirchen, Amit Gupta, Jean-Denis Beaudoin, Antonio J. Giraldez

“…We also observe a translational switch during the maternal-to-zygotic transition, where C-rich motifs shift from repressors to prominent activators of translation. …”
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The effect of l-ergothioneine and fetuin on blastocyst development rates by Sakine Ülküm Çizmeci, Dursun Ali Dinç, Mustafa Numan Bucak, Ömer Faruk Yeşilkaya, Vahit Ağır, Maide Gölbaşı, Ayşe Sarı, Hasan Ali Çay

“…After the fertilization step, zygotes were transferred in to culture (IVC) drops with antioxidant (Grup 1: L-ergothionine (L-Erg) 0.05 mM (n:100), Grup 2: fetuin 1 mg/ml (n:100), Grup 3: control (n:120)). …”
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PPARGC1A regulates transcriptional control of mitochondrial biogenesis in early bovine embryos by Muhammad Idrees, Muhammad Idrees, Zaheer Haider, Chalani Dilshani Perera, Safeer Ullah, Seo-Hyeon Lee, Seung Eun Lee, Sung-Sik Kang, Sung Woo Kim, Il-Keun Kong, Il-Keun Kong, Il-Keun Kong

“…This study investigated the role of PGC-1α from a single-cell zygotic stage to day-8 bovine blastocyst and the effect of PGC-1a knockdown (KD) on embryo mitochondria and development. …”
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Application of genome tagging technology in elucidating the function of sperm-specific protein 411 (Ssp411) by Xue-Hai Zhou, Min-Min Hua, Jia-Nan Tang, Bang-Guo Wu, Xue-Mei Wang, Chang-Gen Shi, Yang Yang, Jun Wu, Bin Wu, Bao-Li Zhang, Yi-Si Sun, Tian-Cheng Zhang, Hui-Juan Shi

“…Ssp411 is not detectable in metaphase II (MII) oocytes, zygotes, or 2-cell stage embryos, highlighting its intricate role in early embryonic development. …”
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Parentage Verification and Segregation Distortion Patterns of Microsatellite Markers in Olive Flounder (<i>Paralichthys olivaceus</i>) Full-Sib Families by Songhyun Gwon, Eunjeong Kim, Wonse Lee, Jisung Han, Yoonkwon Nam

“…Segregation distortion was observed at certain loci, deviating from Mendelian inheritance, likely due to meiotic drive and post-zygotic selection. These findings underscore the need for empirical validation of microsatellite loci for stable inheritance and reliable parentage in diverse breeding programs, especially with genetically similar spawners.…”
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Sperm-borne small non-coding RNAs: potential functions and mechanisms as epigenetic carriers by Muhammad Naveed, Zhaokang Shen, Jianqiang Bao

“…Additionally, the review delves into the potential mechanisms by which sperm-delivered sncRNAs may acquire and transmit paternally acquired traits to offspring, modulating zygotic gene expression and influencing early embryonic development.…”
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Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination by Michal Safran, William Y. Kim, Andrew L. Kung, James W. Horner, Ron A. DePinho, William G. Kaelin

“…ROSA26 L-S-L-Luc /+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. …”
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Gestational exposure to arsenic reduces female offspring fertility by impairing the repair of DNA double-strand breaks and synapsis formation in oocytes by Cong Ma, Kaiqin Hu, Xueke Zhang, Yaru Yu, Zuying Xu, Tian Ma, Hongzhen Ruan, Yingying Zhang, Jiajia Wang, Xinyu Yue, Chunmei Liang, Huifen Xiang, Rui Guo, Yunxia Cao, Zhiming Ding

“…By assessing the in vitro developmental capacity of oocytes and zygotes, we confirmed that the reduced fertility was due not to impaired oocyte quality but rather to a reduction in oocyte quantity. …”
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Development of Transgenic Papaya through Agrobacterium-Mediated Transformation by Md. Abul Kalam Azad, Md. Golam Rabbani, Latifah Amin, Nik Marzuki Sidik

“…Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene. …”
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Uncovering somatic mosaic variants of PIK3CA-related overgrowth disorders – three cases with different clinical presentations by M. Tooming, M. Tooming, P. Mertsina, T. Kahre, T. Kahre, R. Teek, I. Vainumäe, I. Vainumäe, S. Lilles, S. Lilles, M. H. Wojcik, M. H. Wojcik, M. H. Wojcik, P. Ilves, P. Ilves, K. Õunap, K. Õunap

“…PRD typically manifest during the post-zygotic phase, leading to a broad spectrum of overgrowth and vascular malformations affecting various body regions.MethodsConventional diagnostic methods struggle to detect and confirm pathogenic PIK3CA gene variants due to the mosaic nature of these disorders and the limited accessibility of affected tissues. …”
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Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos by Vibuntita Chankitisakul, Theerawat Tharasanit, Kriengsak Tasripoo, Mongkol Techakumphu

“…At 18 h after IVF, most presumptive zygotes had reached the pronuclear stage. The sperm aster was concurrently enlarged to assist the migration and apposition of pronuclei. …”
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MARTRE family proteins negatively regulate CCR4-NOT activity to protect poly(A) tail length and promote translation of maternal mRNA by Jing Yang, Jiachen Bu, Bowen Liu, Yusheng Liu, Zhuqiang Zhang, Ziyi Li, Falong Lu, Bing Zhu, Yingfeng Li

“…Deletion of the Martre gene locus results in shortened poly(A) tails and decreased translation efficiency of actively translated mRNAs in mouse zygotes, but does not affect maternal mRNA decay. …”
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Induction of somatic embryogenesis and cryopreservation of Abies pinsapo Boiss. by Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas, Carolina Sánchez-Romero

“…Somatic embryogenesis was induced from mature zygotic embryos of A. pinsapo cultured on solid MS medium with macroelements at half-strength and supplemented with 20 g L-1 sucrose and 5 mg L-1 6-benzylaminopurine (BA). …”
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Genome editing using CRISPR/ Cas9 system: a practical guide by A. G. Menzorov, V. A. Lukyanchikova, A. N. Korablev, I. A. Serova, V. S. Fishman

“…Finally, we report protocols of genome editing for modification of embryonic stem cells and zygotes.…”
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The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. by David G Ransom, Nathan Bahary, Knut Niss, David Traver, Caroline Burns, Nikolaus S Trede, Noelle Paffett-Lugassy, Walter J Saganic, C Anthoney Lim, Candace Hersey, Yi Zhou, Bruce A Barut, Shuo Lin, Paul D Kingsley, James Palis, Stuart H Orkin, Leonard I Zon

“…Surprisingly, a high level of zygotic tif1gamma mRNA expression delineates ventral mesoderm during hematopoietic stem cell and progenitor formation prior to gata1 expression. …”
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Phylogeography and hybridization of corvid birds in the Palearctic Region by A. P. Kryukov

“…In their young contact zone, some hybridization occurs with small introgression limited by certain post-zygotic isolation.…”
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Peculiarities of the molecular composition of heterochromatin associated with pronucleoli in mouse embryos by I. O. Bogolyubova, Z. K. Sailau, D. S. Bogolyubov

“…Immunocytochemical data suggest that the molecular composition of this ‘ring heterochromatin’ in mouse embryos changes during the realization of embryonic genome activation events, as well as during artificial suppression of transcription. In zygotes, some factors of mRNA biogenesis including splicing factor SC35 (SRSF2) and basal transcription factor TFIID are detectable in the ring chromatin. …”
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