Bioluminescence Imaging of Vascular Endothelial Growth Factor Promoter Activity in Murine Mammary Tumorigenesis by Shannon L. Faley, Keiko Takahashi, Cornelia E. Crooke, Joshua T. Beckham, Takuya Tomemori, Scott B. Shappell, E. Duco Jansen, Takamune Takahashi

“…Vascular endothelial growth factor (VEGF) is a major inducer of angiogenesis. We generated a transgenic reporter mouse, VEGF-GL, in which an enhanced green fluorescent protein-luciferase fusion protein is expressed under the control of a human VEGF-A promoter. …”
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Live Visualization of Calcified Bones in Zebrafish and Medaka Larvae and Juveniles Using Calcein and Alizarin Red S by Rina Koita, Sae Oikawa, Taisei Tani, Masaru Matsuda, Akinori Kawamura

“…A prevalent technique for investigating calcified bone development in these species involves using transgenic fish that express fluorescent proteins labeling calcified bones; however, acquiring these fish and isolating appropriate crosses can be time-consuming. …”
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Overexpression of CsBRC, an F‐box gene from Camellia sinensis, increased the plant branching in tobacco and rice by Bokun Zhou, Qi Sheng, Xinzhuan Yao, Tong Li, Litang Lu

“…By comparing the phenotypes of CsBRC transgenic tobacco and WT tobacco, it was found that the number of branches of transgenic tobacco was significantly higher than that of wild‐type tobacco. …”
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Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions by Chad C. MacArthur, Andrew Fontes, Namritha Ravinder, David Kuninger, Jasmeet Kaur, Matthew Bailey, Antje Taliana, Mohan C. Vemuri, Pauline T. Lieu

“…Here, we report the generation of iPSCs by an RNA Sendai virus vector that does not integrate into the cells genome, providing transgene-free iPSC line. In addition, reprogramming can be performed in feeder-free condition with StemPro hESC SFM medium and in xeno-free (XF) conditions. …”
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Group a Streptococcus remains viable inside fibrin clots and gains access to human plasminogen for subsequent fibrinolysis and dissemination by Henry M. Vu, Thomas E. Moran, Zhong Liang, Yun-Juan Bao, Paulina G. Carles, Jessica C. Keane, Madelyn G. Cerney, Caitlyn N. Dahnke, Ana L. Flores-Mireles, Victoria A. Ploplis, Francis J. Castellino, Shaun W. Lee

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Model System for Live Imaging of Neuronal Responses to Injury and Repair by Mathieu Gravel, Yuan-Cheng Weng, Jasna Kriz

“…Reporter functionality was first observed during the development of transgenic embryos. Using in vivo bioluminescence and fluorescence imaging, we visualized induction of the GAP-43 signals from live embryos starting at E10.5, as well as neuronal responses to brain and peripheral nerve injuries (the signals peaked at 14 days postinjury). …”
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Spines, Plasticity, and Cognition in Alzheimer's Model Mice by Tara Spires-Jones, Shira Knafo

“…Here, we review work on transgenic AD models that investigate the degeneration of dendritic spine structure, synaptic function, and cognition. …”
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Reprogramming of Mouse Calvarial Osteoblasts into Induced Pluripotent Stem Cells by Yinxiang Wang, Jessica Aijia Liu, Keith K. H. Leung, Mai Har Sham, Danny Chan, Kathryn S. E. Cheah, Martin Cheung

“…Here we adopted fluorescence-activated cell sorting-based strategy to isolate homogenous population of intramembranous calvarial osteoblasts from newborn transgenic mice carrying both Osx1-GFP::Cre and Oct4-EGFP transgenes. …”
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GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) by Mingliang Yang, Mingliang Yang, Chengjun Lei, Chengjun Lei, Chao Ma, Chao Ma, Xiuming Hou, Xiuming Hou, Mingming Yao, Mingming Yao, Liang Mi, Liang Mi, Enliang Liu, Linli Xu, Shukun Wang, Shukun Wang, Chunyan Liu, Chunyan Liu, Qingshan Chen, Qingshan Chen, Dawei Xin, Dawei Xin, Chang Xu, Chang Xu, Jinhui Wang, Jinhui Wang

“…WGCNA analyses led to the identification of GmWRKY33a as being responsive to BR signaling in the context of symbiosis establishment. Transgenic hairy roots analyses indicated that GmWRKY33a served as a negative regulator of the establishment of symbiosis. …”
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Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance by Ming Luo, Sitao Zhu, Hua Dang, Qing Wen, Ruixia Niu, Jiawei Long, Zhao Wang, Yongjia Tong, Yuese Ning, Meng Yuan, Guoyong Xu

