A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. by Haania Khan, Xinyu Huang, Vishnu Raj, Han Wang

“…The ability to manipulate gene activity and control transgene expression is essential to study gene function. …”
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Use of a Sendai virus-based vector for effcient transduction of pinniped fbroblasts by V. R. Beklemisheva, A. G. Menzorov

“…Also, transgene expression after Sendai virus­based transduction is quite stable and changes only slightly at day four compared to day two. …”
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Construction of oncolytic reporter influenza viral vectors and assessment of their safety following intracranial administration in rats by A. A. Pulkina, A. S. Mustafaeva, E. A. Romanovskaya-Romanko, M. A. Plotnikova, O. O. Ozhereleva, M. A. Shuklina, L. N. Kiseleva, Y. S. Alekseeva, N. S. Kurmazov, A. R. Muslimov, A.-P.S. Shurygina, M. A. Stukova

“…However, vectors containing the 2A site upstream of the transgene infected the glioma cell lines C6, A172, and T98G more effectively. …”
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Activation of Wnt/β-catenin in neural progenitor cells regulates blood–brain barrier development and promotes neuroinflammation by Dylan J. Sebo, Irshad Ali, Audrey R. Fetsko, Aubrey A. Trimbach, Michael R. Taylor

“…To further test this concept, we generated a transgenic Tet-On inducible system to drive constitutively active β-catenin expression in neural progenitor cells (NPCs). …”
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PU.1 and Haematopoietic Cell Fate: Dosage Matters by Ka Sin Mak, Alister P. W. Funnell, Richard C. M. Pearson, Merlin Crossley

“…This paper will discuss how a range of experiments in cell lines and mutant and transgenic mouse models have enhanced our knowledge of the mechanisms by which PU.1 drives lineage-specific differentiation during haematopoiesis.…”
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Regulation of Murine Ovarian Epithelial Carcinoma by Vaccination against the Cytoplasmic Domain of Anti-Müllerian Hormone Receptor II by Cagri Sakalar, Suparna Mazumder, Justin M. Johnson, Cengiz Z. Altuntas, Ritika Jaini, Robert Aguilar, Sathyamangla V. Naga Prasad, Denise C. Connolly, Vincent K. Tuohy

“…In addition, prophylactic AMHR2-CD vaccination of TgMISIIR-TAg transgenic mice significantly inhibited growth of autochthonous EOCs and provided a 41.7% increase in mean overall survival. …”
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Functional study on the regulation of adventitious root formation by PpSAT4 gene in peach rootstock ‘GF 677’ by HAO Lanlan, WANG Hong, ZHANG Fan, ZHANG Xuebing, YANG Yang

“…Analysis of cis-acting elements showed the presence of response elements such as light, auxin, and gibberellin in the PpSAT4 sequence. (2) The overexpression vector Pp- SAT4-CAM-FLAG was constructed and transformed P . domestica and A . thaliana, and the transgenic lines were obtained. (3) qRT-PCR analysis showed that the expression level of PpSAT4 in the transgenic lines was higher than that of the wild-type. …”
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Identification and Characterization of the Diverse Stress-Responsive R2R3-RMYB Transcription Factor from Hibiscus sabdariffa L. by Bahaeldeen Babikar Mohamed, Beenish Aftab, Muhammad Bilal Sarwar, Bushra Rashid, Zarnab Ahmad, Sameera Hassan, Tayyab Husnain

“…Overexpression of RMYB was observed in transgenic plants under abiotic stresses which further suggests its regulatory role in response to stressful conditions. …”
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Stratégies paysannes pour la protection du maïs indigène dans l'État de Tlaxcala by Pánfilo Hernández Ortiz

“…The article presents the strategies deployed by the Vicente Guerrero Group in Tlaxcala to prevent the use of transgenic corn in the region, as well as to revitalize the use of native varieties of corn. …”
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Immunopathology of Airway Surface Liquid Dehydration Disease by Brandon W. Lewis, Sonika Patial, Yogesh Saini

“…As an alternative, the Scnn1b transgenic (Scnn1b-Tg+) mouse model overexpressing a transgene encoding sodium channel nonvoltage-gated 1, beta subunit (Scnn1b) in airway club cells is available. …”
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Cell Surface Proteins for Enrichment and In Vitro Characterization of Human Pluripotent Stem Cell-Derived Myogenic Progenitors by Sin-Ruow Tey, Madison Mueller, Megan Reilly, Colton Switalski, Samantha Robertson, Mariko Sakanaka-Yokoyama, Masatoshi Suzuki

