Enhancing human ACE2 expression in mouse models to improve COVID‐19 research by Sun Jiaoyang, Cheng Shaofei, Hong Guangliang, Quan Xiongzhi, Lin Haofeng, Mao Rui, Johannes Grillari, Shi Zheng‐Li, Chen Jiekai, Liu Meiqin, Wu Haoyu, Wu Guangming

“…Our study demonstrates the dual regulatory role of β‐globin element in transgene transcription and suggests that opt‐hACE2 mice might serve as valuable tools for SARS‐CoV‐2 research.…”
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Increased RTN3 phenocopies nonalcoholic fatty liver disease by inhibiting the AMPK–IDH2 pathway by Hao Huang, Shuai Guo, Ya‐Qin Chen, Yu‐Xing Liu, Jie‐Yuan Jin, Yun Liang, Liang‐Liang Fan, Rong Xiang

“…We found an increased expression of RTN3 in NAFLD patients, high‐fat diet mice, and oxidized low‐density lipoprotein‐treated L02 cells. The RTN3 transgenic mice exhibited the phenotypes of fatty liver and lipid accumulation. …”
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Trans-kingdom sRNA silencing in the prevention and control of crop Fusarium wilt disease by Xin-Ni Huang, Yue Wang, Yan-Ting Li, Ying Xiao, Shou-Qiang Ouyang

“…Concerning the safety and costly process of generating genetically modified crops, a plant-disease management strategy not reliant on transgenic approaches remains the most economic and environmental-friendly option. …”
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Superficial Dsg2 Expression Limits Epidermal Blister Formation Mediated by Pemphigus Foliaceus Antibodies and Exfoliative Toxins by Donna Brennan, Ying Hu, Walid Medhat, Alicia Dowling, Mỹ G. Mahoney

“…To further study the pathomechanism of these diseases and to assess the adhesive properties of Dsg2, we employed a recently established transgenic (Tg) mouse model expressing Dsg2 in the superficial epidermis. …”
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Comparison of New Tau PET-Tracer Candidates With [F]T808 and [F]T807 by Lieven Declercq Pharmacist, Sofie Celen PhD, Joan Lecina PhD, Muneer Ahamed PhD, Thomas Tousseyn Prof, MD, PhD, Diederik Moechars PhD, Jesus Alcazar PhD, Manuela Ariza PhD, Katleen Fierens PhD, Astrid Bottelbergs PhD, Jonas Mariën PhD, Rik Vandenberghe Prof, MD, PhD, Ignacio Jose Andres PhD, Koen Van Laere Prof, MD, PhD, Alfons Verbruggen Prof, PhD, Guy Bormans Prof, PhD

“…No specific binding of [ 18 F]T808 was found in transgenic mice expressing mutant human P301L tau. In semiquantitative autoradiography studies on human Alzheimer disease slices, we observed more than 50% tau selective blocking of [ 18 F]T808 in the presence of 1 µmol/L of the novel ligands. …”
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Metabolic rate and insulin-independent glucose uptake increase in a TDP-43Q331K mouse model of amyotrophic lateral sclerosis by Tanya S. McDonald, Cedric S. Cui, Titaya Lerskiatiphanich, Jianina Marallag, John D. Lee

“…Mutant TDP-43Q331K mice and non-transgenic controls underwent indirect calorimetry, as well as intraperitoneal glucose, insulin, and glucagon tolerance testing. …”
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From Ca2+ dysregulation to heart failure: β-adrenoceptor activation by RKIP postpones molecular damages and subsequent cardiac dysfunction in mice carrying mutant PLNR9C by correct... by Theresa Brand, Bettina Tanitha Baumgarten, Sabrina Denzinger, Yvonne Reinders, Miriam Kleindl, Constanze Schanbacher, Florian Funk, Nilgün Gedik, Mahmood Jabbasseh, Petra Kleinbongard, Jan Dudek, Julia Szendroedi, Elen Tolstik, Kai Schuh, Martina Krüger, Dobromir Dobrev, Friederike Cuello, Albert Sickmann, Joachim P. Schmitt, Kristina Lorenz

