Comparison of natural and artificial vasopressin deficiency: why the latter is lethal? by D. Zelena

“…The transgenic mouse technology is widespread, however, untill now 22.0 % of tested null mutations was found to be lethal. …”
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Blueprint for a high-performance biomaterial: full-length spider dragline silk genes. by Nadia A Ayoub, Jessica E Garb, Robin M Tinghitella, Matthew A Collin, Cheryl Y Hayashi

“…For this reason, the mass-production of artificial spider silks through transgenic technologies has been a major goal of biomimetics research. …”
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A comprehensive review of biological and genetic control approaches for leishmaniasis vector sand flies; emphasis towards promoting tools for integrated vector management. by Yasoda Kumari, Nayana Gunathilaka, Deepika Amarasinghe

“…This current review identified several promising methods, including genetically modified sand flies, using transgenic approaches by taking advanced gene editing tools like Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9) and genetic modification of symbiotic microorganisms for controlling sand fly populations, which appeared to be proven under laboratory and field settings.…”
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Functional divergence caused by ancient positive selection of a Drosophila hybrid incompatibility locus. by Daniel A Barbash, Philip Awadalla, Aaron M Tarone

“…Several Drosophila melanogaster genes involved in hybrid incompatibility have been identified but none has yet been shown to have functionally diverged in accordance with the Dobzhansky-Muller model. By introducing transgenic copies of the X-linked Hybrid male rescue (Hmr) gene into D. melanogaster from its sibling species D. simulans and D. mauritiana, we demonstrate that Hmr has functionally diverged to cause F1 hybrid incompatibility between these species. …”
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Adeno-Associated virus 8 delivers an immunomodulatory peptide to mouse liver more efficiently than to rat liver. by Yuqing Wang, Ayrea Hurley, Marco De Giorgi, Mark R Tanner, Rong-Chi Hu, Michael W Pennington, William R Lagor, Christine Beeton

“…We designed AAV8 vectors expressing the target transgene, ShK-235, or Enhanced Green fluorescent protein (EGFP). …”
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Effect of fecal microbiota transplantation on ulcerative colitis model in rats: The gut–brain axis by Mahdis Morshedbak, Kaveh Rahimi, Mohammad Reza Tabandeh

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Effect of the B chromosome-located long non-coding RNAs on gene expression in maize by Xin Liu, Wenjie Yue, Shiqi Lin, Yuxian Yang, Tong Chen, Xiaowen Shi

“…Using artificial chromosomes in maize breeding allows for site-specific integration of multigene stacks, effectively overcoming the limitations of conventional transgenic approaches. The maize B chromosome, which is dispensable and highly heterochromatic, has minimal impact on phenotypes at low copy numbers, making it a promising platform for engineering artificial chromosomes. …”
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Rabex-5 E3 and Rab5 GEF domains differ in their regulation of Ras, Notch, and PI3K signaling in Drosophila wing development. by Theresa A Reimels, Mia Steinberg, Hua Yan, Sivan Shahar, Ashley Rosenberg, Kristina Kalafsky, Max Luf, Lindsay Kelly, Stacia Octaviani, Cathie M Pfleger

“…Here we report genetic interactions using Rabex-5 transgenes encoding domain-specific mutations that show that Rabex-5 requires an intact E3 domain to inhibit Notch signaling in the epithelial tissue of the developing wing. …”
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Plant thionins: structure, biological functions and potential use in biotechnology by T. I. Odintsova, M. P. Slezina, E. A. Istomina

“…Thionins are toxic to different types of cells including mammalian cancer cell lines. Transgenic plants expressing thionin genes display enhanced resistance to pathogens. …”
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Treatment with Herbal Formula Extract in the hSOD1G93A Mouse Model Attenuates Muscle and Spinal Cord Dysfunction via Anti-Inflammation by Eun Jin Yang, Sun Hwa Lee, Mudan Cai

“…Herbal formula extract (HFE) (1 mg/g) were orally administered once daily for six weeks, starting at eight weeks of age, in hSOD1G93A transgenic mice. To evaluate the effects of HFE, we performed footprint behavioral tests, western blotting, and immunohistochemistry to detect protein expression and quantitative PCR to detect mRNA levels in the muscles and spinal cord of hSOD1G93A mice. …”
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Cardiac-Restricted IGF-1Ea Overexpression Reduces the Early Accumulation of Inflammatory Myeloid Cells and Mediates Expression of Extracellular Matrix Remodelling Genes after Myoca... by Enrique Gallego-Colon, Robert D. Sampson, Susanne Sattler, Michael D. Schneider, Nadia Rosenthal, Joanne Tonkin

