Optimizing the thermostability of triketone dioxygenase for engineering tolerance to mesotrione herbicide in soybean and cotton by Stephen M. G. Duff, Stephen M. G. Duff, Lei Shi, Danqi Chen, Xiaoran Fu, Mingsheng Peng, Clayton T. Larue, Janice Weihe, Jessica Koczan, Brian Krebel, Qungang Qi

“…Optimized triketone dioxygenase (TDO) variants with enhanced temperature stability parameters were engineered to enable robust triketone tolerance in transgenic cotton and soybean crops. This herbicide tolerance trait, which can metabolize triketone herbicides such as mesotrione and tembotrione, could be useful for weed management systems and provide additional tools for farmers to control weeds. …”
Get full text

The SEPALLATA-like gene HrSEP1 in Hippophae rhamnoides regulates flower development by interacting with other MADS-box subfamily genes by Di Cong, Xue Zhao, Xue Zhao, Chang Ni, Mengru Li, Luwen Han, Jianlin Cheng, Hongzhang Liu, Huijing Liu, Dan Yao, Shuying Liu, Guoshuang Chen

“…We also found a strong synergy between HrSEP1 and AP1 genes in sepals, petals, and stamens by transgenic methods and fluorescence quantitative PCR, suggesting that HrSEP1 and AP1 may co-regulate the development of these structures. …”
Get full text

The AβA2V paradigm: From molecular insights to therapeutic strategies in Alzheimer’s disease and primary tauopathies by Luisa Diomede, Andrea Conz, Michele Mosconi, Tatiana Stoilova, Matteo Paloni, Matteo Salvalaglio, Alfredo Cagnotto, Laura Colombo, Marcella Catania, Giuseppe Di Fede, Fabrizio Tagliavini, Mario Salmona

“…The therapeutic potential of this peptide emerges from its ability to reduce amyloid β-induced toxicity, with promising results from studies in human neuroblastoma cells and transgenic animal models.…”
Get full text

Overexpression of a sorghum SnRK1βγ2 gene increases the biomass in Setaria viridis but not in rice by Chudamani Sharma Prakash, Lihua Wang, Qi Shen, Jieqin Li, Yi-Hong Wang

“…In addition, SbSnRK1βγ2 did not affect the panicle weight in sorghum, but its overexpression doubled the panicle weight in S. viridis in all four evaluated transgenic lines. Overall, the overexpression of SbSnRK1βγ2 tripled the biomass production in S. viridis, indicating SbSnRK1βγ2's potential in any future cellulosic biofuel production and S. viridis' utility as an alternative genetic vehicle to functionally characterise sorghum genes.…”
Get full text

Igf2/H19 Imprinting Control Region (ICR): An Insulator or a Position-Dependent Silencer? by Subhasis Banerjee, Alan Smallwood, Scott Lamond, Stuart Campbell, Geeta Nargund

“…Furthermore, a localised folded chromatin structure resulting from juxtaposition of two disparate regulatory sequences (enhancer ICR) could be the mechanistic basis of ICR-mediated position-dependent (ICR-promoter) transcriptional repression in transgenic Drosophila.…”
Get full text

The hypertrophic cardiomyopathy-associated A331P actin variant enhances basal contractile activity and elicits resting muscle dysfunction by Matthew H. Doran, Michael J. Rynkiewicz, Evan Despond, Meera C. Viswanathan, Aditi Madan, Kripa Chitre, Axel J. Fenwick, Duncan Sousa, William Lehman, John F. Dawson, Anthony Cammarato

“…To help resolve disease pathogenesis, we implemented a multidisciplinary approach. Transgenic Drosophila, expressing A331P actin, displayed skeletal muscle hypercontraction and elevated basal myocardial activity. …”
Get full text

Whole organism and tissue-specific analysis of pexophagy in Drosophila by Francesco G. Barone, Marco Marcello, Sylvie Urbé, Natalia Sanchez-Soriano, Michael J. Clague

