Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening by Wei Jin, Yexuan Deng, John E. La Marca, Emily J. Lelliott, Sarah T. Diepstraten, Christina König, Lin Tai, Valentina Snetkova, Kristel M. Dorighi, Luke Hoberecht, Millicent G. Hedditch, Lauren Whelan, Geraldine Healey, Dan Fayle, Kieran Lau, Margaret A. Potts, Moore Z. Chen, Angus P. R. Johnston, Yang Liao, Wei Shi, Andrew J. Kueh, Benjamin Haley, Jean-Philippe Fortin, Marco J. Herold

“…We further demonstrate successful in vivo gene editing, using normal and cancer-prone enAsCas12a stem cells to reconstitute the haematopoietic system of wild-type mice. We also present compact, genome-wide Cas12a knockout libraries, with four crRNAs per gene encoded across one (Scherzo) or two (Menuetto) vectors, and demonstrate the utility of these libraries across methodologies: in vitro enrichment and drop-out screening in lymphoma cells and immortalised fibroblasts, respectively, and in vivo screens to identify lymphoma-driving events. …”
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3D-AttenNet model can predict clinically significant prostate cancer in PI-RADS category 3 patients: a retrospective multicenter study by Jie Bao, Litao Zhao, Xiaomeng Qiao, Zhenkai Li, Yanting Ji, Yueting Su, Libiao Ji, Junkang Shen, Jiangang Liu, Jie Tian, Ximing Wang, Hailin Shen, Chunhong Hu

“…Abstract Purposes The presence of clinically significant prostate cancer (csPCa) is equivocal for patients with prostate imaging reporting and data system (PI-RADS) category 3. We aim to develop deep learning models for re-stratify risks in PI-RADS category 3 patients. …”
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Experimental Investigation of the Dependence of Accessible Porosity and Methane Sorption Capacity of Carbonaceous Shales on Particle Size by Garri Gaus, Anton Kalmykov, Bernhard M. Krooss, Reinhard Fink

“…Poor interconnectivity of the pore system and slow, diffusion-controlled transport will massively retard gas production. …”
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Association and multimodal model of retinal and blood-based biomarkers for detection of preclinical Alzheimer’s disease by Swetha Ravichandran, Peter J. Snyder, Jessica Alber, Charles F. Murchison, Lauren E. Chaby, Andreas Jeromin, Edmund Arthur

“…Blood samples were assessed for concentrations of Aβ42/Aβ40 ratio, NfL, p-tau181, and p-tau217 (ALZpath, Inc.) using Single molecule array (SIMOA) technology. The Spectralis II system (Heidelberg Engineering) was used to acquire macular centered Spectral Domain Optical Coherence Tomography (SD-OCT) images for evaluation of putative retinal gliosis surface area and macular retinal nerve fiber layer (mRNFL) thickness. …”
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Molecular determinants of Neu5Ac binding to a tripartite ATP independent periplasmic (TRAP) transporter by Parveen Goyal, KanagaVijayan Dhanabalan, Mariafrancesca Scalise, Rosmarie Friemann, Cesare Indiveri, Renwick CJ Dobson, Kutti R Vinothkumar, Subramanian Ramaswamy

“…We have previously reported the structures of SiaQM, a membrane-embedded component of the Haemophilus influenzae TRAP transport system, (Currie et al., 2024). However, none of the published structures contain Neu5Ac bound to SiaQM. …”
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Effect of Medication Management at Home via Pharmacist-Led Home Televisits: Protocol for a Cluster Randomized Controlled Trial by Sheikh Rubana Hossain, Akanksha N Samant, Briana C Balsamo, Chelsea E Hawley, Michael C Zanchelli, Carolyn Zhu, Maria D Venegas, Marina Robertson, Megan B McCullough, Judith L Beizer, Kenneth S Boockvar, Albert L Siu, Lauren R Moo, William W Hung