“…Taken together, these results indicate that the IGF-1Ea transgene shifts the balance of innate immune cell populations early after infarction, favouring a reduction in inflammatory myeloid cells. …”
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Bone Marrow-Derived Cells Contribute to the Maintenance of Thymic Stroma including TECs by Shami Chakrabarti, Mohammed Hoque, Nawshin Zara Jamil, Varan J. Singh, Daniel Pollacksmith, Neelab Meer, Mark T. Pezzano

“…Grafting of C57BL/6 fetal thymi under the kidney capsule of H2BGFP transgenic mice revealed that peripheral CD45+ EpCAM+ GFP-expressing cells migrate into the developing thymus and contribute to both TECs and FSP1-expressing thymic stroma. …”
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Effects of Metalloporphyrins on Heme Oxygenase-1 Transcription: Correlative Cell Culture Assays Guide in Vivo Imaging by Monica Hajdena-Dawson, Weisheng Zhang, Pamela R. Contag, Ronald J. Wong, Hendrik J. Vreman, David K. Stevenson, Christopher H. Contag

“…To study the safety of these compounds, we assessed their cytotoxic effects and measured luciferase activity by bioluminescent imaging (BLI) as an index of HO-1 transcription, first in live cell cultures and then in living transgenic reporter mice. A total of 12 Mps were first evaluated in the correlative cell culture assay. …”
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Halo-RPD: searching for RNA-binding protein targets in plants by A. O. Shamustakimova

“…The proposed protocol uses plants with stable fusion protein expression and Magne® HaloTag® magnetic beads to capture RNA-protein complexes directly from the cytoplasmic lysate of transgenic Arabidopsis thaliana plants. The key stages described in the paper are as follows: (1) preparation of the magnetic beads; (2) tissue homogenization and collection of control samples; (3) precipitation and wash of RNA-protein complexes; (4) evaluation of protein binding efficiency; (5) RNA isolation; (6) analysis of the RNA obtained. …”
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Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism by Yihua Zhan, Jing Wang, Xuan Zhao, Zhifu Zheng, Yi Gan

“…A total of 9 homozygous T3 generation transgenic lines were obtained. Compared with wild type (WT), overexpression (OE) of AhMAGL3b had no obvious growth inhibition by investigation of agronomic traits, including growth and photosynthetic parameters. …”
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Aplication of Gene Transfer in Aquaculture by . Alimuddin, G. Yoshizaki, O. Carman, K. Sumantadinata

“…One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. This technique has been applied to several commercially valuable species. …”
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Construction and evaluation of Brassica rapa orphan genes overexpression library by Mingliang Jiang, Mingliang Jiang, Zongxiang Zhan, Xiaonan Li, Zhongyun Piao

“…Interestingly, 72.66% of the transgenic lines showed distinctive phenotypic changes. …”
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Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses by Tsheten Sherpa, Tsheten Sherpa, Nrisingha Dey

“…Finally, we tested the efficacy and tissue specificity of the MFH17 promoter in planta by developing transgenic Nicotiana tabacum and Arabidopsis thaliana plants and found it highly constitutive and efficient in driving the gene throughout the plant tissues. …”
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Drought Tolerance in Modern and Wild Wheat by Hikmet Budak, Melda Kantar, Kuaybe Yucebilgili Kurtoglu

“…Prior to their introgression to modern wheat cultivars, drought related candidate genes are first characterized at the molecular level, and their function is confirmed via transgenic studies. After integration of the tolerance loci, specific environment targeted field trials are performed coupled with extensive analysis of morphological and physiological characteristics of developed cultivars, to assess their performance under drought conditions and their possible contributions to yield in certain regions. …”
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A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish. by Ugo Coppola, Bitan Saha, Jennifer Kenney, Joshua S Waxman

“…Mutation of the Lef/Tcf sites within the 3'reg1 reporter, knockdown of Tcf7l1a, and manipulation of canonical Wnt signaling support that Tcf7l1a is derepressed via Wnt signaling to activate the transgenic enhancer and promote AC differentiation. …”
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