Fc-binding nanodisc restores antiviral efficacy of antibodies with reduced neutralizing effects against evolving SARS-CoV-2 variants by Jaehyeon Hwang, Soyun Choi, Beom Kyu Kim, Sumin Son, Jeong Hyeon Yoon, Kyung Won Kim, Wonbeom Park, Hyunjoo Choo, Suhyun Kim, Soomin Kim, Seokhyeon Yu, Sangwon Jung, Sang Taek Jung, Min-Suk Song, Sang Jick Kim, Dae-Hyuk Kweon

“…Furthermore, the Fc-binding nanodisc significantly boosted the antiviral efficacy of the soluble angiotensin-converting enzyme 2 (sACE2) Fc fusion protein, which possesses broad but modest antiviral activity. In ACE2 transgenic mice, the Fc-binding nanodisc protected better than sACE2-Fc alone with two more log reduction in lung viral titer. …”
Get full text

Synaptic modulation of glutamate in striatum of the YAC128 mouse model of Huntington disease by Judy Cheng, Ellen T. Koch, Daniel Ramandi, James P. Mackay, Timothy P. O’Leary, William Rees-Jones, Lynn A. Raymond

“…Thus, we used corticostriatal brain slices and optogenetic probes to directly investigate neuromodulatory signaling in the transgenic YAC128 HD mouse model. Results: Low-dose D2 agonist quinpirole reduced cortically-evoked glutamate release in dorsal striatum of premanifest YAC128 slices but not WT, and blocking type 1 cannabinoid receptors mitigated this effect. …”
Get full text

Clinical and Functional Characteristics of a Novel KLF11 Cys354Phe Variant Involved in Maturity-Onset Diabetes of the Young by Yujing Sun, Jingru Qu, Jing Wang, Ruxing Zhao, Chuan Wang, Li Chen, Xinguo Hou

“…Cell-based luciferase reporter assays using wild-type and mutant transgenes revealed that the KLF11 (c.1061G > T) variant had impaired insulin promoter regulation activity. …”
Get full text

Optimized framework for evaluating F3 transgressive segregants in cayenne pepper by Ifayanti Ridwan, Muh Farid, Feranita Haring, Nuniek Widiayani, Ahmad Yani, Nirwansyah Amier, Muhammad Alfan Ikhlasul Amal, Jekvy Hendra, Nawab Ali, Mekhled Mohamed Alenazi, Mahmoud F. Seleiman, Willy Bayuardi Suwarno, Muhammad Fuad Anshori

“…Abstract Developing F3 transgenic segregants has significant potential to improve cayenne pepper varieties. …”
Get full text

Design of a humanized CD40 agonist antibody with specific properties using AlphaFold2 and development of an anti-PD-L1/CD40 bispecific antibody for cancer immunotherapy by Kun Du, He Huang

“…Furthermore, in humanized transgenic mice challenged with huPD-L1-expressing tumor cells, BA4415 induced superior anti-tumor activity. …”
Get full text

The Anti-Inflammatory Effects of Formononetin, an Active Constituent of <i>Pueraria montana</i> Var. <i>Lobata</i>, via Modulation of Macrophage Autophagy and Polarization by Linyi Xu, Shuo Zhou, Jing Li, Wenbo Yu, Wenyi Gao, Haoming Luo, Xiaoxue Fang

“…The results of an in vitro anti-inflammatory activity screening assay showed that compounds <b>1</b>, <b>4</b>, <b>6</b>, <b>8</b>, and <b>15</b> were able to significantly reduce the levels of pro-inflammatory cytokines IL-6 and IL-1β in LPS-induced RAW264.7 macrophages, with the most obvious effect produced by compound <b>6</b> (formononetin), while formononetin was able to significantly reduce the number of macrophages at the site of inflammation in transgenic zebrafish. In addition, network pharmacological analysis revealed that the anti-inflammatory activity of formononetin is closely related to autophagy and polarization targets such as <i>TNF</i>, <i>EGFR</i>, <i>PTGS2</i>, and <i>ESR1</i>. …”
Get full text

RNA‐Binding Protein Hnrnpa1 Triggers Daughter Cardiomyocyte Formation by Promoting Cardiomyocyte Dedifferentiation and Cell Cycle Activity in a Post‐Transcriptional Manner by Chuling Li, Yijin Chen, Qiqi Chen, Haoxiang Huang, Michael Hesse, Yilin Zhou, Ming Jin, Yu Liu, Yifei Ruan, Xiang He, Guoquan Wei, Hao Zheng, Senlin Huang, Guojun Chen, Wangjun Liao, Yulin Liao, Yanmei Chen, Jianping Bin

“…With the use of α‐MHC‐H2B‐mCh/CAG‐eGFP‐anillin transgenic mice, Hnrnpa1 overexpression promoted CM DACCA, thereby triggering daughter CM formation and enhancing cardiac repair after myocardial infarction (MI). …”
Get full text

Cerebellar lipid dysregulation in SCA3: A comparative study in patients and mice by Alexandra F. Putka, Varshasnata Mohanty, Stephanie M. Cologna, Hayley S. McLoughlin

“…Lipid downregulation was recapitulated in early- to mid-stage mouse models of SCA3, including transgenic YACQ84 and Knock-in Q300 mice. End-stage Knock-in Q300 mice displayed a progressive reduction in lipid content, highlighting targets that could benefit from early therapeutic intervention. …”
Get full text

T cell receptors specific for an imatinib-induced mutation in BCR-ABL for adoptive T cell therapy by Meng-Tung Hsu, Gerald Willimsky, Gerald Willimsky, Gerald Willimsky, Leo Hansmann, Leo Hansmann, Leo Hansmann, Leo Hansmann, Thomas Blankenstein

