Versatile High-Throughput Platform for Focused Ultrasound In Vitro Application by Steffen H. Tretbar, Marc Fournelle, Christian Degel, Franz-Josef Becker, Peter Weber, Sarah Therre-Mohr, Wolfgang Bost, Lisa Landgraf, Andreas Melzer

“…Two different platform configurations were developed: (a) a low-intensity version with 96 individual transducers allowing dry coupling of the well plate; and (b) a high-intensity version with water cooling, supporting parallel sonication of 32 out of 96 wells. …”
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CDK14 regulates the development and repair of lung by Jian-wei Chen, Yu-xiang Wang, Rong-rong Gao, Lan-yue Ma, Jing Zhong, Jia-xin Yang, Zhao-hua Deng, Yu-yan Li, Xiao-ling Li, Ya-hai Shu, Wen-jing Guo, Zi-yuan Zhou, Xiao Yu Tian, Jinjin Ma, Yang Liu, Qi Chen

“…Through RNA-seq analysis of both endothelial and epithelial cells, we found that knockdown of Cdk14 controls the expression of signal transducers and activator of transcription 1 (STAT1) as well as associated genes in interferon signaling. …”
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Electrophysiologically calibrated optogenetic stimulation of dentate granule cells mitigates dendritic spine loss in denervated organotypic entorhino-hippocampal slice cultures by Tijana Hanauske, Carolin Christina Koretz, Tassilo Jungenitz, Jochen Roeper, Alexander Drakew, Thomas Deller

“…Our findings showed that ChR2-expressing cells generated action potentials (APs) or calcium transients in response to illumination but were otherwise functionally indistinguishable from non-transduced GCs within the same neural circuit. However, the threshold for AP firing in single GCs varied based on the stimulus light intensity and the expression levels of ChR2. …”
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Development and Characterisation of a Novel NF-κB Reporter Cell Line for Investigation of Neuroinflammation by Marie-Theres Zeuner, Thomas Vallance, Sakthivel Vaiyapuri, Graeme S. Cottrell, Darius Widera

“…We used lentivirus to transduce the glioma cell line U251-MG with a tandem NF-κB reporter construct containing GFP and firefly luciferase allowing an assessment of NF-κB activity via fluorescence microscopy, flow cytometry, and luminometry. …”
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Evaluation of the Influence of Sensor Geometry and Physical Parameters on Impedance-Based Structural Health Monitoring by L.V. Palomino, K.M. Tsuruta, J.R.V. Mour Jr, D.A. Radea, V. Steffen Jr., D.J. Inman

“…This method measures the variation of the electromechanical impedance of the structure as caused by the presence of damage by using piezoelectric transducers bonded on the surface of the structure (or embedded into it). …”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…Biosensors harness biological materials as receptors linked to transducers, enabling the capture and transformation of primary biorecognition signals into measurable outputs. …”
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Current utility, instruments, and future directions for intra-renal pressure management during ureteroscopy: scoping review by global research in intra-renal pressure collaborative... by Steffi Kar Kei Yuen, Wen Zhong, Yun Sang Chan, Daniele Castellani, Naeem Bhojani, Madhu Sudan Agarwal, Theodoros Tokas, Stefanie Croghan, Helene Jung, Thomas Herrmann, Bhaskar Somani, Vineet Gauhar

“…Current noninvasive IRP monitoring devices include ureteric catheters placed retrogradely or via percutaneous tubing and connected to pressure transducers, amplified by cardiology-used pressure sensing systems or urodynamic systems, automated pressure-regulating systems, pressure sensing guidewires, and IRP sensing flexible ureteroscopes. …”
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Inhibition of hippocampal interleukin-6 receptor-evoked signalling normalises long-term potentiation in dystrophin-deficient mdx mice by Kimberley A. Stephenson, Aaron Barron, Mark G. Rae, Dervla O'Malley

“…Duchenne muscular dystrophy (DMD), an X-linked neuromuscular disorder, characterised by progressive immobility, chronic inflammation and premature death, is caused by the loss of the mechano-transducing signalling molecule, dystrophin. In non-contracting cells, such as neurons, dystrophin is likely to have a functional role in synaptic plasticity, anchoring post-synaptic receptors. …”
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Umbilical Cord Mesenchymal Stem Cells Overexpressing Heme Oxygenase-1 Promotes Symptoms Recovery in Cystitis Rats by Alleviating Neuroinflammation by Qiongqiong Gao, Zhentao Gao, Minzhi Su, Yong Huang, Chi Zhang, Cuiping Li, Hailun Zhan, Bolong Liu, Xiangfu Zhou

“…HUMSCs were capable of being transduced to overexpress HO-1. Injection of hUMSCs overexpressing HO-1 was more effective than hUMSCs alone in alleviating behavioral symptoms in rats. …”
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Experimental Study on the Flow Regimes and Pressure Gradients of Air-Oil-Water Three-Phase Flow in Horizontal Pipes by Luai M. Al-Hadhrami, S. M. Shaahid, Lukman O. Tunde, A. Al-Sarkhi

“…The flow patterns were observed and recorded using high speed video camera while the pressure drops were measured using pressure transducers and U-tube manometers. The flow patterns show strong dependence on water fraction, gas velocities, and liquid velocities. …”
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Experimental evaluation of mechanically stabilized earth walls reinforced with glass fiber reinforced polymer bars embedded in marble waste-sand mixtures by Shabir Hussain, Irshad Ahmad, Irfan Jamil, Fasih Ahmed Khan, Mansoor Ullah, Saif ul Islam, Mudassir Iqbal, R. Nawaz

