Mouse Reporter Strain for Noninvasive Bioluminescent Imaging of Cells that have Undergone Cre-Mediated Recombination by Michal Safran, William Y. Kim, Andrew L. Kung, James W. Horner, Ron A. DePinho, William G. Kaelin

“…ROSA26 L-S-L-Luc /+ mice expressed luciferase in a diffuse or liver-restricted pattern, as determined by noninvasive, bioluminescent imaging, when crossed to transgenic mice in which Cre was under the control of a zygotically expressed (EIIA-Cre), or a liver-restricted (albumin-Cre), promoter, respectively. …”
Get full text

The interaction of tPA with NMDAR1 drives neuroinflammation and neurodegeneration in α-synuclein-mediated neurotoxicity by Daniel Torrente, Enming J. Su, Alí Francisco Citalán-Madrid, Gerald P. Schielke, Daniel Magaoay, Mark Warnock, Tamara Stevenson, Kris Mann, Flavie Lesept, Nathalie Delétage, Manuel Blanc, Erin H. Norris, Denis Vivien, Daniel A. Lawrence

“…To characterize the mechanism of tPA action in α-synuclein-mediated pathology in the SN and to identify possible therapeutic pathways, we performed RNA-seq analysis of the SN and used multiple transgenic mouse models. These included tPA deficient mice and two newly developed transgenic mice, a knock-in model expressing endogenous levels of proteolytically inactive tPA (tPA Ala-KI) and a second model overexpressing proteolytically inactive tPA (tPA Ala-BAC). …”
Get full text

Lingguizhugan decoction enhances autophagy of Alzheimer’s disease via regulating the mTOR/ p70s6K pathway in vivo and in vitro by Xiaojing Chen, Qingrong Tian, Min Gao, Xibin Zhou, Chunxiang Zhou

“…IntroductionLingguizhugan decoction (LGZG) has been reported to treat Alzheimer’s disease (AD) by anti-inflammatory and transporting amyloid-β (Aβ).MethodsUsing APP/PS1 transgenic mice as in vivo model and gave LGZG decoction by oral gavage. …”
Get full text

Glutamatergic CYLD deletion leads to aberrant excitatory activity in the basolateral amygdala: association with enhanced cued fear expression by Huidong Li, Faqin Li, Zhaoyi Chen, Erwen Wu, Xiaoxi Dai, Danni Li, Haojie An, Shiyi Zeng, Chunyan Wang, Li Yang, Cheng Long

“…We next constructed transgenic model mice with specific deletion of Cyld from glutamatergic neurons. …”
Get full text

Intein-mediated split SaCas9 for genome editing in plants by Danling Hu, Lizhe Hu, Yaqiang Lu, Xiao Dong, Xingyu Cao, Shasha Bai, Lingang Zhang, Dongming Li, Yongwei Sun

“…The Split-v1 system demonstrated genome editing efficiencies in transgenic plants comparable to those achieved with wild-type SaCas9, with efficiencies ranging from 70.2% to 96.1%. …”
Get full text

Electroconvulsive shock and transcranial magnetic stimulation do not alter the survival or spine density of adult-born striatal neurons. by Tara Gaertner, Tian Rui Zhang, Baran Askari, Fidel Vila-Rodriguez, Jason S Snyder

“…Adult-born cells were labelled in transgenic mice and 8 days later mice were given 10 stimulations over the course of 3 weeks. …”
Get full text

Three-Dimensional in Vivo Imaging of Green Fluorescent Protein-Expressing T Cells in Mice with Noncontact Fluorescence Molecular Tomography by Anikitos Garofalakis, Giannis Zacharakis, Heiko Meyer, Eleftherios N. Economou, Clio Mamalaki, Joseph Papamatheakis, Dimitris Kioussis, Vasilis Ntziachristos, Jorge Ripoll

“…In this study, the system was applied to determining the distribution of green fluorescent protein (GFP)-expressing T lymphocytes in a transgenic mouse model, thus demonstrating the potential of the system for studying immune system function. …”
Get full text

Circuitry and Synaptic Dysfunction in Alzheimer’s Disease: A New Tau Hypothesis by Siddhartha Mondragón-Rodríguez, Humberto Salgado-Burgos, Fernando Peña-Ortega

“…Further supporting this alternative role of pTau, we have recently shown that early increases in pTau close to MD sites prevent hippocampal circuit overexcitation in a transgenic AD mouse model. Here, we will synthesize this new evidence that confronts the leading Tau-based AD hypothesis and discuss the role of pTau modulating neural circuits and network connectivity. …”
Get full text

Clinical Trials of Immunogene Therapy for Spontaneous Tumors in Companion Animals by Gerardo Claudio Glikin, Liliana María Elena Finocchiaro

“…In some cases autologous, allogenic, or xenogeneic transgenic cytokine producing cells were assayed. In general terms, minor or no adverse collateral effects appeared during this kind of therapies and treated patients usually displayed a better course of the disease (longer survival, delayed or suppressed recurrence or metastatic spread, and improvement of the quality of life). …”
Get full text

Noninvasive Monitoring of mRFP1- and mCherry-Labeled Oncolytic Adenoviruses in an Orthotopic Breast Cancer Model by Spectral Imaging by Anton V. Borovjagin, Lacey R. McNally, Minghui Wang, David T. Curiel, Mary J. MacDougall, Kurt R. Zinn

“…Capsid labeling in conjunction with spectral imaging thus enables direct visualization and quantification of CRAd particles in tumors prior to the reporter transgene expression. This allows for noninvasive control of CRAd delivery and distribution in tumors and facilitates quantitative assessment of viral replication. …”
Get full text