“…However, the underlying molecular mechanisms and the precise contribution of defects in Ca2+-cycling regulation in the development of HF are still not completely resolved. Here, we used transgenic mice that express a human mutation in the cardiomyocyte Ca2+-regulator phospholamban (PLNR9C-tg) causing severe HF due to a reduction in Ca2+ reuptake into the sarco(endo)plasmic reticulum (SR). …”
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Immunopathogenesis of inflammatory bowel diseases by Ye. A. Konovich, I. L. Khalif, M. V. Shapina

“…At genetically sensitive animals inflammatory bowel diseases (IBD) develop at various effects on innate and adaptive systems of immune defense (knock-out and transgenic mice), causing changes of expression of significant immunologic factors with distortion of pro- and anti-inflammatory cells and molecules ratio at their contact to microbiota structures. …”
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Optogenetic Inhibition of Striatal Neuronal Activity Improves the Survival of Transplanted Neural Stem Cells and Neurological Outcomes after Ischemic Stroke in Mice by Yifan Lu, Lu Jiang, Wanlu Li, Meijie Qu, Yaying Song, Xiaosong He, Zhijun Zhang, Guo-Yuan Yang, Yongting Wang

“…NSCs cultured from transgenic mice expressing fluorescent protein were transplanted into the peri-infarct region of the striatum after transient middle cerebral artery occlusion (tMCAO) surgery. …”
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N-Cadherin Upregulation Promotes the Neurogenic Differentiation of Menstrual Blood-Derived Endometrial Stem Cells by Yanli Liu, Fen Yang, Shengying Liang, Qing Liu, Sulei Fu, Zhenyu Wang, Ciqing Yang, Juntang Lin

“…Collectively, our results confirmed the paracrine effect of MenSCs on neuroprotection as well as their potential for transdifferentiation into glial-like cells and demonstrated that N-cad upregulation promotes the neurogenic differentiation of MenSCs, thereby providing support for transgenic MenSC-based therapy for peripheral nerve injury.…”
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Gene Therapy with Endogenous Inhibitors of Angiogenesis for Neovascular Age-Related Macular Degeneration: Beyond Anti-VEGF Therapy by Selwyn M. Prea, Elsa C. Chan, Gregory J. Dusting, Algis J. Vingrys, Bang V. Bui, Guei-Sheung Liu

“…Preclinical studies of gene transfer in a large animal model have provided impressive preliminary results with a number of transgenes. In addition, a clinical trial in patients suffering from advanced neovascular AMD has provided proof-of-concept for successful gene transfer. …”
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A Novel Mouse Model of Alzheimer's Disease with Chronic Estrogen Deficiency Leads to Glial Cell Activation and Hypertrophy by Annik Prat, Maik Behrendt, Edwige Marcinkiewicz, Sebastien Boridy, Ram M. Sairam, Nabil G. Seidah, Dusica Maysinger

“…These chimeric mice exhibit a total FSH-R knockout (FORKO) and carry two transgenes, one expressing the β-amyloid precursor protein (APPsw, Swedish mutation) and the other expressing presenilin-1 lacking exon 9 (PS1Δ9). …”
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Th17 Cell Response in SOD1G93A Mice following Motor Nerve Injury by Allen Ni, Tao Yang, Nichole A. Mesnard-Hoaglin, Rafael Gutierrez, Evan B. Stubbs, Susan O. McGuire, Virginia M. Sanders, Kathryn J. Jones, Eileen M. Foecking, Junping Xin

“…To understand how nerve injury and immune responses may contribute to ALS development, we investigated responses of CD4+ T cell after facial motor nerve axotomy (FNA) at a presymptomatic stage in a transgenic mouse model of ALS (B6SJL SOD1G93A). SOD1G93A mice, compared with WT mice, displayed an increase in the basal activation state of CD4+ T cells and higher frequency of Th17 cells, which were further enhanced by FNA. …”
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Image Processing Techniques for Assessing Contractility in Isolated Adult Cardiac Myocytes by Carlos Bazan, David Torres Barba, Peter Blomgren, Paul Paolini