“…FD and VS of the wall were measured by placing five Linear Vertical Displacement Transducers (LVDTs) on wall facing and five LVDTs on top of the wall at various strategic locations. …”
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Excitation and Propagation of Longitudinal L (0, 2) Mode Ultrasonic Guided Waves for the Detection of Damages in Hexagonal Pipes: Numerical and Experimental Studies by Xiang Wan, Meiru Liu, Xuhui Zhang, Hongwei Fan, Qinghua Mao, Ming Dong, Xing Wang, Hongwei Ma

“…A ring of piezoelectric transducers (PZTs) with the size of 25 mm × 5 mm ×0.5 mm is able to maximize the amplitude of L (0, 2) mode and successfully suppress the undesired L (0, 1) mode in the experiments. …”
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Features of the Surface and Subsurface Waves Application for Ultrasonic Evaluation of Physicomechanical Properties of Solids. Part 2. Strenghtned Inhomogeneous Surface Layer by A. R. Baev, A. L Mayorov, N. V. Levkovich, M. V. Asadchaya

“…The dependence connecting the wave velocity, its frequency, the depth of the hardened layer and the spatial distribution of hardness represented as a step with a changing slope of its side surface simulating the transition zone of the hardened layer are calculated.Using the pulse method and low-aperture transducers with a frequency of 1−3.8 MHz, the dependences of the surface wave velocity on the cutting height of a layer hardened by HDTV hardening are obtained. …”
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Electrochemical properties of hydroxyapatite immobilization material for potential cytosensor fabrication by Dennis Adusei, Bernard O. Asimeng, Francis D. Krampa, Elvis K. Tiburu

“…Aim: The biorecognition unit of an electrochemical biosensor requires molecules that are immobilised to serve as a bridge between the recognition unit and the transducing surface. Unique materials that enhance immobilisation of biorecognition molecules and improve electrochemical signal transduction are important in overcoming challenges based on the sensitivity of biosensors. …”
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Genetic mechanisms of the influence of light and phototransduction on Drosophila melanogaster lifespan by I. A. Solovev, M. V. Shaposhnikov, A. A. Moskalev

“…In the present paper, basic transducers of light and other signals, such as the family of TRP receptors, G proteins, phospholipase C, and others, are considered in the context of aging and longevity. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…We also developed an eGFP-Fluc2 construct in the Retro-X retroviral vector (Retro-X-eGFP-Fluc2). MSCs were transduced with retroviruses to generate CXCR4-overexpressing MSCs (MSC-CXCR4/Fluc2) and MSCs (MSC/Fluc2). …”
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Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations by Lucas Tricoli, Sunetra Sase, Julia L. Hacker, Vi Pham, Maxwell Chappell, Laura Breda, Stephanie N. Hurwitz, Naoto Tanaka, Carlo Castruccio Castracani, Amaliris Guerra, Zhongqi Hou, Lars Schlotawa, Karthikeyan Radhakrishnan, Matthew Hogenauer, Aoife Roche, John Everett, Frederic Bushman, Peter Kurre, Rebecca Ahrens-Nicklas, Laura A. Adang, Adeline L. Vanderver, Stefano Rivella

“…Three-month-old Arsa-knockout (KO) MLD mice transplanted with Arsa-KO bone marrow (BM) cells transduced with 0.6 VCN of EA1 demonstrated behavior and CNS histology matching wild-type (WT) mice. …”
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Identification and subcellular localization of proteins that interact with Duck plague virus pUL14 in infected host cells by Jieyu Wan, Mingshu Wang, Anchun Cheng, Wei Zhang, Qiao Yang, Bin Tian, Xumin Ou, Di Sun, Yu He, Xinxin Zhao, Ying Wu, Shaqiu Zhang, Juan Huang, Zhen Wu, YanLing Yu, Ling Zhang, Dekang Zhu, Renyong Jia, Mafeng Liu, Shun Chen

“…In addition, four viral proteins of interest, the α-gene transducing factor (α-TIF) pUL48, the nuclear egress complex (NEC) proteins pUL31/34, and pUL51, a protein involved in secondary envelopment, were validated by coimmunoprecipitation (co-IP) to interact with DPV pUL14. …”
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Tuning Electrical Conductivity and Ultrafast Optical Nonlinearity of Reduced-GO Films Ablated by Femtosecond Laser Direct Writing by Youliang Tao, Xuefeng Zhang, Han Wang, Zhongquan Nie, Deng Pan

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Endothelial and neuronal engagement by AAV-BR1 gene therapy alleviates neurological symptoms and lipid deposition in a mouse model of Niemann-Pick type C2 by Charlotte Laurfelt Munch Rasmussen, Signe Frost Frederiksen, Christian Würtz Heegaard, Maj Schneider Thomsen, Eva Hede, Bartosz Laczek, Jakob Körbelin, Daniel Wüstner, Louiza Bohn Thomsen, Markus Schwaninger, Ole N. Jensen, Torben Moos, Annette Burkhart

“…The vector exerted tropism for BECs and neurons resulting in a widespread NPC2 distribution in the brain with a concomitant reduction of cholesterol in adjacent neurons, presumably not transduced by the vector. Mass spectrometry imaging revealed distinct lipid alterations in the brains of Npc2−/− mice, with increased GM2 and GM3 ganglioside accumulation in the cerebellum and hippocampus. …”
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