Knockdown of Zebrafish Blood Vessel Epicardial Substance Results in Incomplete Retinal Lamination by Yu-Ching Wu, Ruei-Feng Chen, Chia-Yang Liu, Fung Rong Hu, Chang-Jen Huang, I-Jong Wang

“…In the current study, we generated a transgenic zebrafish Bves (zbves) promoter-EGFP zebrafish line to investigate the expression pattern of Bves in the retina and to study the role of zbves in retinal lamination. …”
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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

“…Summary: Characterization of zebrafish mutants is routinely time consuming as morphological phenotypes can be subtle or variable, thus hampering robust quantification. …”
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Dose-dependent effects of gamma radiation on the early zebrafish development and gene expression. by Selma Hurem, Leonardo Martín Martín, Dag Anders Brede, Eystein Skjerve, Rasoul Nourizadeh-Lillabadi, Ole Christian Lind, Terje Christensen, Vidar Berg, Hans-Christian Teien, Brit Salbu, Deborah Helen Oughton, Peter Aleström, Jan Ludvig Lyche

“…The present work focuses on radiation-induced biological effects using the zebrafish (Danio rerio) vertebrate model. Changes in developmental traits and gene expression in zebrafish were assessed after continuous external gamma irradiation (0.4, 3.9, 15 and 38 mGy/h) with corresponding controls, starting at 2.5 hours post fertilization (hpf) and lasting through embryogenesis and the early larval stage. …”
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Evaluation of the Hair Cell Regeneration in Zebrafish Larvae by Measuring and Quantifying the Startle Responses by Changquan Wang, Zhenmin Zhong, Peng Sun, Hanbing Zhong, Hongzhe Li, Fangyi Chen

“…The zebrafish has become an established model organism for the study of hearing and balance systems in the past two decades. …”
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Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs by Florian P. Weissenboeck, Melissa Pieper, Helena Schepers, Sophie Hötte, Nils Klöcker, Sabine Hüwel, Andreas van Impel, Stefan Schulte-Merker, Andrea Rentmeister

“…We demonstrate that translation of FlashCap-eGFP-mRNA can be triggered in zebrafish embryos with spatiotemporal control. The injected FlashCap-mRNA is stable for hours and remains muted. …”
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Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses by Karim Abu Nahia, Cecilia Lanny Winata

“…Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. …”
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Spatially ordered recruitment of fast muscles in accordance with movement strengths in larval zebrafish by Sayaka Shimizu, Taisei Katayama, Nozomi Nishiumi, Masashi Tanimoto, Yukiko Kimura, Shin-ichi Higashijima

Subjects: “…Zebrafish…”
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Trehalose Ameliorates Zebrafish Emotional and Social Deficits Caused by CLN8 Dysfunction by Rosario Licitra, Stefania Della Vecchia, Lorenzo Santucci, Rachele Vivarelli, Sara Bernardi, Filippo M. Santorelli, Maria Marchese

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THE EFFECT OF DIFFERENT LEVELS OF VITAMIN E ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (Danio rerio) by N.B.P Utomo, M. Zairin Junior, T.L. Yusuf, I. Mokoginta, M. Bintang

“… This experiment was conducted to determine the dietary Vitamin E requirement for reproduction of broodstock zebrafish Danio rerio.  Four isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, C, and D with different levels of Vitamin E were fed to zebrafish broodstock.  …”
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Background predation risk induces anxiety-like behaviour and predator neophobia in zebrafish by Himal Thapa, Arash Salahinejad, Adam L. Crane, Ahmad Ghobeishavi, Maud C. O. Ferrari

“…Although spatial and object neophobia in zebrafish have received previous attention, little is known about the role of background risk in inducing neophobia in zebrafish. …”
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L-theanine modulates tissue cortisol levels in zebrafish after single stress by Vinícius Luís da Silva, Mariana Spanamberg Mayer, Andriele dos Reis Severo, Andressa Leal Zambra, Joanna Moraes De Almeida, Laura Moura Sestari, Mariana Migliorini Parisi, Ana Cristina Vendrametto Varrone Giacomini, Murilo Sander De Abreu, Josiane Woutheres Bortolotto, Gabriela Bonfanti-Azzolin

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Marigold: a machine learning-based web app for zebrafish pose tracking by Gregory Teicher, R. Madison Riffe, Wayne Barnaby, Gabrielle Martin, Benjamin E. Clayton, Josef G. Trapani, Gerald B. Downes

Subjects: “…Zebrafish…”
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Dimethomorph induces heart and vascular developmental defects by disrupting thyroid hormone in zebrafish embryos by You Wei, Yunlong Meng, Kun Jia, Weijian Lu, Yushan Huang, Huiqiang Lu

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Knockout of <i>dhx38</i> Causes Inner Ear Developmental Defects in Zebrafish by Mengmeng Ren, Xiang Chen, Liyan Dai, Jiayi Tu, Hualei Hu, Xiaohan Sun, Jiong Luo, Pei Li, Yiyang Fu, Yuejie Zhu, Weiqiang Sun, Zhaohui Tang, Mugen Liu, Xiang Ren, Qunwei Lu

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Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack by Atanu Pramanik, Shubham Nema, Yogesh Bhargava, Anamika Bhargava

“…Here, we present a protocol using ZebraTrack, an open-source ImageJ-based approach for distraction-free swimming video acquisition and automated tracking of adult zebrafish. We detail steps for mathematical calculations on 2D coordinates to extract various behavioral endpoints for anxiety-behavioral evaluation.For complete details on the use and execution of this protocol, please refer to Nema et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Neuroblastoma-derived hypoxic extracellular vesicles promote metastatic dissemination in a zebrafish model. by Anna Fietta, Pina Fusco, Giuseppe Germano, Sara Micheli, Marco Sorgato, Giovanni Lucchetta, Elisa Cimetta

“…The zebrafish (Danio rerio) is a valuable model organism for studying human biology due to its easy genetic manipulation and small size. …”
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Integrated multi-omics analysis reveals the underlying molecular mechanism for the neurotoxicity of triclosan in zebrafish by Shasha Zhao, Yuhang Ling, Baohua Zhang, Danting Wang, Limei Sun

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Chronic effects of palm oil mill effluent on the hepatosomatic index of zebrafish (Danio rerio) by Zulfahmi Ilham, Setia Batubara Agung, Ali Rizwan, Wulandari Nasution Ayu, Puspita Bela, Nafis Badratun

“…This study evaluated the effects of chronic POME exposure on the HSI of zebrafish (Danio rerio) to understand their physiological adaptations to POME contamination. …”
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Cognitive Bias in Adult Zebrafish (<i>Danio rerio</i>): A Systematic Review by Valentina Gazzano, Asahi Ogi, Francesca Cecchi, Maria Claudia Curadi, Maria Marchese, Angelo Gazzano

Subjects: “…zebrafish…”
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Lactoferrin Decreases the Intestinal Inflammation Triggered by a Soybean Meal-Based Diet in Zebrafish by Pilar E. Ulloa, Camila J. Solís, Javiera F. De la Paz, Trevor G. S. Alaurent, Mario Caruffo, Adrián J. Hernández, Patricio Dantagnan, Carmen G. Feijóo

“…These results demonstrate that LF displays a dual effect in zebrafish, acting as an intestinal anti-inflammatory agent and improving performance against bacterial infection.…”
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