Overexpression of the Synthetic Chimeric Native-T-phylloplanin-GFP Genes Optimized for Monocot and Dicot Plants Renders Enhanced Resistance to Blue Mold Disease in Tobacco (N. taba... by Dipak K. Sahoo, Sumita Raha, James T. Hall, Indu B. Maiti

“…Transgenic plants were morphologically similar to wild-type plants and showed no deleterious effects due to transgene expression. …”
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Overcoming the Silencing of Doxycycline-Inducible Promoters in hiPSC-derived Cardiomyocytes [version 1; peer review: 1 approved, 2 approved with reservations] by Michelle Guichardaz, Elisa Balmas, Alessandro Bertero, Sveva Bottini

“…Effective control of transgene expression is crucial to achieve temporal and quantitative precision in all of these contexts. …”
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Vectorized Treg-depleting αCTLA-4 elicits antigen cross-presentation and CD8+ T cell immunity to reject ‘cold’ tumors by Laetitia Fend, Eric Quemeneur, Monika Semmrich, Jean-Baptiste Marchand, Matilda Rehn, Nathalie Silvestre, Linda Mårtensson, Johann Foloppe, Ingrid Teige, Björn Frendeus, Petra Holmkvist, Carolin Svensson, Christelle Remy, Patricia Kleinpeter, Jules Deforges, Fred Junghus, Kirstie L Cleary, Mimoza Bodén

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Neuroinflammation in Parkinson's Disease and Related Disorders: A Lesson from Genetically Manipulated Mouse Models of α-Synucleinopathies by Kazunari Sekiyama, Shuei Sugama, Masayo Fujita, Akio Sekigawa, Yoshiki Takamatsu, Masaaki Waragai, Takato Takenouchi, Makoto Hashimoto

“…To better understand neuroinflammation in PD, we focused on glial activation in α-synuclein (αS) transgenic and related model mice. In the majority of αS transgenic mice, astrogliosis was observed concomitantly with accumulation of αS during the early stage of neurodegeneration. …”
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Genetic Rat Models of Parkinson's Disease by Ryan M. Welchko, Xavier T. Lévêque, Gary L. Dunbar

“…Of the genes that have been linked to PD, α-synuclein and leucine-rich repeat kinase 2 have been used to develop transgenic rat models of the disease. In this paper we focused on the various transgenic rat models of PD in terms of their ability to mimic key symptoms of PD in a progressive manner. …”
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Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection by José M. Alvarez, Ricardo J. Ordás

“…Transgenic plants were transferred to potting substrate showing normal growth.…”
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DNA Damage and Augmented Oxidative Stress in Bone Marrow Mononuclear Cells from Angiotensin-Dependent Hypertensive Mice by Bianca P. Campagnaro, Clarissa L. Tonini, Breno V. Nogueira, Dulce E. Casarini, Elisardo C. Vasquez, Silvana S. Meyrelles

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AAV-based vectors for human diseases modeling in laboratory animals by Timur I. Aliev, Dmitry V. Yudkin

“…The development of therapeutic drugs and vaccines requires the availability of appropriate model animals that replicate the pathogenesis of human diseases. Both native and transgenic animals can be utilized as models. The advantage of transgenic animals lies in their ability to simulate specific properties desired by researchers. …”
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Osteoblast-Specific Overexpression of Nucleolar Protein NO66/RIOX1 in Mouse Embryos Leads to Osteoporosis in Adult Mice by Qin Chen, Krishna M. Sinha, Benoit de Crombrugghe, Ralf Krahe

“…Here, we generated osteoblast-specific transgenic mice overexpressing a FLAG-tagged NO66 transgene driven by the 2.3 kB alpha-1type I collagen (Col1a1) promoter. …”
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A dual-RPA based lateral flow strip for sensitive, on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops by Jinbin Wang, Yu Wang, Xiuwen Hu, Yifan Chen, Wei Jiang, Xiaofeng Liu, Juan Liu, Lemei Zhu, Haijuan Zeng, Hua Liu

“…Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient. …”
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The insertion of an ancestral gene in Nicotiana tabacum plants reduces free radicals during saline irrigation by Claudia Marissa Calderón Torres, Victoria Abril Mancilla Galván, Miguel Murguía Romero

