Diabetes insipidus and Guillain-Barré-like syndrome following CAR-T cell therapy: a case report by Christian Koch, Patrick Roth, Juliane Fleischer, Todor Popov, Karl Frontzek, Bettina Schreiner, Markus G. Manz, Simone Unseld, Antonia M. S. Müller, Norman F. Russkamp

“…T cell-rich infiltrates and detection of the CAR transgene in muscle-nerve sections imply a direct or indirect role of CAR-T cell-mediated inflammation. …”
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Transcription factor DREF regulates expression of the microRNA gene <i>bantam in Drosophila melanogaster</i> by M. B.  Schwartz, M. M. Prudnikova, O. V. Andreenkov, E. I. Volkova, I. F. Zhimulev, O. V. Antonenko, S.  A. Demakov

“…In this study, we found that the bantam regulatory region contains motifs characteristic of binding sites for DREF, a transcription factor that regulates the expression of Hippo cascade genes. Using transgenic lines containing a fulllength bantam lethality-rescuing deletion fragment and a fragment with a disrupted DREF binding site, we show that these motifs are functionally significant because their disruption at the bantam locus reduces expression levels in the larvae and ovaries of homozygous flies, which correlates with reduced vitality and fertility. …”
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Curcumin reverses cognitive deficits through promoting neurogenesis and synapse plasticity via the upregulation of PSD95 and BDNF in mice by Gaifen Li, Qiong Wu, Chao Wang, Pin Deng, Jiaxin Li, Zhiguang Zhai, Yubo Li

“…Immunofluorescence assays, including Fluoro-Jade C (FJC) and bromodeoxyuridine (BrdU), were used to detect neuronal degeneration and neurogenesis. Thy1-YFP transgenic mice were used to detect synapse plasticity. …”
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Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice... by Wenqi Wang, Yidi Qu, Siyu Li, Jinyu Chu, Hongxin Yang, Lirong Teng, Di Wang

“…In this study, the neuroprotective effect of CCG through oxidative stress mediated mitochondrial dysfunction on L-glutamate (L-Glu)-damaged hippocampal neuron cell line (HT22) and APPswe/PSEN1dE9 transgenic (APP/PS1) mice were investigated. Observably, CCG in L-Glu-damaged HT22 cells suppressed apoptosis, reduced the accumulation of reactive oxygen species, balanced the mitochondrial membrane potential and prevented the over-influx of calcium. …”
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Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation by Beenzu Siamalube, Emmanuel Ehinmitan, Maina Ngotho, Justus Onguso, Steven Runo

“…Agrobacterium-mediated gene transformation method is a vital molecular biology technique employed to develop transgenic plants. Plants are genetically engineered to develop disease-free varieties, knock out unsettling traits for crop improvement, or incorporate an antigenic protein to make the plant a green factory for edible vaccines. …”
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Chronic Gastroesophageal Reflux Dysregulates Proteostasis in Esophageal Epithelial CellsSummary by Kodisundaram Paulrasu, Ravindran Caspa Gokulan, Wael El-Rifai, Zhibin Chen, Jianwen Que, Timothy C. Wang, Olivier G. Boutaud, Karoline Briegel, Sergey I. Dikalov, Monica T. Garcia-Buitrago, Alexander I. Zaika

“…Surgical model of esophageal reflux injury and L2-IL1β transgenic mice were used to investigate the mechanisms of protein misfolding and aggregation. …”
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Establishment of a Novel Model for Anticancer Drug Resistance in Three-Dimensional Primary Culture of Tumor Microenvironment by Tatsuya Usui, Masashi Sakurai, Shuhei Enjoji, Hideyoshi Kawasaki, Koji Umata, Takashi Ohama, Nobuyuki Fujiwara, Ryotaro Yabe, Shunya Tsuji, Hideyuki Yamawaki, Shoichi Hazama, Hiroko Takenouchi, Masao Nakajima, Ryouichi Tsunedomi, Nobuaki Suzuki, Hiroaki Nagano, Koichi Sato

“…As a three-dimensional tumor microenvironment model, air liquid interface (ALI) organoid culture from oncogene transgenic mouse gastrointestinal tissues was recently produced. …”
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Methodical approaches in experimental modelling of non-alcoholic fatty liver disease by N. V. Bivalkevich, Yu. K. Denisenko, T. P. Novgorodtseva

“…Genetic models of NAFLD developing on background of obesity and metabolicsyndrome represent the second category (animals, with leptin gene deletion – ob/ob, mice resistant to leptin action, – db/db and transgenic mice with excessive expression of styrene-regulating element of protein-1 isoform. …”
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Prx1-Expressing Progenitor Primary Cilia Mediate Bone Formation in response to Mechanical Loading in Mice by Emily R. Moore, Julia C. Chen, Christopher R. Jacobs

“…We then traced Prx1 progenitor lineage using a transgenic mouse model carrying both a Prx1-driven tamoxifen-inducible Cre and a ROSA-driven lacZ reporter gene. …”
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Proliferating CLL cells express high levels of CXCR4 and CD5 by Daniel Friedman, Drshika P. Mehtani, Jennifer B. Vidler, Piers E. M. Patten, Robbert Hoogeboom