“…The pilot phase of the study was completed December 2021 with 20 veterans and was primarily informed by the Steinman model of the prescribing process adapted to include system- and provider-level factors. The last date of enrollment was August 6, 2021. …”
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Evaluating implementation of a hospital‐based cancer registry to improve childhood cancer care in low‐ and middle‐income countries by Melissa R. Maas, Allison Yang, Michele A. Muir, James B. Collins IV, Courtney Canter, Gevorg Tamamyan, Inam Chitsike, Francine Kouya, Kim Hoa Nguyen, Alia Ahmad, Ana Patricia Alcasabas, Yi‐Jin Gao, Kimberly J. Johnson, Gia Ferrara, Nickhill Bhakta, Benyam Muluneh

“…Jude Global Childhood Cancer Analytics Resource and Epidemiological Surveillance System (SJCARES) is a cloud‐based HBCR network facilitating quality data collection of pediatric cancer. …”
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Assessment of Birth Preparedness and Complication Readiness among Pregnant Women: A Cross-sectional Study by T Elamathi, K Ananthi, Rimi Singh

“…It has less affinity and strength of depressant effects on myocardial and Central Nervous System (CNS) vital centers in pharmacodynamic studies, along with a superior pharmacokinetic profile. …”
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Injecting hope: the potential of intratumoral immunotherapy for locally advanced and metastatic cancer by Marketa Skalickova, Marketa Skalickova, Katerina Hadrava Vanova, Ondrej Uher, Jindriska Leischner Fialova, Jindriska Leischner Fialova, Jindriska Leischner Fialova, Katerina Petrlakova, Michal Masarik, Michal Masarik, Michal Masarik, Michal Masarik, Zdeněk Kejík, Zdeněk Kejík, Pavel Martasek, Karel Pacak, Milan Jakubek, Milan Jakubek

“…Recently, immunotherapy has shown success in patients by harnessing important interactions between the immune system and cancer. However, many of these therapies lead to frequent side effects when administered systemically, prompting treatment modifications or discontinuation or, in severe cases, fatalities. …”
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Hubungan Diare dengan Status Gizi Balita di Kelurahan Lubuk Buaya Kecamatan Koto Tangah Kota Padang by Alania Rosari, Eka Agustia Rini, Masrul Masrul

“…Malnutrition may increase the risk of infectious disease because the immune system is decreased. Otherwise, infectious disease can also affect the nutritional status because of decreased food intake, malabsorption, and increased body catabolism. …”
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Abuse of patent rights for pharmaceuticals in wartime conditions by A.O. Kodynets, O.F. Doroshenko, I.P. Volynets, G.K. Dorozhko, V.O. Petrenko, V.V. Belitsky

“…The study employs general scientific and specialized methods of scientific cognition, including dialectical, formal-logical, system-structural, comparative-legal methods, as well as modeling, analysis, and synthesis. …”
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Frequency and factors associated with loss to follow-up in newly diagnosed rheumatoid arthritis patient: a single-centre study by Sumariyono Sumariyono, Rudy Hidayat, Faisal Parlindungan, Suryo Anggoro Kusumo Wibowo, Anna Ariane, Johanda Damanik, Abirianty Priandani Araminta, Ryzkianty Annis Nurdin

“…The reasons for LTFU were distance from house to hospital constraints (76%), busy (56%), transportation constraints (38%), dissatisfaction with the outpatient clinic service (21%), lack of information about their disease (18%), having other comorbidities that compelled them to go to another department’s clinic (15%), difficulties understanding the clinic registration flow system (9%), and having minimal symptoms (6%). Using the c 2 test, we found that transportation constraints and busyness were significantly different between LTFU and routinely followed up patients ( p -value 0.008 and 0.200, respectively). …”
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Identification and functional analysis of hub genes involved in deoxynivalenol-induced enterotoxicity in porcine (Sus scrofa) by Jinhua He, Geng Zhao, Mingxia Chen, Ximing Ren, Peizhi Zhu, Zhizhong Liu, Jiayi Zhou, Hanwei Chen, Chuqiao Xiao, Xiang-Guang Li

