Dynamic Support Culture of Murine Skeletal Muscle-Derived Stem Cells Improves Their Cardiogenic Potential In Vitro by Klaus Neef, Philipp Treskes, Guoxing Xu, Florian Drey, Sureshkumar Perumal Srinivasan, Tomo Saric, Erastus Nembo, Judith Semmler, Filomain Nguemo, Christof Stamm, Douglas B. Cowan, Antje-Christin Deppe, Maximilian Scherner, Thorsten Wittwer, Jürgen Hescheler, Thorsten Wahlers, Yeong-Hoon Choi

“…Choosing this route for the establishment of a sustainable, autologous source of cells for cardiac therapies holds the potential of being clinically more acceptable than transgenic manipulation of cells.…”
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Development of the membrane ceiling method for in vitro spermatogenesis by Maki Kamoshita, Hiroki Shirai, Hiroko Nakamura, Tetsuya Kishimoto, Yuki Hatanaka, Daisuke Mashiko, Katsuhiro Esashika, Jingjing Yang, Satoshi Yamasaki, Takehiko Ogawa, Hiroshi Kimura, Masahito Ikawa

“…Weekly time-lapse live imaging enabled us to observe transgenically fluorescent acrosome and nuclear shape formation throughout spermatogenesis. …”
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Isolation of Salt Stress-Related Genes from Aspergillus glaucus CCHA by Random Overexpression in Escherichia coli by Jie Fang, Xiaojiao Han, Lihua Xie, Mingying Liu, Guirong Qiao, Jing Jiang, Renying Zhuo

“…Five putative positive clones were obtained from E. coli cells grown on LB agar containing 1 M NaCl, on which they showed rapid growth compared to the empty vector control line. Analysis of transgenic Arabidopsis thaliana lines overexpressing CCHA-2142 demonstrated that the gene conferred increased salt tolerance to plants as well by protecting the cellular membranes, suppressing the inhibition of chlorophyll biosynthesis. …”
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Amyloid β Enhances Typical Rodent Behavior While It Impairs Contextual Memory Consolidation by Karla Salgado-Puga, Roberto A. Prado-Alcalá, Fernando Peña-Ortega

“…Despite the fact that AD transgenic mice show some evidence of reduced burrowing, it has not been yet determined whether or not Aβ can affect this typical rodent behavior and whether this alteration correlates with the well-known Aβ-induced memory impairment. …”
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Temporal Requirements of cMyc Protein for Reprogramming Mouse Fibroblasts by Corey Heffernan, Huseyin Sumer, Luis F. Malaver-Ortega, Paul J. Verma

“…Thus, the feasibility of substituting cMyc transgene with (non-integrative) recombinant “pTAT-mcMyc” protein delivery was assessed, without compromising reprogramming efficiency or the pluripotent phenotype. …”
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Human APP Gene Expression Alters Active Zone Distribution and Spontaneous Neurotransmitter Release at the Drosophila Larval Neuromuscular Junction by Ekaterina A. Saburova, Alexander N. Vasiliev, Violetta V. Kravtsova, Elena V. Ryabova, Andrey L. Zefirov, Olga I. Bolshakova, Svetlana V. Sarantseva, Igor I. Krivoi

“…Experiments were performed on larval neuromuscular junctions of transgenic Drosophila melanogaster lines with different levels of human amyloid precursor protein (APP) production. …”
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Comprehensive analysis of poly(A) tails in mouse testes and ovaries using Nanopore Direct RNA Sequencing by Agnieszka Czarnocka-Cieciura, Michał Brouze, Natalia Gumińska, Seweryn Mroczek, Olga Gewartowska, Paweł S. Krawczyk, Andrzej Dziembowski

“…It consists of RNA samples isolated from wild-type and transgenic mice lacking TENT5 polymerases, which can extend poly(A) tails in the cytoplasm. …”
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Histomorphometric Evaluation of Superovulation Effect on Follicular Development after Autologous Ovarian Transplantation in Mice by Amin Tamadon, Alireza Raayat Jahromi, Farhad Rahmanifar, Mohammad Ayaseh, Omid Koohi-Hosseinabadi, Reza Moghiminasr

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Optimized rice transformation protocol for transformation of the blast susceptible Indica rice accession CO39 by Julian R. Greenwood, Anna N. Glaus

“…Conclusions This optimized transformation protocol allows for the generation and survival of healthy CO39 calli, efficient transformation of calli using Agrobacterium, and produces a high frequency of regenerated transgenic plants. These protocol modifications will be useful for optimizing the transformation and regeneration of other recalcitrant Indica rice cultivars, particularly those sensitive to antibiotics during plantlet regeneration.…”
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Role of Stat3 in Skin Carcinogenesis: Insights Gained from Relevant Mouse Models by Everardo Macias, Dharanija Rao, John DiGiovanni