“…Two candidate peptides derived from the E255V and the T315I mutation (designated ABL-E255V and ABL-T315I) were selected for study based on their in silico predicted binding affinity to HLA-A2. By immunizing transgenic mice that express a diverse human T cell receptor (TCR) repertoire restricted to HLA-A2, we detected CD8+ T cell responses against the ABL-E255V, but not the ABL-T315I peptide. …”
Get full text

Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms by Mie Suzuki Okutani, Shinya Okamura, Tang Gis, Hitomi Sasaki, Suni Lee, Akiho Kashiwabara, Simon Goto, Mai Matsumoto, Mayuko Yamawaki, Toshiaki Miyazaki, Tatsuya Nakagawa, Masahito Ikawa, Wataru Kamitani, Shiro Takekawa, Koichi Yamanishi, Hirotaka Ebina

“…Furthermore, we confirmed the safety of BK2102 using transgenic (Tg) mice expressing human ACE2 (hACE2) that are highly susceptible to SARS-CoV-2. …”
Get full text

Host genetics and gut microbiota influence lipid metabolism and inflammation: potential implications for ALS pathophysiology in SOD1G93A mice by Elena Niccolai, Leandro Di Gloria, Maria Chiara Trolese, Paola Fabbrizio, Simone Baldi, Giulia Nannini, Cassandra Margotta, Claudia Nastasi, Matteo Ramazzotti, Gianluca Bartolucci, Caterina Bendotti, Giovanni Nardo, Amedeo Amedei

“…Using 16 S rRNA sequencing and fecal metabolite analysis, we characterized the GM composition and metabolite profiles in non-transgenic (Ntg) and SOD1G93A mutant mice of both strains. …”
Get full text

Effects of Ginsenoside Rg1 Regulating Wnt/β-Catenin Signaling on Neural Stem Cells to Delay Brain Senescence by Yue Xiang, Shun-he Wang, Lu Wang, Zi-ling Wang, Hui Yao, Lin-bo Chen, Ya-ping Wang

“…The whole brain of Nestin-GFP transgenic newborn rat was extracted, and NSCs were extracted and cultured to P3 generation. …”
Get full text

Effectiveness of Contrast Agents and Molecular Indicators in Alzheimer's Diagnosis Through PET and SPECT Imaging on Animal Models by Yasaman Abaszadeh, Ramin Ardalani, Athena Dehghan Najm Abadi

“…The most often employed transgenic mouse strains in the studies were APP/PS1, and 5XFAD. …”
Get full text

PSMA antibody, humanized PSMA.CAR10.3, or Cetuximab increases prostate cancer localization of NF-κB p50-deficient immature myeloid cells (p50-IMC) and phagocytosis by their macroph... by Mohammad A. Alzubi, Theresa Barberi, Alan D. Friedman

“…Lack of tolerance of human PSMA or EGFR by immune-competent mice and lack of expression of human PSMA protein in the prostate of AR2-Probasin-hPSMA transgenic mice precluded our ability to determine whether human-specific PSMA or EGFR antibody or PSMA.CAR10.3 increases anti-tumor efficacy of murine p50-IMC. …”
Get full text

Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice. by Yuting Sun, Tingyu Zhou, Silin Ye, Effie Yin Tung Lau, Yao Zeng, Jessie Qiaoyi Liang

“…Furthermore, these methods can be extended to allow genotyping of other SNVs and can facilitate non-invasive genotyping of transgenic animals.…”
Get full text

The Inhibitory Effects of Alpha 1 Antitrypsin on Endosomal TLR Signaling Pathways by Ahmed S. Elshikha, Georges Abboud, Rigena Avdiaj, Laurence Morel, Sihong Song

“…Our in vivo studies using hAAT transgenic mice also showed that hAAT attenuated R848-induced pathogenesis. …”
Get full text

[18F]-(2S,4R)4-Fluoroglutamine PET Imaging of Glutamine Metabolism in Murine Models of Hepatocellular Carcinoma (HCC) by Youngho Seo, Miranda C. Craig, Stephanie T. Murphy, Jinjin Feng, Xin Chen, Mariia Yuneva

“…For this study, we used two transgenic mouse models of HCC induced by protooncogenes, MYC, and MET. …”
Get full text

Crop genes modified using CRISPR/Cas system by A. M. Korotkova, S. V. Gerasimova, V. K. Shumny, E. K. Khlestkina

“…In these studies, the ability to get transgene-free modified plants was widely demonstrated. …”
Get full text

The impact of spray-induced gene silencing on cereal phyllosphere microbiota by Poorva Sundararajan, Samrat Ghosh, Bekele Gelena Kelbessa, Stephen C. Whisson, Mukesh Dubey, Aakash Chawade, Ramesh Raju Vetukuri

“…This has the advantage of avoiding transgenic approaches, but several aspects of the technology require further development to make it a viable field-level management tool. …”
Get full text

Study on the Establishment of Efficient Leaf Regeneration System in ‘<i>Yuluxiang</i>’ Pear by Xiaohua Cheng, Weilong Zhang, Yarui Wei, Yuxing Zhang

“…However, it exhibits a low leaf regeneration frequency of only 35.0%, which hinders its transgenic breeding process. To establish an efficient regeneration system, we utilized tissue culture seedlings of the ‘<i>Yuluxiang</i>’ pear and investigated various factors influencing leaf regeneration: plant growth regulators, natural organic materials, leaf wounding and positioning methods, duration of dark culture, ages and lines of plantlets, as well as culture containers. …”
Get full text