“…Unlike prevailing targeted protein degradation strategies, the TCD system does not rely on small molecules, antibodies, or genetic knock-in fusion tags, demonstrating its promise as a transgene-based approach for optimizing crop performance.…”
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Challenges in developing a split drive targeting dsx for the genetic control of the invasive malaria vector Anopheles stephensi by Mireia Larrosa-Godall, Joshua X. D. Ang, Philip T. Leftwich, Estela Gonzalez, Lewis Shackleford, Katherine Nevard, Rob Noad, Michelle A. E. Anderson, Luke Alphey

“…Separately a transgenic line which expresses a gRNA targeting the female specific exon of dsx was inserted into that same target site. …”
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Combined mutations in nonstructural protein 14, envelope, and membrane proteins mitigate the neuropathogenicity of SARS-CoV-2 Omicron BA.1 in K18-hACE2 mice by Kotou Sangare, Shufeng Liu, Prabhuanand Selvaraj, Charles B. Stauft, Matthew F. Starost, Tony T. Wang

“…ABSTRACT We previously reported that mutations outside the spike protein play a role in the attenuation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron BA.1 variant in human ACE2 transgenic mice (K18-hACE2). Here, we assessed the pathogenicity of SARS-CoV-2 (WA1/2020) containing mutations from the Omicron BA.1 variant in K18-hACE2 mice. …”
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Genetic Transformability of Selected Kenyan Groundnut (Arachis hypogaea L.) Genotypes with IPT Gene Using Cotyledonary Node Explants by Purity G. Limbua, Mathew P. Ngugi, Richard O. Oduor

“…Putative transformants were tested for the presence of the transgene by PCR designed to amplify the IPT gene sequence. …”
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Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals by Jinhui Cui, Haixin Cui, Yan Wang, Changjiao Sun, Kui Li, Hongyan Ren, Wei Du

“…To evaluate the performance of the magnetic nanoparticles as gene transfer vector for breeding transgenic animals, we investigated a new approach to deliver green fluorescent protein (GFP) gene to porcine kidney 15 (PK-15) and porcine embryonic fibroblast (PEF) cells using PEI-modified magnetic nanoparticles as gene vector. …”
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Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells by Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang

“…The results showed that the truncated, EF-1α first, chimeric, and β-globin introns can significantly improve stable transgene expression in CHO cells. The qPCR results indicated that the mRNA level of transgene increased through optimizing intron sequences combined with the CMV promoter. …”
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Improved growth performance, food efficiency, and lysine availability in growing rats fed with lysine-biofortified rice by Qing-Qing Yang, Pui Kit Suen, Chang-Quan Zhang, Wan Sheung Mak, Ming-Hong Gu, Qiao-Quan Liu, Samuel Sai-Ming Sun

“…By using a combined enhancement of lysine synthesis and suppression of its catabolism, we had produced two transgenic rice lines HFL1 and HFL2 (High Free Lysine) containing high concentration of free lysine. …”
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Development of cell lines with increased susceptibility to diverse adeno-associated viral vectors to enable in vitro potency assays by James Zengel, Emma S. Esterman, Anitha Ponnuswami, Nicholas R. Wall, Jan E. Carette

“…Robust cell-based assays that demonstrate and quantify the potency of AAV vectors in expressing their transgene are needed for clinical development. However, many AAV clinical serotypes poorly transduce cells in vitro and often contain cell-type-specific promoters inactive in commonly used cell lines. …”
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Structure of an ex vivoDrosophila TOM complex determined by single-particle cryoEM by Agalya Periasamy, Pamela Ornelas, Thomas Bausewein, Naomi Mitchell, Jiamin Zhao, Leonie M. Quinn, Werner Kuehlbrandt, Jacqueline M. Gulbis

“…The core TOM complex extracted from native membranes of the D. melanogaster retina contains transgenic Tom40 co-assembled with four endogenous TOM components: Tom22, Tom5, Tom6 and Tom7. …”
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Agrobacterium-mediated transformation of <i>Nicotiana glauca</i> and <i>Nicotiana sylvestris</i> by G. V. Khafizova, T. V. Matveeva

“…Agrobacterium-mediated transformation is the most popular approach for obtaining transgenic plants nowadays. There are plenty of protocols developed for different plant species. …”
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Citrus transcription factor CsERF1 is involved in the response to citrus tristeza disease by Qi Chen, Fulin Yan, Jing Liu, Zhipeng Xie, Junyao Jiang, Jiamei Liang, Jing Chen, Huanhuan Wang, Jinxiang Liu

“…The CTV titer in RNAi-CsERF1 transgenic sweet orange plants significantly increased. …”
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