“…Transgenic organisms modified with ancestral genes for nitrogen metabolism are rare. …”
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α-Copaene is a potent repellent against the Asian Citrus Psyllid Diaphorina citri by Rodrigo Facchini Magnani, Haroldo Xavier Linhares Volpe, Rejane Angélica Grigio Luvizotto, Tatiana Aparecida Mulinari, Thiago Trevisoli Agostini, Jairo Kenupp Bastos, Victor Pena Ribeiro, Michele Carmo-Sousa, Nelson Arno Wulff, Leandro Peña, Walter S. Leal

“…Species that pull ACP away from commercial citrus plants have been identified, and transgenic plants that repel ACP have been developed. …”
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Transcriptional Targeting of Mature Dendritic Cells with Adenoviral Vectors via a Modular Promoter System for Antigen Expression and Functional Manipulation by Ilka Knippertz, Andrea Deinzer, Jan Dörrie, Niels Schaft, Dirk M. Nettelbeck, Alexander Steinkasserer

“…To specifically target dendritic cells (DCs) to simultaneously express different therapeutic transgenes for inducing immune responses against tumors, we used a combined promoter system of adenoviral vectors. …”
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APOE Christchurch enhances a disease-associated microglial response to plaque but suppresses response to tau pathology by Kristine M. Tran, Nellie E. Kwang, Claire A. Butler, Angela Gomez-Arboledas, Shimako Kawauchi, Cassandra Mar, Donna Chao, Rocio A. Barahona, Celia Da Cunha, Kate I. Tsourmas, Zechuan Shi, Shuling Wang, Sherilyn Collins, Amber Walker, Kai-Xuan Shi, Joshua A. Alcantara, Jonathan Neumann, Duc M. Duong, Nicholas T. Seyfried, Andrea J. Tenner, Frank M. LaFerla, Lindsay A. Hohsfield, Vivek Swarup, Grant R. MacGregor, Kim N. Green

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Analysis of the structure and function of the tomato <i>Solanum lycopersicum</i> L. MADS-box gene <i>SlMADS5</i> by A. V. Nezhdanova, M. A. Slugina, E. A. Dyachenko, A. M. Kamionskaya, E. Z. Kochieva, A. V. Shchennikova

“…Analysis of the expression of SlMADS5 and the tobacco genes NtLFY, NtAP1, NtWUS, NtAG, NtPLE, NtSEP1, NtSEP2, and NtSEP3 in leaves and apexes of transgenic and control plants showed that SlMADS5 mRNA is present only in tissues of transgenic lines. …”
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In Vivo Optical Imaging of Neurogenesis: Watching New Neurons in the Intact Brain by Sebastien Couillard-Despres, Rudolf Finkl, Beate Winner, Sonja Ploetz, Dirk Wiedermann, Robert Aigner, Ulrich Bogdahn, Juergen Winkler, Mathias Hoehn, Ludwig Aigner

“…We developed a new tool based on the doublecortin promoter driving the expression of the luciferase reporter gene (DCX-promo-luciferase) in transgenic mice to perform in vivo imaging of neurogenesis. …”
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Novel Roles of the Picornaviral 3D Polymerase in Viral Pathogenesis by Jason Kerkvliet, Ramakrishna Edukulla, Moses Rodriguez

“…The RNA-dependent RNA-polymerase, 3Dpol, is an essential component in the picornavirus genome for the replication of single stranded RNA. However, transgenic expression of 3Dpol in mice has antiviral effects. …”
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Effectiveness of B-actin promoter on driving target gene expression in common carp transgenesis by Andi Aliah Hidayani, Odang Carman, . Alimuddin

“… Promoter in transgene construct plays an important role on regulating of transgene expression level in transgenic fish. …”
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Homozygous mice with mutant protein FUS[1-359] overexpression: Innovative possibilities for ALS treatment by Nikita S. Zhunusov

“…Materials and Methods: The study utilized tg_hFUS[1-359] animal lines with a transgenic cassette expressing the human mutant FUS[1-359] protein. …”
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Efficient chimeric mouse production using a novel embryonic stem cell line by G. V. Kontsevaya, N. A. Feofanova, A. G. Menzorov, I. E. Pristyazhnyuk, A. V. Smirnov, N. R. Battulin, L. A. Gerlinskaya

“…Embryonic stem cells are commonly used for generation of transgenic mice. Embryonic stem cells could participate in the development of chimeric animals after injection into a blastocyst. …”
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