“…Peroxisomes are essential organelles involved in critical metabolic processes in animals such as fatty acid oxidation, ether phospholipid production and reactive oxygen species detoxification. We have generated transgenic Drosophila melanogaster models expressing fluorescent reporters for the selective autophagy of peroxisomes, a process known as pexophagy. …”
Get full text

Identification of Two Novel HLA-A∗0201-Restricted CTL Epitopes Derived from MAGE-A4 by Zheng-Cai Jia, Bing Ni, Ze-Min Huang, Yi Tian, Jun Tang, Jing-Xue Wang, Xiao-Lan Fu, Yu-Zhang Wu

“…Of selected four peptides (designated P1, P2, P3, and P4), P1 (MAGE-A4286-294, KVLEHVVRV) and P3 (MAGE-A4272-280, FLWGPRALA) could elicit peptide-specific CTLs both in vitro from HLA-A∗0201-positive PBMCs and in HLA-A∗0201/Kb transgenic mice. And the induced CTLs could lyse target cells in an HLA-A∗0201-restricted fashion, demonstrating that the two peptides are HLA-A∗0201-restricted CTL epitopes and could serve as targets for therapeutic antitumoral vaccination.…”
Get full text

The Peroxisomal 3-keto-acyl-CoA thiolase B Gene Expression Is under the Dual Control of PPARα and HNF4α in the Liver by J. Chamouton, F. Hansmannel, J. A. Bonzo, M. C. Clémencet, G. Chevillard, M. Battle, P. Martin, T. Pineau, S. Duncan, F. J. Gonzalez, N. Latruffe, S. Mandard, V. Nicolas-Francès

“…PPARα and HNF4α are nuclear receptors that control gene transcription by direct binding to specific nucleotide sequences. Using transgenic mice deficient for either PPARα or HNF4α, we show that the expression of the peroxisomal 3-keto-acyl-CoA thiolase B (Thb) is under the dependence of these two transcription factors. …”
Get full text

Intranasally administrated fusion-inhibitory lipopeptides block SARS-CoV-2 infection in mice and enable long-term protective immunity by Said Mougari, Valérie Favède, Camilla Predella, Olivier Reynard, Stephanie Durand, Magalie Mazelier, Edoardo Pizzioli, Didier Decimo, Francesca T. Bovier, Lauren M. Lapsley, Candace Castagna, Nicole A. P. Lieberman, Guillaume Noel, Cyrille Mathieu, Bernard Malissen, Thomas Briese, Alexander L. Greninger, Christopher A. Alabi, N. Valerio Dorrello, Stéphane Marot, Anne-Geneviève Marcelin, Ana Zarubica, Anne Moscona, Matteo Porotto, Branka Horvat

“…Abstract We have assessed antiviral activity and induction of protective immunity of fusion-inhibitory lipopeptides derived from the C-terminal heptad-repeat domain of SARS-CoV-2 spike glycoprotein in transgenic mice expressing human ACE2 (K18-hACE2). The lipopeptides block SARS-CoV-2 infection in cell lines and lung-derived organotypic cultures. …”
Get full text

Pseudomonas syringae lytic transglycosylase HrpH interacts with host ubiquitin ligase ATL2 to modulate plant immunity by Jun-Zhou Li, Yi-Lin Gu, Wei Zhang, Shen Cong, Ruo-Na Wang, Yi-Nan Ma, Ya Jin, Hai-Lei Wei

“…HrpH and its SLT domain induce host cell death and suppress pattern-triggered immunity (PTI). Transgenic hrpH-Arabidopsis plants exhibit decreased PTI responses and enhanced susceptibility to Pst DC3000ΔhrcQ-U. …”
Get full text

Fecal Microbiota Transplantation from Methionine-Restricted Diet Mouse Donors Improves Alzheimer’s Learning and Memory Abilities Through Short-Chain Fatty Acids by Run Yu, Haimeng Zhang, Rui Chen, Yangzhuo Lin, Jingxuan Xu, Ziyang Fang, Yuehang Ru, Chenhan Fan, Guoqing Wu

“…In this study, APP/PS1 double transgenic AD mice were used and an FMT experiment was conducted. 16S rRNA gene sequencing, targeted metabolomics, and microbial metabolite short-chain fatty acids (SCFAs) of feces samples were analyzed. …”
Get full text

