Nuclear farnesoid X receptor protects against bone loss by driving osteoblast differentiation through stabilizing RUNX2 by Qi Dong, Haoyuan Fu, Wenxiao Li, Xinyu Ji, Yingchao Yin, Yiran Zhang, Yanbo Zhu, Guoqiang Li, Huiyang Jia, Heng Zhang, Haofei Wang, Jinglue Hu, Ganggang Wang, Zhihao Wu, Yingze Zhang, Sujuan Xu, Zhiyong Hou

“…Mechanistically, FXR could stabilize RUNX2 by inhibiting Thoc6-mediated ubiquitination, thereby promoting osteogenic activity in BMSCs. …”
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USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation by Na Shao, Lei Xi, Yangfan Lv, Muhammad Idris, Lin Zhang, Ya Cao, Jingyi Xiang, Xi Xu, Belinda X. Ong, Qiongyi Zhang, Xu Peng, Xiaoyan Yue, Feng Xu, Chungang Liu

“…This study reveals a ubiquitination-dependent regulation of YTHDF1, proposing USP5 inhibition alongside PD-(L)1 blockade as a promising cancer treatment strategy.…”
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The protective effects of peptides from Chinese baijiu on AAPH-induced oxidative stress in HepG2 cells via Nrf2-mediated signaling pathway by Jiaying Huo, Yuezhang Ming, Huifeng Li, Anjun Li, Jiwen Zhao, Mingquan Huang, Weizheng Sun, Jihong Wu, Jinglin Zhang

“…Further experiments proved that these peptides exerted antioxidant effects via Nrf2/ARE-mediated signaling pathway by promoting Nrf2 nuclear translocation, inhibiting ubiquitination, and enhancing transcription capacity of Nrf2 in HepG2 cells. …”
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MRTX1133 attenuates KRASG12D mutated-colorectal cancer progression through activating ferroptosis activity via METTL14/LINC02159/FOXC2 axis by Junwei Zou, Xiuhua Shi, Zhaoying Wu, Siyuan Zuo, Xiaolei Tang, Hailang Zhou, Yong Huang

“…Additionally, LINC02159 stabilised FOXC2 expression through de-ubiquitination. Rescue experiments further clarified that the METTL14/LINC02159/FOXC2 signalling axis is crucial for the inhibitory effects of MRTX1133 in KRASG12D-mutated CRC. …”
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USP4-mediated CENPF deubiquitylation regulated tumor metastasis in colorectal cancer by Zhongdong Xie, Hanbin Lin, Yuecheng Wu, Yanan Yu, Xintong Liu, Yating Zheng, Xiaojie Wang, Jiashu Wu, Meifang Xu, Yuting Han, Qiongying Zhang, Yu Deng, Lin Lin, Yan Linzhu, Li Qingyun, Xinjian Lin, Ying Huang, Pan Chi

“…Intriguingly, we found CENPF undergoes degradation in CRC via the ubiquitination-proteasome pathway. Mechanistically, we observed that USP4 interacted with and stabilized CENPF via deubiquitination. …”
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IL-17 triggers PD-L1 gene transcription in NSCLC cells via TRIM31-dependent MEF2C K63-linked polyubiquitination by Shuai Ying, Ningxia Wu, Yuting Ruan, Wen Ge, Pei Ma, Tongpeng Xu, Yongqian Shu, Yingwei Wang, Wen Qiu, Chenhui Zhao

“…Co-IP/IB was used to examine the interaction between MEF2C and PD-L1, including MEF2C ubiquitination. IHC staining was carried out to analyse the expression of IL-17RA, MEF2C, TRIM31, and PD-L1 in NSCLC tissue arrays. …”
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The Legionella pneumophila effector PieF modulates mRNA stability through association with eukaryotic CCR4−NOT by Harley O'Connor Mount, Malene L. Urbanus, Francesco Zangari, Anne-Claude Gingras, Alexander W. Ensminger

“…With a host range that spans billions of years of evolution (from protists to humans), the interaction between L. pneumophila and its hosts frequently involves conserved eukaryotic pathways (protein translation, ubiquitination, membrane trafficking, autophagy, and the cytoskeleton). …”
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PER3 suppresses tumor metastasis of oral squamous cell carcinoma by promoting HIF-1α degradation by Yaoxu Li, Bing Li, Kai Yang, Lihua Zhu, Hong Tang, Yinpei Huang, Jinhai Deng, Jun Duan