“…The porcine digestive system is physiologically similar to that of humans, making pigs a suitable model for studying DON-induced enterotoxicity. …”
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Life cycle assessment of coconut plantation, copra, and charcoal production by T. Puspaningrum, N.S. Indrasti, C. Indrawanto, M. Yani

“…Life cycle assessment is a tool for evaluating the inputs, outputs, and potential impacts of a product system throughout its life cycle and is associated with product sustainability. …”
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Therapeutic Potential of Clove Oil in Mitigating Cadmium-Induced Hepatorenal Toxicity Through Antioxidant, Anti-Inflammatory, and Antiapoptotic Mechanisms by Inas M. Elgharib, Fatma M. Abdelhamid, Gehad E. Elshopakey, Hatem Sembawa, Talat A. Albukhari, Waheed A. Filimban, Rehab M. Bagadood, Mohamed E. El-Boshy, Engy F. Risha

“…Conclusions: Hematological and biochemical parameters, as well as the antioxidant system, improved following clove oil treatment, leading to a reduction in hepatorenal damage. …”
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Place-based household vouchers for locally supplied fruit and vegetables: the Fresh Street pilot cluster randomised controlled trial by C. Relton, M. K. Blake, G. Bridge, D. Umney, S. J. C. Taylor, J. Adams, B. Mihaylova, C. Griffiths, R. Hooper, R. Phillips, L. Palmer, A. Gamston, K. Williamson

“…Conclusions This was the first pilot RCT of a place-based, household voucher approach. The newly developed system for securely printing and redeeming the vouchers worked well and is potentially scalable. …”
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Insights into the mechanisms of serplulimab: a distinctive anti-PD-1 monoclonal antibody, in combination with a TIGIT or LAG3 inhibitor in preclinical tumor immunotherapy studies by Yizhou Zhang, Ruicheng Wei, Ge Song, Xinyi Yang, Mengli Zhang, Wei Liu, Aiying Xiong, Xuehan Zhang, Qianhao Li, Wan-Jen Yang, Chencheng Han, Rui Liu, Chen Hu, Qingyu Wang, Jun Zhu, Yongqiang Shan

“…Mechanistically, the serplulimab combination effectively reduces tumor microenvironment Treg cell populations, augments effector and memory T cell populations, and more potently modulates genes associated with diverse facets of the immune system, surpassing the effects of the pembrolizumab combination. …”
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Selected cannabis cultivars modulate glial activation: in vitro and in vivo studies by Sigal Fleisher-Berkovich, Nitzan Sharon, Yvonne Ventura, Valeria Feinshtein, Jonathan Gorelick, Nirit Bernstein, Shimon Ben-Shabat

“…Abstract Introduction Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system characterized by neuroinflammation, demyelination and axonal loss. …”
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Automated method of bronchus and artery dimension measurement in an adult bronchiectasis population by Angelina L.P. Pieters, Qianting Lv, Jennifer J. Meerburg, Tjeerd van der Veer, Eleni-Rosalina Andrinopoulou, Pierluigi Ciet, James D. Chalmers, Michael R. Loebinger, Charles S. Haworth, J. Stuart Elborn, Harm A.W.M. Tiddens

“…CTs were analysed manually with the BEST-CT scoring system and automatically using LungQ (v.2.1.0.1, Thirona, The Netherlands), which measures the following BA dimensions: diameters of bronchial outer wall (Bout), bronchial inner wall (Bin) and artery (A), and bronchial wall thickness (Bwt) and computes BA ratios (Bout/A and Bin/A) to assess bronchial widening. …”
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Detection and Classification of Teacher-Rated Children’s Activity Levels Using Millimeter-Wave Radar and Machine Learning: A Pilot Study in a Real Primary School Environment by Tianyi Wang, Takuya Sakamoto, Yu Oshima, Itsuki Iwata, Masaya Kato, Haruto Kobayashi, Manabu Wakuta, Masako Myowa, Tokomo Nishimura, Atsushi Senju

“…The experiment involves a nine-day observational study, using two radar systems to monitor the activities of 14 children in a primary school. …”
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