The Role of NK Cells in Pig-to-Human Xenotransplantation by Gisella Puga Yung, Mårten K. J. Schneider, Jörg D. Seebach

“…The potential of cell-based strategies including tolerogenic dendritic cells (DC) and regulatory T cells (Treg) and the latest progress using transgenic pigs genetically modified to reduce xenogeneic NK cell responses are discussed. …”
Get full text

Targeting Herpetic Keratitis by Gene Therapy by Hossein Mostafa Elbadawy, Marine Gailledrat, Carole Desseaux, Diego Ponzin, Stefano Ferrari

“…Whereas the correction of inherited diseases was the initial goal of the field of gene therapy, here we discuss transgene expression, gene replacement, silencing, or clipping. …”
Get full text

Inhibiting C-Reactive Protein for the Treatment of Cardiovascular Disease: Promising Evidence from Rodent Models by Alexander J. Szalai, Mark A. McCrory, Dongqi Xing, Fadi G. Hage, Andrew Miller, Suzanne Oparil, Yiu-Fai Chen, Michelle Mazzone, Richard Early, Scott P. Henry, Thomas A. Zanardi, Mark J. Graham, Rosanne M. Crooke

“…Likewise in human CRP transgenic mice treated for 2 weeks with a human CRP-specific ASO, blood human CRP was reduced by >70% and carotid artery patency was improved (2 weeks after surgical ligation). …”
Get full text

Wolfberry extracts inhibit Aβ1-42 aggregation and rescue memory loss of AD drosophila by Cai-Hong Li, Jun-Li Yang

“…Total extracts of all wolfberry samples showed the potent to decrease self-induced Aβ1-42 aggregation and Cu2+-induced Aβ1-42 aggregation and the antioxidant capacity as well as the bioactivity for binding bio-metal. A transgenic drosophila model assay revealed that TE of S1 and S6 (S1-TE and S6-TE) at 100 μg/mL demonstrated the potent rescue effect on memory loss compared to the positive control memantine at 20 μg/mL. …”
Get full text

Amyloid-Beta Induced Changes in Vesicular Transport of BDNF in Hippocampal Neurons by Bianca Seifert, Robert Eckenstaler, Raik Rönicke, Julia Leschik, Beat Lutz, Klaus Reymann, Volkmar Lessmann, Tanja Brigadski

“…Taken together, extracellular cleavage products of APP induced rapid changes in anterograde and retrograde transport of BDNF-containing vesicles while release of BDNF was unaffected by transgenic expression of mutated APP. These early transport deficits might lead to permanently impaired brain functions in the adult brain.…”
Get full text

Delineating Molecular Mechanisms of Squamous Tissue Homeostasis and Neoplasia: Focus on p63 by Kathryn E. King, Linan Ha, Tura Camilli, Wendy C. Weinberg

“…In vitro keratinocytes and murine transgenic and transplantation models have been invaluable in elucidating the contribution of altered p63 levels to cancer development, and studies have identified the roles for ΔNp63 isoforms in keratinocyte survival and malignant progression, likely due in part to their transcriptional regulatory function. …”
Get full text

Identification of U6 Promoter and Establishment of Gene-Editing System in <i>Larix kaempferi</i> (Lamb.) Carr by Jun-Xia Xing, Ao-Jie Luo, Xin-Hao Wang, Qi Ding, Ling Yang, Wan-Feng Li

“…The results showed various mutations in the transgenic materials, including base substitutions and deletions, and the editing frequency ranged from 5% to 14.29%. …”
Get full text

Rapid lightsheet fluorescence imaging of whole Drosophila brains at nanoscale resolution by potassium acrylate-based expansion microscopy by Xuejiao Tian, Tzu-Yang Lin, Po-Ting Lin, Min-Ju Tsai, Hsin Chen, Wen-Jie Chen, Chia-Ming Lee, Chiao-Hui Tu, Jui-Cheng Hsu, Tung-Han Hsieh, Yi-Chung Tung, Chien-Kai Wang, Suewei Lin, Li-An Chu, Fan-Gang Tseng, Yi-Ping Hsueh, Chi-Hon Lee, Peilin Chen, Bi-Chang Chen

“…Abstract Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixation in the nervous system. …”
Get full text

Unveiling sequence-agnostic mixed-chemical modification patterns for splice-switching oligonucleotides using the NATURA platform by Tommaso Tabaglio, Taniya Agarwal, Wei Yuan Cher, Jin Rong Ow, Ah Keng Chew, Priscila Yun Qian Sun, Raja Sekhar Reddy Gurrampati, Hongfang Lu, Praveena Naidu, Hong Kai Ng, Xavier Le Guezennec, Shi Yan Ng, Manikandan Lakshmanan, Ernesto Guccione, Keng Boon Wee

“…NATURA uses a reporter gene system and a comprehensive sequence library to test modifications and delivery methods, validated in a transgenic mouse model. This approach aims to accelerate NAT development and address challenges in delivering these therapies to patients.…”
Get full text

The Evolution of Immunosuppressive Therapy in Pig-to-Nonhuman Primate Organ Transplantation by S. A. Sanatkar, K. Kinoshita, A. Maenaka, H. Hara, D. K. C. Cooper

“…Major steps were the identification and deletion of expression of the three known glycan xenoantigens on pig vascular endothelial cells, the transgenic expression of human “protective” proteins, e.g., complement-regulatory, coagulation-regulatory, and anti-inflammatory proteins, and the administration of an immunosuppressive regimen based on blockade of the CD40/CD154 T cell co-stimulation pathway. …”
Get full text