Two Small Peptides from <i>Buthus martensii</i> Hydrolysates Exhibit Antitumor Activity Through Inhibition of TNF-α-Mediated Signal Transduction Pathways by Mengshuang Zhu, Shanshan Zhang, Jiyang Tang, Hairong Hou, Lizhen Wang, Houwen Lin, Xuanming Zhang, Meng Jin

“…In silico prediction of therapeutic targets, MGC-803 cells and transgenic zebrafish models, and immunoblotting experiments were used to reveal the molecular mechanism of action of the peptides. …”
Get full text

CREB Regulates Experience-Dependent Spine Formation and Enlargement in Mouse Barrel Cortex by Annabella Pignataro, Antonella Borreca, Martine Ammassari-Teule, Silvia Middei

“…Here we studied the effects of vibrissae trimming, a widely used model of sensory deprivation-induced cortical plasticity, on processes associated with dendritic spine rearrangements in the barrel cortex of a transgenic mouse model of CREB downregulation (mCREB mice). …”
Get full text

Optimizing a Rodent Model of Parkinson's Disease for Exploring the Effects and Mechanisms of Deep Brain Stimulation by Karl Nowak, Eilhard Mix, Jan Gimsa, Ulf Strauss, Kiran Kumar Sriperumbudur, Reiner Benecke, Ulrike Gimsa

“…An outlook is given on further developments of experimental DBS, including the use of transgenic animals and the testing of closed-loop systems for the direct on-demand application of electric stimulation.…”
Get full text

A high-fidelity CRISPR-Cas13 system improves abnormalities associated with C9ORF72-linked ALS/FTD by Tristan X. McCallister, Colin K. W. Lim, Mayuri Singh, Sijia Zhang, Najah S. Ahsan, William M. Terpstra, Alisha Y. Xiong, M. Alejandra Zeballos C, Jackson E. Powell, Jenny Drnevich, Yifei Kang, Thomas Gaj

“…When delivered to the brain of a transgenic rodent model, this Cas13-based platform curbed the expression of the G4C2 repeat-containing RNA without affecting normal C9ORF72 levels, which in turn decreased the formation of RNA foci, reduced the production of a dipeptide repeat protein, and reversed transcriptional deficits. …”
Get full text

Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse by Lisi Deng, Guangze Liu, Xin Wu, Yaping Wang, Minghua Tong, Bing Liu, Kuidong Wang, Yuting Peng, Xiangping Kong

“…ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. …”
Get full text

Local Gene Transfer of OPG Prevents Joint Damage and Disease Progression in Collagen-Induced Arthritis by Qingguo Zhang, Weiming Gong, Bin Ning, Lin Nie, Paul H. Wooley, Shang-You Yang

“…A single periarticular injection of AAV-OPG or AAV-LacZ on the arthritic paw successfully incorporated the exogenous gene to the local tissue and resulted in marked transgene expression in the joint homogenate for at least three weeks. …”
Get full text

Porcine transient receptor potential channel 1 promotes adipogenesis and lipid deposition by Yu Fu, Xin Hao, Jingru Nie, Peng Shang, Xinxing Dong, Bo Zhang, Dawei Yan, Hao Zhang

“…Functional analysis showed that TRPC1 promotes primary preadipocyte proliferation and adipogenic differentiation. In vivo, transgenic mice expressing porcine TRPC1 exhibited aggravated high-fat diet–induced obesity, hepatic steatosis, and insulin resistance. …”
Get full text

Coexistence of Gait Disturbances and Chorea in Experimental Huntington’s Disease by João Casaca-Carreira, Yasin Temel, Marloes van Zelst, Ali Jahanshahi

“…In this study, we aimed to investigate and characterize the gait abnormalities and choreic movements in a transgenic rat model of HD (tgHD). TgHD presents typical neuropathological, neurophysiological, and behavioral aspects mimicking some of the key features of human HD and is the only described experimental model for HD that exhibits choreiform movements. …”
Get full text