“…Mechanistically, PER3 bound to HIF-1α via the Per-ARNT-Sim 1 domain and promoted its ubiquitination degradation. Hypermethylation at CpG site cg12258811 of PER3 promoter inhibited PER3 expression and prognosis of OSCC. …”
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CDC20‐Mediated Selective Autophagy Degradation of PBRM1 Affects Immunotherapy for Renal Cell Carcinoma by Yizeng Fan, Weichao Dan, Taotao Que, Yi Wei, Bo Liu, Zixi Wang, Yulin Zhang, Yuzhao Wang, Tianjie Liu, Yanxin Zhuang, Mengxing Li, Chendong Guo, Jin Zeng, Bohan Ma, Lei Li

“…Meanwhile, CDC20 catalyzes K27 ubiquitination of PBRM1 and promotes its degradation via p62‐mediated selective autophagy. …”
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Repurposing of phosphodiesterase-5 inhibitor sildenafil as a therapeutic agent to prevent gastric cancer growth through suppressing c-MYC stability for IL-6 transcription by Zhenzhan Zhang, Wuqing Huang, Donghua Huang, Zhou Xu, Qingfeng Xie, Xin Tan, Wenjun He, Weihao Yang, Guoxin Li, Jianguang Ji, Hao Liu

“…Mechanistically, sildenafil restrained GC growth by directly activating PKG through PDE5 inhibition for regulating c-MYC expression via its phosphorylation and ubiquitination degradation, thereby suppressing c-MYC stability for IL-6 transcription within the downstream IL-6/JAK/STAT3 signalling pathway. …”
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EPIGENETICS TODAY AND TOMORROW by B. F. Vanyushin

“…The most known epigenetic mechanisms (signals) are enzymatic DNA methylation, the histone code (various enzymatic histone modifications including acetylation, methylation, phosphorylation, ubiquitination and others), and gene silencing mediated by small RNAs (miRNA, siRNA). …”
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Tp53 determines the spatial dynamics of M1/M2 tumor-associated macrophages and M1-driven tumoricidal effects by Yi-Jing Hsiao, Min-Shu Hsieh, Gee-Chen Chang, Yin-Chen Hsu, Chia-Yu Wang, Yan-Ming Chen, Yi-Ling Chen, Pan-Chyr Yang, Sung-Liang Yu

“…This activation facilitates p53-STAT1 interactions, reduces the interaction between p53 and MDM2, and subsequently decreases p53 ubiquitination. M1 CM inhibited tumorigenesis, and silencing p53 reduced the anti-tumor efficacy of polyinosinic:polycytidylic acid (poly I:C) in vivo. …”
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Chronic NaAsO2 exposure promotes migration and invasion of prostate cancer cells by Akt/GSK-3β/β-catenin/TCF4 axis-mediated epithelial-mesenchymal transition by Zhi-Hui Zhang, Hai-Xin Yan, Ming-Dong Liu, Feng-Wen Niu, Kai Yao, Shi-Yao Feng, Xi Li, Yuan-Hua Chen, Dong-Dong Xie

“…Mechanically, NaAsO2 promoted GSK-3β inactivation in the ''disruption complex'' through Akt- mediated phosphorylation at serine 9, and then inhibited the phosphorylation and ubiquitination degradation of β-catenin, which led to its nuclear translocation. …”
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The histone demethylase KDM5C enhances the sensitivity of acute myeloid leukemia cells to lenalidomide by stabilizing cereblon by Lu Zou, Dan Cao, Qing Sun, Wenjun Yu, Bingzong Li, Guoqiang Xu, Liang Zhou

“…Len binds to CRBN, recruits IKZF1/IKZF3, and promotes their ubiquitination and degradation, through which Len exhibits its antileukemia and antimyeloma activity. …”
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UBE2T/CDC42/CD276 signaling axis mediates brain metastasis of triple-negative breast cancer via lysosomal autophagy by Lin Li, Rong Fu, Yan Chen, Jin Yao, Xiaobing Jiang, Tao Liang, Jun jun Li, Qian zhi Chen, Liang Liang Shi, Ming Xing Xie, Xin Wei Qiao, Qi Hong Cheng, Min Jie Wang