“…We previously reported a transgene-free, serum-free floating sphere culture method for the derivation of myogenic progenitors from human PSCs. …”
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Тransplastomic tobacco plants producing the hydrophilic domain of the sheep pox virus coat protein L1R by D. K. Beisenov, G. E. Stanbekova, B. K. Iskakov

“…It provides integration of the transgene into the trnG/trnfM region of the chloroplast tobacco genome by homologous recombination. …”
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TGFβ1 Controls PPARγ Expression, Transcriptional Potential, and Activity, in Part, through Smad3 Signaling in Murine Lung Fibroblasts by Allan Ramirez, Erin N. Ballard, Jesse Roman

“…In these cells, stimulation of TGFβ1 signals with a constitutively active TGFβ1 receptor transgene blunted PPARγ-dependent reporter expression induced by troglitazone, a PPARγ activator. …”
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Cloning and expression characterisation of LiDXS2 gene in Oriental Lily (Lilium 'Sorbonne') by Yu Jiaxuan, Wu Nana, Fu Ruyu, Xue Lili, Zhang Jinzhu, Dong Jie, Yang Tao, Fan Jinping

“…The plant expression vector was constructed to transform Arabidopsis thaliana, and the transgenic plants were characterised. The results showed that the LiDXS2 ORF area was 2142 bp and encoded a total of 713 amino acids. …”
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Selective epithelial expression of KRASG12D in the Oncopig pancreas drives ductal proliferation and desmoplasia that is accompanied by an immune response by Carlos P. Jara, Al-Murtadha Al-Gahmi, Audrey Lazenby, Michael A. Hollingsworth, Mark A. Carlson

“…Intraductal pancreatic injections in transgenic Oncopigs (LSL-KRAS G12D-TP53 R167H) were performed with histologically based evaluation at 2 months post-injection. …”
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Exercise-Intervened Circulating Extracellular Vesicles Alleviate Oxidative Stress in Cerebral Microvascular Endothelial Cells Under Hypertensive Plus Hypoxic Conditions by Smara Sigdel, Shuzhen Chen, Gideon Udoh, Jinju Wang

“…In this study, we investigated the influence of exercise on cEV level and function. Transgenic hypertensive mice (Alb1-Ren) underwent 8-week treadmill exercise (10 m/min for 1 h, 5 days per week). …”
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Phytoalexin deficient 4 is associated with the lesion mimic trait in watermelon clalm mutant (Citrullus lanatus) by Jiale Shi, Congji Yang, Yuanyuan Qin, Qingqing Liu, Shengqi Hua, Defeng Wu, Wei Dong

“…RNA interference of the ClPAD4 yielded transgenic lines whose cotyledon prone to diseased over 2 weeks. …”
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A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy by N. P. Malakhova, Y. A. Skiba, G. A. Iskakova, D. A. Naizabayeva, B. K. Tezekbaeva, G. A. Ismagulova, E. R. Maltseva

“…The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. For different plant objects, the efficiency of obtaining transgenic plants by the ballistic method varies from 1 to 3 %. …”
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Effect of custom-designed transfection buffer on delivery of genome modification components into primary cells of buffalo, cattle, goats, and sheep by Shreya Malhotra, Priyanka Singh, Aseem Tara, Bosco Jose, Devika Gautam, Ram Prasad, Gaurav Tripathi, Sacchinandan De, Naresh L. Selokar

“…In conclusion, our lab-made transfection buffer can be efficiently used to generate genome-edited or transgenic farm animals for agriculture, biomedical, and veterinary applications. …”
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Quantification and correlation of amyloid-β plaque load, glial activation, GABAergic interneuron numbers, and cognitive decline in the young TgF344-AD rat model of Alzheimer’s dise... by Anett Futácsi, Anett Futácsi, Anett Futácsi, Kitti Rusznák, Kitti Rusznák, Gergely Szarka, Gergely Szarka, Gergely Szarka, Béla Völgyi, Béla Völgyi, Ove Wiborg, Boldizsár Czéh, Boldizsár Czéh, Boldizsár Czéh

“…Cholecystokinin-positive cell numbers were decreased in the hippocampus of transgenic rats, but calretinin-, parvalbumin-, and somatostatin-positive cell numbers were not altered. …”
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