“…Dye dilution experiments revealed an accumulation of Ki67hi‐divided cells in the CXCR4hiCD5hi fraction. In Eµ‐TCL1 transgenic mice, the CXCR4hiCD5hi fraction expressed high levels of ki67 and was expanded in enlarged spleens of diseased animals. …”
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CD22 modulation alleviates amyloid β-induced neuroinflammation by Yu Dong Mai, Qingqing Zhang, Cheuk Lim Fung, Shui On Leung, Chi Ho Chong

“…The pivotal therapeutic potential of targeting CD22 was demonstrated in Amyloid β (Aβ) induced-neuroinflammation in hCD22 transgenic mice. Suciraslimab improved working memory and resolved neuroinflammation in vivo. …”
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Protocol to Identify Unknown Flanking DNA Using Partially Overlapping Primer-based PCR for Genome Walking by Mengya Jia, Dongqin Ding, Xiaohua Liu, Haixing Li

“…This POP-PCR protocol has many potential applications, such as screening microbes, identifying transgenic sites, or mining new genetic resources.…”
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Differential bone and vessel type formation at superior and dura periosteum during cranial bone defect repair by Yuankun Zhai, Zhuang Zhou, Xiaojie Xing, Mark Nuzzle, Xinping Zhang

“…While the distinct embryonic origins of the superior and dura periosteum of the cranial parietal bone have been described, the extent of their respective contributions to bone and vessel formation during adult bone defect repair remains superficially explored. Utilizing transgenic mouse models in conjunction with high-resolution multiphoton laser scanning microscopy (MPLSM), we have separately evaluated bone and vessel formation in the superior and dura periosteum before and after injury, as well as following intermittent treatment of recombinant peptide of human parathyroid hormone (rhPTH), Teriparatide. …”
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Alterations in mRNA level of proteins related to redox state and mitochondria in an Alzheimer’s disease animal model: Promising targets in neuroprotection by Sylwia Żulińska, Przemysław L. Wencel, Kinga Czubowicz, Joanna B. Strosznajder

“…Moreover, transcription of genes related to mitochondrial electron transport complexes (ETC) and biogenesis in the brain cortex of 4-, 6- and 12-month-old transgenic AD Tg mice was analyzed. We observed significant decreases in mRNA of Sod2, Parp1 and Sirt1 in the 3-month-old AD Tg mice and upregulation of Parp1 in the 6-month-old AD Tg mice by qPCR analysis. …”
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Induction of translation-suppressive G3BP1+ stress granules and interferon-signaling cGAS condensates by transfected plasmid DNA by Vladimir Majerciak, Zhi-Ming Zheng

“…Inhibition of SG formation mediated by PKR KD, but not cGC KD, also led to increased expression of transgenes, indicating that PKR activation represents a major roadblock to gene expression. …”
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Role of the Kaiso gene in the development of inflammation in Mucin-2 defcient mice by E. A. Litvinova, K. M. Achasova, M. A. Borisova, S. V. Zhenilo, E. B. Prokhortchouk, E. N. Kozhevnikova

“…The most popular model is considered to be transgenic models in which individual genes are knocked out. …”
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Magnetic resonance imaging in primates. The example of the mouse lemur (Microcebus murinus): From detection of pathological aging to therapeutic evaluations by Nelly Joseph-Mathurin, Olene Dorieux, Audrey Kraska, Anne Bertrand, Mathieu Santin, Nadine El Tannir El Tayara, Marc Dhenain

“…Rodents and particularly transgenic mice are widely used as models for research on physiopathology of cerebral aging, neurodegenerative diseases and for the evaluation of therapies. …”
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A simple and reliable method for claustrum localization across age in mice by Tarek Shaker, Gwyneth J. Dagpa, Vanessa Cattaud, Brian A. Marriott, Mariam Sultan, Mohammed Almokdad, Jesse Jackson

“…Methods based on gene/protein expression or transgenic mice have been used to spatially outline the claustrum but often report incomplete labeling and/or lack of specificity during certain neurodevelopmental timepoints. …”
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Cardiac-Specific Gene Expression Facilitated by an Enhanced Myosin Light Chain Promoter by Wolfgang Boecker, Oliver Y. Bernecker, Joseph C. Wu, Xinsheng Zhu, Tomohiro Sawa, Luanda Grazette, Anthony Rosenzweig, Federica del Monte, Ulrich Schmidt, Roger J. Hajjar

“…A major limitation of myocardial gene therapy is the extracardiac transgene expression. Methods: To minimize extracardiac gene expression, we have constructed a tissue-specific promoter for cardiac gene transfer, namely, the 250-bp fragment of the myosin light chain-2v (MLC-2v) gene, which is known to be expressed in a tissue-specific manner in ventricular myocardium followed by a luciferase (luc) reporter gene (Ad.4 × MLC 250 .Luc). …”
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A Mutation in IL4RA Is Associated with the Degree of Pathology in Human TB Patients by Christoph Hölscher, Lisa Heitmann, Ellis Owusu-Dabo, Rolf D. Horstmann, Christian G. Meyer, Stefan Ehlers, Thorsten Thye

“…To evaluate whether the human-like TB pathology in IL-13-overexpressing mice is specifically mediated through the IL-4Rα subunit, we analyzed IL-13 transgenic mice with a genetic ablation of the IL-4Rα. …”
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