“…The proposed method can be utilized to evaluate changes in contractile behavior resulting from drug intervention, disease modeling, transgeneity, or other common applications to mammalian cardiocytes.…”
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Genetically modified chickens as bioreactors for protein-based drugs by Shujuan Meng, Aijun Miao, Sen Wu, Sen Wu, Xuguang Du, Xuguang Du, Fei Gao

“…Protein drug production encompasses various methods, among which animal bioreactors are emerging as a transgenic system. Animal bioreactors have the potential to reduce production costs and increase efficiency, thereby producing recombinant proteins that are crucial for therapeutic applications. …”
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Compensatory synaptotagmin-11 expression conceals parkinson’s disease-like phenotypes in parkin knockout mice by Nan Dong, Zhenli Xie, Anqi Wei, Yuxin Yang, Yongning Deng, Xu Cheng, Bianbian Wang, Yang Chen, Yuhao Gu, Jingyu Yao, Yuhao Qin, Chaowen Zheng, Xi Zhang, Yuqing Zhang, Xinjiang Kang, Guoqing Chen, Qiumin Qu, Changhe Wang, Huadong Xu

“…Unfortunately, most of transgenic mouse models fail to manifest pathological changes observed in PD patients, pending the advancement of PD research. …”
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Risedronate Attenuates Podocyte Injury in Phosphate Transporter-Overexpressing Rats by Yohei Asada, Takeshi Takayanagi, Tsukasa Kawakami, Eisuke Tomatsu, Atsushi Masuda, Yasumasa Yoshino, Sahoko Sekiguchi-Ueda, Megumi Shibata, Tomihiko Ide, Hajime Niimi, Eishin Yaoita, Yusuke Seino, Yoshihisa Sugimura, Atsushi Suzuki

“…We have previously reported that type III Pi transporter-overexpressing transgenic (Pit-1 TG) rats manifest phosphate (Pi)-dependent podocyte injury. …”
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Impacts of hexafluoropropylene oxide tetrameric acid (HFPO-TeA) on neurodevelopment and GABAergic signaling in zebrafish larvae by Linlin Wu, Jian Wang, Heyong Ye, Yiyang Yao, Miaoyang Hu, Jie Cheng, Lingcan Kong, Wenwei Liu, Feng Ge

“…Targeted metabolomics and transcriptomics inquiries clarified that HFPO-TeA exposure reduced the levels of the neurotransmitter gamma-aminobutyric acid (GABA) and downregulated the expression of genes related to the GABA pathway. Simultaneously, transgenic zebrafish exhibited that exposure to HFPO-TeA impedes the growth of GABAergic neurons. …”
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Exposure of Mesenchymal Stem Cells to an Alzheimer’s Disease Environment Enhances Therapeutic Effects by Sang Eon Park, Hyeong Seop Kim, Soo Jin Kwon, Min-Jeong Kim, Suk-joo Choi, Soo-young Oh, Gyu Ha Ryu, Hong Bae Jeon, Duk L. Na, Jong Wook Chang

“…Furthermore, treatment with TGF-β reduced Aβ expression in an AD transgenic mouse model. These results highlighted AD environmental preconditioning is a promising strategy to reduce cell death and ubiquitin conjugate levels and maintain the stemness of MSCs. …”
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Differential Expression of -Associated Genes in Autosomal Dominant Polycystic Kidney Disease by Yeon Joo Yook, Yu Mi Woo, Moon Hee Yang, Je Yeong Ko, Bo Hye Kim, Eun Ji Lee, Eun Sun Chang, Min Joo Lee, Sunyoung Lee, Jong Hoon Park

“…To address this question, we presently created Pkd2 knockout and PKD2 transgenic mouse embryo fibroblasts. We used a mouse oligonucleotide microarray to identify messenger RNAs whose expression was altered by the overexpression of the PKD2 or knockout of the Pkd2. …”
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