“…Finally, animal experimental results also showed that inhibition of UBE2T elevated the TNBC sensitivity to immune checkpoint CD276 blockade and inhibited BrM of TNBC.Conclusions In conclusion, our results indicate a new mechanism whereby UBE2T-mediated ubiquitination positively controls the UBE2T/CDC42/CD276 axis to upregulate tumor cell expression of CD276 and thereby impair CD8+ T cells function, ultimately leading to tumor cell immune escape and BrM.…”
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GC-derived exosomal circMAN1A2 promotes cancer progression and suppresses T-cell antitumour immunity by inhibiting FBXW11-mediated SFPQ degradation by Yikai Shen, Jie Lin, Tianlu Jiang, Xusheng Shen, Ying Li, Yiwang Fu, Penghui Xu, Lang Fang, Zetian Chen, Hongxin Huang, Yiwen Xia, Zekuan Xu, Linjun Wang

“…Mechanistically, circMAN1A2 competed with FBXW11 for binding to SFPQ, preventing FBXW11-mediated k48-linked ubiquitination and SFPQ protein degradation, thereby stabilizing SFPQ expression. …”
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Epigenetics disruptions enabled by porphyrin-derived metal-organic frameworks disarm resistances to sonocatalytic ROS anti-tumor actions by Yan Zhang, Dou Du, Chao Fang, Xin Yu, Yujia Fang, Xinyu Liu, Di Ou, Haohao Yin, Hui Liu, Taixia Wang, Lu Lu, Xiaolong Li, Kun Zhang

“…Herein, we proposed an epigenetic deubiquitination disruption strategy to disarm the ubiquitination-deubiquitination balance-induced resistances to ROS production and ROS-based anti-tumor action for potentiating sonodynamic treatment (SDT) efficiency. …”
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A structure-based designed small molecule depletes hRpn13Pru and a select group of KEN box proteins by Xiuxiu Lu, Monika Chandravanshi, Venkata R. Sabbasani, Snehal Gaikwad, V. Keith Hughitt, Nana Gyabaah-Kessie, Bradley T. Scroggins, Sudipto Das, Wazo Myint, Michelle E. Clapp, Charles D. Schwieters, Marzena A. Dyba, Derek L. Bolhuis, Janusz W. Koscielniak, Thorkell Andresson, Michael J. Emanuele, Nicholas G. Brown, Hiroshi Matsuo, Raj Chari, Deborah E. Citrin, Beverly A. Mock, Rolf E. Swenson, Kylie J. Walters

“…Here, we show that XL44 induces ubiquitin-dependent loss of hRpn13Pru and ubiquitin-independent loss of select KEN box containing proteins.…”
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Frk positively regulates innate antiviral immunity by phosphorylating TBK1 by Xiaomei Zhang, Ying You, Tingrong Xiong, Tingrong Xiong, Xiaokai Zhang, Haibo Wang, Jinxia Geng, Miao Wang, Yanyan Xu, Shanshan Gao, Shanshan Gao, Xiaoyan Wu, Yue Zheng, Xianhua Wen, Haoyu Yang, Yu Wang, Yu Wang, Xiaohua Wen, Congcong Zhao

“…Mechanistically, Frk promotes the K63 ubiquitination of TBK1 and subsequent activation of the transcription factor IRF3 by phosphorylating TBK1 at tyrosine residues 174 and 179, thereby enhancing the production of IFN-β in macrophages. …”
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Synaptotagmin-1 attenuates myocardial programmed necrosis and ischemia/reperfusion injury through the mitochondrial pathway by Teng Sun, Jialei Li, Shuang Wang, Yu Han, Xiangyu Tao, Min Yuan, Zhijie Jing, Ting Liu, Yuehong Qi, Siqi Liu, Yanlin Feng, Jiasong Chang, Lan Zhou, Lijuan Gao, Jianyun Shi, Ruihong Ning, Jimin Cao

“…In exploring the underlying mechanisms, we found that Syt1 interacted with Parkin and promoted Parkin-catalyzed CypD ubiquitination, thus inhibited mitochondrial membrane permeability transition pore (mPTP) opening and ultimately suppressed cardiomyocyte necrosis. …”
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