Optimized for routine: highly sensitive fluorescent Telomeric Repeat Amplification Protocol (f-TRAP) by Silke Fähnrich, Anne Wedemann, Laura Steenpass, Wilhelm Gerhard Dirks

Subjects: Get full text

Proinflammatory Cytokines IL-6 and TNF-α Increased Telomerase Activity through NF-κB/STAT1/STAT3 Activation, and Withaferin A Inhibited the Signaling in Colorectal Cancer Cells by Seyung S. Chung, Yong Wu, Quincy Okobi, Debbie Adekoya, Mohammad Atefi, Orette Clarke, Pranabananda Dutta, Jaydutt V. Vadgama

“…The binding of STAT3 to the hTERT promoter region and telomerase activity showed reduction with withaferin A treatments. …”
Get full text

TERT de novo mutation-associated dyskeratosis congenita and porto-sinusoidal vascular disease: a case report by Ge Yu, Guijie Xin, Xu Liu, Wanyu Li, Chen Shao, Runping Gao

Subjects: Get full text

Telomere length as a quantitative trait: genome-wide survey and genetic mapping of telomere length-control genes in yeast. by Tonibelle Gatbonton, Maria Imbesi, Melisa Nelson, Joshua M Akey, Douglas M Ruderfer, Leonid Kruglyak, Julian A Simon, Antonio Bedalov

“…Genes that regulate telomere length span a variety of functions that can be broadly separated into telomerase-dependent and telomerase-independent pathways. …”
Get full text

Major Depressive Disorder and Measures of Cellular Aging: An Integrative Review by Patricia Anne Kinser, Debra E. Lyon

“…To examine the accelerated aging hypothesis, one of the biomarkers under study is leukocyte telomeres, and specifically the measure of telomere length and telomerase activity. This review integrates findings from eleven human studies which evaluated telomere length and telomerase activity, in order to synthesize the state of the current science and to inform the development of new knowledge and enhance nursing research of depression using appropriate biobehavioral measures. …”
Get full text

Compilation and functional classification of telomere length-associated genes in humans and other animal species by E. V. Ignatieva, N. S. Yudin, D. M. Larkin

“…The human genes identified by several research groups in populations of different ethnic origins are the genes encoding telomerase components such as TERC and TERT as well as STN1 encoding the CST complex component. …”
Get full text

Integrated analysis of single-cell and bulk-RNA sequencing for the cellular senescence in prognosis of lung adenocarcinoma by Fengqiang Yu, Liangyu Zhang, Xun Zhang, Jianshen Zeng, Fancai Lai

“…When telomerase is inhibited, cancer cells’ senescence began, preventing them from growing indefinitely. …”
Get full text

siRNA-mediated inhibition of hTERT enhances the effects of curcumin in promoting cell death in precursor-B acute lymphoblastic leukemia cells: an in silico and in vitro study by Muhammad Hossein Ashoub, Ali Afgar, Alireza Farsinejad, Razieh Razavi, Samira Anvari, Ahmad Fatemi

“…Abstract This study investigates the interrelationship between human telomerase reverse transcriptase (hTERT) and ferroptosis in precursor-B (pre-B) acute lymphoblastic leukemia (ALL), specifically examining how hTERT modulation affects ferroptotic cell death pathways. …”
Get full text

The Long and Short of It: The Role of Telomeres in Fetal Origins of Adult Disease by Stephanie E. Hallows, Timothy R. H. Regnault, Dean H. Betts

“…This paper will explore the areas where telomere and telomerase biology can have significant effects on various tissues in the body in IUGR outcomes.…”
Get full text

Development and validation of a prognostic and drug sensitivity model for gastric cancer utilizing telomere-related genes by Xiaoxiao Li, Xiaoxuan Wang, Fuxiang Yu, Zhongguo Li, Daxin Chen, Yingxue Qi, Zhongyu Lu, Yaqin Liu, Dongsheng Chen, Yaoqiang Wu

“…Background: Gastric cancer (GC) poses a major global health challenge because of its unfavorable prognosis. Elevated telomerase activity has been linked to the rapid growth and invasiveness of GC tumors. …”
Get full text

Telomere Length Is Associated With Adverse Atrial Remodeling in Patients With Atrial Fibrillation by Kabita Pradhan, Balram Neupane, Paul Niehues, Martin Kirschner, Fabian Beier, Chao‐Chung Kuo, Erika Anneliese Hilbold, Christian Bär, Oana‐Maria Thoma, Maximilian Waldner, Margherita Vieri, Tim H. Brümmendorf, Vithurithra Tharmapalan, Wolfgang Wagner, Michael Kleines, Mahdi Emrani, Matthias Daniel Zink, Andreas Napp, Nikolaus Marx, Michael Gramlich

“…Clonal hematopoiesis of indeterminate potential was assessed by next generation sequencing. Telomerase reverse transcriptase knockout (Tert−/−) and telomerase RNA component knockout (Terc−/−) mice were used to investigate the mechanistic impact of telomere shortening on atrial remodeling. …”
Get full text