“…Further studies using a transgenic mouse model with a gain of function mutant of Stat3 (Stat3C) expressed in the basal layer of the epidermis revealed a novel role for Stat3 in skin tumor progression. …”
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Continuous Delivery of D-Luciferin by Implanted Micro-osmotic Pumps Enables True Real-Time Bioluminescence Imaging of Luciferase Activity in Vivo by Shimon Gross, Ute Abraham, Julie L. Prior, Erik D. Herzog, David Piwnica-Worms

“…Circadian oscillations in the olfactory bulbs of transgenic rats expressing firefly luciferase under the control of the period1 promoter also were temporally resolved over the course of several days. …”
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Mechanism of Repeat-Associated MicroRNAs in Fragile X Syndrome by Karen Kelley, Shin-Ju E. Chang, Shi-Lung Lin

“…To solve this question, we identified the first native ramRNA in FXS and successfully developed a transgenic zebrafish model for studying its function. …”
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Transcriptomic landscape of Hras12V oncogene-induced hepatocarcinogenesis with gender disparity by Huaiyuan Di, Zhuona Rong, Nan Mao, Huiling Li, Jun Chen, Renwu Liu, Aiguo Wang

“…A transcriptomic database was established via RNA-Seq of HCC (T) and the adjacent precancerous liver tissue (P) of Hras12V transgenic mice (Ras-Tg, HCC model) and the normal liver tissue of wild-type mice (W) of both sexes. …”
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Research prospects for kidney xenotransplantation: a bibliometric analysis by Shujun Yang, Mingtao Zhang, Hao Wei, Bin Zhang, Jiang Peng, Panfeng Shang, Shengkun Sun

“…High-frequency keywords illustrated the current state of development and future trends in xenotransplantation. The use of transgenic pigs and the development of coordinated co-stimulatory blockers have greatly facilitated progress in xenotransplantation research. …”
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Drosulfakinin signaling encodes early-life memory for adaptive social plasticity by Jiwon Jeong, Kujin Kwon, Terezia Klaudia Geisseova, Jongbin Lee, Taejoon Kwon, Chunghun Lim

“…Male-specific DSK signaling to cholecystokinin-like receptor 17D1 mediated the SNB plasticity. In fact, transgenic manipulations of the DSK neuron activity were sufficient to imitate the state of social experience. …”
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Cloning, expression pattern analysis, and vector construction of the BvNAC40 gene in sugar beet by FAN Ziqi, SUN Yaqing, LI Ningning, ZHANG Shaoying, LI Guolong

“…[Conclusion] The BvNAC40 gene is specifically expressed in response to drought stress in sugar beets, laying the foundation for future studies using transgenic technology.…”
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Improvements in pig agriculture through gene editing by Kristin M. Whitworth, Jonathan A. Green, Bethany K. Redel, Rodney D. Geisert, Kiho Lee, Bhanu P. Telugu, Kevin D. Wells, Randall S. Prather

“…The availability of genome editing tools that allow for the introduction of precise genetic modification at a single nucleotide resolution, while also facilitating large transgene integration in the target population, offers a solution. …”
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Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance by Maristela Ruberti, Luis Gustavo Romani Fernandes, Patricia Ucelli Simioni, Dirce Lima Gabriel, Áureo Tatsumi Yamada, Wirla Maria da Silva Cunha Tamashiro

“…In this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice. …”
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Hyperleukocytosis in a neuroblastoma patient after treatment with natural killer T cells expressing a GD2-specific chimeric antigen receptor and IL-15 by Xin Xu, Shantan Reddy, Saleh Bhar, Frederic D Bushman, Amy N Courtney, Gabriel A Barragán, Gengwen Tian, Erica J Di Pierro, Leonid S Metelitsa, Antonino Montalbano, Hangjin Yu, Claudia Martinez Amador, Nisha Ghatwai, Michael S Wood, Deborah Schady, Aoife M Roche, David de la Cerda, Donald Williams Parsons, Andras Heczey

“…These findings warrant the implementation of measures to control immune cell activation during manufacture of cell therapy products, especially those armed with transgenic cytokines.…”
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Application of a ImageJ-Based Method to Measure Blood Flow in Adult Zebrafish and Its Applications for Toxicological and Pharmacological Assessments by Ferry Saputra, Tzu-Ming Tseng, Franelyne P. Casuga, Yu-Heng Lai, Chih-Hsin Hung, Chung-Der Hsiao

“…Overall, an optimized protocol was used for the first time to measure blood flow velocity in adult wild-type zebrafish without the aid of transgenic lines or fluorescent dye.…”
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