Mathematical model linking telomeres to senescence in Saccharomyces cerevisiae reveals cell lineage versus population dynamics by Anaïs Rat, Veronica Martinez Fernandez, Marie Doumic, Maria Teresa Teixeira, Zhou Xu

“…Here, we present a mathematical model of telomere shortening and replicative senescence in Saccharomyces cerevisiae which is quantitatively calibrated and validated using data of telomerase-deficient single cells. Simulations of yeast populations, where cells with varying proliferation capacities compete against each other, show that the distribution of telomere lengths of the initial population shapes population growth, especially through the distribution of cells’ shortest telomere lengths. …”
Get full text

Study on the Dynamic Biological Characteristics of Sca-1+ Hematopoietic Stem and Progenitor Cell Senescence by Shan Geng, Xin-Yi Mu, Xiong-Bin Chen, Ji-Ying Hou, Dao-Yong Jia, Chun-Yan Xu, Ya-Ping Wang

“…Interestingly, the telomere length and telomerase activity increased to a certain degree in the 6-month-old group. …”
Get full text

Sirtuins, Bioageing, and Cancer by D. McGuinness, D. H. McGuinness, J. A. McCaul, P. G. Shiels

“…Here, we discuss the current understanding of the role of Sirtuins in age-related diseases while taking a closer look at their roles and functions in maintaining genomic stability and their influence on telomerase and telomere function.…”
Get full text

Yoga: As a Transformative Approach to Addressing Male Infertility and Enhancing Reproductive Health in Men: A Narrative Review by Anjali Yadav, Prabhakar Tiwari, Rima Dada

“…In addition, yoga contributes to maintaining sperm telomere length through the regulation of seminal free radical levels and increased telomerase activity, which are crucial for optimal embryo cleavage and the development of high-quality blastocysts. …”
Get full text

Content Determination of Active Component in Huangqi Yinyanghuo Group and Its Effects on hTERT and Bcl-2 Protein in Osteosarcoma by Ying Tan, Lei Tan, Shuai Huang, Junfan Lu, Longtan Yu

“…To screen the optimal extraction process and content determination of active component of Huangqi Yinyanghuo group (HYG) and to study the effects of HYG on human telomerase reverse transcriptase (hTERT) and Bcl-2 protein in osteosarcoma (HOS) cells, providing the theoretical basis for clinical application of HYG in treatment of osteosarcoma, orthogonal design table L9(43) was used to design the extraction process of HYG, and icariin was taken as the investigation index to optimize the extraction process of HYG. 0.125, 0.25, 0.5, 1, 2, 4, and 8 μmol/L HYG were taken to act separately on logarithmic growth phase osteosarcoma HOS cells, CCK-8 assay was used to determine cell viability, and immunohistochemical SP assay was used to determine the expression of hTERT and Bcl-2 protein. …”
Get full text

Geroprotection from ergothioneine treatment in Drosophila melanogaster by improving intestinal barrier and activation of intestinal autophagy by Hongyu Pan, Qianwang Zheng, Yuan Zou, Guozhao Luo, Mingzhen Tu, Na Wang, Jingru Zhong, Liqiong Guo, Junfang Lin

“…Previous studies have demonstrated that ergothioneine can increase the lifespan of Drosophila melanogaster and Caenorhabditis elegans, and enhance telomerase activity. In present study, the metagenomics sequencing and quantitative real-time polymerase chain reaction analysis were employed to characterize the composition and diversity of gut microbiome in aging flies. …”
Get full text

小鼠端粒酶蛋白亚单位重组腺病毒载体的构建及鉴定 by 姜楠, 陆敏强, 李华, 蔡常洁, 杨扬, 许赤, 冯炼强, 陈规划

“…【目的】构建小鼠端粒酶蛋白亚单位（mouse telomerase reverse transcriptase，mTERT）基因重组腺病毒载体，为下一步研究其体外表达和动物实验研究提供基础。…”
Get full text

Establishment of Novel Limbus-Derived, Highly Proliferative ABCG2+/ABCB5+ Limbal Epithelial Stem Cell Cultures by Eung Kweon Kim, Ga-Hyun Lee, Boram Lee, Yong-Sun Maeng

“…In addition, cultured LESCs expressed high levels of the stem cell markers Sox2, Oct4, c-Myc, and Klf4, had high telomerase activity, and had stable, normal genomes. …”
Get full text

RTEL1 is upregulated in gastric cancer and promotes tumor growth by Chunyu Yang, Suzeng Wang, Ge Gao, Peiwen Xu, Mengyuan Qian, Yuan Yin, Surui Yao, Zhaohui Huang, Zehua Bian

“…Identifying novel genes and pathways involved in GC development is crucial for improving the therapeutic outcome. Regulator of Telomerase Length 1 (RTEL1) has been found to maintain telomere stability through its helicase activity, facilitating telomere reconstruction and repair. …”
Get full text